abi prism bigdye terminator cycle sequencing kit  (Thermo Fisher)


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    Thermo Fisher abi prism bigdye terminator cycle sequencing kit
    Abi Prism Bigdye Terminator Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 212 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator cycle sequencing kit - by Bioz Stars, 2020-04
    99/100 stars

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    Diagnostic Assay:

    Article Title: Cystic fibrosis testing in a referral laboratory: results and lessons from a six-year period
    Article Snippet: Indications for testing included carrier testing in healthy individuals, confirmation or diagnostic testing in patient with classically affected CF, and diagnostic testing for patients with potentially CF-related symptoms, but without a diagnosis of CF. .. Samples were sequenced bi-directionally by a Sanger method using dye-terminator chemistry (BigDye® Direct Cycle Sequencing kit; Life Technologies, Carlsbad, CA) with M13-tailed primers.

    Clone Assay:

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: The pET-28(b)+/16E1^E4 expression vector was made by cloning the 16E1^E4 BamHI-XhoI fragment into the pET-28(b)+ construct (Novagen, United Kingdom). .. The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom).

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: Those samples positive for Plasmodium were each purified using Gel/PCR DNAFragment Extraction kit (Geneaid, Taiwan), cloned into the Zero Blunt® vector (ThermoFisher Scientific), transformed into One Shot® TOPO10 Chemically Competent E. coli using heat-shock, and then plated onto LB agar containing (50 μg/mL) kanamycin. .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Amplification:

    Article Title: Severely Incapacitating Mutations in Patients with Extreme Short Stature Identify RNA-Processing Endoribonuclease RMRP as an Essential Cell Growth Regulator
    Article Snippet: Concerning the RMRP gene, we amplified the coding region, the promoter region, and the TATA box in all members of family 1 and 2 and in patient 3. .. The PCR products were purified with Filter plates LSKMPCR50 (Millipore) on a Tecan liquid handling system, and sequencing reactions were performed on both strands with the use of the BigDye Terminator Cycle Sequencing Kit (v. 3.1 [Applied Biosystems]), in accordance with the manufacturer’s instructions.

    Article Title: A Comparison of Entecavir and Lamivudine for the Prophylaxis of Hepatitis B Virus Reactivation in Solid Tumor Patients Undergoing Systemic Cytotoxic Chemotherapy
    Article Snippet: The PCR amplifications were performed in a PCR mechanism under the following conditions: after an initial denaturation for 5 min at 94° C, samples were subjected to 35 cycles of amplification (94° C 30 s, 55° C 30 s, 72° C 1 min), followed by a final extension of 5 min at 72° C. The purification of PCR products was performed by QIAquick PCR purification kit according to manufacture’s instructions (QIAGEN, Valencia, CA, USA). .. Sequence analysis of the PCR products was performed by BigDye Direct Cycle Sequencing Kit (Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) in a ABI 3730XL according to manufacture’s instructions.

    Article Title: Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
    Article Snippet: .. DNA Amplification and Sequencing DNA sequencing was performed using a BigDye Direct Cycle Sequencing Kit (Life Technologies). .. For PCR amplification, 1 μ L of diluted DNA (10 ng/μ L) was mixed with 5.0 μ L of BigDye Direct PCR Master Mix, 0.75 μ L of each forward ITS1-M13 (5′-TGTAAAACGACGGCCAGTTCCGTAGGTGAACCTGCGG-3′) and reverse ITS4-M13 (5′-CAGGAAACAGCTATGACCTCCTCCGCTTATTGATATGC-3′) primers at a 10 μ M concentration, which include the M13 universal primer sequences, and 2.4 μ L of ultrapure water.

    Article Title: Intersubtype Reassortments of H5N1 Highly Pathogenic Avian Influenza Viruses Isolated from Quail
    Article Snippet: Nucleotide sequencing and sequence analysis The amplified PCR products were purified using a QIAquick Gel Extraction Kit (Qiagen). .. RT-PCR primers were used for direct sequencing of the HA, NA, PB2, PB1, PA, NP, M, and NS genes using a BigDye Terminator Cycle Sequencing Kit and an ABI 3730 DNA sequencer (Applied Biosystems).

