acc 029  (Alomone Labs)


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    Alomone Labs acc 029
    Acc 029, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acc 029/product/Alomone Labs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    acc 029 - by Bioz Stars, 2022-12
    94/100 stars

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  • 94
    Alomone Labs anti rat trpv1 channel atto 488
    <t>TRPV1</t> and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 <t>channel</t> in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P
    Anti Rat Trpv1 Channel Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rat trpv1 channel atto 488/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rat trpv1 channel atto 488 - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs rat trpv1
    Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 <t>(TRPV1)</t> immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic <t>TRPV1-IR.</t> The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.
    Rat Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat trpv1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat trpv1 - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs rabbit anti trpv1
    Paclitaxel increased immunoreactivity for <t>TRPV1</t> and TRPM8 in sensory neurons. Immunocytochemical staining of cultured DRG neurons treated with vehicle or paclitaxel using <t>anti‐TRPV1,</t> anti‐TRPA1 or anti‐TRPM8 antibodies along with the neuronal marker NeuN and DAPI staining. Green: TRPV1 (a, b), TRPA1 (d, e) or TRPM8 (g and h). Red: NeuN. DAPI: cyan. Scale bar denotes 20 μm. (c, f and i) Mean fluorescence intensity of TRPV1 (c), TRPA1 (f) or TRPM8 (i) immunoreactivity for vehicle and paclitaxel treated neurons. Data were collected 48 h post‐treatment. Data are presented as individual values with means ± SD; N = 7. * P
    Rabbit Anti Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti trpv1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti trpv1 - by Bioz Stars, 2022-12
    94/100 stars
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    Image Search Results


    TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P

    Journal: Journal of Inflammation Research

    Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats

    doi: 10.2147/JIR.S367193

    Figure Lengend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P

    Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies: anti-rat TRPV1 channel ATTO-488 (1:240, cat.ACC-029-AG, Alomone labs, Jerusalem), anti-rat TRPA1 channel (1:240, cat.ACC-037, Alomone labs, Jerusalem), anti-rat protein gene product 9.5 (PGP 9.5, 1:500, NB300-676, Novus Biological), rabbit anti-Chymase (1:250, NBP2-257441, Novus Biological) and mouse anti-Tryptase (1:250, NBP2-26444, Novus Biological).

    Techniques: Expressing, Staining, Fluorescence

    TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P

    Journal: Journal of Inflammation Research

    Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats

    doi: 10.2147/JIR.S367193

    Figure Lengend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P

    Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies: anti-rat TRPV1 channel ATTO-488 (1:240, cat.ACC-029-AG, Alomone labs, Jerusalem), anti-rat TRPA1 channel (1:240, cat.ACC-037, Alomone labs, Jerusalem), anti-rat protein gene product 9.5 (PGP 9.5, 1:500, NB300-676, Novus Biological), rabbit anti-Chymase (1:250, NBP2-257441, Novus Biological) and mouse anti-Tryptase (1:250, NBP2-26444, Novus Biological).

    Techniques: Expressing, Staining, Fluorescence

    Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P

    Journal: Journal of Inflammation Research

    Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats

    doi: 10.2147/JIR.S367193

    Figure Lengend Snippet: Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P

    Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies: anti-rat TRPV1 channel ATTO-488 (1:240, cat.ACC-029-AG, Alomone labs, Jerusalem), anti-rat TRPA1 channel (1:240, cat.ACC-037, Alomone labs, Jerusalem), anti-rat protein gene product 9.5 (PGP 9.5, 1:500, NB300-676, Novus Biological), rabbit anti-Chymase (1:250, NBP2-257441, Novus Biological) and mouse anti-Tryptase (1:250, NBP2-26444, Novus Biological).

    Techniques: Expressing, Staining, Fluorescence

    Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPV1-IR. Bar, 50 μm.

    Article Snippet: The immunogen of the rabbit anti-TRPV1 (ACC-030) was the peptide (C)EDAEVFK DSMVPGEK (824–838) of rat TRPV1.

    Techniques: Labeling

    Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Quantification of the intensity of the expression of CB1R (a) , CB2R (b) , GPR55 (c) , PPARα (d) , TRPV1 (e) , TRPA1 (f) , 5-HT1aR (g) , in the suprabasal layers of 7 CTRL- and 8 AD-dogs. Data are represented as Mean ± SD and were analyzed using the Student T -test. P

    Article Snippet: The immunogen of the rabbit anti-TRPV1 (ACC-030) was the peptide (C)EDAEVFK DSMVPGEK (824–838) of rat TRPV1.

    Techniques: Expressing

    Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.

    Journal: Frontiers in Veterinary Science

    Article Title: Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis

    doi: 10.3389/fvets.2022.915896

    Figure Lengend Snippet: Photomicrographs of cryosections of canine skin showing transient receptor potential ankyrin 1 (TRPA1) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f) . (a–c) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the CTRL dogs showing moderate cytoplasmic TRPV1-IR. The TRPA1 immunolabelling was brighter in the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing moderate TRPV1-IR. (d–f) The white arrows indicate some DAPI-labeled nuclei of the keratinocytes of the AD dogs showing bright cytoplasmic TRPV1-IR. Not all the cells of the suprabasal layer showed the same degree of TRPA1-IR which was brighter in the cytoplasm of the more superficial cells. The open arrows indicate some DAPI-labeled nuclei of the dermal cells showing bright TRPA1-IR. Bar, 50 μm.

    Article Snippet: The immunogen of the rabbit anti-TRPV1 (ACC-030) was the peptide (C)EDAEVFK DSMVPGEK (824–838) of rat TRPV1.

