abi steponeplus thermocycler  (Thermo Fisher)


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    Structured Review

    Thermo Fisher abi steponeplus thermocycler
    Abi Steponeplus Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus thermocycler/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus thermocycler - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Transduction:

    Article Title: MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-κB
    Article Snippet: Quantitative real-time-PCR (qRT-PCR) Six days after transduction, total RNA was extracted by using the RNeasy Mini kit (Qiagen) in accordance with the manufacturer’s instructions. cDNA was synthesized by using oligo(dT) primers and RevertAid H Minus M-MuLV Reverse Transcriptase (Thermo Scientific) according to the manufacturer’s instructions. .. All quantitative RT-PCR reactions were performed in triplicates by an ABI StepOnePlus thermocycler (Applied Biosystems) with SYBR Green PCR Master Mix (Thermo Scientific).

    Amplification:

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems). .. After amplification, data were analyzed and exported by Sequence Detection Software (SDS) for the value of the threshold cycle (C t ), differences in C t values (ΔC t ) between the test locus and the control locus (GAPDH), and the comparative C t (ΔΔC t ) were used to calculate patient sample copy number value compared with the volunteer sample by relative quantitation (fold change).

    Article Title: PD-1 Dynamically Regulates Inflammation and Development of Brain-Resident Memory CD8 T Cells During Persistent Viral Encephalitis
    Article Snippet: Quantification of MuPyV Genomes and LT-Ag mRNA TaqMan real-time PCR was performed in an ABI StepOnePlus thermocycler (Applied Biosciences) with 10 μg of template DNA purified from tissues using the Maxwell 16 Research Instrument (Promega, Madison, WI) according to the manufacturer's instructions. .. Primers and amplification parameters are previously described ( ).

    Article Title: ACTG1 and TLR3 are biomarkers for alcohol-associated hepatocellular carcinoma
    Article Snippet: RNA (1 µg) was reverse transcribed into cDNA using FastQuant RT kit (with gDNase) (Tiangen Biotech Co., Ltd., Beijing, China), according to the manufacturers protocol. cDNA was amplified using SYBR Green Real Master mix (Tiangen Biotech Co., Ltd.), according to the manufacturers protocol. .. The PCR reaction was conducted at 95°C for 15 min, followed by 40 cycles at 95°C for 10 sec, 58°C for 20 sec and 72°C for 30 sec. RT-qPCR was performed on the ABI StepOnePlus™ thermocycler (Thermo Fisher Scientific, Inc.).

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: .. Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems). .. The amplification protocol was according to manufacturer's recommendation and included an initial denaturing step at 90°C for 10min, followed by 45 cycles with denaturation at 95°C for 15 sec and an annealing/elongation step of 60°C for 1min.

    Synthesized:

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: PCR was performed in triplicate in a 20-μl reaction volume containing 10 μl Go-Taq SYBR-Green PCR Master Mix (Applied Biosystems, Beijing, China), 10 µmol forward and reverse primers ( ; synthesized by Beijing Genomics Institute, Beijing, China), and 10 ng of gDNA. .. The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems).

    Article Title: MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-κB
    Article Snippet: Quantitative real-time-PCR (qRT-PCR) Six days after transduction, total RNA was extracted by using the RNeasy Mini kit (Qiagen) in accordance with the manufacturer’s instructions. cDNA was synthesized by using oligo(dT) primers and RevertAid H Minus M-MuLV Reverse Transcriptase (Thermo Scientific) according to the manufacturer’s instructions. .. All quantitative RT-PCR reactions were performed in triplicates by an ABI StepOnePlus thermocycler (Applied Biosystems) with SYBR Green PCR Master Mix (Thermo Scientific).

