abi prism bigdye terminator cycle sequencing ready reaction kit  (Thermo Fisher)


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    Thermo Fisher abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing ready reaction kit/product/Thermo Fisher
    Average 99 stars, based on 393 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator cycle sequencing ready reaction kit - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: A set of PCR products was cloned with the pGEM-T Easy Vector System Kit (Promega, USA) and 5–8 colonies for each cloned fragment were amplified with M13 universal primers and sequenced. .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia).

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, cloning and sequencing ... Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction.

    Amplification:

    Article Title: High prevalence of norovirus in children with sporadic acute gastroenteritis in Manaus, Amazon Region, northern Brazil
    Article Snippet: Molecular detection - Polymerase chain reaction (PCR) amplification was performed in all samples using a pool of primers for the B region (nt: 5093-5305; 213 bp) of the NoV polymerase gene (ORF1): Mon 431/433 and 432/434 to detect GI and GII, respectively ( ). .. DNA sequencing was performed using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit and the ABI Prism 3130xl DNA Sequencer (Applied Biosystems® , Foster City, CA, USA).

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: .. After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United Kingdom
    Article Snippet: Paragraph title: PCR amplification and sequencing. ... The nucleotide sequence was determined by the dideoxynucleotide method ( ) with purified PCR products using (i) the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA polymerase FS (PE Applied Biosystems), analyzing the products on a model 377 ABI DNA sequencer (PE Applied Biosystems), or (ii) the Dye Terminator Cycle Sequencing kit (Beckman Coulter), analyzing the products on a CEQ 2000 DNA Analysis System (Beckman Coulter).

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: To verify the family relationship between the Border Collies, a 261-bp mitochondrial D-loop region was amplified using the primers L15910 and H16498 as previously described [ ]. .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared.

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: The single-stranded cDNA product was then used as the template for PCR amplification with oligonucleotides 197 and 177 (Table ). .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Article Title: Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy
    Article Snippet: PCR amplified HBV DNA was purified by using a QIAgen PCR purification kit (Qiagen Inc, Vanencia, CA, U.S.A.). .. Purified DNA was treated with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, CA, U.S.A.).

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: A set of PCR products was cloned with the pGEM-T Easy Vector System Kit (Promega, USA) and 5–8 colonies for each cloned fragment were amplified with M13 universal primers and sequenced. .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia).

    Article Title: Rapid Identification of Mycobacteria to the Species Level Using INNO-LiPA Mycobacteria, a Reverse Hybridization Assay
    Article Snippet: .. For some isolates, the amplified 16S-23S spacer region generated as described above was purified using a QIAquick PCR purification kit (Qiagen, Chatsworth, Calif.) and sequenced using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit on an ABI PRISM 377 sequencer (Applied Biosystems, Foster City, Calif.). ..

    Article Title: Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
    Article Snippet: DNA extraction and polymerase chain reaction amplification DNA of the Candida strains was extracted from the fresh colonies using the previously described methods [ , ]. .. Sequencing and Sequence Analysis The PCR products of HWP1 gene were purified using QIAquick Purification Kit (Qi-agen, Valencia, CA, USA) and subjected to ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, cloning and sequencing ... Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction.

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer. .. The unknown DNA flanking regions were amplified with primers coupled to biotin at the 5′ end and degenerate flanking primers (Table ) in first-step PCR.

    Article Title: Characterization of Neisseria meningitidis Isolates from Recent Outbreaks in Ethiopia and Comparison with Those Recovered during the Epidemic of 1988 to 1989
    Article Snippet: Briefly, fragments of each of the seven genes abcZ , adk , aroE , gdh , pdhC , pgm , and fumC were amplified by PCR on a boiled bacterial cell suspension. .. Sequencing of the PCR products was performed on both strands using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, Foster City, CA) according to the manufacturer's instructions.

    Filtration:

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction. .. Cycle-sequencing products were purified on 96-well multiscreen filtration plates (Millipore, Zug, Switzerland) to remove unincorporated BigDye terminator and then analyzed on an ABI 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA).

