abi steponeplus  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Thermo Fisher abi steponeplus
    Abi Steponeplus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus/product/Thermo Fisher
    Average 97 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus - by Bioz Stars, 2020-04
    97/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Targeting the PI3K/AKT pathway via GLI1 inhibition enhanced the drug sensitivity of acute myeloid leukemia cells
    Article Snippet: .. Total RNA (500 ng) was reverse transcribed into first strand cDNA using a PrimeScriptTM RT Reagent Kit (TaKaRa, Japan, Cat#RR047A). cDNA was amplified in a total volume of 10 μl using SYBR qPCR Mix (Toy-obo, TOYOBO, Japan, Lot:266400), and the amplification was performed by ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) with specific primers ( ). .. Western blot analysis Equal amounts of protein were solubilized in sample buffer and electrophoresed on denaturing SDS-polyacrylamide gels and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA).

    Article Title: High resolution analysis of rare copy number variants in patients with autism spectrum disorder from Taiwan
    Article Snippet: The experiment was implemented using the ABI StepOnePlus following the manufacturer’s protocol (Applied Biosystems, Forster City, California, USA). .. The description of primer sequences, optimal annealing temperature, and the amplicon sizes is available upon request.

    Synthesized:

    Article Title: HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway
    Article Snippet: RNA concentration and purity were measured using Nanodrop 2000, and the overconcentrated RNA was diluted in an appropriate ratio to a final concentration of 200 ng/μ L. cDNA was synthesized from 2 μ g of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo, USA). .. The reaction conditions were as follows: the predenaturation was 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 60 s performed with ABI StepOnePlus (Applied Biosystems).

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: .. RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). ..

    Article Title: Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells
    Article Snippet: In short, the total cellular RNA was extracted from the three groups (24, 48 and 72 h post-transfection) with a miRCURY RNA Isolation Kit™ (Exiqon) and concentration of total RNA was measured by 260/280 absorbance, then cDNA was synthesized using the universal cDNA synthesis Kit™ (Exiqon). .. ABI_StepOnePlus (Applied Biosystems, Foster City, CA, USA) instrument was used for real-time PCR experiments and the ΔΔCt method was chosen for data calculation.

    Quantitative RT-PCR:

    Article Title: Targeting the PI3K/AKT pathway via GLI1 inhibition enhanced the drug sensitivity of acute myeloid leukemia cells
    Article Snippet: Paragraph title: Quantitative RT-PCR and RT-PCR ... Total RNA (500 ng) was reverse transcribed into first strand cDNA using a PrimeScriptTM RT Reagent Kit (TaKaRa, Japan, Cat#RR047A). cDNA was amplified in a total volume of 10 μl using SYBR qPCR Mix (Toy-obo, TOYOBO, Japan, Lot:266400), and the amplification was performed by ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) with specific primers ( ).

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: .. RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). ..

    Article Title: Long noncoding RNA LINC01296 is associated with poor prognosis in prostate cancer and promotes cancer-cell proliferation and metastasis
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) was performed on an ABI StepOnePlus (Thermo Fisher Scientific) using a standard protocol from Power SYBR Green (Takara) according to the manufacturer’s instructions; the reaction was performed in 10 μL reaction volume containing 5 μL of SYBR Premix (2×), 0.4 μL of PCR forward primer (10 μM), 0.4 μL of PCR reverse primer (10 μM), 0.2 μL of ROX Reference Dye II (50×), 3 μL of double-distilled water, and 1 μL of complementary DNA of the sample. qRT-PCR was performed under the following conditions: 95°C for 10 minutes, 95°C for 30 seconds, 95°C for 5 seconds, 61°C for 30 seconds in cycles for a total of 50 cycles, and a final extension step at 72°C for 5 minutes. ..

    Article Title: Neutralization of CD40 ligand costimulation promotes bone formation and accretion of vertebral bone mass in mice
    Article Snippet: .. Real-time RT-PCR was performed on an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) as previously described [ ] using commercial (Applied Biosystems, Foster City, CA, USA) master mix, and primer sets and probes for murine Wnt-10b (Mm00442104 ) and 18 S ribosomal RNA (Mm03928990). ..

