abi prism bigdye terminator cycle sequencing ready reaction kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    Thermo Fisher abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing ready reaction kit/product/Thermo Fisher
    Average 98 stars, based on 393 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator cycle sequencing ready reaction kit - by Bioz Stars, 2020-04
    98/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5?-ACGGA(N11/9)-3?
    Article Snippet: .. The vector-insert junctions of the resulting clones were sequenced using the ABI Prism 310 automated sequencer with ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit and then analyzed using ABI Sequencing Analysis 3.4.1 software (Applied Biosystems, Foster City, CA) and Hitachi DNASIS 2.5 software (Hitachi Software Engineering Co., Yokohamie, Japan). ..

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: A set of PCR products was cloned with the pGEM-T Easy Vector System Kit (Promega, USA) and 5–8 colonies for each cloned fragment were amplified with M13 universal primers and sequenced. .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia).

    Amplification:

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: .. After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United Kingdom
    Article Snippet: Paragraph title: PCR amplification and sequencing. ... The nucleotide sequence was determined by the dideoxynucleotide method ( ) with purified PCR products using (i) the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA polymerase FS (PE Applied Biosystems), analyzing the products on a model 377 ABI DNA sequencer (PE Applied Biosystems), or (ii) the Dye Terminator Cycle Sequencing kit (Beckman Coulter), analyzing the products on a CEQ 2000 DNA Analysis System (Beckman Coulter).

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: To verify the family relationship between the Border Collies, a 261-bp mitochondrial D-loop region was amplified using the primers L15910 and H16498 as previously described [ ]. .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared.

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: The single-stranded cDNA product was then used as the template for PCR amplification with oligonucleotides 197 and 177 (Table ). .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Article Title: Complete genome of a European hepatitis C virus subtype 1g isolate: phylogenetic and genetic analyses
    Article Snippet: All PCRs were performed in a GeneAmp® PCR system 2700 (Applied Biosystems) thermal cycler with the following profile: 95°C for 2 min, then 35 cycles at 95°C for 30 sec, 50–65°C (depending on the primers used) for 30 sec and 72°C for 3 min, and a final extension at 72°C for 10 min. Amplified products were purified with High Pure PCR Products Purification Kit (Roche). .. These purified DNAs were sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit v 3.1 in a 3700 automated sequencer (Applied Biosystems).

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: A set of PCR products was cloned with the pGEM-T Easy Vector System Kit (Promega, USA) and 5–8 colonies for each cloned fragment were amplified with M13 universal primers and sequenced. .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia).

    Article Title: Islands beneath islands: phylogeography of a groundwater amphipod crustacean in the Balearic archipelago
    Article Snippet: PCR was performed on a PTC-100 thermocycler (MJ Research) using a reaction volume of 25 μl and amplification conditions consisted of one cycle at 94°C for 2 min and 40 cycles of 94°C for 30 s, 47-55°C for 30 s and 72°C for 1 min, followed by a final incubation step at 72°C for 10 min. Amplified products were purified with Invitek columns (Invitek GMBH, Berlin, Germany), according to the manufacturer's instructions. .. The fragments were sequenced in both directions using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit v. 2.0 and electrophoresed and detected on an ABI 3100 automated sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
    Article Snippet: .. The P and G types were determined by the size of the amplicons and the genotyping of noroviruses was performed by direct sequencing of the PCR products with the same primers used for amplification by using an ABI Prism BigDye Terminator cycle sequencing ready reaction kit and a 3100 DNA sequencing system (both from Applera Corporation, Forster City, CA). .. Sequence analysis was conducted using Fasta software, version 3, available from the European Bioinformatics Institute.

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer. .. The unknown DNA flanking regions were amplified with primers coupled to biotin at the 5′ end and degenerate flanking primers (Table ) in first-step PCR.

    Lambda DNA Preparation:

    Article Title: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5?-ACGGA(N11/9)-3?
    Article Snippet: The putative Tsp GWI cognate site was further investigated through the digestion of pBR322 DNA and lambda DNA with Tsp GWI, followed by repair of the Tsp GWI restriction fragment termini with T4 DNA polymerase/dNTPs (3) and by cloning the resulting restriction fragments into the Sma I site of a modified pTZ18u vector (4). .. The vector-insert junctions of the resulting clones were sequenced using the ABI Prism 310 automated sequencer with ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit and then analyzed using ABI Sequencing Analysis 3.4.1 software (Applied Biosystems, Foster City, CA) and Hitachi DNASIS 2.5 software (Hitachi Software Engineering Co., Yokohamie, Japan).