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: The predicted sizes of the amplified PCR products for Anaplasma , Ehrlichia , and Rickettsia were 429 bp, 340 bp, and 252 bp, respectively. .. The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems).

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: PCR amplification of the ClpM gene was performed in a 20 μL reaction mixture containing 2 μL of purified genomic DNA, 200 μM each deoxyribonucleotide triphosphate (dNTP) (Promega,Madison WI, USA), Phusion HF buffer (1.5 mM MgCl2 ), 0.02 U/ μL of Phusion DNA polymerase (ThermoScientific, USA) and 0.5 μM of each primer (ACLP-F1 and ACLP-R1) under the following conditions: 98 °C for 30s for first denaturation, 35 cycles at 98 °C for 7 s, 60 °C for 20s and 72 °C for 30s, followed by a final extension for 10mins at 72 °C. .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Polymerase Chain Reaction:

    Article Title: Severely Incapacitating Mutations in Patients with Extreme Short Stature Identify RNA-Processing Endoribonuclease RMRP as an Essential Cell Growth Regulator
    Article Snippet: .. The PCR products were purified with Filter plates LSKMPCR50 (Millipore) on a Tecan liquid handling system, and sequencing reactions were performed on both strands with the use of the BigDye Terminator Cycle Sequencing Kit (v. 3.1 [Applied Biosystems]), in accordance with the manufacturer’s instructions. .. After purification with filter plates LSKS09624 (Millipore), the products were analyzed on an ABI Genetic Analyzer 3730 (Applied Biosystems) with SeqMan II software (DNASTAR).

    Article Title: A Comparison of Entecavir and Lamivudine for the Prophylaxis of Hepatitis B Virus Reactivation in Solid Tumor Patients Undergoing Systemic Cytotoxic Chemotherapy
    Article Snippet: .. Sequence analysis of the PCR products was performed by BigDye Direct Cycle Sequencing Kit (Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) in a ABI 3730XL according to manufacture’s instructions. ..

    Article Title: Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
    Article Snippet: DNA Amplification and Sequencing DNA sequencing was performed using a BigDye Direct Cycle Sequencing Kit (Life Technologies). .. For PCR amplification, 1 μ L of diluted DNA (10 ng/μ L) was mixed with 5.0 μ L of BigDye Direct PCR Master Mix, 0.75 μ L of each forward ITS1-M13 (5′-TGTAAAACGACGGCCAGTTCCGTAGGTGAACCTGCGG-3′) and reverse ITS4-M13 (5′-CAGGAAACAGCTATGACCTCCTCCGCTTATTGATATGC-3′) primers at a 10 μ M concentration, which include the M13 universal primer sequences, and 2.4 μ L of ultrapure water.

    Article Title: Comparison between qualitative and real-time polymerase chain reaction to evaluate minimal residual disease in children with acute lymphoblastic leukemia
    Article Snippet: Sequencing and design of patient-specific primers Clonal PCR products from Day 0 that had been dissolved in water were re-amplified in a volume of 50 μL using the same primer sets (but with T7 or M13 extensions) and reaction conditions as described above. .. Sequencing reactions were carried out using the BigDye Terminator Cycle Sequencing Reaction Kit (PE Applied Biosystems) and T7 and M13 primers.

    Article Title: Identification of a novel ARL13B variant in a Joubert syndrome-affected patient with retinal impairment and obesity
    Article Snippet: .. PCR was performed with a touchdown protocol consisting of denaturation for 30 s at 96 °C, annealing for 30 s at a temperature ranging from 64 to 50 °C (decreasing by 1 °C during 14 cycles, then 20 cycles at 50 °C) and extension at 72 °C for 30 s. PCR products were treated with ExoSAP-IT (USB Corp., Cleveland, OH, USA), and both strands were sequenced with the appropriate primer and the ‘BigDye' terminator cycle sequencing kit (Applied Biosystems Inc., Foster City, CA, USA) and analyzed on ABI3130 automated sequencers. .. The DNA variant numbering system we used is based on cDNA sequence with +1 corresponding to the A of the ATG translation initiation codon in the reference sequence.