    Techniques: Labeling

    Paclitaxel increased immunoreactivity for TRPV1 and TRPM8 in sensory neurons. Immunocytochemical staining of cultured DRG neurons treated with vehicle or paclitaxel using anti‐TRPV1, anti‐TRPA1 or anti‐TRPM8 antibodies along with the neuronal marker NeuN and DAPI staining. Green: TRPV1 (a, b), TRPA1 (d, e) or TRPM8 (g and h). Red: NeuN. DAPI: cyan. Scale bar denotes 20 μm. (c, f and i) Mean fluorescence intensity of TRPV1 (c), TRPA1 (f) or TRPM8 (i) immunoreactivity for vehicle and paclitaxel treated neurons. Data were collected 48 h post‐treatment. Data are presented as individual values with means ± SD; N = 7. * P

    Journal: British Journal of Pharmacology

    Article Title: Paclitaxel in vitro reversibly sensitizes the excitability of IB4(−) and IB4(+) sensory neurons from male and female rats). Paclitaxel in vitro reversibly sensitizes the excitability of IB4(−) and IB4(+) sensory neurons from male and female rats

    doi: 10.1111/bph.15809

    Figure Lengend Snippet: Paclitaxel increased immunoreactivity for TRPV1 and TRPM8 in sensory neurons. Immunocytochemical staining of cultured DRG neurons treated with vehicle or paclitaxel using anti‐TRPV1, anti‐TRPA1 or anti‐TRPM8 antibodies along with the neuronal marker NeuN and DAPI staining. Green: TRPV1 (a, b), TRPA1 (d, e) or TRPM8 (g and h). Red: NeuN. DAPI: cyan. Scale bar denotes 20 μm. (c, f and i) Mean fluorescence intensity of TRPV1 (c), TRPA1 (f) or TRPM8 (i) immunoreactivity for vehicle and paclitaxel treated neurons. Data were collected 48 h post‐treatment. Data are presented as individual values with means ± SD; N = 7. * P

    Article Snippet: Next, membranes were incubated at 4°C overnight with the following primary antibodies diluted in 1% BSA in TBST: rabbit anti‐Actin, 1:1000 (Sigma‐Aldrich, Cat# A2066, RRID:AB_476693); rabbit anti‐TRPV1, 1:1000 (Alomone Labs, Cat# ACC‐029); rabbit anti‐TRPM8, 1:500 (Alomone Labs, Cat# ACC‐049); rabbit anti‐NaV 1.7, 1:2500 (Alomone Labs, Cat# ASC‐008); and rabbit anti‐NaV1.8, 1:1000 (Alomone Labs, Cat# ASC‐016), respectively.

    Techniques: Staining, Cell Culture, Marker, Fluorescence

    Paclitaxel increases TRPV1 and TRPM8 channel activity in IB4(−) neurons. (a, c and e) Representative inward current responses to a 1 μM capsaicin (a), 100 μM AITC (c) and 100 μM menthol (e) of IB4(−) and IB4(+) neurons exposed to vehicle or paclitaxel. Calibration bars are for all recordings. TRPM8 channel activity was further confirmed by using the antagonist 10 μM AMTB along with the menthol pulse. (b, d, and f) Peak current density observed after application of capsaicin (b), AITC (d) or menthol (f). AITC current was evoked in the same cells as capsaicin, after application of 4 capsaicin pulses. To quantify TRPM8 responses the remaining inward current during co‐application of menthol and AMTB was subtracted from the peak response to menthol. Data are expressed as medians (with interquartile range) or as mean ± SD. * P

    Journal: British Journal of Pharmacology

    Article Title: Paclitaxel in vitro reversibly sensitizes the excitability of IB4(−) and IB4(+) sensory neurons from male and female rats). Paclitaxel in vitro reversibly sensitizes the excitability of IB4(−) and IB4(+) sensory neurons from male and female rats

    doi: 10.1111/bph.15809

    Figure Lengend Snippet: Paclitaxel increases TRPV1 and TRPM8 channel activity in IB4(−) neurons. (a, c and e) Representative inward current responses to a 1 μM capsaicin (a), 100 μM AITC (c) and 100 μM menthol (e) of IB4(−) and IB4(+) neurons exposed to vehicle or paclitaxel. Calibration bars are for all recordings. TRPM8 channel activity was further confirmed by using the antagonist 10 μM AMTB along with the menthol pulse. (b, d, and f) Peak current density observed after application of capsaicin (b), AITC (d) or menthol (f). AITC current was evoked in the same cells as capsaicin, after application of 4 capsaicin pulses. To quantify TRPM8 responses the remaining inward current during co‐application of menthol and AMTB was subtracted from the peak response to menthol. Data are expressed as medians (with interquartile range) or as mean ± SD. * P

    Article Snippet: Next, membranes were incubated at 4°C overnight with the following primary antibodies diluted in 1% BSA in TBST: rabbit anti‐Actin, 1:1000 (Sigma‐Aldrich, Cat# A2066, RRID:AB_476693); rabbit anti‐TRPV1, 1:1000 (Alomone Labs, Cat# ACC‐029); rabbit anti‐TRPM8, 1:500 (Alomone Labs, Cat# ACC‐049); rabbit anti‐NaV 1.7, 1:2500 (Alomone Labs, Cat# ASC‐008); and rabbit anti‐NaV1.8, 1:1000 (Alomone Labs, Cat# ASC‐016), respectively.

    Techniques: Activity Assay