microplate reader plate  (Azure Biosystems)

Journal: International Journal of Molecular Sciences

Article Title: Targeting Lysosomes to Reverse Hydroquinone-Induced Autophagy Defects and Oxidative Damage in Human Retinal Pigment Epithelial Cells

doi: 10.3390/ijms22169042

Figure Lengend Snippet: ARPE-19 cells are vulnerable to hydroquinone (HQ)-induced oxidative damage. ( A ) Live-cell confocal microscopy for intracellular reactive oxygen species (ROS) using CM-H2DCFDA dye after incubation of cells with HQ or H 2 O 2 for 2 h. Basal ROS level in the control is barely visible compared to H 2 O 2 treatment or HQ upon calibration. ( B ) Quantification of the (i) cell viability by Trypan blue dye exclusion assay, (ii) ROS levels, and (iii) protein carbonyl levels by fluorescent spectrometry using a microplate reader following incubation with HQ or H 2 O 2 for 2 h. Data represent the mean (+standard deviation, SD) of 3 independent experiments of 3 replicates each. Statistical analysis was performed by one-way ANOVA followed by Dunnett’s multiple comparison tests. * p

Article Snippet: Finally, samples were incubated with an anti-DNP antibody followed by HRP conjugated secondary antibody, and the absorbance was measured at 450 nm wavelength using a microplate Reader plate (Ao, Azure Biosystems Inc., Dublin, CA, USA).

Techniques: Confocal Microscopy, Incubation, Exclusion Assay, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Targeting Lysosomes to Reverse Hydroquinone-Induced Autophagy Defects and Oxidative Damage in Human Retinal Pigment Epithelial Cells

doi: 10.3390/ijms22169042

Figure Lengend Snippet: Effects of hydroquinone (HQ) and H 2 O 2 on mitochondrial morphology and mitochondrial membrane potential. ( A ) Live-cell fluorescence microscopy with the MitoTracker Green FM dye to determine changes in mitochondrial morphology in cells after incubation with HQ or H 2 O 2 for 2 h. ( B ) Measurement of mitochondrial membrane potential using TMRE dye in cells after treatment with HQ or H 2 O 2 for 2 h and using a fluorescence microplate reader at excitation/emission of 549 nm/575 nm. Data represent the mean (+standard deviation, SD) of 3 independent experiments of 3 replicates each. Statistical analysis was performed by one-way ANOVA followed by Dunnett’s multiple comparison tests. * p

Article Snippet: Finally, samples were incubated with an anti-DNP antibody followed by HRP conjugated secondary antibody, and the absorbance was measured at 450 nm wavelength using a microplate Reader plate (Ao, Azure Biosystems Inc., Dublin, CA, USA).

Techniques: Fluorescence, Microscopy, Incubation, Standard Deviation