    Article Title: Natural Polymorphisms in the Human Immunodeficiency Virus Type 2 Protease Can Accelerate Time to Development of Resistance to Protease Inhibitors ▿
    Article Snippet: .. Cycle sequencing of both strands was performed on the GeneAmp PCR 9700 instrument with the BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA). .. Excess dye-labeled terminators were removed from the extension products and purified products were sequenced on the ABI Prism 3100 genetic analyzer (Applied Biosystems).

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: .. The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom). ..

    Article Title: Intersubtype Reassortments of H5N1 Highly Pathogenic Avian Influenza Viruses Isolated from Quail
    Article Snippet: Nucleotide sequencing and sequence analysis The amplified PCR products were purified using a QIAquick Gel Extraction Kit (Qiagen). .. RT-PCR primers were used for direct sequencing of the HA, NA, PB2, PB1, PA, NP, M, and NS genes using a BigDye Terminator Cycle Sequencing Kit and an ABI 3730 DNA sequencer (Applied Biosystems).

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: .. The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems). .. The sequence data were aligned initially using the Clustal X program (version 1.8) [ ].

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: To determine whether the transformed E. coli harbouring recombinant plasmid with ClpM insert, the colonies were examined by PCR using the ClpM-specific primers ACLP-F1 and ACLP-R1. .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Construct:

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: The pET-28(b)+/16E1^E4 expression vector was made by cloning the 16E1^E4 BamHI-XhoI fragment into the pET-28(b)+ construct (Novagen, United Kingdom). .. The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom).

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems). .. A phylogenetic tree based on the nucleotide alignment was constructed using the neighbor-joining method [ ].

    Electrophoresis:

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: PCR products were separated by electrophoresis in a 1% agarose gel, stained with SYBR® DNA gel stain (Invitrogen, USA) and visualised under a UV transilluminator (GBOX from Syngene). .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Article Title: Cystic fibrosis testing in a referral laboratory: results and lessons from a six-year period
    Article Snippet: Samples were sequenced bi-directionally by a Sanger method using dye-terminator chemistry (BigDye® Direct Cycle Sequencing kit; Life Technologies, Carlsbad, CA) with M13-tailed primers. .. Samples were sequenced bi-directionally by a Sanger method using dye-terminator chemistry (BigDye® Direct Cycle Sequencing kit; Life Technologies, Carlsbad, CA) with M13-tailed primers.

    Incubation:

    Article Title: Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
    Article Snippet: DNA Amplification and Sequencing DNA sequencing was performed using a BigDye Direct Cycle Sequencing Kit (Life Technologies). .. Following PCR, 3 μ L of BigDye Direct Sequencing Master Mix and 1 μ L of forward BigDye Direct M13 forward primer (provided) were added directly to the PCR sample, put back into the PCR thermal cycler, and incubated as follows: 15 min at 37°C, 2 min at 80°C, and 1 min at 96°C, followed by 25 cycles of 10 sec at 96°C, 5 sec at 50°C, and 75 sec at 60°C.

    Expressing:

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: The pET-28(b)+/16E1^E4 expression vector was made by cloning the 16E1^E4 BamHI-XhoI fragment into the pET-28(b)+ construct (Novagen, United Kingdom). .. The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom).

    Transformation Assay:

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: To determine whether the transformed E. coli harbouring recombinant plasmid with ClpM insert, the colonies were examined by PCR using the ClpM-specific primers ACLP-F1 and ACLP-R1. .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Derivative Assay:

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome. .. In addition to the Plasmodium sp. from macaque blood samples, ClpM gene sequences were also derived from 2 human infections in the Kapit division of Sarawak, Malaysian Borneo.

    Infection:

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: For the detection of Anaplasma infection, PCR was performed using the AccuPower ® Rickettsiales 3-Plex PCR Kit (Bioneer, Daejeon, Korea). .. The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems).

    DNA Sequencing:

    Article Title: Phylogenetic comparisons suggest that distance from the locus control region guides developmental expression of primate ?-type globin genes
    Article Snippet: .. DNA sequencing reactions were done by using the Big-Dye primer cycle sequencing kit (Applied Biosystems) according to the manufacturer’s specifications. .. Sequencing was performed on the ABI 3700 (Applied Biosystems).