    Random Hexamer Labeling:

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: The total RNA was reverse transcribed into first-strand cDNA using a random hexamer primer and the Superscript first-strand synthesis system of the RT-PCR kit (Invitrogen, USA). .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared.

    Construct:

    Article Title: High prevalence of norovirus in children with sporadic acute gastroenteritis in Manaus, Amazon Region, northern Brazil
    Article Snippet: DNA sequencing was performed using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit and the ABI Prism 3130xl DNA Sequencer (Applied Biosystems® , Foster City, CA, USA). .. Consensual sequences were used to construct a phylogenetic dendrogram by the neighbour-joining method, using a matrix of genetic distances established under the Kimura two-parameter model with 2000 bootstrap replications for branch support using MEGA 6 ( ).

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Reverse transcription (RT)-PCR amplification and sequencing were used to confirm the presence of intended mutations in viable virus recovered following transfection with transcripts from mutated constructs and to examine potential genomic rearrangements produced by homologous recombination between the two SGPs in dsRobo402/GFP constructs. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia). .. A phylogenetic network among ancient and modern A10 haplotypes was constructed by using the Network program v. 4.6.1.1. ( www.fluxus-enginering.com ).

    Electrophoresis:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. Additionally, RT-PCR was performed with virion RNA extracted from the medium from plates transfected with transcripts from dsRobo402/GFP constructs; the sizes of the amplification products were examined by electrophoresis in 1% agarose gels and visualization by ethidium bromide staining.

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: The materials required for electrophoresis included the DNA marker, agarose gel, and Microcon 100 (Millipore Corporation, Nepean, Ontario, Canada) used for subsequent PCR product purification for sequencing. .. The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems.

    Incubation:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. Following 20 min of incubation at 35°C, 20 μCi of 32 P-orthophosphate (ICN, Costa Mesa, Calif.) was added.

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: After addition of 125 ml of LB medium, cells were incubated at 37°C for one hour and then spread on 50 μg/ml Ampicillin-agar plates. .. Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction.

    Transformation Assay:

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: One Shot® MAX Efficiency® DH5α™-T1R Competent Cells (Invitrogen AG, Basel, Switzerland) were used for transformation. .. Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction.

    Transfection:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: One-tenth milliliter of medium from transfection plates maintained with liquid medium or one-fifth of the agar plug fluid from a plaque pick was used to infect a 35-mm2 plate of cells. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Sequencing:

    Article Title: High prevalence of norovirus in children with sporadic acute gastroenteritis in Manaus, Amazon Region, northern Brazil
    Article Snippet: .. DNA sequencing was performed using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit and the ABI Prism 3130xl DNA Sequencer (Applied Biosystems® , Foster City, CA, USA). .. The sequences were edited using the BioEdit Sequence Alignment Editor (v.7.0.9.1) software.

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: .. After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United Kingdom
    Article Snippet: .. The nucleotide sequence was determined by the dideoxynucleotide method ( ) with purified PCR products using (i) the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA polymerase FS (PE Applied Biosystems), analyzing the products on a model 377 ABI DNA sequencer (PE Applied Biosystems), or (ii) the Dye Terminator Cycle Sequencing kit (Beckman Coulter), analyzing the products on a CEQ 2000 DNA Analysis System (Beckman Coulter). .. Sequence analyses were performed using the software package GeneBase (Applied Maths, Kortrijk, Belgium).

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared. .. We designed primers to amplify the coding sequence of the ABCB1 gene; these were based on the wild-type Beagle ABCB1 gene sequence (GenBank accession number NM_001003215).

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. Additionally, RT-PCR was performed with virion RNA extracted from the medium from plates transfected with transcripts from dsRobo402/GFP constructs; the sizes of the amplification products were examined by electrophoresis in 1% agarose gels and visualization by ethidium bromide staining.

    Article Title: Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy
    Article Snippet: .. Purified DNA was treated with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, CA, U.S.A.). .. Sequences were determined using an ABI Prism 377 DNA sequencer (Applied Biosystems) by a bidirectional sequencing method.

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia). .. Sequences were aligned with the revised Cambridge Reference Sequence of human mtDNA (rCRS) [ ] by using BioEdit software version 7.0.5.