    Construct:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Reverse transcription (RT)-PCR amplification and sequencing were used to confirm the presence of intended mutations in viable virus recovered following transfection with transcripts from mutated constructs and to examine potential genomic rearrangements produced by homologous recombination between the two SGPs in dsRobo402/GFP constructs. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia). .. A phylogenetic network among ancient and modern A10 haplotypes was constructed by using the Network program v. 4.6.1.1. ( www.fluxus-enginering.com ).

    Electrophoresis:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. Additionally, RT-PCR was performed with virion RNA extracted from the medium from plates transfected with transcripts from dsRobo402/GFP constructs; the sizes of the amplification products were examined by electrophoresis in 1% agarose gels and visualization by ethidium bromide staining.

    Incubation:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. Following 20 min of incubation at 35°C, 20 μCi of 32 P-orthophosphate (ICN, Costa Mesa, Calif.) was added.

    Article Title: Islands beneath islands: phylogeography of a groundwater amphipod crustacean in the Balearic archipelago
    Article Snippet: PCR was performed on a PTC-100 thermocycler (MJ Research) using a reaction volume of 25 μl and amplification conditions consisted of one cycle at 94°C for 2 min and 40 cycles of 94°C for 30 s, 47-55°C for 30 s and 72°C for 1 min, followed by a final incubation step at 72°C for 10 min. Amplified products were purified with Invitek columns (Invitek GMBH, Berlin, Germany), according to the manufacturer's instructions. .. The fragments were sequenced in both directions using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit v. 2.0 and electrophoresed and detected on an ABI 3100 automated sequencer (Applied Biosystems, Foster City, CA, USA).

    Modification:

    Article Title: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5?-ACGGA(N11/9)-3?
    Article Snippet: The putative Tsp GWI cognate site was further investigated through the digestion of pBR322 DNA and lambda DNA with Tsp GWI, followed by repair of the Tsp GWI restriction fragment termini with T4 DNA polymerase/dNTPs (3) and by cloning the resulting restriction fragments into the Sma I site of a modified pTZ18u vector (4). .. The vector-insert junctions of the resulting clones were sequenced using the ABI Prism 310 automated sequencer with ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit and then analyzed using ABI Sequencing Analysis 3.4.1 software (Applied Biosystems, Foster City, CA) and Hitachi DNASIS 2.5 software (Hitachi Software Engineering Co., Yokohamie, Japan).

    Derivative Assay:

    Article Title: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5?-ACGGA(N11/9)-3?
    Article Snippet: The vector-insert junctions of the resulting clones were sequenced using the ABI Prism 310 automated sequencer with ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit and then analyzed using ABI Sequencing Analysis 3.4.1 software (Applied Biosystems, Foster City, CA) and Hitachi DNASIS 2.5 software (Hitachi Software Engineering Co., Yokohamie, Japan). .. Twenty-five junctions derived from bacteriophage lambda cloned restriction fragments are exemplified in Table , where eight junctions are for the top strand of the 5′-ACGGA-3′ and 17 for the bottom strand of the 5′-TCCGT-3′ reverse complement orientation.

    Transfection:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: One-tenth milliliter of medium from transfection plates maintained with liquid medium or one-fifth of the agar plug fluid from a plaque pick was used to infect a 35-mm2 plate of cells. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Infection:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. For analysis of RNA synthesis in cells transfected or infected with dsRobo402/GFP constructs, after the initial appearance of CPE, the culture fluid was removed, the cells were washed once with 1 ml of phosphate-free D-MEM (Gibco/BRL), and 1 ml of phosphate-free D-MEM containing 1% dialyzed FBS (Gibco/BRL) and 1 μg of actinomycin D/ml was added.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: The total RNA was reverse transcribed into first-strand cDNA using a random hexamer primer and the Superscript first-strand synthesis system of the RT-PCR kit (Invitrogen, USA). .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared.