    Article Title: Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
    Article Snippet: .. DNA Amplification and Sequencing DNA sequencing was performed using a BigDye Direct Cycle Sequencing Kit (Life Technologies). .. For PCR amplification, 1 μ L of diluted DNA (10 ng/μ L) was mixed with 5.0 μ L of BigDye Direct PCR Master Mix, 0.75 μ L of each forward ITS1-M13 (5′-TGTAAAACGACGGCCAGTTCCGTAGGTGAACCTGCGG-3′) and reverse ITS4-M13 (5′-CAGGAAACAGCTATGACCTCCTCCGCTTATTGATATGC-3′) primers at a 10 μ M concentration, which include the M13 universal primer sequences, and 2.4 μ L of ultrapure water.

    Article Title: Natural Polymorphisms in the Human Immunodeficiency Virus Type 2 Protease Can Accelerate Time to Development of Resistance to Protease Inhibitors ▿
    Article Snippet: Paragraph title: DNA sequencing analysis. ... Cycle sequencing of both strands was performed on the GeneAmp PCR 9700 instrument with the BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA).

    Sequencing:

    Article Title: Severely Incapacitating Mutations in Patients with Extreme Short Stature Identify RNA-Processing Endoribonuclease RMRP as an Essential Cell Growth Regulator
    Article Snippet: .. The PCR products were purified with Filter plates LSKMPCR50 (Millipore) on a Tecan liquid handling system, and sequencing reactions were performed on both strands with the use of the BigDye Terminator Cycle Sequencing Kit (v. 3.1 [Applied Biosystems]), in accordance with the manufacturer’s instructions. .. After purification with filter plates LSKS09624 (Millipore), the products were analyzed on an ABI Genetic Analyzer 3730 (Applied Biosystems) with SeqMan II software (DNASTAR).

    Article Title: A Comparison of Entecavir and Lamivudine for the Prophylaxis of Hepatitis B Virus Reactivation in Solid Tumor Patients Undergoing Systemic Cytotoxic Chemotherapy
    Article Snippet: .. Sequence analysis of the PCR products was performed by BigDye Direct Cycle Sequencing Kit (Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) in a ABI 3730XL according to manufacture’s instructions. ..

    Article Title: Phylogenetic comparisons suggest that distance from the locus control region guides developmental expression of primate ?-type globin genes
    Article Snippet: .. DNA sequencing reactions were done by using the Big-Dye primer cycle sequencing kit (Applied Biosystems) according to the manufacturer’s specifications. .. Sequencing was performed on the ABI 3700 (Applied Biosystems).

    Article Title: Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
    Article Snippet: .. DNA Amplification and Sequencing DNA sequencing was performed using a BigDye Direct Cycle Sequencing Kit (Life Technologies). .. For PCR amplification, 1 μ L of diluted DNA (10 ng/μ L) was mixed with 5.0 μ L of BigDye Direct PCR Master Mix, 0.75 μ L of each forward ITS1-M13 (5′-TGTAAAACGACGGCCAGTTCCGTAGGTGAACCTGCGG-3′) and reverse ITS4-M13 (5′-CAGGAAACAGCTATGACCTCCTCCGCTTATTGATATGC-3′) primers at a 10 μ M concentration, which include the M13 universal primer sequences, and 2.4 μ L of ultrapure water.

    Article Title: Identification of a novel ARL13B variant in a Joubert syndrome-affected patient with retinal impairment and obesity
    Article Snippet: .. PCR was performed with a touchdown protocol consisting of denaturation for 30 s at 96 °C, annealing for 30 s at a temperature ranging from 64 to 50 °C (decreasing by 1 °C during 14 cycles, then 20 cycles at 50 °C) and extension at 72 °C for 30 s. PCR products were treated with ExoSAP-IT (USB Corp., Cleveland, OH, USA), and both strands were sequenced with the appropriate primer and the ‘BigDye' terminator cycle sequencing kit (Applied Biosystems Inc., Foster City, CA, USA) and analyzed on ABI3130 automated sequencers. .. The DNA variant numbering system we used is based on cDNA sequence with +1 corresponding to the A of the ATG translation initiation codon in the reference sequence.