    Article Title: High resolution analysis of rare copy number variants in patients with autism spectrum disorder from Taiwan
    Article Snippet: Paragraph title: Real-time quantitative PCR (RT-qPCR) ... The experiment was implemented using the ABI StepOnePlus following the manufacturer’s protocol (Applied Biosystems, Forster City, California, USA).

    Article Title: A novel real-time RT-PCR assay for influenza C tested in Peruvian children
    Article Snippet: .. Real-time RT-PCR was performed in 25-µL reactions containing 0.8 µM forward and reverse primers, 0.2 µM probe, and 5 µL of extracted RNA on an ABI StepOnePlus (Thermo Fisher) using the AgPath-ID One-Step RT-PCR kit (Thermo Fisher). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Targeting the PI3K/AKT pathway via GLI1 inhibition enhanced the drug sensitivity of acute myeloid leukemia cells
    Article Snippet: .. Total RNA (500 ng) was reverse transcribed into first strand cDNA using a PrimeScriptTM RT Reagent Kit (TaKaRa, Japan, Cat#RR047A). cDNA was amplified in a total volume of 10 μl using SYBR qPCR Mix (Toy-obo, TOYOBO, Japan, Lot:266400), and the amplification was performed by ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) with specific primers ( ). .. Western blot analysis Equal amounts of protein were solubilized in sample buffer and electrophoresed on denaturing SDS-polyacrylamide gels and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA).

    Article Title: How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments
    Article Snippet: One serial dilution was used to generate 6 standard samples that each was measured in qPCR tetraplicates on 6 different plates (using recommended plastic for each instrument) on 6 different instruments. .. The instruments were: Bio-Rad CFX 96 (Bio-Rad), ABI 7500 Fast (Applied Biosystems), ABI StepOnePlus (Life Technologies, Applied Biosystems), RotorGene 6000 duplex (Qiagen), Realplex4 gradient S Mastercycler (Eppendorf), and 384 well plate platform LightCycler 480 (Roche Diagnostics).

    Article Title: The PI3K/Akt signal hyperactivates Eya1 via the SUMOylation pathway
    Article Snippet: .. Real-time quantitative PCR Real-time quantitative PCR was performed using VeriQuest™ SYBR® Green (Affimetrix) on an ABI StepOnePlus (Applied Biosystems). .. PCR primers were 5’-ACT TAC CAG CTT CAA GAA CCG-3' and 5'-ATT TCC CAT CTG AAC CTC GAC-3' for EYA1 ; 5’-CAC AGC CCA CTG GTC CTC AAG AGG-3’ and 5’- TGT TTC AAC TGT TCT CGT CGT TTC C-3’ for c-MYC ; 5’- GCC CTC TGT GCC ACA GAT GTG AAG-3’ and 5’-GTT CTG CTG GGC CTG GCG CAG GCT-3’ for CYCLIN D1 ; 5’-CGC TGA GCT GCC TGC TGT TGC-3’ and 5’-GGT AGG TCC GGA TGA GGC GGC-3’ for FGF8 ; 5’-GCC TCA AGA TCA TCA GCA AT-3’ and 5’-TTC AGC TCA GGG ATG ACC TT-3’ for GAPDH .

    Article Title: HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway
    Article Snippet: Paragraph title: 2.11. Real-Time PCR ... The reaction conditions were as follows: the predenaturation was 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 60 s performed with ABI StepOnePlus (Applied Biosystems).

    Article Title: Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells
    Article Snippet: .. ABI_StepOnePlus (Applied Biosystems, Foster City, CA, USA) instrument was used for real-time PCR experiments and the ΔΔCt method was chosen for data calculation. .. To assess the cell viability, MTT assay was used that is based on the reduction of MTT (3-[4,5dimethylthiazol-2-yl]-2,5- diphenyl tetrazolium bromide) by the mitochondrial dehydrogenases of living cells to a purple formazan product.

    Article Title: Long noncoding RNA LINC01296 is associated with poor prognosis in prostate cancer and promotes cancer-cell proliferation and metastasis
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) was performed on an ABI StepOnePlus (Thermo Fisher Scientific) using a standard protocol from Power SYBR Green (Takara) according to the manufacturer’s instructions; the reaction was performed in 10 μL reaction volume containing 5 μL of SYBR Premix (2×), 0.4 μL of PCR forward primer (10 μM), 0.4 μL of PCR reverse primer (10 μM), 0.2 μL of ROX Reference Dye II (50×), 3 μL of double-distilled water, and 1 μL of complementary DNA of the sample. qRT-PCR was performed under the following conditions: 95°C for 10 minutes, 95°C for 30 seconds, 95°C for 5 seconds, 61°C for 30 seconds in cycles for a total of 50 cycles, and a final extension step at 72°C for 5 minutes. ..