    Article Title: Rapid Identification of Mycobacteria to the Species Level Using INNO-LiPA Mycobacteria, a Reverse Hybridization Assay
    Article Snippet: .. For some isolates, the amplified 16S-23S spacer region generated as described above was purified using a QIAquick PCR purification kit (Qiagen, Chatsworth, Calif.) and sequenced using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit on an ABI PRISM 377 sequencer (Applied Biosystems, Foster City, Calif.). ..

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: .. The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems. .. Centricep columns (Princeton Separations, Adelphia, N.J.) were used for purification of sequencing product.

    Article Title: TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation
    Article Snippet: .. DNA sequencing was performed using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) on an Applied Biosystems 3100 automatic DNA sequencer. .. Sequencing of TM4SF10 coding region The human TM4SF10 gene is composed of 3 exons and produces a 4 kb-long mRNA.

    Article Title: Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
    Article Snippet: .. Sequencing and Sequence Analysis The PCR products of HWP1 gene were purified using QIAquick Purification Kit (Qi-agen, Valencia, CA, USA) and subjected to ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA). .. For all isolates, the forward and reverse primers were used for the sequencing of purified gene fragments by an automated DNA sequencer (ABI PrismTM 3730 Genetic Analyzer, Applied Biosystems).

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: .. Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction. .. Cycle-sequencing products were purified on 96-well multiscreen filtration plates (Millipore, Zug, Switzerland) to remove unincorporated BigDye terminator and then analyzed on an ABI 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA).

    Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression
    Article Snippet: .. For cell lines, after being purified, the PCR products were subjected to a cycle sequencing reaction with an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA), and then analysed with an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems) in accordance with the manufacturer's instructions. .. Results Immunohistochemistry for PDGFR-α was performed in 181 invasive ductal breast carcinomas and in the two breast cancer cell lines.

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: .. The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer. .. Unknown flanking DNA fragments were isolated by a two-step PCR method (A.

    Article Title: Characterization of Neisseria meningitidis Isolates from Recent Outbreaks in Ethiopia and Comparison with Those Recovered during the Epidemic of 1988 to 1989
    Article Snippet: .. Sequencing of the PCR products was performed on both strands using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, Foster City, CA) according to the manufacturer's instructions. .. Sequencing reactions were run on an ABI Prism 377 instrument (Applied Biosystems) using 5% Long Ranger gels (FMC Bioproducts, Rockland, ME).

    Transferring:

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems. .. Consumables generally consisted of variable amounts of materials such as latex gloves, loops and syringes, disposable transfer pipettes, pipette tips, and microcentrifuge tubes, among other things.

    Infection:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. For analysis of RNA synthesis in cells transfected or infected with dsRobo402/GFP constructs, after the initial appearance of CPE, the culture fluid was removed, the cells were washed once with 1 ml of phosphate-free D-MEM (Gibco/BRL), and 1 ml of phosphate-free D-MEM containing 1% dialyzed FBS (Gibco/BRL) and 1 μg of actinomycin D/ml was added.

    Generated:

    Article Title: Rapid Identification of Mycobacteria to the Species Level Using INNO-LiPA Mycobacteria, a Reverse Hybridization Assay
    Article Snippet: .. For some isolates, the amplified 16S-23S spacer region generated as described above was purified using a QIAquick PCR purification kit (Qiagen, Chatsworth, Calif.) and sequenced using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit on an ABI PRISM 377 sequencer (Applied Biosystems, Foster City, Calif.). ..

    DNA Sequencing:

    Article Title: High prevalence of norovirus in children with sporadic acute gastroenteritis in Manaus, Amazon Region, northern Brazil
    Article Snippet: .. DNA sequencing was performed using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit and the ABI Prism 3130xl DNA Sequencer (Applied Biosystems® , Foster City, CA, USA). .. The sequences were edited using the BioEdit Sequence Alignment Editor (v.7.0.9.1) software.

