abi steponeplus real time thermalcycler  (Thermo Fisher)


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    Structured Review

    Thermo Fisher abi steponeplus real time thermalcycler
    Abi Steponeplus Real Time Thermalcycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus real time thermalcycler/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus real time thermalcycler - by Bioz Stars, 2020-03
    82/100 stars

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    real-time pcr assays

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    Related Articles

    Isolation:

    Article Title: MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells
    Article Snippet: Reverse transcriptase PCR (RT-PCR) and real-time PCR Total RNA was isolated from fine dissected OC isolated from P10 inner ear tissue of wild-type, heterozygous and Map3k1tm1Yxia homozygous mice (five mice each) using the RiboPure RNA isolation kit (Life Technologies, Grand Island, NY) and cDNA was prepared using an oligo-dT primer and SMARTScribe Reverse Transcriptase enzymes (Clontech, Mountain View, CA). .. The real-time PCR assays were performed in triplicate using an ABI StepOnePlus Real-Time thermalcycler (Life Technologies, Grand Island, NY).

    Mouse Assay:

    Article Title: MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells
    Article Snippet: Reverse transcriptase PCR (RT-PCR) and real-time PCR Total RNA was isolated from fine dissected OC isolated from P10 inner ear tissue of wild-type, heterozygous and Map3k1tm1Yxia homozygous mice (five mice each) using the RiboPure RNA isolation kit (Life Technologies, Grand Island, NY) and cDNA was prepared using an oligo-dT primer and SMARTScribe Reverse Transcriptase enzymes (Clontech, Mountain View, CA). .. The real-time PCR assays were performed in triplicate using an ABI StepOnePlus Real-Time thermalcycler (Life Technologies, Grand Island, NY).

    Real-time Polymerase Chain Reaction:

    Article Title: MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells
    Article Snippet: .. The real-time PCR assays were performed in triplicate using an ABI StepOnePlus Real-Time thermalcycler (Life Technologies, Grand Island, NY). .. CT values were normalized using Gapdh and actin as an endogenous control, and fold changes of different genes were calculated using SABiosciences online software ( http://pcrdataanalysis.sabiosciences.com/pcr/arrayanalysis.php ).

    Polymerase Chain Reaction:

    Article Title: MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells
    Article Snippet: Paragraph title: Reverse transcriptase PCR (RT-PCR) and real-time PCR ... The real-time PCR assays were performed in triplicate using an ABI StepOnePlus Real-Time thermalcycler (Life Technologies, Grand Island, NY).

    Expressing:

    Article Title: MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells
    Article Snippet: To determine the differential expression of various genes, SYBR-Green-based real-time primers (available upon request) were designed using Integrated DNA Technologies online PrimeTime qPCR assay design tool ( http://www.idtdna.com/Scitools/Applications/RealTimePCR/ ). .. The real-time PCR assays were performed in triplicate using an ABI StepOnePlus Real-Time thermalcycler (Life Technologies, Grand Island, NY).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells
    Article Snippet: Paragraph title: Reverse transcriptase PCR (RT-PCR) and real-time PCR ... The real-time PCR assays were performed in triplicate using an ABI StepOnePlus Real-Time thermalcycler (Life Technologies, Grand Island, NY).

    Software:

    Article Title: MAP3K1 function is essential for cytoarchitecture of the mouse organ of Corti and survival of auditory hair cells
    Article Snippet: The real-time PCR assays were performed in triplicate using an ABI StepOnePlus Real-Time thermalcycler (Life Technologies, Grand Island, NY). .. CT values were normalized using Gapdh and actin as an endogenous control, and fold changes of different genes were calculated using SABiosciences online software ( http://pcrdataanalysis.sabiosciences.com/pcr/arrayanalysis.php ).

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  • 88
    Thermo Fisher abi steponeplus real time thermocycler
    Abi Steponeplus Real Time Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus real time thermocycler/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus real time thermocycler - by Bioz Stars, 2020-03
    88/100 stars
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