    Article Title: Natural Polymorphisms in the Human Immunodeficiency Virus Type 2 Protease Can Accelerate Time to Development of Resistance to Protease Inhibitors ▿
    Article Snippet: .. Cycle sequencing of both strands was performed on the GeneAmp PCR 9700 instrument with the BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA). .. Excess dye-labeled terminators were removed from the extension products and purified products were sequenced on the ABI Prism 3100 genetic analyzer (Applied Biosystems).

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: .. The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom). ..

    Article Title: Intersubtype Reassortments of H5N1 Highly Pathogenic Avian Influenza Viruses Isolated from Quail
    Article Snippet: .. RT-PCR primers were used for direct sequencing of the HA, NA, PB2, PB1, PA, NP, M, and NS genes using a BigDye Terminator Cycle Sequencing Kit and an ABI 3730 DNA sequencer (Applied Biosystems). ..

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: .. The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems). .. The sequence data were aligned initially using the Clustal X program (version 1.8) [ ].

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome. .. The products were then sequenced on a AB1377 sequencer (Applied Biosystems).

    Article Title: Cystic fibrosis testing in a referral laboratory: results and lessons from a six-year period
    Article Snippet: .. Samples were sequenced bi-directionally by a Sanger method using dye-terminator chemistry (BigDye® Direct Cycle Sequencing kit; Life Technologies, Carlsbad, CA) with M13-tailed primers. .. Sequencing products were separated and detected by capillary electrophoresis (ABI 3730; Life Technologies).

    Recombinant:

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: Recombinant plasmids containing the ClpM gene fragment were purified using PureLink® Quick Plasmid DNA Miniprep Kits (Invitrogen, USA). .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Nucleic Acid Electrophoresis:

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: The following cycling conditions were used: 95˚C for 15 min; 40 cycles of 95˚C for 10 sec, 58˚C for 30 sec, and 72˚C for 30 sec; and final extension at 72˚C for 5 min. PCR products were separated by gel electrophoresis on 1.5% agarose gels and visualized by staining with ethidium bromide. .. The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems).

    Mutagenesis:

    Article Title: Severely Incapacitating Mutations in Patients with Extreme Short Stature Identify RNA-Processing Endoribonuclease RMRP as an Essential Cell Growth Regulator
    Article Snippet: Paragraph title: Mutation Analysis ... The PCR products were purified with Filter plates LSKMPCR50 (Millipore) on a Tecan liquid handling system, and sequencing reactions were performed on both strands with the use of the BigDye Terminator Cycle Sequencing Kit (v. 3.1 [Applied Biosystems]), in accordance with the manufacturer’s instructions.

    Article Title: A Comparison of Entecavir and Lamivudine for the Prophylaxis of Hepatitis B Virus Reactivation in Solid Tumor Patients Undergoing Systemic Cytotoxic Chemotherapy
    Article Snippet: LAM mutant was determined by direct sequencing of the PCR amplification products. .. Sequence analysis of the PCR products was performed by BigDye Direct Cycle Sequencing Kit (Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) in a ABI 3730XL according to manufacture’s instructions.

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: Paragraph title: Plasmid construction, site-directed mutagenesis, and sequencing. ... The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom).

    Article Title: Cystic fibrosis testing in a referral laboratory: results and lessons from a six-year period
    Article Snippet: Samples were sequenced bi-directionally by a Sanger method using dye-terminator chemistry (BigDye® Direct Cycle Sequencing kit; Life Technologies, Carlsbad, CA) with M13-tailed primers. .. The sequence was analyzed with Mutation Surveyor® (SoftGenetics, State College, PA) by two independent reviewers and a third and final review by a board certified (ABMG) clinical molecular geneticist.

    Isolation:

    Article Title: Severely Incapacitating Mutations in Patients with Extreme Short Stature Identify RNA-Processing Endoribonuclease RMRP as an Essential Cell Growth Regulator
    Article Snippet: The PCR products were purified with Filter plates LSKMPCR50 (Millipore) on a Tecan liquid handling system, and sequencing reactions were performed on both strands with the use of the BigDye Terminator Cycle Sequencing Kit (v. 3.1 [Applied Biosystems]), in accordance with the manufacturer’s instructions. .. RNA was isolated from the fibroblasts and lymphocytes of one patient and the parents of family 2, as well as from 10 controls with normal growth development, with use of the RNeasy Mini Kit (Qiagen).

