abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit  (Thermo Fisher)


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    Thermo Fisher abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit
    Abi Prism Bigdye Terminator V3 0 Ready Reaction Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Methylation Sequencing:

    Article Title: Epigenetic regulation of MDR1 gene through post-translational histone modifications in prostate cancer
    Article Snippet: Paragraph title: Bisulfite sequencing ... The purified products were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) in an ABI PRISMTM 310 Genetic Analyzer (Applied Biosystems).

    Article Title: The novel colorectal cancer biomarkers CDO1, ZSCAN18 and ZNF331 are frequently methylated across gastrointestinal cancers
    Article Snippet: Paragraph title: Direct bisulfite sequencing ... Briefly, fragments were amplified for 35 cycles using HotStarTaq (Qiagen) and the purified samples were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit in an AB Prism 3730 sequencer (Life Technologies).

    Clone Assay:

    Article Title: Cloning and expression of a novel catechol-O-methyltransferase in common marmosets
    Article Snippet: Paragraph title: COMT cDNA cloning ... The sequence verification of insert was carried out using an ABI PRISM BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) with an ABI PRISM 3730 DNA Analyzer (Applied Biosystems).

    Amplification:

    Article Title: Association of rheumatic fever rheumatic heart disease with plausible early late-stage disease markers
    Article Snippet: The amplified products were purified by HiYield™ polymerase chain reaction DNA purification kit (Real Biotech Corporation). .. The purified products were processed for sequencing reactions in ABI PRISM® dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing kit (Applied Biosystems, USA) following manufacturer's instructions.

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: .. The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310. .. YWHAE–FAM22A –FLAG fusion cDNA containing BamHI ( YWHAE end) and EcoRI (FLAG end) restriction sites was synthesized (GenScript) based on the sequences of the fusion transcript present in ESS1 and cloned in pUC57 vector.

    Article Title: Genetic Characterization of Legionella pneumophila Isolated from a Common Watershed in Comunidad Valenciana, Spain
    Article Snippet: DNA Sequencing Purified PCR products were subjected to Sanger sequencing using BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) and analyzed in an ABI PRISM 3700 sequencer (Applied Biosystems, Foster City, CA). .. The oligonucleotides used for sequencing were the same used in the amplification reaction except for mompS .

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: Amplified products were purified with the Promega Wizard SV Gel and PCR Clean up System following the manufacturer’s protocol. .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation.

    Article Title: The novel colorectal cancer biomarkers CDO1, ZSCAN18 and ZNF331 are frequently methylated across gastrointestinal cancers
    Article Snippet: .. Briefly, fragments were amplified for 35 cycles using HotStarTaq (Qiagen) and the purified samples were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit in an AB Prism 3730 sequencer (Life Technologies). .. The electroferograms were analyzed manually using Sequencing Analysis 5.2 (Life Technologies).

    Article Title: Species Delimitation in the Genus Moschus (Ruminantia: Moschidae) and Its High-Plateau Origin
    Article Snippet: .. The amplification protocol included an initial denaturation step of 95°C for 5 min; this was followed by 32 cycles of denaturation at 95°C for 30 s, primer annealing at 55°C for 30 s, and an extension at 72°C for 80 s, with a final extension at 72°C for 10 min. PCR products were purified using an EasyPure PCR Purification Kit (TransGene), and sequenced using previous primers and the BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) following the manufacturer’s instructions on an ABI Prism 3730 automated sequencer. ..

    Article Title: Population Genetic Structure of the Staphylococcus intermedius Group: Insights into agr Diversification and the Emergence of Methicillin-Resistant Strains ▿ Diversification and the Emergence of Methicillin-Resistant Strains ▿ †
    Article Snippet: Sequencing reactions were carried out using 5 μl purified PCR-amplified DNA (approximately 50 to 90 ng) plus 1 μl sequencing primer (3.2 pmol/μl) with the BigDye Terminator v3.0 Ready Reaction cycle sequencing kit (Applied Biosystems) according to the manufacturer's instructions. .. For the 16S rRNA, cpn60 , tuf , and pta genes, forward and reverse sequencing reactions were carried out with independently amplified PCR products to rule out the possibility of Taq polymerase-generated errors.