    Article Title: TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation
    Article Snippet: .. DNA sequencing was performed using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) on an Applied Biosystems 3100 automatic DNA sequencer. .. Sequencing of TM4SF10 coding region The human TM4SF10 gene is composed of 3 exons and produces a 4 kb-long mRNA.

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: Paragraph title: DNA sequencing. ... The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer.

    Polymerase Chain Reaction:

    Article Title: High prevalence of norovirus in children with sporadic acute gastroenteritis in Manaus, Amazon Region, northern Brazil
    Article Snippet: The amplicons obtained were purified with the commercial kit QIAquick PCR Purification Kit (Qiagen® , Valencia, CA, USA) and quantified using the Low DNA Mass Ladder (Invitrogen®, Carlsbad, CA, USA). .. DNA sequencing was performed using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit and the ABI Prism 3130xl DNA Sequencer (Applied Biosystems® , Foster City, CA, USA).

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: .. After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United Kingdom
    Article Snippet: .. The nucleotide sequence was determined by the dideoxynucleotide method ( ) with purified PCR products using (i) the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA polymerase FS (PE Applied Biosystems), analyzing the products on a model 377 ABI DNA sequencer (PE Applied Biosystems), or (ii) the Dye Terminator Cycle Sequencing kit (Beckman Coulter), analyzing the products on a CEQ 2000 DNA Analysis System (Beckman Coulter). .. Sequence analyses were performed using the software package GeneBase (Applied Maths, Kortrijk, Belgium).

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared. .. We designed primers to amplify the coding sequence of the ABCB1 gene; these were based on the wild-type Beagle ABCB1 gene sequence (GenBank accession number NM_001003215).

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: The single-stranded cDNA product was then used as the template for PCR amplification with oligonucleotides 197 and 177 (Table ). .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Article Title: Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy
    Article Snippet: PCR amplified HBV DNA was purified by using a QIAgen PCR purification kit (Qiagen Inc, Vanencia, CA, U.S.A.). .. Purified DNA was treated with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, CA, U.S.A.).

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: The other PCR-fragments were sequenced directly. .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia).

    Article Title: Rapid Identification of Mycobacteria to the Species Level Using INNO-LiPA Mycobacteria, a Reverse Hybridization Assay
    Article Snippet: .. For some isolates, the amplified 16S-23S spacer region generated as described above was purified using a QIAquick PCR purification kit (Qiagen, Chatsworth, Calif.) and sequenced using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit on an ABI PRISM 377 sequencer (Applied Biosystems, Foster City, Calif.). ..

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: The materials required for electrophoresis included the DNA marker, agarose gel, and Microcon 100 (Millipore Corporation, Nepean, Ontario, Canada) used for subsequent PCR product purification for sequencing. .. The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems.

    Article Title: TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation
    Article Snippet: About 50–100 ng of purified PCR fragment was used in a DNA sequencing reaction using a nested, internal primer. .. DNA sequencing was performed using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) on an Applied Biosystems 3100 automatic DNA sequencer.

    Article Title: Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
    Article Snippet: .. Sequencing and Sequence Analysis The PCR products of HWP1 gene were purified using QIAquick Purification Kit (Qi-agen, Valencia, CA, USA) and subjected to ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA). .. For all isolates, the forward and reverse primers were used for the sequencing of purified gene fragments by an automated DNA sequencer (ABI PrismTM 3730 Genetic Analyzer, Applied Biosystems).

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, cloning and sequencing ... Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction.

    Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression
    Article Snippet: .. For cell lines, after being purified, the PCR products were subjected to a cycle sequencing reaction with an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA), and then analysed with an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems) in accordance with the manufacturer's instructions. .. Results Immunohistochemistry for PDGFR-α was performed in 181 invasive ductal breast carcinomas and in the two breast cancer cell lines.

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: .. The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer. .. Unknown flanking DNA fragments were isolated by a two-step PCR method (A.

    Article Title: Characterization of Neisseria meningitidis Isolates from Recent Outbreaks in Ethiopia and Comparison with Those Recovered during the Epidemic of 1988 to 1989
    Article Snippet: .. Sequencing of the PCR products was performed on both strands using the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, Foster City, CA) according to the manufacturer's instructions. .. Sequencing reactions were run on an ABI Prism 377 instrument (Applied Biosystems) using 5% Long Ranger gels (FMC Bioproducts, Rockland, ME).