    Article Title: High resolution analysis of rare copy number variants in patients with autism spectrum disorder from Taiwan
    Article Snippet: Paragraph title: Real-time quantitative PCR (RT-qPCR) ... The experiment was implemented using the ABI StepOnePlus following the manufacturer’s protocol (Applied Biosystems, Forster City, California, USA).

    Article Title: The schistosome glutathione S-transferase P28GST, a unique helminth protein, prevents intestinal inflammation in experimental colitis through a Th2-type response with mucosal eosinophils
    Article Snippet: Real-time quantitative PCR was carried out with the Fast Power SYBR Green PCR master mix (Life Technologies) according to the manufacturer's instructions. .. The analyses were performed with an ABI StepOnePlus (Applied Biosystems, Life Technologies).

    Expressing:

    Article Title: Epidermal Growth Factor Attenuated the Expression of Inflammatory Cytokines in Human Epidermal Keratinocyte Exposed to Propionibacterium acnes
    Article Snippet: Probes were obtained from Applied Biosystems (Foster City, CA, USA) as Assays-on-Demand™ Gene Expression Assays (glyceraldehyde-3-phosphate dehydrogenase [GAPDH]: Hs02758991_g1, IL-1α: Hs00174092_m1, IL-8: Hs00174103_m1, TNF-α: Hs01113624_m1, TLR2: Hs01872448_s1, NF-κB: Hs00 765730_m1, p38α: Hs01051152_m1). .. Reactions were carried out on the ABI StepOnePlus™ (Applied Biosystems), and relative transcription levels were determined by GAPDH as the reference gene.

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). .. The PCR conditions were as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s. Data were normalized to the expression of GAPDH, and PCR products were separated on a 1.5% agarose gel containing ethidium bromide, for quantification by densitometry using Image J software (National Institutes of Health, USA).

    miRNA RT:

    Article Title: Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells
    Article Snippet: Paragraph title: Reverse transcriptase (RT) miRNA real-time PCR ... ABI_StepOnePlus (Applied Biosystems, Foster City, CA, USA) instrument was used for real-time PCR experiments and the ΔΔCt method was chosen for data calculation.

    Over Expression:

    Article Title: Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells
    Article Snippet: Reverse transcriptase (RT) miRNA real-time PCR was performed to evaluate the efficiency of hsa-let-7c-5p overexpression by the hsa-let-7c-5p miRNA mimic. .. ABI_StepOnePlus (Applied Biosystems, Foster City, CA, USA) instrument was used for real-time PCR experiments and the ΔΔCt method was chosen for data calculation.

    Serial Dilution:

    Article Title: How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments
    Article Snippet: One serial dilution was used to generate 6 standard samples that each was measured in qPCR tetraplicates on 6 different plates (using recommended plastic for each instrument) on 6 different instruments. .. The instruments were: Bio-Rad CFX 96 (Bio-Rad), ABI 7500 Fast (Applied Biosystems), ABI StepOnePlus (Life Technologies, Applied Biosystems), RotorGene 6000 duplex (Qiagen), Realplex4 gradient S Mastercycler (Eppendorf), and 384 well plate platform LightCycler 480 (Roche Diagnostics).

    Cell Culture:

    Article Title: Long noncoding RNA LINC01296 is associated with poor prognosis in prostate cancer and promotes cancer-cell proliferation and metastasis
    Article Snippet: RNA isolation and quantitative real-time polymerase chain reaction Total RNA was extracted from tissues or cultured cell lines using Trizol reagent (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. Quantitative real-time PCR (qRT-PCR) was performed on an ABI StepOnePlus (Thermo Fisher Scientific) using a standard protocol from Power SYBR Green (Takara) according to the manufacturer’s instructions; the reaction was performed in 10 μL reaction volume containing 5 μL of SYBR Premix (2×), 0.4 μL of PCR forward primer (10 μM), 0.4 μL of PCR reverse primer (10 μM), 0.2 μL of ROX Reference Dye II (50×), 3 μL of double-distilled water, and 1 μL of complementary DNA of the sample. qRT-PCR was performed under the following conditions: 95°C for 10 minutes, 95°C for 30 seconds, 95°C for 5 seconds, 61°C for 30 seconds in cycles for a total of 50 cycles, and a final extension step at 72°C for 5 minutes.