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: Isolation and sequencing of M. domestica IGF2 A Monodelphis domestica genomic library constructed in Lamda FIX® II/Xho I (Stratagene, according to the manufacturer's protocol) was screened using a probe generated from amplification of genomic DNA from a male M. domestica liver with primers designed from an alignment of the 3' untranslated region (UTR) of several species [ ]. .. Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    Filtration:

    Article Title: A novel mutation in the WFS1 gene identified in a Taiwanese family with low-frequency hearing impairment
    Article Snippet: Direct sequencing A 10-μL sequencing reaction was performed with 100 ng of the purified PCR product as a template, 30 pmol of each of the sequencing primers, and 1 μL of the BigDye Terminator v3.0 Sequencing Ready Reaction Cycle sequencing kit (Applied Biosystems). .. After completion of the reaction, the mixture was purified by gel filtration on a Sephadex G-50 column (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

    Polymerase Chain Reaction:

    Article Title: Association of rheumatic fever rheumatic heart disease with plausible early late-stage disease markers
    Article Snippet: The amplified products were purified by HiYield™ polymerase chain reaction DNA purification kit (Real Biotech Corporation). .. The purified products were processed for sequencing reactions in ABI PRISM® dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing kit (Applied Biosystems, USA) following manufacturer's instructions.

    Article Title: Epigenetic regulation of MDR1 gene through post-translational histone modifications in prostate cancer
    Article Snippet: Excess primer and nucleotides were removed by Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare, USB Corporation, Cleveland, OH) following the protocol of the manufacturer. .. The purified products were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) in an ABI PRISMTM 310 Genetic Analyzer (Applied Biosystems).

    Article Title: Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W]Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W] [OA]
    Article Snippet: For PCR from genomic DNA, primers were designed using the web-based Primer3 program , and PCR products were separated on agarose gels and then purified using a gel extraction kit (Qiagen). .. Sequencing of purified DNA was performed using the Big-Dye Terminator v3.0 ready reaction cycle sequencing kit (Applied Biosystems) and an ABI 3100 automated DNA sequencer (Applied Biosystems).

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: .. The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310. .. YWHAE–FAM22A –FLAG fusion cDNA containing BamHI ( YWHAE end) and EcoRI (FLAG end) restriction sites was synthesized (GenScript) based on the sequences of the fusion transcript present in ESS1 and cloned in pUC57 vector.

    Article Title: Genetic Characterization of Legionella pneumophila Isolated from a Common Watershed in Comunidad Valenciana, Spain
    Article Snippet: .. DNA Sequencing Purified PCR products were subjected to Sanger sequencing using BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) and analyzed in an ABI PRISM 3700 sequencer (Applied Biosystems, Foster City, CA). .. The oligonucleotides used for sequencing were the same used in the amplification reaction except for mompS .

    Article Title: Cloning and expression of a novel catechol-O-methyltransferase in common marmosets
    Article Snippet: Purified PCR products were cloned into pGEM-T easy vectors using a pGEM-T Easy Vector System (Promega, Madison, WI, U.S.A.). .. The sequence verification of insert was carried out using an ABI PRISM BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) with an ABI PRISM 3730 DNA Analyzer (Applied Biosystems).

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: Amplified products were purified with the Promega Wizard SV Gel and PCR Clean up System following the manufacturer’s protocol. .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation.

    Article Title: A novel mutation in the WFS1 gene identified in a Taiwanese family with low-frequency hearing impairment
    Article Snippet: .. Direct sequencing A 10-μL sequencing reaction was performed with 100 ng of the purified PCR product as a template, 30 pmol of each of the sequencing primers, and 1 μL of the BigDye Terminator v3.0 Sequencing Ready Reaction Cycle sequencing kit (Applied Biosystems). .. After completion of the reaction, the mixture was purified by gel filtration on a Sephadex G-50 column (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

    Article Title: Species Delimitation in the Genus Moschus (Ruminantia: Moschidae) and Its High-Plateau Origin
    Article Snippet: .. The amplification protocol included an initial denaturation step of 95°C for 5 min; this was followed by 32 cycles of denaturation at 95°C for 30 s, primer annealing at 55°C for 30 s, and an extension at 72°C for 80 s, with a final extension at 72°C for 10 min. PCR products were purified using an EasyPure PCR Purification Kit (TransGene), and sequenced using previous primers and the BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) following the manufacturer’s instructions on an ABI Prism 3730 automated sequencer. ..

