abi prism bigdye terminator cycle sequencing ready reaction kit  (PerkinElmer)

 
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    Structured Review

    PerkinElmer abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing ready reaction kit/product/PerkinElmer
    Average 92 stars, based on 117 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator cycle sequencing ready reaction kit - by Bioz Stars, 2020-07
    92/100 stars

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    Amplification:

    Article Title: Molecular Evolution of a Type 1 Wild-Vaccine Poliovirus Recombinant during Widespread Circulation in China
    Article Snippet: .. Amplified PCR products were purified with a QIAQuick PCR purification kit (QIAGEN, Chatsworth, Calif.) and used directly for sequencing with the dideoxynucleotide method, using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Perkin-Elmer Applied Biosystems, Foster City, Calif.) and an automated DNA sequencer (model 377A; Perkin-Elmer Applied Biosystems). .. The four PCR primers and four additional sequencing primers (H4S [2717 to 2736, 5′-TTCTTTGCGCGGGGTGCATG-3′], H3A [2753 to 2772, 5′-GTGGTAGCCGAATTGTCCAC-3′], H6S [3410 to 3429, 5′-GTGTACACTGCAGGTTACAA-3′], and Q8A [3485 to 3504, 5′-AAGAGGTCTCTATTCCACAT-3′]) ( ) were used for sequencing the PCR amplicons.

    Purification:

    Article Title: Molecular Evolution of a Type 1 Wild-Vaccine Poliovirus Recombinant during Widespread Circulation in China
    Article Snippet: .. Amplified PCR products were purified with a QIAQuick PCR purification kit (QIAGEN, Chatsworth, Calif.) and used directly for sequencing with the dideoxynucleotide method, using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Perkin-Elmer Applied Biosystems, Foster City, Calif.) and an automated DNA sequencer (model 377A; Perkin-Elmer Applied Biosystems). .. The four PCR primers and four additional sequencing primers (H4S [2717 to 2736, 5′-TTCTTTGCGCGGGGTGCATG-3′], H3A [2753 to 2772, 5′-GTGGTAGCCGAATTGTCCAC-3′], H6S [3410 to 3429, 5′-GTGTACACTGCAGGTTACAA-3′], and Q8A [3485 to 3504, 5′-AAGAGGTCTCTATTCCACAT-3′]) ( ) were used for sequencing the PCR amplicons.

    Article Title: Vicariance and Its Impact on the Molecular Ecology of a Chinese Ranid Frog Species-Complex (Odorrana schmackeri, Ranidae)
    Article Snippet: .. The DNA products were purified, and then sequenced in both directions on an ABI3730 with an ABI PRISM BigDye terminator Cycle Sequencing Ready Reaction Kit (Perkin-Elmer Biosystems). .. Sequences alignment and phylogenetic analyses Sequences were edited and aligned manually using BIOEDIT version 7.0.9.0 [ ], and the protein coding region ND2 was translated into amino acids for confirmation of alignment.

    Article Title: Ixodes ricinus ticks are reservoir hosts for Rickettsia helvetica and potentially carry flea-borne Rickettsia species
    Article Snippet: .. PCR amplicons were purified with the Qiaquick gel extraction kit (Qiagen Inc.) and sequenced using an ABI PRISM BigDye Terminator Cycle sequencing Ready Reaction kit (Perkin Elmer, Applied Biosystems). .. All sequences were confirmed by sequencing both strands, and unique sequences were submitted to Genbank ( GQ849209 - GQ849220 ).

    Polymerase Chain Reaction:

    Article Title: Molecular Evolution of a Type 1 Wild-Vaccine Poliovirus Recombinant during Widespread Circulation in China
    Article Snippet: .. Amplified PCR products were purified with a QIAQuick PCR purification kit (QIAGEN, Chatsworth, Calif.) and used directly for sequencing with the dideoxynucleotide method, using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Perkin-Elmer Applied Biosystems, Foster City, Calif.) and an automated DNA sequencer (model 377A; Perkin-Elmer Applied Biosystems). .. The four PCR primers and four additional sequencing primers (H4S [2717 to 2736, 5′-TTCTTTGCGCGGGGTGCATG-3′], H3A [2753 to 2772, 5′-GTGGTAGCCGAATTGTCCAC-3′], H6S [3410 to 3429, 5′-GTGTACACTGCAGGTTACAA-3′], and Q8A [3485 to 3504, 5′-AAGAGGTCTCTATTCCACAT-3′]) ( ) were used for sequencing the PCR amplicons.