    Generated:

    Article Title: The schistosome glutathione S-transferase P28GST, a unique helminth protein, prevents intestinal inflammation in experimental colitis through a Th2-type response with mucosal eosinophils
    Article Snippet: RNA was extracted from colon samples with NucleoSpin RNA II kit (Macherey-Nagel, Hoerdt, France). cDNA was generated from 1 μg total RNA using a High Capacity cDNA Reverse Transcription Kit (Life Technologies, Saint-Aubin, France). .. The analyses were performed with an ABI StepOnePlus (Applied Biosystems, Life Technologies).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Targeting the PI3K/AKT pathway via GLI1 inhibition enhanced the drug sensitivity of acute myeloid leukemia cells
    Article Snippet: Paragraph title: Quantitative RT-PCR and RT-PCR ... Total RNA (500 ng) was reverse transcribed into first strand cDNA using a PrimeScriptTM RT Reagent Kit (TaKaRa, Japan, Cat#RR047A). cDNA was amplified in a total volume of 10 μl using SYBR qPCR Mix (Toy-obo, TOYOBO, Japan, Lot:266400), and the amplification was performed by ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) with specific primers ( ).

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: .. RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). ..

    Article Title: Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells
    Article Snippet: Synthetic RNA spike-in templates and its primers (Exiqon) were used as internal controls of RT-PCR. .. ABI_StepOnePlus (Applied Biosystems, Foster City, CA, USA) instrument was used for real-time PCR experiments and the ΔΔCt method was chosen for data calculation.

    Article Title: Long noncoding RNA LINC01296 is associated with poor prognosis in prostate cancer and promotes cancer-cell proliferation and metastasis
    Article Snippet: For reverse-transcription polymerase chain reaction (PCR), 1 μg of total RNA was reverse-transcribed to complementary DNA in a final volume of 10 μL by using random primers (10 μM) and a reverse-transcription kit (Takara, Kyoto, Japan). .. Quantitative real-time PCR (qRT-PCR) was performed on an ABI StepOnePlus (Thermo Fisher Scientific) using a standard protocol from Power SYBR Green (Takara) according to the manufacturer’s instructions; the reaction was performed in 10 μL reaction volume containing 5 μL of SYBR Premix (2×), 0.4 μL of PCR forward primer (10 μM), 0.4 μL of PCR reverse primer (10 μM), 0.2 μL of ROX Reference Dye II (50×), 3 μL of double-distilled water, and 1 μL of complementary DNA of the sample. qRT-PCR was performed under the following conditions: 95°C for 10 minutes, 95°C for 30 seconds, 95°C for 5 seconds, 61°C for 30 seconds in cycles for a total of 50 cycles, and a final extension step at 72°C for 5 minutes.

    Article Title: A novel real-time RT-PCR assay for influenza C tested in Peruvian children
    Article Snippet: .. Real-time RT-PCR was performed in 25-µL reactions containing 0.8 µM forward and reverse primers, 0.2 µM probe, and 5 µL of extracted RNA on an ABI StepOnePlus (Thermo Fisher) using the AgPath-ID One-Step RT-PCR kit (Thermo Fisher). ..

    Cellular Antioxidant Activity Assay:

    Article Title: The PI3K/Akt signal hyperactivates Eya1 via the SUMOylation pathway
    Article Snippet: Real-time quantitative PCR Real-time quantitative PCR was performed using VeriQuest™ SYBR® Green (Affimetrix) on an ABI StepOnePlus (Applied Biosystems). .. PCR primers were 5’-ACT TAC CAG CTT CAA GAA CCG-3' and 5'-ATT TCC CAT CTG AAC CTC GAC-3' for EYA1 ; 5’-CAC AGC CCA CTG GTC CTC AAG AGG-3’ and 5’- TGT TTC AAC TGT TCT CGT CGT TTC C-3’ for c-MYC ; 5’- GCC CTC TGT GCC ACA GAT GTG AAG-3’ and 5’-GTT CTG CTG GGC CTG GCG CAG GCT-3’ for CYCLIN D1 ; 5’-CGC TGA GCT GCC TGC TGT TGC-3’ and 5’-GGT AGG TCC GGA TGA GGC GGC-3’ for FGF8 ; 5’-GCC TCA AGA TCA TCA GCA AT-3’ and 5’-TTC AGC TCA GGG ATG ACC TT-3’ for GAPDH .