    Article Title: Population Genetic Structure of the Staphylococcus intermedius Group: Insights into agr Diversification and the Emergence of Methicillin-Resistant Strains ▿ Diversification and the Emergence of Methicillin-Resistant Strains ▿ †
    Article Snippet: .. Sequencing reactions were carried out using 5 μl purified PCR-amplified DNA (approximately 50 to 90 ng) plus 1 μl sequencing primer (3.2 pmol/μl) with the BigDye Terminator v3.0 Ready Reaction cycle sequencing kit (Applied Biosystems) according to the manufacturer's instructions. ..

    Construct:

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: Isolation and sequencing of M. domestica IGF2 A Monodelphis domestica genomic library constructed in Lamda FIX® II/Xho I (Stratagene, according to the manufacturer's protocol) was screened using a probe generated from amplification of genomic DNA from a male M. domestica liver with primers designed from an alignment of the 3' untranslated region (UTR) of several species [ ]. .. Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    Electrophoresis:

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: The PCR products were visualised by means of electrophoresis in 1% agarose gel previously stained with ethidium bromide before UV exposure to verify amplification. .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation.

    Modification:

    Article Title: Epigenetic regulation of MDR1 gene through post-translational histone modifications in prostate cancer
    Article Snippet: Bisulfite sequencing Bisulfite modified DNA from three different PCa cell lines (LNCaP, DU145 and PC3), exposed to DAC and/or TSA as abovementioned, was used to evaluate the methylation status of CG dinucleotides, by bisulfite sequencing using primers for a specific sequence of MDR1 promoter, addressing the same region that was further analyzed by qMSP [ ]. .. The purified products were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) in an ABI PRISMTM 310 Genetic Analyzer (Applied Biosystems).

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: Polymerase chain reaction (PCR) was used to amplify a 709 bp Cyt. b fragment using the primers CP1 (5'-GATGATGAAATTTTGGATC-3') (modified from Harry et al [ ]) and Tser (5'-TATTTCTTTATTATGTTTTCAAAAC-3') [ ]. .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation.

    Western Blot:

    Article Title: Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W]Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W] [OA]
    Article Snippet: As the secondary antibody, horseradish peroxidase-labeled antibody was used, and the chemiluminescence signal was detected using ECL western blotting detection reagents (Amersham Biosciences). .. Sequencing of purified DNA was performed using the Big-Dye Terminator v3.0 ready reaction cycle sequencing kit (Applied Biosystems) and an ABI 3100 automated DNA sequencer (Applied Biosystems).

    Gel Purification:

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: .. The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310. .. YWHAE–FAM22A –FLAG fusion cDNA containing BamHI ( YWHAE end) and EcoRI (FLAG end) restriction sites was synthesized (GenScript) based on the sequences of the fusion transcript present in ESS1 and cloned in pUC57 vector.

    Ligation:

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: Subclones were generated by restriction digests with BamH I , EcoR I , Not I , and Sac II and ligation into pBluescript® II KS+ vector (Stratagene). .. Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    Generated:

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: Subclones were generated by restriction digests with BamH I , EcoR I , Not I , and Sac II and ligation into pBluescript® II KS+ vector (Stratagene). .. Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    DNA Sequencing:

    Article Title: Genetic Characterization of Legionella pneumophila Isolated from a Common Watershed in Comunidad Valenciana, Spain
    Article Snippet: .. DNA Sequencing Purified PCR products were subjected to Sanger sequencing using BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) and analyzed in an ABI PRISM 3700 sequencer (Applied Biosystems, Foster City, CA). .. The oligonucleotides used for sequencing were the same used in the amplification reaction except for mompS .

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation. ..

    Article Title: Population Genetic Structure of the Staphylococcus intermedius Group: Insights into agr Diversification and the Emergence of Methicillin-Resistant Strains ▿ Diversification and the Emergence of Methicillin-Resistant Strains ▿ †
    Article Snippet: Paragraph title: DNA sequencing. ... Sequencing reactions were carried out using 5 μl purified PCR-amplified DNA (approximately 50 to 90 ng) plus 1 μl sequencing primer (3.2 pmol/μl) with the BigDye Terminator v3.0 Ready Reaction cycle sequencing kit (Applied Biosystems) according to the manufacturer's instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: Paragraph title: RT-PCR and Sequencing. ... The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310.