    Article Title: Ixodes ricinus ticks are reservoir hosts for Rickettsia helvetica and potentially carry flea-borne Rickettsia species
    Article Snippet: .. PCR amplicons were purified with the Qiaquick gel extraction kit (Qiagen Inc.) and sequenced using an ABI PRISM BigDye Terminator Cycle sequencing Ready Reaction kit (Perkin Elmer, Applied Biosystems). .. All sequences were confirmed by sequencing both strands, and unique sequences were submitted to Genbank ( GQ849209 - GQ849220 ).

    Article Title: The Expression of the High Mobility Group I(Y) mRNA in Thyroid Cancers: Useful Tool of Differential Diagnosis of Thyroid Nodules
    Article Snippet: .. Nucleotide Sequencing PCR products were sequenced using an ABI Prism Automated DNA Sequencer(model 310, PE Applied Biosystems, USA)and the ABI Prism BigDye Terminator cycle Sequencing Ready Reaction Kit(Perkin-Elmer Co., USA). .. Statistical analysis The difference of HMG I(Y) expression between benign and malignant tumors was analyzed using Student t-test. p value less than 0.05 was considered significant.

    Gel Extraction:

    Article Title: A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning
    Article Snippet: .. QIAquick Gel Extraction Kit (Qiagen, cat. no. 28704) QIAprep Spin Miniprep Kit (Qiagen, cat. no. 27104) PureYield Plasmid Maxiprep System (Promega, cat. no. A2392) ABI PRISM BigDye terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystems, cat. no. 4303573) ECL western blotting detection reagents (Amersham Pharmacia Biotech, cat. no. RPN 2209) Micro BCA Protein Assay Kit (Pierce, cat. no. Pi-23235) .. Tris-HCl buffers (1 M (pH 8) and 0.5 M (pH 6.8); see REAGENT SETUP) EDTA (0.5 M (pH 8); see REAGENT SETUP) TE buffer (see REAGENT SETUP) LB medium (Luria Bertani broth; see REAGENT SETUP) GTE buffer (50 mM d -glucose, 25 mM Tris-HCl (pH 8), 10 mM EDTA; see ref. for preparation and storage) Alkaline lysis buffer (0.2 M NaOH, 1% SDS; see ref. for preparation and storage) RNAse A 10 mg ml−1 stock (see ref. for preparation and storage) Potassium acetate buffer (pH 4.8; see ref. for preparation and storage) DNAse I 2 mg ml−1 stock (see ref. for preparation and storage) Lysis buffers (1× and 8×; see REAGENT SETUP) Charge buffer (see REAGENT SETUP) Binding buffers (1× and 8×; see REAGENT SETUP) Wash buffers (see REAGENT SETUP) Elution buffers (see REAGENT SETUP) Strip buffer (see REAGENT SETUP) Running buffer for agarose gels electrophoresis (50× and 1× TAE buffers; see REAGENT SETUP) Sample buffer (2× SDS reducing buffer; see REAGENT SETUP) 10× electrode buffer for SDS-PAGE (10× EB; see REAGENT SETUP) 10× phosphate-buffered saline (PBS) buffer (see REAGENT SETUP) 1× PBS-Tween (PBST) buffer (see REAGENT SETUP) Blocking buffer (see REAGENT SETUP)

    Article Title: Ixodes ricinus ticks are reservoir hosts for Rickettsia helvetica and potentially carry flea-borne Rickettsia species
    Article Snippet: .. PCR amplicons were purified with the Qiaquick gel extraction kit (Qiagen Inc.) and sequenced using an ABI PRISM BigDye Terminator Cycle sequencing Ready Reaction kit (Perkin Elmer, Applied Biosystems). .. All sequences were confirmed by sequencing both strands, and unique sequences were submitted to Genbank ( GQ849209 - GQ849220 ).