    Fluorescence:

    Article Title: A novel real-time RT-PCR assay for influenza C tested in Peruvian children
    Article Snippet: Real-time RT-PCR was performed in 25-µL reactions containing 0.8 µM forward and reverse primers, 0.2 µM probe, and 5 µL of extracted RNA on an ABI StepOnePlus (Thermo Fisher) using the AgPath-ID One-Step RT-PCR kit (Thermo Fisher). .. Fluorescence was measured during the 55°C annealing/extension step.

    Isolation:

    Article Title: Targeting the PI3K/AKT pathway via GLI1 inhibition enhanced the drug sensitivity of acute myeloid leukemia cells
    Article Snippet: Quantitative RT-PCR and RT-PCR RNA was isolated from 1 × 106 cells using TRIzol reagent (TaKaRa, Japan, Cat#9109). .. Total RNA (500 ng) was reverse transcribed into first strand cDNA using a PrimeScriptTM RT Reagent Kit (TaKaRa, Japan, Cat#RR047A). cDNA was amplified in a total volume of 10 μl using SYBR qPCR Mix (Toy-obo, TOYOBO, Japan, Lot:266400), and the amplification was performed by ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) with specific primers ( ).

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: .. RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). ..

    Article Title: Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells
    Article Snippet: In short, the total cellular RNA was extracted from the three groups (24, 48 and 72 h post-transfection) with a miRCURY RNA Isolation Kit™ (Exiqon) and concentration of total RNA was measured by 260/280 absorbance, then cDNA was synthesized using the universal cDNA synthesis Kit™ (Exiqon). .. ABI_StepOnePlus (Applied Biosystems, Foster City, CA, USA) instrument was used for real-time PCR experiments and the ΔΔCt method was chosen for data calculation.

    Article Title: Long noncoding RNA LINC01296 is associated with poor prognosis in prostate cancer and promotes cancer-cell proliferation and metastasis
    Article Snippet: Paragraph title: RNA isolation and quantitative real-time polymerase chain reaction ... Quantitative real-time PCR (qRT-PCR) was performed on an ABI StepOnePlus (Thermo Fisher Scientific) using a standard protocol from Power SYBR Green (Takara) according to the manufacturer’s instructions; the reaction was performed in 10 μL reaction volume containing 5 μL of SYBR Premix (2×), 0.4 μL of PCR forward primer (10 μM), 0.4 μL of PCR reverse primer (10 μM), 0.2 μL of ROX Reference Dye II (50×), 3 μL of double-distilled water, and 1 μL of complementary DNA of the sample. qRT-PCR was performed under the following conditions: 95°C for 10 minutes, 95°C for 30 seconds, 95°C for 5 seconds, 61°C for 30 seconds in cycles for a total of 50 cycles, and a final extension step at 72°C for 5 minutes.

    Polymerase Chain Reaction:

    Article Title: The PI3K/Akt signal hyperactivates Eya1 via the SUMOylation pathway
    Article Snippet: Real-time quantitative PCR Real-time quantitative PCR was performed using VeriQuest™ SYBR® Green (Affimetrix) on an ABI StepOnePlus (Applied Biosystems). .. PCR primers were 5’-ACT TAC CAG CTT CAA GAA CCG-3' and 5'-ATT TCC CAT CTG AAC CTC GAC-3' for EYA1 ; 5’-CAC AGC CCA CTG GTC CTC AAG AGG-3’ and 5’- TGT TTC AAC TGT TCT CGT CGT TTC C-3’ for c-MYC ; 5’- GCC CTC TGT GCC ACA GAT GTG AAG-3’ and 5’-GTT CTG CTG GGC CTG GCG CAG GCT-3’ for CYCLIN D1 ; 5’-CGC TGA GCT GCC TGC TGT TGC-3’ and 5’-GGT AGG TCC GGA TGA GGC GGC-3’ for FGF8 ; 5’-GCC TCA AGA TCA TCA GCA AT-3’ and 5’-TTC AGC TCA GGG ATG ACC TT-3’ for GAPDH .