    Size-exclusion Chromatography:

    Article Title: Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
    Article Snippet: DNA Amplification and Sequencing DNA sequencing was performed using a BigDye Direct Cycle Sequencing Kit (Life Technologies). .. PCR cycling conditions were as follows: initial denaturation of 10 min at 94°C, followed by 35 cycles (30 s) of denaturation at 96°C, annealing for 15 sec at 62°C, and extension for 30 sec at 72°C.

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: The following cycling conditions were used: 95˚C for 15 min; 40 cycles of 95˚C for 10 sec, 58˚C for 30 sec, and 72˚C for 30 sec; and final extension at 72˚C for 5 min. PCR products were separated by gel electrophoresis on 1.5% agarose gels and visualized by staining with ethidium bromide. .. The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems).

    Purification:

    Article Title: Severely Incapacitating Mutations in Patients with Extreme Short Stature Identify RNA-Processing Endoribonuclease RMRP as an Essential Cell Growth Regulator
    Article Snippet: .. The PCR products were purified with Filter plates LSKMPCR50 (Millipore) on a Tecan liquid handling system, and sequencing reactions were performed on both strands with the use of the BigDye Terminator Cycle Sequencing Kit (v. 3.1 [Applied Biosystems]), in accordance with the manufacturer’s instructions. .. After purification with filter plates LSKS09624 (Millipore), the products were analyzed on an ABI Genetic Analyzer 3730 (Applied Biosystems) with SeqMan II software (DNASTAR).

    Article Title: A Comparison of Entecavir and Lamivudine for the Prophylaxis of Hepatitis B Virus Reactivation in Solid Tumor Patients Undergoing Systemic Cytotoxic Chemotherapy
    Article Snippet: The PCR amplifications were performed in a PCR mechanism under the following conditions: after an initial denaturation for 5 min at 94° C, samples were subjected to 35 cycles of amplification (94° C 30 s, 55° C 30 s, 72° C 1 min), followed by a final extension of 5 min at 72° C. The purification of PCR products was performed by QIAquick PCR purification kit according to manufacture’s instructions (QIAGEN, Valencia, CA, USA). .. Sequence analysis of the PCR products was performed by BigDye Direct Cycle Sequencing Kit (Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) in a ABI 3730XL according to manufacture’s instructions.

    Article Title: Natural Polymorphisms in the Human Immunodeficiency Virus Type 2 Protease Can Accelerate Time to Development of Resistance to Protease Inhibitors ▿
    Article Snippet: Cycle sequencing of both strands was performed on the GeneAmp PCR 9700 instrument with the BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA). .. Excess dye-labeled terminators were removed from the extension products and purified products were sequenced on the ABI Prism 3100 genetic analyzer (Applied Biosystems).

    Article Title: Intersubtype Reassortments of H5N1 Highly Pathogenic Avian Influenza Viruses Isolated from Quail
    Article Snippet: Nucleotide sequencing and sequence analysis The amplified PCR products were purified using a QIAquick Gel Extraction Kit (Qiagen). .. RT-PCR primers were used for direct sequencing of the HA, NA, PB2, PB1, PA, NP, M, and NS genes using a BigDye Terminator Cycle Sequencing Kit and an ABI 3730 DNA sequencer (Applied Biosystems).

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: The PCR products were purified with the QIAquick PCR Purification Kit (Qiagen). .. The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems).

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: Recombinant plasmids containing the ClpM gene fragment were purified using PureLink® Quick Plasmid DNA Miniprep Kits (Invitrogen, USA). .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Intersubtype Reassortments of H5N1 Highly Pathogenic Avian Influenza Viruses Isolated from Quail
    Article Snippet: .. RT-PCR primers were used for direct sequencing of the HA, NA, PB2, PB1, PA, NP, M, and NS genes using a BigDye Terminator Cycle Sequencing Kit and an ABI 3730 DNA sequencer (Applied Biosystems). ..