    DNA Extraction:

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: Paragraph title: 2.3. DNA Extraction and Sequence Analysis ... DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation.

    Article Title: Species Delimitation in the Genus Moschus (Ruminantia: Moschidae) and Its High-Plateau Origin
    Article Snippet: Paragraph title: Specimen Collection, DNA Extraction, PCR Amplification, and Sequencing ... The amplification protocol included an initial denaturation step of 95°C for 5 min; this was followed by 32 cycles of denaturation at 95°C for 30 s, primer annealing at 55°C for 30 s, and an extension at 72°C for 80 s, with a final extension at 72°C for 10 min. PCR products were purified using an EasyPure PCR Purification Kit (TransGene), and sequenced using previous primers and the BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) following the manufacturer’s instructions on an ABI Prism 3730 automated sequencer.

    DNA Purification:

    Article Title: Association of rheumatic fever rheumatic heart disease with plausible early late-stage disease markers
    Article Snippet: The amplified products were purified by HiYield™ polymerase chain reaction DNA purification kit (Real Biotech Corporation). .. The purified products were processed for sequencing reactions in ABI PRISM® dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing kit (Applied Biosystems, USA) following manufacturer's instructions.

    Methylation:

    Article Title: Epigenetic regulation of MDR1 gene through post-translational histone modifications in prostate cancer
    Article Snippet: Bisulfite sequencing Bisulfite modified DNA from three different PCa cell lines (LNCaP, DU145 and PC3), exposed to DAC and/or TSA as abovementioned, was used to evaluate the methylation status of CG dinucleotides, by bisulfite sequencing using primers for a specific sequence of MDR1 promoter, addressing the same region that was further analyzed by qMSP [ ]. .. The purified products were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) in an ABI PRISMTM 310 Genetic Analyzer (Applied Biosystems).

    Article Title: The novel colorectal cancer biomarkers CDO1, ZSCAN18 and ZNF331 are frequently methylated across gastrointestinal cancers
    Article Snippet: Briefly, fragments were amplified for 35 cycles using HotStarTaq (Qiagen) and the purified samples were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit in an AB Prism 3730 sequencer (Life Technologies). .. The approximate amount of methylation at each CpG site was calculated by dividing the peak height of the cytosine signal with the peak height of the cytosine plus the thymine signal, giving values ranging from 0 to 1.

    Isolation:

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: RNAs from frozen tumor and cell line samples were extracted with a mirVana miRNA Isolation Kit (Ambion) according to the manufacturer's protocol. .. The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310.

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: Paragraph title: Isolation and sequencing of M. domestica IGF2 ... Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    Purification:

    Article Title: Association of rheumatic fever rheumatic heart disease with plausible early late-stage disease markers
    Article Snippet: .. The purified products were processed for sequencing reactions in ABI PRISM® dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing kit (Applied Biosystems, USA) following manufacturer's instructions. .. Finally, the sequencing was carried out in 3130×l genetic analyzer of Applied Biosystems.

    Article Title: Epigenetic regulation of MDR1 gene through post-translational histone modifications in prostate cancer
    Article Snippet: .. The purified products were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) in an ABI PRISMTM 310 Genetic Analyzer (Applied Biosystems). ..

    Article Title: Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W]Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W] [OA]
    Article Snippet: .. Sequencing of purified DNA was performed using the Big-Dye Terminator v3.0 ready reaction cycle sequencing kit (Applied Biosystems) and an ABI 3100 automated DNA sequencer (Applied Biosystems). .. PCR conditions for previously available markers were obtained from The Arabidopsis Information Resource, and restriction enzymes used for each marker are listed in Supplemental Table S2.

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: .. The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310. .. YWHAE–FAM22A –FLAG fusion cDNA containing BamHI ( YWHAE end) and EcoRI (FLAG end) restriction sites was synthesized (GenScript) based on the sequences of the fusion transcript present in ESS1 and cloned in pUC57 vector.