    Article Title: Epidermal Growth Factor Attenuated the Expression of Inflammatory Cytokines in Human Epidermal Keratinocyte Exposed to Propionibacterium acnes
    Article Snippet: Paragraph title: Real-time reverse-transcription–quantitative polymerase chain reaction ... Reactions were carried out on the ABI StepOnePlus™ (Applied Biosystems), and relative transcription levels were determined by GAPDH as the reference gene.

    Article Title: HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway
    Article Snippet: For real-time PCR, the 0.2 mL PCR tube was prepared to prepare the following reaction system, and all reactions were performed in triplicate: 2× SYBR qPCR Master Mix (Roche, Switzerland) (12.5 μ L), 7.5 μ M gene primer (2.0 μ L), reverse primer (2.5 μ L), and ddH2O (8.0 μ L). .. The reaction conditions were as follows: the predenaturation was 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 60 s performed with ABI StepOnePlus (Applied Biosystems).

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). .. The PCR conditions were as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s. Data were normalized to the expression of GAPDH, and PCR products were separated on a 1.5% agarose gel containing ethidium bromide, for quantification by densitometry using Image J software (National Institutes of Health, USA).

    Article Title: Long noncoding RNA LINC01296 is associated with poor prognosis in prostate cancer and promotes cancer-cell proliferation and metastasis
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) was performed on an ABI StepOnePlus (Thermo Fisher Scientific) using a standard protocol from Power SYBR Green (Takara) according to the manufacturer’s instructions; the reaction was performed in 10 μL reaction volume containing 5 μL of SYBR Premix (2×), 0.4 μL of PCR forward primer (10 μM), 0.4 μL of PCR reverse primer (10 μM), 0.2 μL of ROX Reference Dye II (50×), 3 μL of double-distilled water, and 1 μL of complementary DNA of the sample. qRT-PCR was performed under the following conditions: 95°C for 10 minutes, 95°C for 30 seconds, 95°C for 5 seconds, 61°C for 30 seconds in cycles for a total of 50 cycles, and a final extension step at 72°C for 5 minutes. ..

    Article Title: High resolution analysis of rare copy number variants in patients with autism spectrum disorder from Taiwan
    Article Snippet: RT-qPCR was performed using the SYBR-Green PCR reagents kit (Applied Biosystems, Forster City, California, USA), and the CNV was assessed using a relatively standard method in the laboratory . .. The experiment was implemented using the ABI StepOnePlus following the manufacturer’s protocol (Applied Biosystems, Forster City, California, USA).

    Article Title: The schistosome glutathione S-transferase P28GST, a unique helminth protein, prevents intestinal inflammation in experimental colitis through a Th2-type response with mucosal eosinophils
    Article Snippet: Real-time quantitative PCR was carried out with the Fast Power SYBR Green PCR master mix (Life Technologies) according to the manufacturer's instructions. .. The analyses were performed with an ABI StepOnePlus (Applied Biosystems, Life Technologies).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Targeting the PI3K/AKT pathway via GLI1 inhibition enhanced the drug sensitivity of acute myeloid leukemia cells
    Article Snippet: .. Total RNA (500 ng) was reverse transcribed into first strand cDNA using a PrimeScriptTM RT Reagent Kit (TaKaRa, Japan, CatRR047A). cDNA was amplified in a total volume of 10 μl using SYBR qPCR Mix (Toy-obo, TOYOBO, Japan, Lot:266400), and the amplification was performed by ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) with specific primers ( ). .. Western blot analysis Equal amounts of protein were solubilized in sample buffer and electrophoresed on denaturing SDS-polyacrylamide gels and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA).