    Gel Extraction:

    Article Title: Intersubtype Reassortments of H5N1 Highly Pathogenic Avian Influenza Viruses Isolated from Quail
    Article Snippet: Nucleotide sequencing and sequence analysis The amplified PCR products were purified using a QIAquick Gel Extraction Kit (Qiagen). .. RT-PCR primers were used for direct sequencing of the HA, NA, PB2, PB1, PA, NP, M, and NS genes using a BigDye Terminator Cycle Sequencing Kit and an ABI 3730 DNA sequencer (Applied Biosystems).

    Plasmid Preparation:

    Article Title: Severely Incapacitating Mutations in Patients with Extreme Short Stature Identify RNA-Processing Endoribonuclease RMRP as an Essential Cell Growth Regulator
    Article Snippet: The PCR products were purified with Filter plates LSKMPCR50 (Millipore) on a Tecan liquid handling system, and sequencing reactions were performed on both strands with the use of the BigDye Terminator Cycle Sequencing Kit (v. 3.1 [Applied Biosystems]), in accordance with the manufacturer’s instructions. .. The nucleotide sequence of the human RMRP gene was aligned with the sequences of orthologs from other species with use of the Multiple Sequence Alignment algorithm (Baylor College of Medicine) and AlignX of the Vector NTI Suite 6.0 (InforMax).

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: Paragraph title: Plasmid construction, site-directed mutagenesis, and sequencing. ... The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom).

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: Recombinant plasmids containing the ClpM gene fragment were purified using PureLink® Quick Plasmid DNA Miniprep Kits (Invitrogen, USA). .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Software:

    Article Title: Severely Incapacitating Mutations in Patients with Extreme Short Stature Identify RNA-Processing Endoribonuclease RMRP as an Essential Cell Growth Regulator
    Article Snippet: The PCR products were purified with Filter plates LSKMPCR50 (Millipore) on a Tecan liquid handling system, and sequencing reactions were performed on both strands with the use of the BigDye Terminator Cycle Sequencing Kit (v. 3.1 [Applied Biosystems]), in accordance with the manufacturer’s instructions. .. After purification with filter plates LSKS09624 (Millipore), the products were analyzed on an ABI Genetic Analyzer 3730 (Applied Biosystems) with SeqMan II software (DNASTAR).

    Article Title: Comparison between qualitative and real-time polymerase chain reaction to evaluate minimal residual disease in children with acute lymphoblastic leukemia
    Article Snippet: Sequencing reactions were carried out using the BigDye Terminator Cycle Sequencing Reaction Kit (PE Applied Biosystems) and T7 and M13 primers. .. Sequences were run using the ABI Prism 3130 Genetic Analyzer (PE Applied Biosystems) and analyzed using the Chromas Lite 2.4 software (Technelysium Pty Ltd.).

    Article Title: Natural Polymorphisms in the Human Immunodeficiency Virus Type 2 Protease Can Accelerate Time to Development of Resistance to Protease Inhibitors ▿
    Article Snippet: Cycle sequencing of both strands was performed on the GeneAmp PCR 9700 instrument with the BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA). .. The nucleotide sequences of the PR genes were then assembled using GeneTool 2.0 auto-assembler software.

    Article Title: Intersubtype Reassortments of H5N1 Highly Pathogenic Avian Influenza Viruses Isolated from Quail
    Article Snippet: RT-PCR primers were used for direct sequencing of the HA, NA, PB2, PB1, PA, NP, M, and NS genes using a BigDye Terminator Cycle Sequencing Kit and an ABI 3730 DNA sequencer (Applied Biosystems). .. The resulting nucleotide and deduced amino acid sequences were aligned using the Clustal_X 2.1 program [ ] and Lasergene software (DNASTAR; Madison, WI, USA), with parameters set based on H5N1 viral sequences in the NCBI GenBank database.

    Positron Emission Tomography:

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: The pMV11-16E1^E4 ( ) and pET-28(b)+/16E1^E4 mutants were produced by PCR-based site-directed mutagenesis using the QuikChange site-directed mutagenesis kit according to the protocol of the manufacturer (Stratagene, United Kingdom). .. The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom).