    Article Title: Cabazitaxel-induced autophagy via the PI3K/Akt/mTOR pathway contributes to A549 cell death
    Article Snippet: Subsequently, cDNA was synthesized using the RevertAid First Strand cDNA synthesis kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA). .. Briefly, the total volume of 20 µ l reverse transcription mixer (500 ng RNA, 1 µ l oligo(dT)18, 1µ l RevertAid RT) was incubated for 60 min at 42°C, then the reaction was terminated by heating at 70°C for 5 min. qPCR was performed on an ABI StepOnePlus™ thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc., Foster City, CA, USA) using a SYBR® Green PCR kit (Applied Biosystems; Thermo Fisher Scientific, Inc.).

    Article Title: Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro
    Article Snippet: RNA isolation and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted from cells using the TRIzol reagent, and cDNA was synthesized from 1,000 ng of total RNA using a reverse transcription kit according to the manufacturer’s protocol. .. The qRT-PCR was performed using an ABI StepOnePlus thermocycler (Applied Biosystems) using the Fast SYBR Green Master Mix.

    Quantitative RT-PCR:

    Article Title: Differential protein‐coding gene and long noncoding RNA expression in smoking‐related lung squamous cell carcinoma
    Article Snippet: .. Total RNA was then reverse transcribed to cDNA using the Reverse Transcription Kit (Roche, Basel, Switzerland). qRT‐PCR reactions were performed using an ABI StepOnePlus Thermocycler using a SYBR Green PCR Kit (Thermo Fisher Scientific). ..

    Article Title: Identification of differentially expressed protein-coding genes in lung adenocarcinomas
    Article Snippet: To confirm the results of the present bioinformatics analysis the expression levels of TOP2A, CDC20, BUB1 and MAD2L1 in tumor tissues of two lung adenocarcinoma patients were quantified using RT-qPCR. .. A FastQuant RT kit (with gDNase; Tiangen Biotech Co., Ltd.) was used to reverse transcribe total RNA to cDNA; the temperature of the RT process was 42°C. qPCR was performed in an ABI StepOnePlus Thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc.) using a SYBR Green PCR kit (Qiagen GmbH).

    Article Title: MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-κB
    Article Snippet: .. All quantitative RT-PCR reactions were performed in triplicates by an ABI StepOnePlus thermocycler (Applied Biosystems) with SYBR Green PCR Master Mix (Thermo Scientific). ..

    Article Title: Cabazitaxel-induced autophagy via the PI3K/Akt/mTOR pathway contributes to A549 cell death
    Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... Briefly, the total volume of 20 µ l reverse transcription mixer (500 ng RNA, 1 µ l oligo(dT)18, 1µ l RevertAid RT) was incubated for 60 min at 42°C, then the reaction was terminated by heating at 70°C for 5 min. qPCR was performed on an ABI StepOnePlus™ thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc., Foster City, CA, USA) using a SYBR® Green PCR kit (Applied Biosystems; Thermo Fisher Scientific, Inc.).

    Article Title: ACTG1 and TLR3 are biomarkers for alcohol-associated hepatocellular carcinoma
    Article Snippet: .. The PCR reaction was conducted at 95°C for 15 min, followed by 40 cycles at 95°C for 10 sec, 58°C for 20 sec and 72°C for 30 sec. RT-qPCR was performed on the ABI StepOnePlus™ thermocycler (Thermo Fisher Scientific, Inc.). .. U6 single nuclear (sn)RNA was employed as the loading control for micro (mi)RNA expression, and β-actin for the expression of genes; stem-loop RT primers were used for miRNAs, and oligo (dinucleotide) primers for genes.

    Article Title: Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro
    Article Snippet: .. The qRT-PCR was performed using an ABI StepOnePlus thermocycler (Applied Biosystems) using the Fast SYBR Green Master Mix. .. Expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA box binding protein (TBP), and ribosomal protein, large, P0 (RPLP0) were used for normalization.

    SYBR Green Assay:

    Article Title: Differential protein‐coding gene and long noncoding RNA expression in smoking‐related lung squamous cell carcinoma
    Article Snippet: .. Total RNA was then reverse transcribed to cDNA using the Reverse Transcription Kit (Roche, Basel, Switzerland). qRT‐PCR reactions were performed using an ABI StepOnePlus Thermocycler using a SYBR Green PCR Kit (Thermo Fisher Scientific). ..