    Article Title: The PI3K/Akt signal hyperactivates Eya1 via the SUMOylation pathway
    Article Snippet: Real-time quantitative PCR Real-time quantitative PCR was performed using VeriQuest™ SYBR® Green (Affimetrix) on an ABI StepOnePlus (Applied Biosystems). .. PCR primers were 5’-ACT TAC CAG CTT CAA GAA CCG-3' and 5'-ATT TCC CAT CTG AAC CTC GAC-3' for EYA1 ; 5’-CAC AGC CCA CTG GTC CTC AAG AGG-3’ and 5’- TGT TTC AAC TGT TCT CGT CGT TTC C-3’ for c-MYC ; 5’- GCC CTC TGT GCC ACA GAT GTG AAG-3’ and 5’-GTT CTG CTG GGC CTG GCG CAG GCT-3’ for CYCLIN D1 ; 5’-CGC TGA GCT GCC TGC TGT TGC-3’ and 5’-GGT AGG TCC GGA TGA GGC GGC-3’ for FGF8 ; 5’-GCC TCA AGA TCA TCA GCA AT-3’ and 5’-TTC AGC TCA GGG ATG ACC TT-3’ for GAPDH .

    Software:

    Article Title: Epidermal Growth Factor Attenuated the Expression of Inflammatory Cytokines in Human Epidermal Keratinocyte Exposed to Propionibacterium acnes
    Article Snippet: Reactions were carried out on the ABI StepOnePlus™ (Applied Biosystems), and relative transcription levels were determined by GAPDH as the reference gene. .. The data were analysed by using the ABI StepOnePlus™ software (Applied Biosystems).

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). .. The PCR conditions were as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s. Data were normalized to the expression of GAPDH, and PCR products were separated on a 1.5% agarose gel containing ethidium bromide, for quantification by densitometry using Image J software (National Institutes of Health, USA).

    SYBR Green Assay:

    Article Title: The PI3K/Akt signal hyperactivates Eya1 via the SUMOylation pathway
    Article Snippet: .. Real-time quantitative PCR Real-time quantitative PCR was performed using VeriQuest™ SYBR® Green (Affimetrix) on an ABI StepOnePlus (Applied Biosystems). .. PCR primers were 5’-ACT TAC CAG CTT CAA GAA CCG-3' and 5'-ATT TCC CAT CTG AAC CTC GAC-3' for EYA1 ; 5’-CAC AGC CCA CTG GTC CTC AAG AGG-3’ and 5’- TGT TTC AAC TGT TCT CGT CGT TTC C-3’ for c-MYC ; 5’- GCC CTC TGT GCC ACA GAT GTG AAG-3’ and 5’-GTT CTG CTG GGC CTG GCG CAG GCT-3’ for CYCLIN D1 ; 5’-CGC TGA GCT GCC TGC TGT TGC-3’ and 5’-GGT AGG TCC GGA TGA GGC GGC-3’ for FGF8 ; 5’-GCC TCA AGA TCA TCA GCA AT-3’ and 5’-TTC AGC TCA GGG ATG ACC TT-3’ for GAPDH .

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: .. RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). ..

    Article Title: Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells
    Article Snippet: Real-time PCR was performed by using the SYBR® Green master mix Kit™ (Exiqon) and specific hsa-let-7c-5p primers (Exiqon). .. ABI_StepOnePlus (Applied Biosystems, Foster City, CA, USA) instrument was used for real-time PCR experiments and the ΔΔCt method was chosen for data calculation.

    Article Title: Long noncoding RNA LINC01296 is associated with poor prognosis in prostate cancer and promotes cancer-cell proliferation and metastasis
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) was performed on an ABI StepOnePlus (Thermo Fisher Scientific) using a standard protocol from Power SYBR Green (Takara) according to the manufacturer’s instructions; the reaction was performed in 10 μL reaction volume containing 5 μL of SYBR Premix (2×), 0.4 μL of PCR forward primer (10 μM), 0.4 μL of PCR reverse primer (10 μM), 0.2 μL of ROX Reference Dye II (50×), 3 μL of double-distilled water, and 1 μL of complementary DNA of the sample. qRT-PCR was performed under the following conditions: 95°C for 10 minutes, 95°C for 30 seconds, 95°C for 5 seconds, 61°C for 30 seconds in cycles for a total of 50 cycles, and a final extension step at 72°C for 5 minutes. ..

    Article Title: High resolution analysis of rare copy number variants in patients with autism spectrum disorder from Taiwan
    Article Snippet: RT-qPCR was performed using the SYBR-Green PCR reagents kit (Applied Biosystems, Forster City, California, USA), and the CNV was assessed using a relatively standard method in the laboratory . .. The experiment was implemented using the ABI StepOnePlus following the manufacturer’s protocol (Applied Biosystems, Forster City, California, USA).