    Article Title: Genetic Characterization of Legionella pneumophila Isolated from a Common Watershed in Comunidad Valenciana, Spain
    Article Snippet: .. DNA Sequencing Purified PCR products were subjected to Sanger sequencing using BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) and analyzed in an ABI PRISM 3700 sequencer (Applied Biosystems, Foster City, CA). .. The oligonucleotides used for sequencing were the same used in the amplification reaction except for mompS .

    Article Title: Cloning and expression of a novel catechol-O-methyltransferase in common marmosets
    Article Snippet: Purified PCR products were cloned into pGEM-T easy vectors using a pGEM-T Easy Vector System (Promega, Madison, WI, U.S.A.). .. The sequence verification of insert was carried out using an ABI PRISM BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) with an ABI PRISM 3730 DNA Analyzer (Applied Biosystems).

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: Amplified products were purified with the Promega Wizard SV Gel and PCR Clean up System following the manufacturer’s protocol. .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation.

    Article Title: A novel mutation in the WFS1 gene identified in a Taiwanese family with low-frequency hearing impairment
    Article Snippet: .. Direct sequencing A 10-μL sequencing reaction was performed with 100 ng of the purified PCR product as a template, 30 pmol of each of the sequencing primers, and 1 μL of the BigDye Terminator v3.0 Sequencing Ready Reaction Cycle sequencing kit (Applied Biosystems). .. After completion of the reaction, the mixture was purified by gel filtration on a Sephadex G-50 column (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

    Article Title: The novel colorectal cancer biomarkers CDO1, ZSCAN18 and ZNF331 are frequently methylated across gastrointestinal cancers
    Article Snippet: .. Briefly, fragments were amplified for 35 cycles using HotStarTaq (Qiagen) and the purified samples were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit in an AB Prism 3730 sequencer (Life Technologies). .. The electroferograms were analyzed manually using Sequencing Analysis 5.2 (Life Technologies).

    Article Title: Population Genetic Structure of the Staphylococcus intermedius Group: Insights into agr Diversification and the Emergence of Methicillin-Resistant Strains ▿ Diversification and the Emergence of Methicillin-Resistant Strains ▿ †
    Article Snippet: .. Sequencing reactions were carried out using 5 μl purified PCR-amplified DNA (approximately 50 to 90 ng) plus 1 μl sequencing primer (3.2 pmol/μl) with the BigDye Terminator v3.0 Ready Reaction cycle sequencing kit (Applied Biosystems) according to the manufacturer's instructions. ..

    Sequencing:

    Article Title: Association of rheumatic fever rheumatic heart disease with plausible early late-stage disease markers
    Article Snippet: .. The purified products were processed for sequencing reactions in ABI PRISM® dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing kit (Applied Biosystems, USA) following manufacturer's instructions. .. Finally, the sequencing was carried out in 3130×l genetic analyzer of Applied Biosystems.

    Article Title: Epigenetic regulation of MDR1 gene through post-translational histone modifications in prostate cancer
    Article Snippet: .. The purified products were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) in an ABI PRISMTM 310 Genetic Analyzer (Applied Biosystems). ..

    Article Title: Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W]Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W] [OA]
    Article Snippet: .. Sequencing of purified DNA was performed using the Big-Dye Terminator v3.0 ready reaction cycle sequencing kit (Applied Biosystems) and an ABI 3100 automated DNA sequencer (Applied Biosystems). .. PCR conditions for previously available markers were obtained from The Arabidopsis Information Resource, and restriction enzymes used for each marker are listed in Supplemental Table S2.

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: .. The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310. .. YWHAE–FAM22A –FLAG fusion cDNA containing BamHI ( YWHAE end) and EcoRI (FLAG end) restriction sites was synthesized (GenScript) based on the sequences of the fusion transcript present in ESS1 and cloned in pUC57 vector.

    Article Title: Genetic Characterization of Legionella pneumophila Isolated from a Common Watershed in Comunidad Valenciana, Spain
    Article Snippet: .. DNA Sequencing Purified PCR products were subjected to Sanger sequencing using BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) and analyzed in an ABI PRISM 3700 sequencer (Applied Biosystems, Foster City, CA). .. The oligonucleotides used for sequencing were the same used in the amplification reaction except for mompS .