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: PCR was performed in triplicate in a 20-μl reaction volume containing 10 μl Go-Taq SYBR-Green PCR Master Mix (Applied Biosystems, Beijing, China), 10 µmol forward and reverse primers ( ; synthesized by Beijing Genomics Institute, Beijing, China), and 10 ng of gDNA. .. The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems).

    Article Title: Identification of differentially expressed protein-coding genes in lung adenocarcinomas
    Article Snippet: .. A FastQuant RT kit (with gDNase; Tiangen Biotech Co., Ltd.) was used to reverse transcribe total RNA to cDNA; the temperature of the RT process was 42°C. qPCR was performed in an ABI StepOnePlus Thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc.) using a SYBR Green PCR kit (Qiagen GmbH). ..

    Article Title: MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-κB
    Article Snippet: .. All quantitative RT-PCR reactions were performed in triplicates by an ABI StepOnePlus thermocycler (Applied Biosystems) with SYBR Green PCR Master Mix (Thermo Scientific). ..

    Article Title: Cabazitaxel-induced autophagy via the PI3K/Akt/mTOR pathway contributes to A549 cell death
    Article Snippet: .. Briefly, the total volume of 20 µ l reverse transcription mixer (500 ng RNA, 1 µ l oligo(dT)18, 1µ l RevertAid RT) was incubated for 60 min at 42°C, then the reaction was terminated by heating at 70°C for 5 min. qPCR was performed on an ABI StepOnePlus™ thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc., Foster City, CA, USA) using a SYBR® Green PCR kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. Each sample was run in triplicate in a final volume of 25 µ l containing 1 µ l first-strand cDNA template, 10 pmol of each primer ( ; obtained from Sangon Biotech Co., Ltd, Shanghai, China), 12.5 µ l of SYBR® Green PCR master mix and 11.5 µ l distilled water.

    Article Title: ACTG1 and TLR3 are biomarkers for alcohol-associated hepatocellular carcinoma
    Article Snippet: RNA (1 µg) was reverse transcribed into cDNA using FastQuant RT kit (with gDNase) (Tiangen Biotech Co., Ltd., Beijing, China), according to the manufacturers protocol. cDNA was amplified using SYBR Green Real Master mix (Tiangen Biotech Co., Ltd.), according to the manufacturers protocol. .. The PCR reaction was conducted at 95°C for 15 min, followed by 40 cycles at 95°C for 10 sec, 58°C for 20 sec and 72°C for 30 sec. RT-qPCR was performed on the ABI StepOnePlus™ thermocycler (Thermo Fisher Scientific, Inc.).

    Article Title: Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro
    Article Snippet: .. The qRT-PCR was performed using an ABI StepOnePlus thermocycler (Applied Biosystems) using the Fast SYBR Green Master Mix. .. Expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA box binding protein (TBP), and ribosomal protein, large, P0 (RPLP0) were used for normalization.

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: .. Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems). .. The amplification protocol was according to manufacturer's recommendation and included an initial denaturing step at 90°C for 10min, followed by 45 cycles with denaturation at 95°C for 15 sec and an annealing/elongation step of 60°C for 1min.

    Incubation:

    Article Title: Cabazitaxel-induced autophagy via the PI3K/Akt/mTOR pathway contributes to A549 cell death
    Article Snippet: .. Briefly, the total volume of 20 µ l reverse transcription mixer (500 ng RNA, 1 µ l oligo(dT)18, 1µ l RevertAid RT) was incubated for 60 min at 42°C, then the reaction was terminated by heating at 70°C for 5 min. qPCR was performed on an ABI StepOnePlus™ thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc., Foster City, CA, USA) using a SYBR® Green PCR kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. Each sample was run in triplicate in a final volume of 25 µ l containing 1 µ l first-strand cDNA template, 10 pmol of each primer ( ; obtained from Sangon Biotech Co., Ltd, Shanghai, China), 12.5 µ l of SYBR® Green PCR master mix and 11.5 µ l distilled water.