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Reverse transcription (RT)-PCR amplification and sequencing were used to confirm the presence of intended mutations in viable virus recovered following transfection with transcripts from mutated constructs and to examine potential genomic rearrangements produced by homologous recombination between the two SGPs in dsRobo402/GFP constructs. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Article Title: Complete genome of a European hepatitis C virus subtype 1g isolate: phylogenetic and genetic analyses
    Article Snippet: Paragraph title: Viral purification, RT-PCR and sequencing ... These purified DNAs were sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit v 3.1 in a 3700 automated sequencer (Applied Biosystems).

    Article Title: Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
    Article Snippet: RT-PCRs were performed using a Qiagen One Step RT-PCR kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. The P and G types were determined by the size of the amplicons and the genotyping of noroviruses was performed by direct sequencing of the PCR products with the same primers used for amplification by using an ABI Prism BigDye Terminator cycle sequencing ready reaction kit and a 3100 DNA sequencing system (both from Applera Corporation, Forster City, CA).

    DNA Sequencing:

    Article Title: Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
    Article Snippet: .. The P and G types were determined by the size of the amplicons and the genotyping of noroviruses was performed by direct sequencing of the PCR products with the same primers used for amplification by using an ABI Prism BigDye Terminator cycle sequencing ready reaction kit and a 3100 DNA sequencing system (both from Applera Corporation, Forster City, CA). .. Sequence analysis was conducted using Fasta software, version 3, available from the European Bioinformatics Institute.

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: Paragraph title: DNA sequencing. ... The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer.

    Polymerase Chain Reaction:

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: .. After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United Kingdom
    Article Snippet: .. The nucleotide sequence was determined by the dideoxynucleotide method ( ) with purified PCR products using (i) the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA polymerase FS (PE Applied Biosystems), analyzing the products on a model 377 ABI DNA sequencer (PE Applied Biosystems), or (ii) the Dye Terminator Cycle Sequencing kit (Beckman Coulter), analyzing the products on a CEQ 2000 DNA Analysis System (Beckman Coulter). .. Sequence analyses were performed using the software package GeneBase (Applied Maths, Kortrijk, Belgium).

    Article Title: Characterization of ‘Candidatus Syngnamydia salmonis’ (Chlamydiales, Simkaniaceae), a bacterium associated with epitheliocystis in Atlantic salmon (Salmo salar L.)
    Article Snippet: .. Sequencing and sequence analysis Sequencing of purified PCR products and plasmids was done using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit, v3.1 (Applied Biosystems, Perkin-Elmer) according to producer’s recommendations. ..

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared. .. We designed primers to amplify the coding sequence of the ABCB1 gene; these were based on the wild-type Beagle ABCB1 gene sequence (GenBank accession number NM_001003215).

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: The single-stranded cDNA product was then used as the template for PCR amplification with oligonucleotides 197 and 177 (Table ). .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Article Title: Complete genome of a European hepatitis C virus subtype 1g isolate: phylogenetic and genetic analyses
    Article Snippet: All PCRs were performed in a GeneAmp® PCR system 2700 (Applied Biosystems) thermal cycler with the following profile: 95°C for 2 min, then 35 cycles at 95°C for 30 sec, 50–65°C (depending on the primers used) for 30 sec and 72°C for 3 min, and a final extension at 72°C for 10 min. Amplified products were purified with High Pure PCR Products Purification Kit (Roche). .. These purified DNAs were sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit v 3.1 in a 3700 automated sequencer (Applied Biosystems).

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: The other PCR-fragments were sequenced directly. .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia).

    Article Title: Islands beneath islands: phylogeography of a groundwater amphipod crustacean in the Balearic archipelago
    Article Snippet: PCR was performed on a PTC-100 thermocycler (MJ Research) using a reaction volume of 25 μl and amplification conditions consisted of one cycle at 94°C for 2 min and 40 cycles of 94°C for 30 s, 47-55°C for 30 s and 72°C for 1 min, followed by a final incubation step at 72°C for 10 min. Amplified products were purified with Invitek columns (Invitek GMBH, Berlin, Germany), according to the manufacturer's instructions. .. The fragments were sequenced in both directions using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit v. 2.0 and electrophoresed and detected on an ABI 3100 automated sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: "Mid-G" Region Sequences of the Glycoprotein Gene of Austrian Infectious Hematopoietic Necrosis Virus Isolates Form Two Lineages within European Isolates and Are Distinct from American and Asian Lineages ▿
    Article Snippet: .. An ABI Prism BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems, United Kingdom) diluted in ABI Prism BigDye 5× sequencing buffer (Applied Biosystems) at a ratio of 2 to 3 was used for the sequencing PCR. .. The final primer concentration used for this reaction was 0.2 μM.