    Article Title: The schistosome glutathione S-transferase P28GST, a unique helminth protein, prevents intestinal inflammation in experimental colitis through a Th2-type response with mucosal eosinophils
    Article Snippet: Real-time quantitative PCR was carried out with the Fast Power SYBR Green PCR master mix (Life Technologies) according to the manufacturer's instructions. .. The analyses were performed with an ABI StepOnePlus (Applied Biosystems, Life Technologies).

    Negative Control:

    Article Title: A novel real-time RT-PCR assay for influenza C tested in Peruvian children
    Article Snippet: Real-time RT-PCR was performed in 25-µL reactions containing 0.8 µM forward and reverse primers, 0.2 µM probe, and 5 µL of extracted RNA on an ABI StepOnePlus (Thermo Fisher) using the AgPath-ID One-Step RT-PCR kit (Thermo Fisher). .. Nuclease-free water served as negative control in all experiments.

    Agarose Gel Electrophoresis:

    Article Title: Pathophysiology of Endometriosis: Role of High Mobility Group Box-1 and Toll-Like Receptor 4 Developing Inflammation in Endometrium
    Article Snippet: RNA isolation and qRT-PCR Cell-free total RNA was extracted from cell lysates using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg total RNA using oligo-dT primers (Invitrogen). qRT-PCR for TLR4 was carried out using an ABI StepOnePlus (Applied Biosystems, Foster City, CA, USA) and SYBR green RT PCR master mix (Toyobo, Osaka, Japan). .. The PCR conditions were as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for 1 min, and 95°C for 15 s. Data were normalized to the expression of GAPDH, and PCR products were separated on a 1.5% agarose gel containing ethidium bromide, for quantification by densitometry using Image J software (National Institutes of Health, USA).

    Concentration Assay:

    Article Title: HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway
    Article Snippet: RNA concentration and purity were measured using Nanodrop 2000, and the overconcentrated RNA was diluted in an appropriate ratio to a final concentration of 200 ng/μ L. cDNA was synthesized from 2 μ g of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo, USA). .. The reaction conditions were as follows: the predenaturation was 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 60 s performed with ABI StepOnePlus (Applied Biosystems).

    Article Title: Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells
    Article Snippet: In short, the total cellular RNA was extracted from the three groups (24, 48 and 72 h post-transfection) with a miRCURY RNA Isolation Kit™ (Exiqon) and concentration of total RNA was measured by 260/280 absorbance, then cDNA was synthesized using the universal cDNA synthesis Kit™ (Exiqon). .. ABI_StepOnePlus (Applied Biosystems, Foster City, CA, USA) instrument was used for real-time PCR experiments and the ΔΔCt method was chosen for data calculation.

    CTG Assay:

    Article Title: The PI3K/Akt signal hyperactivates Eya1 via the SUMOylation pathway
    Article Snippet: Real-time quantitative PCR Real-time quantitative PCR was performed using VeriQuest™ SYBR® Green (Affimetrix) on an ABI StepOnePlus (Applied Biosystems). .. PCR primers were 5’-ACT TAC CAG CTT CAA GAA CCG-3' and 5'-ATT TCC CAT CTG AAC CTC GAC-3' for EYA1 ; 5’-CAC AGC CCA CTG GTC CTC AAG AGG-3’ and 5’- TGT TTC AAC TGT TCT CGT CGT TTC C-3’ for c-MYC ; 5’- GCC CTC TGT GCC ACA GAT GTG AAG-3’ and 5’-GTT CTG CTG GGC CTG GCG CAG GCT-3’ for CYCLIN D1 ; 5’-CGC TGA GCT GCC TGC TGT TGC-3’ and 5’-GGT AGG TCC GGA TGA GGC GGC-3’ for FGF8 ; 5’-GCC TCA AGA TCA TCA GCA AT-3’ and 5’-TTC AGC TCA GGG ATG ACC TT-3’ for GAPDH .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher abi steponeplus real time pcr machine
    Abi Steponeplus Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus real time pcr machine/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus real time pcr machine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher abi steponeplus thermocycler
    Abi Steponeplus Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus thermocycler/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus thermocycler - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    97
    Thermo Fisher abi steponeplus
    Abi Steponeplus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus/product/Thermo Fisher
    Average 97 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    94
    Thermo Fisher abi steponeplus system
    Abi Steponeplus System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus system/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus system - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results