    Expressing:

    Article Title: Identification of differentially expressed protein-coding genes in lung adenocarcinomas
    Article Snippet: To confirm the results of the present bioinformatics analysis the expression levels of TOP2A, CDC20, BUB1 and MAD2L1 in tumor tissues of two lung adenocarcinoma patients were quantified using RT-qPCR. .. A FastQuant RT kit (with gDNase; Tiangen Biotech Co., Ltd.) was used to reverse transcribe total RNA to cDNA; the temperature of the RT process was 42°C. qPCR was performed in an ABI StepOnePlus Thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc.) using a SYBR Green PCR kit (Qiagen GmbH).

    Article Title: ACTG1 and TLR3 are biomarkers for alcohol-associated hepatocellular carcinoma
    Article Snippet: The PCR reaction was conducted at 95°C for 15 min, followed by 40 cycles at 95°C for 10 sec, 58°C for 20 sec and 72°C for 30 sec. RT-qPCR was performed on the ABI StepOnePlus™ thermocycler (Thermo Fisher Scientific, Inc.). .. U6 single nuclear (sn)RNA was employed as the loading control for micro (mi)RNA expression, and β-actin for the expression of genes; stem-loop RT primers were used for miRNAs, and oligo (dinucleotide) primers for genes.

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems). .. Relative expression was determined using the ΔΔCT method with samples normalized to the expression level of the cyclophilin A (CPHA) transcript.

    Cell Culture:

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems). .. Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems).

    Generated:

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: RNA was isolated from the 6-well plates using RNeasy Mini Kit (Qiagen, 74104) protocol with a final elution volume of 30μl RNAse free H2 0. cDNA was generated using QuantiTect Reverse Transcription (Qiagen, 205310), per manufacturer instructions. .. Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Differential protein‐coding gene and long noncoding RNA expression in smoking‐related lung squamous cell carcinoma
    Article Snippet: .. Total RNA was then reverse transcribed to cDNA using the Reverse Transcription Kit (Roche, Basel, Switzerland). qRT‐PCR reactions were performed using an ABI StepOnePlus Thermocycler using a SYBR Green PCR Kit (Thermo Fisher Scientific). ..

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: PCR was performed in triplicate in a 20-μl reaction volume containing 10 μl Go-Taq SYBR-Green PCR Master Mix (Applied Biosystems, Beijing, China), 10 µmol forward and reverse primers ( ; synthesized by Beijing Genomics Institute, Beijing, China), and 10 ng of gDNA. .. The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems).

    Article Title: Identification of differentially expressed protein-coding genes in lung adenocarcinomas
    Article Snippet: .. A FastQuant RT kit (with gDNase; Tiangen Biotech Co., Ltd.) was used to reverse transcribe total RNA to cDNA; the temperature of the RT process was 42°C. qPCR was performed in an ABI StepOnePlus Thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc.) using a SYBR Green PCR kit (Qiagen GmbH). ..

    Article Title: MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-κB
    Article Snippet: .. All quantitative RT-PCR reactions were performed in triplicates by an ABI StepOnePlus thermocycler (Applied Biosystems) with SYBR Green PCR Master Mix (Thermo Scientific). ..

    Article Title: Cabazitaxel-induced autophagy via the PI3K/Akt/mTOR pathway contributes to A549 cell death
    Article Snippet: .. Briefly, the total volume of 20 µ l reverse transcription mixer (500 ng RNA, 1 µ l oligo(dT)18, 1µ l RevertAid RT) was incubated for 60 min at 42°C, then the reaction was terminated by heating at 70°C for 5 min. qPCR was performed on an ABI StepOnePlus™ thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc., Foster City, CA, USA) using a SYBR® Green PCR kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. Each sample was run in triplicate in a final volume of 25 µ l containing 1 µ l first-strand cDNA template, 10 pmol of each primer ( ; obtained from Sangon Biotech Co., Ltd, Shanghai, China), 12.5 µ l of SYBR® Green PCR master mix and 11.5 µ l distilled water.