    Molecular Weight:

    Article Title: TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation
    Article Snippet: Purified PCR fragments were roughly quantitated by agarose gel electrophoresis using SmartLadder molecular weight marker (EUROGENTEC) as a quantitative reference. .. DNA sequencing was performed using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) on an Applied Biosystems 3100 automatic DNA sequencer.

    DNA Extraction:

    Article Title: Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
    Article Snippet: DNA extraction and polymerase chain reaction amplification DNA of the Candida strains was extracted from the fresh colonies using the previously described methods [ , ]. .. Sequencing and Sequence Analysis The PCR products of HWP1 gene were purified using QIAquick Purification Kit (Qi-agen, Valencia, CA, USA) and subjected to ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, cloning and sequencing ... Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction.

    Nucleic Acid Electrophoresis:

    Article Title: Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy
    Article Snippet: PCR products were stained with ethidium bromide after gel electrophoresis. .. Purified DNA was treated with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, CA, U.S.A.).

    Isolation:

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: Paragraph title: Isolation and identification of strain ... After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Frequency of Disinfectant Resistance Genes and Genetic Linkage with ?-Lactamase Transposon Tn552 among Clinical Staphylococci
    Article Snippet: The nucleotide sequences of PCR products and XL-PCR products were determined by using an ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems) with synthetic oligonucleotide primers on an ABI PRISM 377 automatic sequencer (Applied Biosystems) as recommended by the manufacturer. .. Unknown flanking DNA fragments were isolated by a two-step PCR method (A.

    Size-exclusion Chromatography:

    Article Title: Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy
    Article Snippet: PCR was performed for 30 cycles of denaturation at 94℃ for 30 sec and annealing at 55℃ for 30 sec, which was followed by a final primer extension at 72℃ for 1 min. .. Purified DNA was treated with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, CA, U.S.A.).

    Article Title: TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation
    Article Snippet: After an initial denaturation step (93°C, 45 sec.), 35 cycles were conducted as follows: 93°C, 45 sec.; annealing temp. (see table ), 45 sec.; 72°C, 45 sec. (amplicons Ex1–Ex3) or 1 min. (amplicons 3'UTR F1–F4). .. DNA sequencing was performed using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) on an Applied Biosystems 3100 automatic DNA sequencer.

    Purification:

    Article Title: High prevalence of norovirus in children with sporadic acute gastroenteritis in Manaus, Amazon Region, northern Brazil
    Article Snippet: The amplicons obtained were purified with the commercial kit QIAquick PCR Purification Kit (Qiagen® , Valencia, CA, USA) and quantified using the Low DNA Mass Ladder (Invitrogen®, Carlsbad, CA, USA). .. DNA sequencing was performed using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit and the ABI Prism 3130xl DNA Sequencer (Applied Biosystems® , Foster City, CA, USA).

    Article Title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
    Article Snippet: .. After amplification, the PCR products were purified using an AccuPrep PCR purification kit (BIONEER, Daejeon, Korea) and sequenced with the ABI prism™ Bigdye™ Terminator Cycle sequencing ready reaction kit in an automated ABI 3730XL capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United Kingdom
    Article Snippet: .. The nucleotide sequence was determined by the dideoxynucleotide method ( ) with purified PCR products using (i) the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA polymerase FS (PE Applied Biosystems), analyzing the products on a model 377 ABI DNA sequencer (PE Applied Biosystems), or (ii) the Dye Terminator Cycle Sequencing kit (Beckman Coulter), analyzing the products on a CEQ 2000 DNA Analysis System (Beckman Coulter). .. Sequence analyses were performed using the software package GeneBase (Applied Maths, Kortrijk, Belgium).

    Article Title: Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy
    Article Snippet: .. Purified DNA was treated with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, CA, U.S.A.). .. Sequences were determined using an ABI Prism 377 DNA sequencer (Applied Biosystems) by a bidirectional sequencing method.