    BIA-KA:

    Article Title: A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning
    Article Snippet: .. QIAquick Gel Extraction Kit (Qiagen, cat. no. 28704) QIAprep Spin Miniprep Kit (Qiagen, cat. no. 27104) PureYield Plasmid Maxiprep System (Promega, cat. no. A2392) ABI PRISM BigDye terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystems, cat. no. 4303573) ECL western blotting detection reagents (Amersham Pharmacia Biotech, cat. no. RPN 2209) Micro BCA Protein Assay Kit (Pierce, cat. no. Pi-23235) .. Tris-HCl buffers (1 M (pH 8) and 0.5 M (pH 6.8); see REAGENT SETUP) EDTA (0.5 M (pH 8); see REAGENT SETUP) TE buffer (see REAGENT SETUP) LB medium (Luria Bertani broth; see REAGENT SETUP) GTE buffer (50 mM d -glucose, 25 mM Tris-HCl (pH 8), 10 mM EDTA; see ref. for preparation and storage) Alkaline lysis buffer (0.2 M NaOH, 1% SDS; see ref. for preparation and storage) RNAse A 10 mg ml−1 stock (see ref. for preparation and storage) Potassium acetate buffer (pH 4.8; see ref. for preparation and storage) DNAse I 2 mg ml−1 stock (see ref. for preparation and storage) Lysis buffers (1× and 8×; see REAGENT SETUP) Charge buffer (see REAGENT SETUP) Binding buffers (1× and 8×; see REAGENT SETUP) Wash buffers (see REAGENT SETUP) Elution buffers (see REAGENT SETUP) Strip buffer (see REAGENT SETUP) Running buffer for agarose gels electrophoresis (50× and 1× TAE buffers; see REAGENT SETUP) Sample buffer (2× SDS reducing buffer; see REAGENT SETUP) 10× electrode buffer for SDS-PAGE (10× EB; see REAGENT SETUP) 10× phosphate-buffered saline (PBS) buffer (see REAGENT SETUP) 1× PBS-Tween (PBST) buffer (see REAGENT SETUP) Blocking buffer (see REAGENT SETUP)

    Sequencing:

    Article Title: Molecular Evolution of a Type 1 Wild-Vaccine Poliovirus Recombinant during Widespread Circulation in China
    Article Snippet: .. Amplified PCR products were purified with a QIAQuick PCR purification kit (QIAGEN, Chatsworth, Calif.) and used directly for sequencing with the dideoxynucleotide method, using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Perkin-Elmer Applied Biosystems, Foster City, Calif.) and an automated DNA sequencer (model 377A; Perkin-Elmer Applied Biosystems). .. The four PCR primers and four additional sequencing primers (H4S [2717 to 2736, 5′-TTCTTTGCGCGGGGTGCATG-3′], H3A [2753 to 2772, 5′-GTGGTAGCCGAATTGTCCAC-3′], H6S [3410 to 3429, 5′-GTGTACACTGCAGGTTACAA-3′], and Q8A [3485 to 3504, 5′-AAGAGGTCTCTATTCCACAT-3′]) ( ) were used for sequencing the PCR amplicons.

    Article Title: A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning
    Article Snippet: .. QIAquick Gel Extraction Kit (Qiagen, cat. no. 28704) QIAprep Spin Miniprep Kit (Qiagen, cat. no. 27104) PureYield Plasmid Maxiprep System (Promega, cat. no. A2392) ABI PRISM BigDye terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystems, cat. no. 4303573) ECL western blotting detection reagents (Amersham Pharmacia Biotech, cat. no. RPN 2209) Micro BCA Protein Assay Kit (Pierce, cat. no. Pi-23235) .. Tris-HCl buffers (1 M (pH 8) and 0.5 M (pH 6.8); see REAGENT SETUP) EDTA (0.5 M (pH 8); see REAGENT SETUP) TE buffer (see REAGENT SETUP) LB medium (Luria Bertani broth; see REAGENT SETUP) GTE buffer (50 mM d -glucose, 25 mM Tris-HCl (pH 8), 10 mM EDTA; see ref. for preparation and storage) Alkaline lysis buffer (0.2 M NaOH, 1% SDS; see ref. for preparation and storage) RNAse A 10 mg ml−1 stock (see ref. for preparation and storage) Potassium acetate buffer (pH 4.8; see ref. for preparation and storage) DNAse I 2 mg ml−1 stock (see ref. for preparation and storage) Lysis buffers (1× and 8×; see REAGENT SETUP) Charge buffer (see REAGENT SETUP) Binding buffers (1× and 8×; see REAGENT SETUP) Wash buffers (see REAGENT SETUP) Elution buffers (see REAGENT SETUP) Strip buffer (see REAGENT SETUP) Running buffer for agarose gels electrophoresis (50× and 1× TAE buffers; see REAGENT SETUP) Sample buffer (2× SDS reducing buffer; see REAGENT SETUP) 10× electrode buffer for SDS-PAGE (10× EB; see REAGENT SETUP) 10× phosphate-buffered saline (PBS) buffer (see REAGENT SETUP) 1× PBS-Tween (PBST) buffer (see REAGENT SETUP) Blocking buffer (see REAGENT SETUP)