    Article Title: ACTG1 and TLR3 are biomarkers for alcohol-associated hepatocellular carcinoma
    Article Snippet: .. The PCR reaction was conducted at 95°C for 15 min, followed by 40 cycles at 95°C for 10 sec, 58°C for 20 sec and 72°C for 30 sec. RT-qPCR was performed on the ABI StepOnePlus™ thermocycler (Thermo Fisher Scientific, Inc.). .. U6 single nuclear (sn)RNA was employed as the loading control for micro (mi)RNA expression, and β-actin for the expression of genes; stem-loop RT primers were used for miRNAs, and oligo (dinucleotide) primers for genes.

    Article Title: Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro
    Article Snippet: The qRT-PCR was performed using an ABI StepOnePlus thermocycler (Applied Biosystems) using the Fast SYBR Green Master Mix. .. The reactions for serine/arginine-rich splicing factor 1 (SRSF1), TBP, and GAPDH were performed by two-step thermal cycling method: 95°C for 1 min, 40 PCR cycles at 95°C for 30 s, 59°C for 20 s, followed by generating melting curves to check the specificity of the reactions.

    Sequencing:

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems). .. After amplification, data were analyzed and exported by Sequence Detection Software (SDS) for the value of the threshold cycle (C t ), differences in C t values (ΔC t ) between the test locus and the control locus (GAPDH), and the comparative C t (ΔΔC t ) were used to calculate patient sample copy number value compared with the volunteer sample by relative quantitation (fold change).

    Article Title: PD-1 Dynamically Regulates Inflammation and Development of Brain-Resident Memory CD8 T Cells During Persistent Viral Encephalitis
    Article Snippet: Quantification of MuPyV Genomes and LT-Ag mRNA TaqMan real-time PCR was performed in an ABI StepOnePlus thermocycler (Applied Biosciences) with 10 μg of template DNA purified from tissues using the Maxwell 16 Research Instrument (Promega, Madison, WI) according to the manufacturer's instructions. .. Quantitative PCR (qPCR) was performed using FastStart Universal Probe Master (ROX) mix (Sigma-Aldrich) and an ABI PRISM 5700 sequence detection system (Applied Biosystems, Foster, CA), with protocol and primer set as previously described ( ).

    Binding Assay:

    Article Title: Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro
    Article Snippet: The qRT-PCR was performed using an ABI StepOnePlus thermocycler (Applied Biosystems) using the Fast SYBR Green Master Mix. .. Expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA box binding protein (TBP), and ribosomal protein, large, P0 (RPLP0) were used for normalization.

    DNA Extraction:

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: Fresh whole blood samples from patients 16 (analysis of TTYH3, AMZ1, and GNA12) and 17 (analysis of BC038729 and CARD11) as well as two age-matched healthy controls were taken and gDNA extracted using a blood genomic DNA extraction kit according to the manufacturer’s instructions (Generay Biotechnology, Shanghai, China). .. The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems).

    Isolation:

    Article Title: Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro
    Article Snippet: Paragraph title: RNA isolation and quantitative real-time polymerase chain reaction (qRT-PCR) ... The qRT-PCR was performed using an ABI StepOnePlus thermocycler (Applied Biosystems) using the Fast SYBR Green Master Mix.

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: RNA was isolated from the 6-well plates using RNeasy Mini Kit (Qiagen, 74104) protocol with a final elution volume of 30μl RNAse free H2 0. cDNA was generated using QuantiTect Reverse Transcription (Qiagen, 205310), per manufacturer instructions. .. Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems).

