2 3 eda m7 gtp  (Jena Bioscience)


Bioz Verified Symbol Jena Bioscience is a verified supplier
Bioz Manufacturer Symbol Jena Bioscience manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Name:
    Immobilized 2 3 EDA m7GTP
    Description:

    Catalog Number:
    AC-142L
    Price:
    916.9
    Category:
    Affinity Chromatography
    Size:
    5 ml
    Buy from Supplier


    Structured Review

    Jena Bioscience 2 3 eda m7 gtp

    https://www.bioz.com/result/2 3 eda m7 gtp/product/Jena Bioscience
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 3 eda m7 gtp - by Bioz Stars, 2021-06
    92/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: DAP5 associates with eIF2β and eIF4AI to promote Internal Ribosome Entry Site driven translation
    Article Snippet: .. The lysate was mixed with 100 ul Cap-column beads (Immobilized 2′/3′-EDA-m7GTP; Jena Bioscience GmbH) that were previously washed with buffer D and incubated at 4°C for 1 h while mixing with rotation. ..

    Transfection:

    Article Title: XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis
    Article Snippet: Anti-mouse (7076) and anti-rabbit (7074) secondary antibodies (horseradish peroxidase-conjugated) were acquired from Cell Signaling Technology. .. Reagents and kits used in this study are listed as follows: Selinexor (KPT-330), MG-132, z-VAD-FMK (Selleck, Shanghai, China), A-1155463, A-1331852, Actinomycin D (Act D), (Medchem Express, Monmouth Junction, USA), PMSF, propidium iodide, PVP (Sigma-Aldrich, St. Louis, USA), the CalPhos Mammalian Transfection Kit (TaKaRa Bio, Kusatsu, Japan), protease inhibitor cocktail, RNase A, Click-iTTM RNA HCS Assays, Click-iT™ Plus OPP Protein Synthesis Assay Kit Alexa Fluor™ 488 picolyl azide, Lipofectamine 3000 Reagent, the PARIS™ Kit (Thermo Scientific, Waltham, USA), phosphatase inhibitor, thiazolyl blue tetrazolium bromide (Sangon, Shanghai, China), cell lysis buffer for western and IP, cycloheximide, the Mitochondrial Membrane Potential Assay Kit with JC-1, Nuclear and Cytoplasmic Protein Extraction Kit, RIPA (Beyotime, Nantong, China), LightCycler 480 SYBR Green I Master, protein A agarose, protein G agarose (Roche Diagnositics, Indianapolis, USA), Script Reverse Transcription Supermix (Bio-Rad, Berkeley, USA), immobilized 2′/3′-EDA-m7 GTP (Jena Bioscience, Jena,Germany), the Dual-Luciferase® Reporter Assay System (Promega Coporation, Madison, USA), Pluronic F-68 (Life technologies), and Phosal 50 PG (Lipoid, Ludwigshafen, German) were used in this study. .. Lentivirus-mediated gene transductionShort hairpin RNAs (shRNAs) targeting human 4E-BP1, Bax, Bak and Mcl-1, and a scrambled (control) shRNA were inserted into the lentiviral vector pLKD-CMV-GFP-U6-shRNA.

    Protease Inhibitor:

    Article Title: XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis
    Article Snippet: Anti-mouse (7076) and anti-rabbit (7074) secondary antibodies (horseradish peroxidase-conjugated) were acquired from Cell Signaling Technology. .. Reagents and kits used in this study are listed as follows: Selinexor (KPT-330), MG-132, z-VAD-FMK (Selleck, Shanghai, China), A-1155463, A-1331852, Actinomycin D (Act D), (Medchem Express, Monmouth Junction, USA), PMSF, propidium iodide, PVP (Sigma-Aldrich, St. Louis, USA), the CalPhos Mammalian Transfection Kit (TaKaRa Bio, Kusatsu, Japan), protease inhibitor cocktail, RNase A, Click-iTTM RNA HCS Assays, Click-iT™ Plus OPP Protein Synthesis Assay Kit Alexa Fluor™ 488 picolyl azide, Lipofectamine 3000 Reagent, the PARIS™ Kit (Thermo Scientific, Waltham, USA), phosphatase inhibitor, thiazolyl blue tetrazolium bromide (Sangon, Shanghai, China), cell lysis buffer for western and IP, cycloheximide, the Mitochondrial Membrane Potential Assay Kit with JC-1, Nuclear and Cytoplasmic Protein Extraction Kit, RIPA (Beyotime, Nantong, China), LightCycler 480 SYBR Green I Master, protein A agarose, protein G agarose (Roche Diagnositics, Indianapolis, USA), Script Reverse Transcription Supermix (Bio-Rad, Berkeley, USA), immobilized 2′/3′-EDA-m7 GTP (Jena Bioscience, Jena,Germany), the Dual-Luciferase® Reporter Assay System (Promega Coporation, Madison, USA), Pluronic F-68 (Life technologies), and Phosal 50 PG (Lipoid, Ludwigshafen, German) were used in this study. .. Lentivirus-mediated gene transductionShort hairpin RNAs (shRNAs) targeting human 4E-BP1, Bax, Bak and Mcl-1, and a scrambled (control) shRNA were inserted into the lentiviral vector pLKD-CMV-GFP-U6-shRNA.

    Lysis:

    Article Title: XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis
    Article Snippet: Anti-mouse (7076) and anti-rabbit (7074) secondary antibodies (horseradish peroxidase-conjugated) were acquired from Cell Signaling Technology. .. Reagents and kits used in this study are listed as follows: Selinexor (KPT-330), MG-132, z-VAD-FMK (Selleck, Shanghai, China), A-1155463, A-1331852, Actinomycin D (Act D), (Medchem Express, Monmouth Junction, USA), PMSF, propidium iodide, PVP (Sigma-Aldrich, St. Louis, USA), the CalPhos Mammalian Transfection Kit (TaKaRa Bio, Kusatsu, Japan), protease inhibitor cocktail, RNase A, Click-iTTM RNA HCS Assays, Click-iT™ Plus OPP Protein Synthesis Assay Kit Alexa Fluor™ 488 picolyl azide, Lipofectamine 3000 Reagent, the PARIS™ Kit (Thermo Scientific, Waltham, USA), phosphatase inhibitor, thiazolyl blue tetrazolium bromide (Sangon, Shanghai, China), cell lysis buffer for western and IP, cycloheximide, the Mitochondrial Membrane Potential Assay Kit with JC-1, Nuclear and Cytoplasmic Protein Extraction Kit, RIPA (Beyotime, Nantong, China), LightCycler 480 SYBR Green I Master, protein A agarose, protein G agarose (Roche Diagnositics, Indianapolis, USA), Script Reverse Transcription Supermix (Bio-Rad, Berkeley, USA), immobilized 2′/3′-EDA-m7 GTP (Jena Bioscience, Jena,Germany), the Dual-Luciferase® Reporter Assay System (Promega Coporation, Madison, USA), Pluronic F-68 (Life technologies), and Phosal 50 PG (Lipoid, Ludwigshafen, German) were used in this study. .. Lentivirus-mediated gene transductionShort hairpin RNAs (shRNAs) targeting human 4E-BP1, Bax, Bak and Mcl-1, and a scrambled (control) shRNA were inserted into the lentiviral vector pLKD-CMV-GFP-U6-shRNA.

    Western Blot:

    Article Title: XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis
    Article Snippet: Anti-mouse (7076) and anti-rabbit (7074) secondary antibodies (horseradish peroxidase-conjugated) were acquired from Cell Signaling Technology. .. Reagents and kits used in this study are listed as follows: Selinexor (KPT-330), MG-132, z-VAD-FMK (Selleck, Shanghai, China), A-1155463, A-1331852, Actinomycin D (Act D), (Medchem Express, Monmouth Junction, USA), PMSF, propidium iodide, PVP (Sigma-Aldrich, St. Louis, USA), the CalPhos Mammalian Transfection Kit (TaKaRa Bio, Kusatsu, Japan), protease inhibitor cocktail, RNase A, Click-iTTM RNA HCS Assays, Click-iT™ Plus OPP Protein Synthesis Assay Kit Alexa Fluor™ 488 picolyl azide, Lipofectamine 3000 Reagent, the PARIS™ Kit (Thermo Scientific, Waltham, USA), phosphatase inhibitor, thiazolyl blue tetrazolium bromide (Sangon, Shanghai, China), cell lysis buffer for western and IP, cycloheximide, the Mitochondrial Membrane Potential Assay Kit with JC-1, Nuclear and Cytoplasmic Protein Extraction Kit, RIPA (Beyotime, Nantong, China), LightCycler 480 SYBR Green I Master, protein A agarose, protein G agarose (Roche Diagnositics, Indianapolis, USA), Script Reverse Transcription Supermix (Bio-Rad, Berkeley, USA), immobilized 2′/3′-EDA-m7 GTP (Jena Bioscience, Jena,Germany), the Dual-Luciferase® Reporter Assay System (Promega Coporation, Madison, USA), Pluronic F-68 (Life technologies), and Phosal 50 PG (Lipoid, Ludwigshafen, German) were used in this study. .. Lentivirus-mediated gene transductionShort hairpin RNAs (shRNAs) targeting human 4E-BP1, Bax, Bak and Mcl-1, and a scrambled (control) shRNA were inserted into the lentiviral vector pLKD-CMV-GFP-U6-shRNA.

    Protein Extraction:

    Article Title: XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis
    Article Snippet: Anti-mouse (7076) and anti-rabbit (7074) secondary antibodies (horseradish peroxidase-conjugated) were acquired from Cell Signaling Technology. .. Reagents and kits used in this study are listed as follows: Selinexor (KPT-330), MG-132, z-VAD-FMK (Selleck, Shanghai, China), A-1155463, A-1331852, Actinomycin D (Act D), (Medchem Express, Monmouth Junction, USA), PMSF, propidium iodide, PVP (Sigma-Aldrich, St. Louis, USA), the CalPhos Mammalian Transfection Kit (TaKaRa Bio, Kusatsu, Japan), protease inhibitor cocktail, RNase A, Click-iTTM RNA HCS Assays, Click-iT™ Plus OPP Protein Synthesis Assay Kit Alexa Fluor™ 488 picolyl azide, Lipofectamine 3000 Reagent, the PARIS™ Kit (Thermo Scientific, Waltham, USA), phosphatase inhibitor, thiazolyl blue tetrazolium bromide (Sangon, Shanghai, China), cell lysis buffer for western and IP, cycloheximide, the Mitochondrial Membrane Potential Assay Kit with JC-1, Nuclear and Cytoplasmic Protein Extraction Kit, RIPA (Beyotime, Nantong, China), LightCycler 480 SYBR Green I Master, protein A agarose, protein G agarose (Roche Diagnositics, Indianapolis, USA), Script Reverse Transcription Supermix (Bio-Rad, Berkeley, USA), immobilized 2′/3′-EDA-m7 GTP (Jena Bioscience, Jena,Germany), the Dual-Luciferase® Reporter Assay System (Promega Coporation, Madison, USA), Pluronic F-68 (Life technologies), and Phosal 50 PG (Lipoid, Ludwigshafen, German) were used in this study. .. Lentivirus-mediated gene transductionShort hairpin RNAs (shRNAs) targeting human 4E-BP1, Bax, Bak and Mcl-1, and a scrambled (control) shRNA were inserted into the lentiviral vector pLKD-CMV-GFP-U6-shRNA.

    SYBR Green Assay:

    Article Title: XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis
    Article Snippet: Anti-mouse (7076) and anti-rabbit (7074) secondary antibodies (horseradish peroxidase-conjugated) were acquired from Cell Signaling Technology. .. Reagents and kits used in this study are listed as follows: Selinexor (KPT-330), MG-132, z-VAD-FMK (Selleck, Shanghai, China), A-1155463, A-1331852, Actinomycin D (Act D), (Medchem Express, Monmouth Junction, USA), PMSF, propidium iodide, PVP (Sigma-Aldrich, St. Louis, USA), the CalPhos Mammalian Transfection Kit (TaKaRa Bio, Kusatsu, Japan), protease inhibitor cocktail, RNase A, Click-iTTM RNA HCS Assays, Click-iT™ Plus OPP Protein Synthesis Assay Kit Alexa Fluor™ 488 picolyl azide, Lipofectamine 3000 Reagent, the PARIS™ Kit (Thermo Scientific, Waltham, USA), phosphatase inhibitor, thiazolyl blue tetrazolium bromide (Sangon, Shanghai, China), cell lysis buffer for western and IP, cycloheximide, the Mitochondrial Membrane Potential Assay Kit with JC-1, Nuclear and Cytoplasmic Protein Extraction Kit, RIPA (Beyotime, Nantong, China), LightCycler 480 SYBR Green I Master, protein A agarose, protein G agarose (Roche Diagnositics, Indianapolis, USA), Script Reverse Transcription Supermix (Bio-Rad, Berkeley, USA), immobilized 2′/3′-EDA-m7 GTP (Jena Bioscience, Jena,Germany), the Dual-Luciferase® Reporter Assay System (Promega Coporation, Madison, USA), Pluronic F-68 (Life technologies), and Phosal 50 PG (Lipoid, Ludwigshafen, German) were used in this study. .. Lentivirus-mediated gene transductionShort hairpin RNAs (shRNAs) targeting human 4E-BP1, Bax, Bak and Mcl-1, and a scrambled (control) shRNA were inserted into the lentiviral vector pLKD-CMV-GFP-U6-shRNA.

    Reporter Assay:

    Article Title: XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis
    Article Snippet: Anti-mouse (7076) and anti-rabbit (7074) secondary antibodies (horseradish peroxidase-conjugated) were acquired from Cell Signaling Technology. .. Reagents and kits used in this study are listed as follows: Selinexor (KPT-330), MG-132, z-VAD-FMK (Selleck, Shanghai, China), A-1155463, A-1331852, Actinomycin D (Act D), (Medchem Express, Monmouth Junction, USA), PMSF, propidium iodide, PVP (Sigma-Aldrich, St. Louis, USA), the CalPhos Mammalian Transfection Kit (TaKaRa Bio, Kusatsu, Japan), protease inhibitor cocktail, RNase A, Click-iTTM RNA HCS Assays, Click-iT™ Plus OPP Protein Synthesis Assay Kit Alexa Fluor™ 488 picolyl azide, Lipofectamine 3000 Reagent, the PARIS™ Kit (Thermo Scientific, Waltham, USA), phosphatase inhibitor, thiazolyl blue tetrazolium bromide (Sangon, Shanghai, China), cell lysis buffer for western and IP, cycloheximide, the Mitochondrial Membrane Potential Assay Kit with JC-1, Nuclear and Cytoplasmic Protein Extraction Kit, RIPA (Beyotime, Nantong, China), LightCycler 480 SYBR Green I Master, protein A agarose, protein G agarose (Roche Diagnositics, Indianapolis, USA), Script Reverse Transcription Supermix (Bio-Rad, Berkeley, USA), immobilized 2′/3′-EDA-m7 GTP (Jena Bioscience, Jena,Germany), the Dual-Luciferase® Reporter Assay System (Promega Coporation, Madison, USA), Pluronic F-68 (Life technologies), and Phosal 50 PG (Lipoid, Ludwigshafen, German) were used in this study. .. Lentivirus-mediated gene transductionShort hairpin RNAs (shRNAs) targeting human 4E-BP1, Bax, Bak and Mcl-1, and a scrambled (control) shRNA were inserted into the lentiviral vector pLKD-CMV-GFP-U6-shRNA.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Jena Bioscience immobilized 2 3 eda m7gtp
    Immobilized 2 3 Eda M7gtp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immobilized 2 3 eda m7gtp/product/Jena Bioscience
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immobilized 2 3 eda m7gtp - by Bioz Stars, 2021-06
    91/100 stars
      Buy from Supplier

    92
    Jena Bioscience 2 3 eda m7 gtp
    2 3 Eda M7 Gtp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 3 eda m7 gtp/product/Jena Bioscience
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 3 eda m7 gtp - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

    Image Search Results