    Article Title: Vicariance and Its Impact on the Molecular Ecology of a Chinese Ranid Frog Species-Complex (Odorrana schmackeri, Ranidae)
    Article Snippet: .. The DNA products were purified, and then sequenced in both directions on an ABI3730 with an ABI PRISM BigDye terminator Cycle Sequencing Ready Reaction Kit (Perkin-Elmer Biosystems). .. Sequences alignment and phylogenetic analyses Sequences were edited and aligned manually using BIOEDIT version 7.0.9.0 [ ], and the protein coding region ND2 was translated into amino acids for confirmation of alignment.

    Article Title: Genome-wide recombination drives diversification of epidemic strains of Acinetobacter baumannii
    Article Snippet: .. Sequencing reactions were performed with the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (PerkinElmer Applied Biosystems). .. Data analysis and sequence alignments were carried out with the Lasergene suite MegAlign software (DNASTAR).

    Article Title: Ixodes ricinus ticks are reservoir hosts for Rickettsia helvetica and potentially carry flea-borne Rickettsia species
    Article Snippet: .. PCR amplicons were purified with the Qiaquick gel extraction kit (Qiagen Inc.) and sequenced using an ABI PRISM BigDye Terminator Cycle sequencing Ready Reaction kit (Perkin Elmer, Applied Biosystems). .. All sequences were confirmed by sequencing both strands, and unique sequences were submitted to Genbank ( GQ849209 - GQ849220 ).

    Article Title: Multiplex amplification refractory mutation system (MARMS) for the detection of β-globin gene mutations among the transfusion-dependent β-thalassemia Malay patients in Kelantan, Northeast of Peninsular Malaysia
    Article Snippet: .. Based on the results, those which were not detected by MARMS were sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer, Applied Biosystems Division, USA). ..

    Article Title: Prevalence of Polymorphisms in Antifolate Drug Resistance Molecular Marker Genes pvdhfr and pvdhps in Clinical Isolates of Plasmodium vivax from Kolkata, India
    Article Snippet: .. Sequencing reactions were carried out with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit on a 3730 XL genetic analyzer (PerkinElmer, Branchburg, NJ, USA).The sequences were analyzed using the free software Bioedit Sequence Alignment Editor, version 7.0.5.2 ( ). .. The sequences were then aligned using the online sequence alignment tool ClustalW (available at ).

    Article Title: The Expression of the High Mobility Group I(Y) mRNA in Thyroid Cancers: Useful Tool of Differential Diagnosis of Thyroid Nodules
    Article Snippet: .. Nucleotide Sequencing PCR products were sequenced using an ABI Prism Automated DNA Sequencer(model 310, PE Applied Biosystems, USA)and the ABI Prism BigDye Terminator cycle Sequencing Ready Reaction Kit(Perkin-Elmer Co., USA). .. Statistical analysis The difference of HMG I(Y) expression between benign and malignant tumors was analyzed using Student t-test. p value less than 0.05 was considered significant.

    Western Blot:

    Article Title: A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning
    Article Snippet: .. QIAquick Gel Extraction Kit (Qiagen, cat. no. 28704) QIAprep Spin Miniprep Kit (Qiagen, cat. no. 27104) PureYield Plasmid Maxiprep System (Promega, cat. no. A2392) ABI PRISM BigDye terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystems, cat. no. 4303573) ECL western blotting detection reagents (Amersham Pharmacia Biotech, cat. no. RPN 2209) Micro BCA Protein Assay Kit (Pierce, cat. no. Pi-23235) .. Tris-HCl buffers (1 M (pH 8) and 0.5 M (pH 6.8); see REAGENT SETUP) EDTA (0.5 M (pH 8); see REAGENT SETUP) TE buffer (see REAGENT SETUP) LB medium (Luria Bertani broth; see REAGENT SETUP) GTE buffer (50 mM d -glucose, 25 mM Tris-HCl (pH 8), 10 mM EDTA; see ref. for preparation and storage) Alkaline lysis buffer (0.2 M NaOH, 1% SDS; see ref. for preparation and storage) RNAse A 10 mg ml−1 stock (see ref. for preparation and storage) Potassium acetate buffer (pH 4.8; see ref. for preparation and storage) DNAse I 2 mg ml−1 stock (see ref. for preparation and storage) Lysis buffers (1× and 8×; see REAGENT SETUP) Charge buffer (see REAGENT SETUP) Binding buffers (1× and 8×; see REAGENT SETUP) Wash buffers (see REAGENT SETUP) Elution buffers (see REAGENT SETUP) Strip buffer (see REAGENT SETUP) Running buffer for agarose gels electrophoresis (50× and 1× TAE buffers; see REAGENT SETUP) Sample buffer (2× SDS reducing buffer; see REAGENT SETUP) 10× electrode buffer for SDS-PAGE (10× EB; see REAGENT SETUP) 10× phosphate-buffered saline (PBS) buffer (see REAGENT SETUP) 1× PBS-Tween (PBST) buffer (see REAGENT SETUP) Blocking buffer (see REAGENT SETUP)

    Plasmid Preparation:

    Article Title: A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning
    Article Snippet: .. QIAquick Gel Extraction Kit (Qiagen, cat. no. 28704) QIAprep Spin Miniprep Kit (Qiagen, cat. no. 27104) PureYield Plasmid Maxiprep System (Promega, cat. no. A2392) ABI PRISM BigDye terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystems, cat. no. 4303573) ECL western blotting detection reagents (Amersham Pharmacia Biotech, cat. no. RPN 2209) Micro BCA Protein Assay Kit (Pierce, cat. no. Pi-23235) .. Tris-HCl buffers (1 M (pH 8) and 0.5 M (pH 6.8); see REAGENT SETUP) EDTA (0.5 M (pH 8); see REAGENT SETUP) TE buffer (see REAGENT SETUP) LB medium (Luria Bertani broth; see REAGENT SETUP) GTE buffer (50 mM d -glucose, 25 mM Tris-HCl (pH 8), 10 mM EDTA; see ref. for preparation and storage) Alkaline lysis buffer (0.2 M NaOH, 1% SDS; see ref. for preparation and storage) RNAse A 10 mg ml−1 stock (see ref. for preparation and storage) Potassium acetate buffer (pH 4.8; see ref. for preparation and storage) DNAse I 2 mg ml−1 stock (see ref. for preparation and storage) Lysis buffers (1× and 8×; see REAGENT SETUP) Charge buffer (see REAGENT SETUP) Binding buffers (1× and 8×; see REAGENT SETUP) Wash buffers (see REAGENT SETUP) Elution buffers (see REAGENT SETUP) Strip buffer (see REAGENT SETUP) Running buffer for agarose gels electrophoresis (50× and 1× TAE buffers; see REAGENT SETUP) Sample buffer (2× SDS reducing buffer; see REAGENT SETUP) 10× electrode buffer for SDS-PAGE (10× EB; see REAGENT SETUP) 10× phosphate-buffered saline (PBS) buffer (see REAGENT SETUP) 1× PBS-Tween (PBST) buffer (see REAGENT SETUP) Blocking buffer (see REAGENT SETUP)

    Software:

    Article Title: Prevalence of Polymorphisms in Antifolate Drug Resistance Molecular Marker Genes pvdhfr and pvdhps in Clinical Isolates of Plasmodium vivax from Kolkata, India
    Article Snippet: .. Sequencing reactions were carried out with an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit on a 3730 XL genetic analyzer (PerkinElmer, Branchburg, NJ, USA).The sequences were analyzed using the free software Bioedit Sequence Alignment Editor, version 7.0.5.2 ( ). .. The sequences were then aligned using the online sequence alignment tool ClustalW (available at ).

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    PerkinElmer abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing ready reaction kit/product/PerkinElmer
    Average 92 stars, based on 117 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator cycle sequencing ready reaction kit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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