    Size-exclusion Chromatography:

    Article Title: Cabazitaxel-induced autophagy via the PI3K/Akt/mTOR pathway contributes to A549 cell death
    Article Snippet: Briefly, the total volume of 20 µ l reverse transcription mixer (500 ng RNA, 1 µ l oligo(dT)18, 1µ l RevertAid RT) was incubated for 60 min at 42°C, then the reaction was terminated by heating at 70°C for 5 min. qPCR was performed on an ABI StepOnePlus™ thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc., Foster City, CA, USA) using a SYBR® Green PCR kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. Cycling conditions of the qPCR were as follows: 1 cycle at 95°C for 30 sec, followed by 40 cycles at 95°C for 20 sec, 60°C for 30 sec and extension at 72°C for 15 sec.. Data analyses were performed according to the 2−ΔΔ Cq method ( ).

    Article Title: ACTG1 and TLR3 are biomarkers for alcohol-associated hepatocellular carcinoma
    Article Snippet: .. The PCR reaction was conducted at 95°C for 15 min, followed by 40 cycles at 95°C for 10 sec, 58°C for 20 sec and 72°C for 30 sec. RT-qPCR was performed on the ABI StepOnePlus™ thermocycler (Thermo Fisher Scientific, Inc.). .. U6 single nuclear (sn)RNA was employed as the loading control for micro (mi)RNA expression, and β-actin for the expression of genes; stem-loop RT primers were used for miRNAs, and oligo (dinucleotide) primers for genes.

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems). .. The amplification protocol was according to manufacturer's recommendation and included an initial denaturing step at 90°C for 10min, followed by 45 cycles with denaturation at 95°C for 15 sec and an annealing/elongation step of 60°C for 1min.

    Purification:

    Article Title: PD-1 Dynamically Regulates Inflammation and Development of Brain-Resident Memory CD8 T Cells During Persistent Viral Encephalitis
    Article Snippet: .. Quantification of MuPyV Genomes and LT-Ag mRNA TaqMan real-time PCR was performed in an ABI StepOnePlus thermocycler (Applied Biosciences) with 10 μg of template DNA purified from tissues using the Maxwell 16 Research Instrument (Promega, Madison, WI) according to the manufacturer's instructions. .. Primers and amplification parameters are previously described ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: Paragraph title: ELISA and RT-PCR Analysis ... Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems).

    Software:

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems). .. After amplification, data were analyzed and exported by Sequence Detection Software (SDS) for the value of the threshold cycle (C t ), differences in C t values (ΔC t ) between the test locus and the control locus (GAPDH), and the comparative C t (ΔΔC t ) were used to calculate patient sample copy number value compared with the volunteer sample by relative quantitation (fold change).

    Real-time Polymerase Chain Reaction:

    Article Title: Differential protein‐coding gene and long noncoding RNA expression in smoking‐related lung squamous cell carcinoma
    Article Snippet: Paragraph title: Quantitative real‐time PCR (qRT‐PCR) ... Total RNA was then reverse transcribed to cDNA using the Reverse Transcription Kit (Roche, Basel, Switzerland). qRT‐PCR reactions were performed using an ABI StepOnePlus Thermocycler using a SYBR Green PCR Kit (Thermo Fisher Scientific).

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: Paragraph title: Real-time quantitative PCR ... The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems).

    Article Title: Identification of differentially expressed protein-coding genes in lung adenocarcinomas
    Article Snippet: .. A FastQuant RT kit (with gDNase; Tiangen Biotech Co., Ltd.) was used to reverse transcribe total RNA to cDNA; the temperature of the RT process was 42°C. qPCR was performed in an ABI StepOnePlus Thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc.) using a SYBR Green PCR kit (Qiagen GmbH). ..

    Article Title: MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-κB
    Article Snippet: Paragraph title: Quantitative real-time-PCR (qRT-PCR) ... All quantitative RT-PCR reactions were performed in triplicates by an ABI StepOnePlus thermocycler (Applied Biosystems) with SYBR Green PCR Master Mix (Thermo Scientific).