    Agarose Gel Electrophoresis:

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: PCR products were separated by electrophoresis in a 1% agarose gel, stained with SYBR® DNA gel stain (Invitrogen, USA) and visualised under a UV transilluminator (GBOX from Syngene). .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Laser Capture Microdissection:

    Article Title: A Comparison of Entecavir and Lamivudine for the Prophylaxis of Hepatitis B Virus Reactivation in Solid Tumor Patients Undergoing Systemic Cytotoxic Chemotherapy
    Article Snippet: LAM mutant was determined by direct sequencing of the PCR amplification products. .. Sequence analysis of the PCR products was performed by BigDye Direct Cycle Sequencing Kit (Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) in a ABI 3730XL according to manufacture’s instructions.

    Produced:

    Article Title: Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability ▿ †
    Article Snippet: The pMV11-16E1^E4 ( ) and pET-28(b)+/16E1^E4 mutants were produced by PCR-based site-directed mutagenesis using the QuikChange site-directed mutagenesis kit according to the protocol of the manufacturer (Stratagene, United Kingdom). .. The sequencing PCR was carried out using the BigDye Terminator cycle sequencing kit, version 1.1, according to the instructions of the manufacturer (Applied Biosystems, United Kingdom).

    Concentration Assay:

    Article Title: Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
    Article Snippet: DNA Amplification and Sequencing DNA sequencing was performed using a BigDye Direct Cycle Sequencing Kit (Life Technologies). .. For PCR amplification, 1 μ L of diluted DNA (10 ng/μ L) was mixed with 5.0 μ L of BigDye Direct PCR Master Mix, 0.75 μ L of each forward ITS1-M13 (5′-TGTAAAACGACGGCCAGTTCCGTAGGTGAACCTGCGG-3′) and reverse ITS4-M13 (5′-CAGGAAACAGCTATGACCTCCTCCGCTTATTGATATGC-3′) primers at a 10 μ M concentration, which include the M13 universal primer sequences, and 2.4 μ L of ultrapure water.

    Staining:

    Article Title: Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea
    Article Snippet: The following cycling conditions were used: 95˚C for 15 min; 40 cycles of 95˚C for 10 sec, 58˚C for 30 sec, and 72˚C for 30 sec; and final extension at 72˚C for 5 min. PCR products were separated by gel electrophoresis on 1.5% agarose gels and visualized by staining with ethidium bromide. .. The nucleotide sequences were determined by direct sequencing of the PCR products using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, California, USA) and analyzed on ABI PRISM® DNA Analyzer (Applied Biosystems).

    Article Title: Malaria parasites of long-tailed macaques in Sarawak, Malaysian Borneo: a novel species and demographic and evolutionary histories
    Article Snippet: PCR products were separated by electrophoresis in a 1% agarose gel, stained with SYBR® DNA gel stain (Invitrogen, USA) and visualised under a UV transilluminator (GBOX from Syngene). .. The ClpM gene was sequenced using the BigDye® Terminator Cycle Sequencing kit (Applied Biosystems, USA) as described for the mtDNA genome.

    Variant Assay:

    Article Title: Identification of a novel ARL13B variant in a Joubert syndrome-affected patient with retinal impairment and obesity
    Article Snippet: PCR was performed with a touchdown protocol consisting of denaturation for 30 s at 96 °C, annealing for 30 s at a temperature ranging from 64 to 50 °C (decreasing by 1 °C during 14 cycles, then 20 cycles at 50 °C) and extension at 72 °C for 30 s. PCR products were treated with ExoSAP-IT (USB Corp., Cleveland, OH, USA), and both strands were sequenced with the appropriate primer and the ‘BigDye' terminator cycle sequencing kit (Applied Biosystems Inc., Foster City, CA, USA) and analyzed on ABI3130 automated sequencers. .. The DNA variant numbering system we used is based on cDNA sequence with +1 corresponding to the A of the ATG translation initiation codon in the reference sequence.

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    Thermo Fisher abi prism bigdye terminator v3 1 cycle sequencing kit
    Abi Prism Bigdye Terminator V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    abi prism bigdye terminator cycle sequencing ready reaction kit - by Bioz Stars, 2020-04
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