    Article Title: Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
    Article Snippet: .. The P and G types were determined by the size of the amplicons and the genotyping of noroviruses was performed by direct sequencing of the PCR products with the same primers used for amplification by using an ABI Prism BigDye Terminator cycle sequencing ready reaction kit and a 3100 DNA sequencing system (both from Applera Corporation, Forster City, CA). .. Sequence analysis was conducted using Fasta software, version 3, available from the European Bioinformatics Institute.

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: .. The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer. .. Unknown flanking DNA fragments were isolated by a two-step PCR method (A.

    Nucleic Acid Electrophoresis:

    Article Title: Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
    Article Snippet: All PCR products were examined by gel electrophoresis in 2% agarose gels containing 10 μg/ml ethidium bromide, and amplicons were viewed with UV light. .. The P and G types were determined by the size of the amplicons and the genotyping of noroviruses was performed by direct sequencing of the PCR products with the same primers used for amplification by using an ABI Prism BigDye Terminator cycle sequencing ready reaction kit and a 3100 DNA sequencing system (both from Applera Corporation, Forster City, CA).

    Isolation:

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: Paragraph title: Isolation and identification of strain ... After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Islands beneath islands: phylogeography of a groundwater amphipod crustacean in the Balearic archipelago
    Article Snippet: Sequencing Genomic DNA was isolated from whole specimens using the DNeasy Tissue Kit (Qiagen, Hilden, Germany), according to the manufacturer's recommendations. .. The fragments were sequenced in both directions using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit v. 2.0 and electrophoresed and detected on an ABI 3100 automated sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer. .. Unknown flanking DNA fragments were isolated by a two-step PCR method (A.

    Size-exclusion Chromatography:

    Article Title: Complete genome of a European hepatitis C virus subtype 1g isolate: phylogenetic and genetic analyses
    Article Snippet: All PCRs were performed in a GeneAmp® PCR system 2700 (Applied Biosystems) thermal cycler with the following profile: 95°C for 2 min, then 35 cycles at 95°C for 30 sec, 50–65°C (depending on the primers used) for 30 sec and 72°C for 3 min, and a final extension at 72°C for 10 min. Amplified products were purified with High Pure PCR Products Purification Kit (Roche). .. These purified DNAs were sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit v 3.1 in a 3700 automated sequencer (Applied Biosystems).

    Purification:

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: .. After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United Kingdom
    Article Snippet: .. The nucleotide sequence was determined by the dideoxynucleotide method ( ) with purified PCR products using (i) the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA polymerase FS (PE Applied Biosystems), analyzing the products on a model 377 ABI DNA sequencer (PE Applied Biosystems), or (ii) the Dye Terminator Cycle Sequencing kit (Beckman Coulter), analyzing the products on a CEQ 2000 DNA Analysis System (Beckman Coulter). .. Sequence analyses were performed using the software package GeneBase (Applied Maths, Kortrijk, Belgium).

    Article Title: Characterization of ‘Candidatus Syngnamydia salmonis’ (Chlamydiales, Simkaniaceae), a bacterium associated with epitheliocystis in Atlantic salmon (Salmo salar L.)
    Article Snippet: .. Sequencing and sequence analysis Sequencing of purified PCR products and plasmids was done using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit, v3.1 (Applied Biosystems, Perkin-Elmer) according to producer’s recommendations. ..

    Article Title: Complete genome of a European hepatitis C virus subtype 1g isolate: phylogenetic and genetic analyses
    Article Snippet: .. These purified DNAs were sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit v 3.1 in a 3700 automated sequencer (Applied Biosystems). ..

    Article Title: Islands beneath islands: phylogeography of a groundwater amphipod crustacean in the Balearic archipelago
    Article Snippet: PCR was performed on a PTC-100 thermocycler (MJ Research) using a reaction volume of 25 μl and amplification conditions consisted of one cycle at 94°C for 2 min and 40 cycles of 94°C for 30 s, 47-55°C for 30 s and 72°C for 1 min, followed by a final incubation step at 72°C for 10 min. Amplified products were purified with Invitek columns (Invitek GMBH, Berlin, Germany), according to the manufacturer's instructions. .. The fragments were sequenced in both directions using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit v. 2.0 and electrophoresed and detected on an ABI 3100 automated sequencer (Applied Biosystems, Foster City, CA, USA).

