abi steponeplus pcr machine  (Thermo Fisher)


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    Structured Review

    Thermo Fisher abi steponeplus pcr machine
    Abi Steponeplus Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi steponeplus pcr machine/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus pcr machine - by Bioz Stars, 2020-04
    89/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: Paragraph title: Analysis of P2X7 R splice isoform expression by quantitative-polymerase chain reaction (q-PCR) ... All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation).

    Amplification:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: A melt curve was also performed for all primers to ensure that they recognized and amplified a single product. qPCR reactions were performed using a SensiFAST no-ROX Kit (Life Technologies) according to the manufacturer’s instructions. .. All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation).

    Agarose Gel Electrophoresis:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation). .. PCR samples along with a 100 bp interval ladder (BIOLINE, London, UK) were separated on a 1% agarose gel with ethidium bromide dissolved in TBE buffer (Promega).

    Isolation:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: 2.9 Analysis of P2X7 R splice isoform expression by quantitative-polymerase chain reaction (q-PCR) Total RNA was extracted from the cell populations of interest using an RNA isolation system (TRIzol RNA minikit, Invitrogen) according to the manufacturer’s instructions and subsequently treated with DNase (Ambion, Life Technologies, Carlsbad, California, USA). .. All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation).

    SYBR Green Assay:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: A dye based (SYBR Green) qPCR was used to obtain relative quantification of mRNA levels of the various P2X7 R isoforms of interest relative to the housekeeping gene hypoxanthine–guanine phosphoribosyltransferase (HRPT). (HPRT: forward: GGG CTT ACC TCA CTG CTT TC, reverse: TCT CCA CCA ATA ACT TTT ATG TCC; P2X7 A: forward: CAC ATG ATC GTC TTT TCC TAC, reverse: GGT CAG AAG AGC ACT GTG C; P2X7 K: forward: GCC CGT GAG CCA CTT ATG C reverse: GGT CAG AAG AGC ACT GTG C. All primers were obtained from Sigma–Aldrich and were checked against GenBank for selectivity. .. All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation).

    Polymerase Chain Reaction:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: .. All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation). .. Samples were considered positive if the amplification curve crossed the set threshold (set automatically).

    Expressing:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: Paragraph title: Analysis of P2X7 R splice isoform expression by quantitative-polymerase chain reaction (q-PCR) ... All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation).

    CTG Assay:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: A dye based (SYBR Green) qPCR was used to obtain relative quantification of mRNA levels of the various P2X7 R isoforms of interest relative to the housekeeping gene hypoxanthine–guanine phosphoribosyltransferase (HRPT). (HPRT: forward: GGG CTT ACC TCA CTG CTT TC, reverse: TCT CCA CCA ATA ACT TTT ATG TCC; P2X7 A: forward: CAC ATG ATC GTC TTT TCC TAC, reverse: GGT CAG AAG AGC ACT GTG C; P2X7 K: forward: GCC CGT GAG CCA CTT ATG C reverse: GGT CAG AAG AGC ACT GTG C. All primers were obtained from Sigma–Aldrich and were checked against GenBank for selectivity. .. All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation).

    Activated Clotting Time Assay:

    Article Title: P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages
    Article Snippet: A dye based (SYBR Green) qPCR was used to obtain relative quantification of mRNA levels of the various P2X7 R isoforms of interest relative to the housekeeping gene hypoxanthine–guanine phosphoribosyltransferase (HRPT). (HPRT: forward: GGG CTT ACC TCA CTG CTT TC, reverse: TCT CCA CCA ATA ACT TTT ATG TCC; P2X7 A: forward: CAC ATG ATC GTC TTT TCC TAC, reverse: GGT CAG AAG AGC ACT GTG C; P2X7 K: forward: GCC CGT GAG CCA CTT ATG C reverse: GGT CAG AAG AGC ACT GTG C. All primers were obtained from Sigma–Aldrich and were checked against GenBank for selectivity. .. All samples were run in triplicate in an ABI StepOnePlus PCR machine (Applied Biosystems, Life Technologies) using the following conditions: 95 °C for 2 min and then 40 cycles at: 95 °C for 5 s (denaturation), 60–65 °C for 10 s (annealing) and 72 °C for 5–20 s (elongation).

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    Thermo Fisher abi steponeplus real time pcr machine
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