    Article Title: Cloning and expression of a novel catechol-O-methyltransferase in common marmosets
    Article Snippet: .. The sequence verification of insert was carried out using an ABI PRISM BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) with an ABI PRISM 3730 DNA Analyzer (Applied Biosystems). ..

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation. ..

    Article Title: A novel mutation in the WFS1 gene identified in a Taiwanese family with low-frequency hearing impairment
    Article Snippet: .. Direct sequencing A 10-μL sequencing reaction was performed with 100 ng of the purified PCR product as a template, 30 pmol of each of the sequencing primers, and 1 μL of the BigDye Terminator v3.0 Sequencing Ready Reaction Cycle sequencing kit (Applied Biosystems). .. After completion of the reaction, the mixture was purified by gel filtration on a Sephadex G-50 column (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

    Article Title: The novel colorectal cancer biomarkers CDO1, ZSCAN18 and ZNF331 are frequently methylated across gastrointestinal cancers
    Article Snippet: .. Briefly, fragments were amplified for 35 cycles using HotStarTaq (Qiagen) and the purified samples were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit in an AB Prism 3730 sequencer (Life Technologies). .. The electroferograms were analyzed manually using Sequencing Analysis 5.2 (Life Technologies).

    Article Title: Population Genetic Structure of the Staphylococcus intermedius Group: Insights into agr Diversification and the Emergence of Methicillin-Resistant Strains ▿ Diversification and the Emergence of Methicillin-Resistant Strains ▿ †
    Article Snippet: .. Sequencing reactions were carried out using 5 μl purified PCR-amplified DNA (approximately 50 to 90 ng) plus 1 μl sequencing primer (3.2 pmol/μl) with the BigDye Terminator v3.0 Ready Reaction cycle sequencing kit (Applied Biosystems) according to the manufacturer's instructions. ..

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: Paragraph title: Isolation and sequencing of M. domestica IGF2 ... Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    Gel Extraction:

    Article Title: Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W]Quantitative Genetic Analysis of Thermal Dissipation in Arabidopsis 1 [W] [OA]
    Article Snippet: For PCR from genomic DNA, primers were designed using the web-based Primer3 program , and PCR products were separated on agarose gels and then purified using a gel extraction kit (Qiagen). .. Sequencing of purified DNA was performed using the Big-Dye Terminator v3.0 ready reaction cycle sequencing kit (Applied Biosystems) and an ABI 3100 automated DNA sequencer (Applied Biosystems).

    Plasmid Preparation:

    Article Title: Cloning and expression of a novel catechol-O-methyltransferase in common marmosets
    Article Snippet: Purified PCR products were cloned into pGEM-T easy vectors using a pGEM-T Easy Vector System (Promega, Madison, WI, U.S.A.). .. The sequence verification of insert was carried out using an ABI PRISM BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) with an ABI PRISM 3730 DNA Analyzer (Applied Biosystems).

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: BAC DNA was prepared using a QIAGEN™ Plasmid Midi Kit following manufacturer's instructions. .. Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    Software:

    Article Title: Association of rheumatic fever rheumatic heart disease with plausible early late-stage disease markers
    Article Snippet: The purified products were processed for sequencing reactions in ABI PRISM® dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing kit (Applied Biosystems, USA) following manufacturer's instructions. .. The sequence of the amplified product was analyzed using Multialign software ( http://multalin.toulouse.inra.fr/multalin/ ).

    Agarose Gel Electrophoresis:

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: PCR was performed with PCR SuperMix (11306; Invitrogen) according to the manufacturer's protocol with the following cycling conditions: 1 cycle at 94 °C for 2 min followed by 30 cycles of 94 °C for 0.5 min, 55 °C for 0.5 min, 68 °C for 2 min, and a final extension of 68 °C for 5 min. PCR products were evaluated on a 1% agarose gel alongside 1 Kb Plus DNA Ladder (Invitrogen) visualized with ethidium bromide staining. .. The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310.

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: The PCR products were visualised by means of electrophoresis in 1% agarose gel previously stained with ethidium bromide before UV exposure to verify amplification. .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation.