    Sequencing:

    Article Title: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5?-ACGGA(N11/9)-3?
    Article Snippet: .. The vector-insert junctions of the resulting clones were sequenced using the ABI Prism 310 automated sequencer with ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit and then analyzed using ABI Sequencing Analysis 3.4.1 software (Applied Biosystems, Foster City, CA) and Hitachi DNASIS 2.5 software (Hitachi Software Engineering Co., Yokohamie, Japan). ..

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: .. After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United Kingdom
    Article Snippet: .. The nucleotide sequence was determined by the dideoxynucleotide method ( ) with purified PCR products using (i) the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA polymerase FS (PE Applied Biosystems), analyzing the products on a model 377 ABI DNA sequencer (PE Applied Biosystems), or (ii) the Dye Terminator Cycle Sequencing kit (Beckman Coulter), analyzing the products on a CEQ 2000 DNA Analysis System (Beckman Coulter). .. Sequence analyses were performed using the software package GeneBase (Applied Maths, Kortrijk, Belgium).

    Article Title: Characterization of ‘Candidatus Syngnamydia salmonis’ (Chlamydiales, Simkaniaceae), a bacterium associated with epitheliocystis in Atlantic salmon (Salmo salar L.)
    Article Snippet: .. Sequencing and sequence analysis Sequencing of purified PCR products and plasmids was done using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit, v3.1 (Applied Biosystems, Perkin-Elmer) according to producer’s recommendations. ..

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared. .. We designed primers to amplify the coding sequence of the ABCB1 gene; these were based on the wild-type Beagle ABCB1 gene sequence (GenBank accession number NM_001003215).

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. Additionally, RT-PCR was performed with virion RNA extracted from the medium from plates transfected with transcripts from dsRobo402/GFP constructs; the sizes of the amplification products were examined by electrophoresis in 1% agarose gels and visualization by ethidium bromide staining.

    Article Title: Complete genome of a European hepatitis C virus subtype 1g isolate: phylogenetic and genetic analyses
    Article Snippet: .. These purified DNAs were sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit v 3.1 in a 3700 automated sequencer (Applied Biosystems). ..

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia). .. Sequences were aligned with the revised Cambridge Reference Sequence of human mtDNA (rCRS) [ ] by using BioEdit software version 7.0.5.

    Article Title: Islands beneath islands: phylogeography of a groundwater amphipod crustacean in the Balearic archipelago
    Article Snippet: .. The fragments were sequenced in both directions using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit v. 2.0 and electrophoresed and detected on an ABI 3100 automated sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: "Mid-G" Region Sequences of the Glycoprotein Gene of Austrian Infectious Hematopoietic Necrosis Virus Isolates Form Two Lineages within European Isolates and Are Distinct from American and Asian Lineages ▿
    Article Snippet: .. An ABI Prism BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems, United Kingdom) diluted in ABI Prism BigDye 5× sequencing buffer (Applied Biosystems) at a ratio of 2 to 3 was used for the sequencing PCR. .. The final primer concentration used for this reaction was 0.2 μM.

    Article Title: Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
    Article Snippet: .. The P and G types were determined by the size of the amplicons and the genotyping of noroviruses was performed by direct sequencing of the PCR products with the same primers used for amplification by using an ABI Prism BigDye Terminator cycle sequencing ready reaction kit and a 3100 DNA sequencing system (both from Applera Corporation, Forster City, CA). .. Sequence analysis was conducted using Fasta software, version 3, available from the European Bioinformatics Institute.

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: .. The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer. .. Unknown flanking DNA fragments were isolated by a two-step PCR method (A.

    Plasmid Preparation:

    Article Title: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5?-ACGGA(N11/9)-3?
    Article Snippet: .. The vector-insert junctions of the resulting clones were sequenced using the ABI Prism 310 automated sequencer with ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit and then analyzed using ABI Sequencing Analysis 3.4.1 software (Applied Biosystems, Foster City, CA) and Hitachi DNASIS 2.5 software (Hitachi Software Engineering Co., Yokohamie, Japan). ..

