abi prism bigdye terminator v3 1 cycle sequencing kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher abi prism bigdye terminator v3 1 cycle sequencing kit
    Abi Prism Bigdye Terminator V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 152 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator v3 1 cycle sequencing kit - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish Samples
    Article Snippet: Plasmid DNA from each of 100 clones was purified by using the Wizard Plus SV Minipreps kit (Promega). .. Nucleotide sequencing was carried out in an ABI PRISM 377 automated DNA sequencer, the ABI PRISM BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems), and vector-derived primers.

    Polymerase Chain Reaction:

    Article Title: In Vitro Human Immunodeficiency Virus Type 1 Resistance Selections with Combinations of Tenofovir and Emtricitabine or Abacavir and Lamivudine ▿
    Article Snippet: .. HIV-1 RNA was extracted from virus supernatants (viral RNA preparation kit; QIAGEN, Valencia, CA), and the PCR products obtained after reverse transcription (Titan RT-PCR; Roche Diagnostics, Indianapolis, IN) were analyzed by population-based sequencing with an ABI Prism BigDye Terminator v3.1 cycle sequencing kit and a 3100 ABI Prism genetic analyzer (Applied Biosystems, Foster City, CA). .. The RT sequence corresponding to amino acids 1 to 300 was assessed.

    Article Title: Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish Samples
    Article Snippet: Digested DNAs were purified with the High Pure PCR product purification kit (Roche) according to the directions of the manufacturer. .. Nucleotide sequencing was carried out in an ABI PRISM 377 automated DNA sequencer, the ABI PRISM BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems), and vector-derived primers.

    Article Title: The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket
    Article Snippet: Constructs encoding epitope-tagged C-terminal ORDs of OSBP and ORPs 1–11 were created by PCR and encompassed the following amino acid residues of the respective proteins: OSBP, 304–809; ORP1, 514–950; ORP2, 1–480; ORP3, 399–886; ORP4 , 413–916; ORP5 , 266–826; ORP6, 426–934; ORP7, 364–842; ORP8, 242–793; ORP9, 268–736; ORP10, 306–764; ORP11, 274–747. .. All constructs were verified by sequencing using ABI PRISM BigDye™ Terminator v3.1 Cycle Sequencing kit and ABI3730 DNA analyser (Applied Biosystems, Foster City, CA, U.S.A.).

    Mutagenesis:

    Article Title: The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket
    Article Snippet: Paragraph title: cDNA constructs and site-directed mutagenesis ... All constructs were verified by sequencing using ABI PRISM BigDye™ Terminator v3.1 Cycle Sequencing kit and ABI3730 DNA analyser (Applied Biosystems, Foster City, CA, U.S.A.).

    Construct:

    Article Title: The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket
    Article Snippet: .. All constructs were verified by sequencing using ABI PRISM BigDye™ Terminator v3.1 Cycle Sequencing kit and ABI3730 DNA analyser (Applied Biosystems, Foster City, CA, U.S.A.). .. The amino acid sequences of all human and mouse ORP proteins as well as those from the yeast S. cerevisiae were employed to construct a multiple sequence alignment by using ClustalW (version 1.82) [ ].

    Purification:

    Article Title: Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish Samples
    Article Snippet: Plasmid DNA from each of 100 clones was purified by using the Wizard Plus SV Minipreps kit (Promega). .. Nucleotide sequencing was carried out in an ABI PRISM 377 automated DNA sequencer, the ABI PRISM BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems), and vector-derived primers.

    Produced:

    Article Title: Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish Samples
    Article Snippet: Since the DNA fragments produced by Pwo polymerase are blunt ended, a single digestion with the BamHI enzyme was necessary for the amplimer, while the plasmid vector was digested with both BamHI and HincII restriction enzymes. .. Nucleotide sequencing was carried out in an ABI PRISM 377 automated DNA sequencer, the ABI PRISM BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems), and vector-derived primers.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: In Vitro Human Immunodeficiency Virus Type 1 Resistance Selections with Combinations of Tenofovir and Emtricitabine or Abacavir and Lamivudine ▿
    Article Snippet: .. HIV-1 RNA was extracted from virus supernatants (viral RNA preparation kit; QIAGEN, Valencia, CA), and the PCR products obtained after reverse transcription (Titan RT-PCR; Roche Diagnostics, Indianapolis, IN) were analyzed by population-based sequencing with an ABI Prism BigDye Terminator v3.1 cycle sequencing kit and a 3100 ABI Prism genetic analyzer (Applied Biosystems, Foster City, CA). .. The RT sequence corresponding to amino acids 1 to 300 was assessed.