    Chromosome Walking:

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: Sequencing was accomplished by primer walking using BigDye Chemistry (Applied Biosystems), according to the manufacture's protocol. .. Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    Concentration Assay:

    Article Title: Association of rheumatic fever rheumatic heart disease with plausible early late-stage disease markers
    Article Snippet: Cytokine analysis : Serum concentration of interleukin-2 (IL-2), IL-4, IL-6, IL-10, tumour necrosis factor-alpha (TNF-α), interferon gamma (IFN-γ) and IL-12/IL-23p40 was estimated by Human Th1/Th2 Cytokine Kit and Human IL-12/IL-23p40 flex set (BD™ Cytometric Bead Array) following the manufacturer's instructions. .. The purified products were processed for sequencing reactions in ABI PRISM® dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing kit (Applied Biosystems, USA) following manufacturer's instructions.

    Article Title: Species Delimitation in the Genus Moschus (Ruminantia: Moschidae) and Its High-Plateau Origin
    Article Snippet: Polymerase chain reaction (PCR) was performed using a reaction mixture (25 μL) containing 1 μL genomic DNA (concentration 10–50 ng/μL), 2.5 μL 10×buffer, 1 μL of 2.5 mM MgSO4 , 2 μL of 2 mM dNTPs, 2.5 U Taq polymerase (Meridian Bioscience, Singapore) and 0.3 mM of each of the primers. .. The amplification protocol included an initial denaturation step of 95°C for 5 min; this was followed by 32 cycles of denaturation at 95°C for 30 s, primer annealing at 55°C for 30 s, and an extension at 72°C for 80 s, with a final extension at 72°C for 10 min. PCR products were purified using an EasyPure PCR Purification Kit (TransGene), and sequenced using previous primers and the BigDye Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems) following the manufacturer’s instructions on an ABI Prism 3730 automated sequencer.

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: .. Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL. .. Detection of noncoding exons The 5' untranslated exon sequence was captured by RACE using the BD SMART™ RACE cDNA Amplification Kit.

    BAC Assay:

    Article Title: Genomic imprinting of IGF2 in marsupials is methylation dependent
    Article Snippet: BAC DNA was prepared using a QIAGEN™ Plasmid Midi Kit following manufacturer's instructions. .. Regions of repeats or secondary structure were subcloned after Pst I digestion into pBluescript® KS+, then sequenced using 400 ng DNA, 2.3 μL of 5× buffer, 5% DMSO (final concentration), 1 M Betaine (final concentration), 2 μL BigDye Terminator v3.1, dGTP BigDye Terminator v3.0 (Applied Biosystems), 3.2 picomoles primer, and MilliQ water to 20 μL.

    Staining:

    Article Title: Epigenetic regulation of MDR1 gene through post-translational histone modifications in prostate cancer
    Article Snippet: PCR products were loaded onto a nondenaturing 2% agarose gels, stained with ethidium bromide and visualized under an ultraviolet transilluminator. .. The purified products were sequenced using the dGTP BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) in an ABI PRISMTM 310 Genetic Analyzer (Applied Biosystems).

    Article Title: 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma
    Article Snippet: PCR was performed with PCR SuperMix (11306; Invitrogen) according to the manufacturer's protocol with the following cycling conditions: 1 cycle at 94 °C for 2 min followed by 30 cycles of 94 °C for 0.5 min, 55 °C for 0.5 min, 68 °C for 2 min, and a final extension of 68 °C for 5 min. PCR products were evaluated on a 1% agarose gel alongside 1 Kb Plus DNA Ladder (Invitrogen) visualized with ethidium bromide staining. .. The PCR amplicon bands were excised from the gel, purified with a Qiagen Gel Purification Kit, and sequenced with BigDye Terminator v3.0 Ready Reaction Cycle Sequencing (Applied Biosystems) on an ABI PRISM 310.

    Article Title: Genetic Diversity and Population Structure of Busseola segeta Bowden (Lepidoptera; Noctuidae): A Case Study of Host Use Diversification in Guineo-Congolian Rainforest Relic Area, Kenya
    Article Snippet: The PCR products were visualised by means of electrophoresis in 1% agarose gel previously stained with ethidium bromide before UV exposure to verify amplification. .. DNA sequencing reactions were performed using the ABI PRISM® BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems), cleaned using ethanol/EDTA precipitation.

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    Thermo Fisher abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit
    Abi Prism Bigdye Terminator V3 0 Ready Reaction Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 22 article reviews
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    abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit - by Bioz Stars, 2020-04
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