    Article Title: Cabazitaxel-induced autophagy via the PI3K/Akt/mTOR pathway contributes to A549 cell death
    Article Snippet: .. Briefly, the total volume of 20 µ l reverse transcription mixer (500 ng RNA, 1 µ l oligo(dT)18, 1µ l RevertAid RT) was incubated for 60 min at 42°C, then the reaction was terminated by heating at 70°C for 5 min. qPCR was performed on an ABI StepOnePlus™ thermocycler (Applied Biosystems; Thermo Fisher Scientific, Inc., Foster City, CA, USA) using a SYBR® Green PCR kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. Each sample was run in triplicate in a final volume of 25 µ l containing 1 µ l first-strand cDNA template, 10 pmol of each primer ( ; obtained from Sangon Biotech Co., Ltd, Shanghai, China), 12.5 µ l of SYBR® Green PCR master mix and 11.5 µ l distilled water.

    Article Title: PD-1 Dynamically Regulates Inflammation and Development of Brain-Resident Memory CD8 T Cells During Persistent Viral Encephalitis
    Article Snippet: .. Quantification of MuPyV Genomes and LT-Ag mRNA TaqMan real-time PCR was performed in an ABI StepOnePlus thermocycler (Applied Biosciences) with 10 μg of template DNA purified from tissues using the Maxwell 16 Research Instrument (Promega, Madison, WI) according to the manufacturer's instructions. .. Primers and amplification parameters are previously described ( ).

    Article Title: Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro
    Article Snippet: Paragraph title: RNA isolation and quantitative real-time polymerase chain reaction (qRT-PCR) ... The qRT-PCR was performed using an ABI StepOnePlus thermocycler (Applied Biosystems) using the Fast SYBR Green Master Mix.

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: .. Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems). .. The amplification protocol was according to manufacturer's recommendation and included an initial denaturing step at 90°C for 10min, followed by 45 cycles with denaturation at 95°C for 15 sec and an annealing/elongation step of 60°C for 1min.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles
    Article Snippet: Paragraph title: ELISA and RT-PCR Analysis ... Real time PCR amplification was performed using a final volume of 20μl containing 3μl CDNA, 2μl Primer Mix (5μM each forward and reverse, Eurofins MWG Operon), 5μl RNAse free H2 O and 10μl POWER SYBR Green (Applied Biosystems) on a ABI StepOnePlus thermocycler (Applied Biosystems).

    Quantitation Assay:

    Article Title: Rare copy number variants in the genome of Chinese female children and adolescents with Turner syndrome
    Article Snippet: The reaction cycling conditions were 95°C for 2 min, followed by 40 cycles at 95°C for 15 s, and at 60°C for 30 s in an ABI StepOnePlus thermocycler (Applied Biosystems). .. After amplification, data were analyzed and exported by Sequence Detection Software (SDS) for the value of the threshold cycle (C t ), differences in C t values (ΔC t ) between the test locus and the control locus (GAPDH), and the comparative C t (ΔΔC t ) were used to calculate patient sample copy number value compared with the volunteer sample by relative quantitation (fold change).

    FACS:

    Article Title: PD-1 Dynamically Regulates Inflammation and Development of Brain-Resident Memory CD8 T Cells During Persistent Viral Encephalitis
    Article Snippet: Quantification of MuPyV Genomes and LT-Ag mRNA TaqMan real-time PCR was performed in an ABI StepOnePlus thermocycler (Applied Biosciences) with 10 μg of template DNA purified from tissues using the Maxwell 16 Research Instrument (Promega, Madison, WI) according to the manufacturer's instructions. .. To determine MuPyV LT-Ag mRNA copy numbers, total RNA from FACS-purified cells was insolated using TRIzol (Ambion, USA) and cDNA was prepared using RevertAid H minus reverse transcriptase (Thermo Scientific, Waltham, MA), as per manufacturer's instructions.

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    Thermo Fisher abi steponeplus thermocycler
    Abi Steponeplus Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abi Steponeplus Real Time Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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