    Expressing:

    Article Title: The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket
    Article Snippet: For live cell photo-cross-linking experiments, all of the cDNAs were subcloned into the pcDNA4HisMaxC expression vector (Invitrogen), which encodes hexahistidine and Xpress™ epitope tags fused at the N-terminus of the insert-encoded proteins. .. All constructs were verified by sequencing using ABI PRISM BigDye™ Terminator v3.1 Cycle Sequencing kit and ABI3730 DNA analyser (Applied Biosystems, Foster City, CA, U.S.A.).

    Sequencing:

    Article Title: In Vitro Human Immunodeficiency Virus Type 1 Resistance Selections with Combinations of Tenofovir and Emtricitabine or Abacavir and Lamivudine ▿
    Article Snippet: .. HIV-1 RNA was extracted from virus supernatants (viral RNA preparation kit; QIAGEN, Valencia, CA), and the PCR products obtained after reverse transcription (Titan RT-PCR; Roche Diagnostics, Indianapolis, IN) were analyzed by population-based sequencing with an ABI Prism BigDye Terminator v3.1 cycle sequencing kit and a 3100 ABI Prism genetic analyzer (Applied Biosystems, Foster City, CA). .. The RT sequence corresponding to amino acids 1 to 300 was assessed.

    Article Title: Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish Samples
    Article Snippet: .. Nucleotide sequencing was carried out in an ABI PRISM 377 automated DNA sequencer, the ABI PRISM BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems), and vector-derived primers. ..

    Article Title: The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket
    Article Snippet: .. All constructs were verified by sequencing using ABI PRISM BigDye™ Terminator v3.1 Cycle Sequencing kit and ABI3730 DNA analyser (Applied Biosystems, Foster City, CA, U.S.A.). .. The amino acid sequences of all human and mouse ORP proteins as well as those from the yeast S. cerevisiae were employed to construct a multiple sequence alignment by using ClustalW (version 1.82) [ ].

    Variant Assay:

    Article Title: The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket
    Article Snippet: Human ORP1L, ORP1S (short variant consisting of an ORD only) (=ORP1 ORD) and ORP2 cDNAs were subcloned into the BamHI site of pGex-1λT (GE Healthcare, Little Chalfont, Bucks., U.K.). .. All constructs were verified by sequencing using ABI PRISM BigDye™ Terminator v3.1 Cycle Sequencing kit and ABI3730 DNA analyser (Applied Biosystems, Foster City, CA, U.S.A.).

    Plasmid Preparation:

    Article Title: Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish Samples
    Article Snippet: .. Nucleotide sequencing was carried out in an ABI PRISM 377 automated DNA sequencer, the ABI PRISM BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems), and vector-derived primers. ..

    Article Title: The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket
    Article Snippet: For live cell photo-cross-linking experiments, all of the cDNAs were subcloned into the pcDNA4HisMaxC expression vector (Invitrogen), which encodes hexahistidine and Xpress™ epitope tags fused at the N-terminus of the insert-encoded proteins. .. All constructs were verified by sequencing using ABI PRISM BigDye™ Terminator v3.1 Cycle Sequencing kit and ABI3730 DNA analyser (Applied Biosystems, Foster City, CA, U.S.A.).

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    Thermo Fisher abi prism bigdye terminator v3 1 cycle sequencing kit
    Abi Prism Bigdye Terminator V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 90 stars, based on 152 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator v3 1 cycle sequencing kit - by Bioz Stars, 2020-02
    90/100 stars
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