    Article Title: Rapid Identification of Mycobacteria to the Species Level Using INNO-LiPA Mycobacteria, a Reverse Hybridization Assay
    Article Snippet: .. For some isolates, the amplified 16S-23S spacer region generated as described above was purified using a QIAquick PCR purification kit (Qiagen, Chatsworth, Calif.) and sequenced using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit on an ABI PRISM 377 sequencer (Applied Biosystems, Foster City, Calif.). ..

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: The materials required for electrophoresis included the DNA marker, agarose gel, and Microcon 100 (Millipore Corporation, Nepean, Ontario, Canada) used for subsequent PCR product purification for sequencing. .. The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems.

    Article Title: TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation
    Article Snippet: About 50–100 ng of purified PCR fragment was used in a DNA sequencing reaction using a nested, internal primer. .. DNA sequencing was performed using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) on an Applied Biosystems 3100 automatic DNA sequencer.

    Article Title: Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
    Article Snippet: Sequencing and Sequence Analysis The PCR products of HWP1 gene were purified using QIAquick Purification Kit (Qi-agen, Valencia, CA, USA) and subjected to ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA). .. For all isolates, the forward and reverse primers were used for the sequencing of purified gene fragments by an automated DNA sequencer (ABI PrismTM 3730 Genetic Analyzer, Applied Biosystems).

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction. .. Cycle-sequencing products were purified on 96-well multiscreen filtration plates (Millipore, Zug, Switzerland) to remove unincorporated BigDye terminator and then analyzed on an ABI 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA).

    Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression
    Article Snippet: .. For cell lines, after being purified, the PCR products were subjected to a cycle sequencing reaction with an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA), and then analysed with an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems) in accordance with the manufacturer's instructions. .. Results Immunohistochemistry for PDGFR-α was performed in 181 invasive ductal breast carcinomas and in the two breast cancer cell lines.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: The total RNA was reverse transcribed into first-strand cDNA using a random hexamer primer and the Superscript first-strand synthesis system of the RT-PCR kit (Invitrogen, USA). .. All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared.

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Reverse transcription (RT)-PCR amplification and sequencing were used to confirm the presence of intended mutations in viable virus recovered following transfection with transcripts from mutated constructs and to examine potential genomic rearrangements produced by homologous recombination between the two SGPs in dsRobo402/GFP constructs. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Staining:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.). .. Additionally, RT-PCR was performed with virion RNA extracted from the medium from plates transfected with transcripts from dsRobo402/GFP constructs; the sizes of the amplification products were examined by electrophoresis in 1% agarose gels and visualization by ethidium bromide staining.

    Article Title: Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy
    Article Snippet: PCR products were stained with ethidium bromide after gel electrophoresis. .. Purified DNA was treated with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, CA, U.S.A.).

    Plasmid Preparation:

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: A set of PCR products was cloned with the pGEM-T Easy Vector System Kit (Promega, USA) and 5–8 colonies for each cloned fragment were amplified with M13 universal primers and sequenced. .. Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia).

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: The plasmid vector pJET was added to the cells, and these were shocked at 42°C for 30 seconds. .. Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction.

    Software:

    Article Title: High prevalence of norovirus in children with sporadic acute gastroenteritis in Manaus, Amazon Region, northern Brazil
    Article Snippet: DNA sequencing was performed using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit and the ABI Prism 3130xl DNA Sequencer (Applied Biosystems® , Foster City, CA, USA). .. The sequences were edited using the BioEdit Sequence Alignment Editor (v.7.0.9.1) software.

    Article Title: Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
    Article Snippet: All PCR products were sequenced using an ABI Prism BigDye terminator cycle sequencing ready reaction kit v.5.1 (PE Applied Biosystems, USA) and the sequences were compared. .. The conditions of the designed primers were verified using DNAMAN software v.4.16 (Lynnon, Canada).