    Article Title: Characterization of ‘Candidatus Syngnamydia salmonis’ (Chlamydiales, Simkaniaceae), a bacterium associated with epitheliocystis in Atlantic salmon (Salmo salar L.)
    Article Snippet: Sequencing and sequence analysis Sequencing of purified PCR products and plasmids was done using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit, v3.1 (Applied Biosystems, Perkin-Elmer) according to producer’s recommendations. .. An alignment of 56 16s rRNA gene sequences from the phylum Chlamydiae , retrieved from the GenBank or obtained from the present study, was made using Vector NTI 9.0 software.

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: A set of PCR products was cloned with the pGEM-T Easy Vector System Kit (Promega, USA) and 5–8 colonies for each cloned fragment were amplified with M13 universal primers and sequenced. .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia).

    Software:

    Article Title: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5?-ACGGA(N11/9)-3?
    Article Snippet: .. The vector-insert junctions of the resulting clones were sequenced using the ABI Prism 310 automated sequencer with ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit and then analyzed using ABI Sequencing Analysis 3.4.1 software (Applied Biosystems, Foster City, CA) and Hitachi DNASIS 2.5 software (Hitachi Software Engineering Co., Yokohamie, Japan). ..

    Article Title: Characterization of ‘Candidatus Syngnamydia salmonis’ (Chlamydiales, Simkaniaceae), a bacterium associated with epitheliocystis in Atlantic salmon (Salmo salar L.)
    Article Snippet: Sequencing and sequence analysis Sequencing of purified PCR products and plasmids was done using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit, v3.1 (Applied Biosystems, Perkin-Elmer) according to producer’s recommendations. .. An alignment of 56 16s rRNA gene sequences from the phylum Chlamydiae , retrieved from the GenBank or obtained from the present study, was made using Vector NTI 9.0 software.

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared. .. The conditions of the designed primers were verified using DNAMAN software v.4.16 (Lynnon, Canada).

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia). .. Sequences were aligned with the revised Cambridge Reference Sequence of human mtDNA (rCRS) [ ] by using BioEdit software version 7.0.5.

    Article Title: Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
    Article Snippet: The P and G types were determined by the size of the amplicons and the genotyping of noroviruses was performed by direct sequencing of the PCR products with the same primers used for amplification by using an ABI Prism BigDye Terminator cycle sequencing ready reaction kit and a 3100 DNA sequencing system (both from Applera Corporation, Forster City, CA). .. Sequence analysis was conducted using Fasta software, version 3, available from the European Bioinformatics Institute.

    Random Hexamer Labeling:

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: The total RNA was reverse transcribed into first-strand cDNA using a random hexamer primer and the Superscript first-strand synthesis system of the RT-PCR kit (Invitrogen, USA). .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared.

    Produced:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Reverse transcription (RT)-PCR amplification and sequencing were used to confirm the presence of intended mutations in viable virus recovered following transfection with transcripts from mutated constructs and to examine potential genomic rearrangements produced by homologous recombination between the two SGPs in dsRobo402/GFP constructs. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Concentration Assay:

    Article Title: "Mid-G" Region Sequences of the Glycoprotein Gene of Austrian Infectious Hematopoietic Necrosis Virus Isolates Form Two Lineages within European Isolates and Are Distinct from American and Asian Lineages ▿
    Article Snippet: An ABI Prism BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems, United Kingdom) diluted in ABI Prism BigDye 5× sequencing buffer (Applied Biosystems) at a ratio of 2 to 3 was used for the sequencing PCR. .. The final primer concentration used for this reaction was 0.2 μM.

    Staining:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. Additionally, RT-PCR was performed with virion RNA extracted from the medium from plates transfected with transcripts from dsRobo402/GFP constructs; the sizes of the amplification products were examined by electrophoresis in 1% agarose gels and visualization by ethidium bromide staining.

    Homologous Recombination:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Reverse transcription (RT)-PCR amplification and sequencing were used to confirm the presence of intended mutations in viable virus recovered following transfection with transcripts from mutated constructs and to examine potential genomic rearrangements produced by homologous recombination between the two SGPs in dsRobo402/GFP constructs. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Thermo Fisher abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing ready reaction kit/product/Thermo Fisher
    Average 98 stars, based on 393 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator cycle sequencing ready reaction kit - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    Image Search Results