    Article Title: MtDNA Haplogroup A10 Lineages in Bronze Age Samples Suggest That Ancient Autochthonous Human Groups Contributed to the Specificity of the Indigenous West Siberian Population
    Article Snippet: Sequencing reactions were performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA) and were analyzed on an ABI Prism 3130XL Genetic Analyzer (Applied Biosystems, USA) at the SB RAS Genomics Core Facility (Novosibirsk, Russia). .. Sequences were aligned with the revised Cambridge Reference Sequence of human mtDNA (rCRS) [ ] by using BioEdit software version 7.0.5.

    Article Title: Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
    Article Snippet: Primer des-ignning was carried out using the AlleleID software, version 7.0 (Premier Biosoft International, Palo Alto, CA, USA). .. Sequencing and Sequence Analysis The PCR products of HWP1 gene were purified using QIAquick Purification Kit (Qi-agen, Valencia, CA, USA) and subjected to ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Polyploid evolution and Pleistocene glacial cycles: A case study from the alpine primrose Primula marginata (Primulaceae)
    Article Snippet: Cycle sequencing reactions were performed with the ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Rotkreuz, Switzerland), using 1 μl of BigDye terminator per reaction. .. The obtained sequences were edited and assembled using Sequencher 4.2 software (Gene Codes Corp., Ann Arbor, Michigan, USA).

    Agarose Gel Electrophoresis:

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: The materials required for electrophoresis included the DNA marker, agarose gel, and Microcon 100 (Millipore Corporation, Nepean, Ontario, Canada) used for subsequent PCR product purification for sequencing. .. The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems.

    Article Title: TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation
    Article Snippet: Purified PCR fragments were roughly quantitated by agarose gel electrophoresis using SmartLadder molecular weight marker (EUROGENTEC) as a quantitative reference. .. DNA sequencing was performed using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) on an Applied Biosystems 3100 automatic DNA sequencer.

    Quantitation Assay:

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: The materials required for crude lysate preparation included consumables associated with the Mini-Beadbeater as well as the PicoGreen dsDNA quantitation kit (Molecular Probes, Inc., Eugene, Oreg.) for quantitation of the lysate. .. The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems.

    Produced:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Reverse transcription (RT)-PCR amplification and sequencing were used to confirm the presence of intended mutations in viable virus recovered following transfection with transcripts from mutated constructs and to examine potential genomic rearrangements produced by homologous recombination between the two SGPs in dsRobo402/GFP constructs. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

    Marker:

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: The materials required for electrophoresis included the DNA marker, agarose gel, and Microcon 100 (Millipore Corporation, Nepean, Ontario, Canada) used for subsequent PCR product purification for sequencing. .. The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems.

    Article Title: TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation
    Article Snippet: Purified PCR fragments were roughly quantitated by agarose gel electrophoresis using SmartLadder molecular weight marker (EUROGENTEC) as a quantitative reference. .. DNA sequencing was performed using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) on an Applied Biosystems 3100 automatic DNA sequencer.

    Lysis:

    Article Title: Conventional Methods versus 16S Ribosomal DNA Sequencing for Identification of Nontuberculous Mycobacteria: Cost Analysis
    Article Snippet: The steps required for the performance of 16S rDNA sequencing included crude DNA lysate preparation by mechanical lysis using the Mini-Beadbeater (Biospec Products, Bartlesville, Okla.), DNA quantitation, PCR, electrophoresis, and sequencing. .. The actual sequencing reaction performed for each sample required Sequencing Polymer (POP-6), ABI Prism BigDye Terminator cycle sequencing ready reaction kit, and consumables as recommended from Applied Biosystems.

    Homologous Recombination:

    Article Title: Mapping the Rubella Virus Subgenomic Promoter
    Article Snippet: Reverse transcription (RT)-PCR amplification and sequencing were used to confirm the presence of intended mutations in viable virus recovered following transfection with transcripts from mutated constructs and to examine potential genomic rearrangements produced by homologous recombination between the two SGPs in dsRobo402/GFP constructs. .. Automated sequencing was performed by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Applied Biosystems, Foster City, Calif.) with oligonucleotide 177 as the primer and an ABI373 sequencer (Perkin-Elmer Corporation, Foster City, Calif.).

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    Thermo Fisher abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing ready reaction kit/product/Thermo Fisher
    Average 99 stars, based on 393 article reviews
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