abi prism bigdye primer cycle sequencing kit  (Thermo Fisher)


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    Thermo Fisher abi prism bigdye primer cycle sequencing kit
    Abi Prism Bigdye Primer Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye primer cycle sequencing kit/product/Thermo Fisher
    Average 87 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye primer cycle sequencing kit - by Bioz Stars, 2020-01
    87/100 stars

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    Related Articles

    Dominant Negative Mutation:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The expression vectors encoding ESE-1, ETS-1, other ETS factors, DN-ESE-1, DN-Egr-1, and Runx2 were described previously ( , ; ; ; ; ; ). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Clone Assay:

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: Concatamers were submitted to polyacrylamide gel electrophoresis and regions ranging from 300–500 bp, 500–800 bp and 800–1000 bp were purified and ligated to pZero® -1 cloning vector. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Aphid biology: Expressed genes from alate Toxoptera citricida, the brown citrus aphid
    Article Snippet: Paragraph title: Sequencing of clones ... Sequencing reactions were performed using the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, home.appliedbiosystems.com ) along with a universal T3 primer.

    Article Title: Comparative Mapping in the Pinaceae
    Article Snippet: To use direct sequencing without cloning 33 markers were selected that amplified a single locus and had no amplification background, such as light additional bands that may interfere with sequencing. .. DNA sequences were obtained from both strands with the primers used for PCR amplification and the ABI PRISM BigDye Primer Cycle Sequencing Kit v.3.1 (Applied Biosystems, Foster City, CA).

    Transfection:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Luciferase reporter plasmids were prepared for transfection using the EndoFree plasmid maxi kit (Qiagen). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Luciferase:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Paragraph title: Luciferase reporter constructs and expression plasmids ... The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Expressing:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Paragraph title: Luciferase reporter constructs and expression plasmids ... The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Purification:

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: Concatamers were submitted to polyacrylamide gel electrophoresis and regions ranging from 300–500 bp, 500–800 bp and 800–1000 bp were purified and ligated to pZero® -1 cloning vector. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Ligation:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Deletion constructs containing –577/+125 bp, –403/+125 bp, –131/+125 bp, –90/+125 bp were prepared by restriction enzyme digestion of pGL2-COL2 at PstI, NheI, NcoI, ApaI, and PvuII sites, respectively, followed by ligation. .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: Ligation reactions were used to transform One Shot® TOP10 Eletrocomp™ E. coli cells using 0.2 cm cuvettes and a Gene Pulser II electroporator (Bio-Rad Laboratories, Hercules, CA, USA) set at 2.5 kV, 25 mF and 200 Ω. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Mutagenesis:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutations in ETS sites in the pGL2-COL2 construct were generated using the PCR-based QuikChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Construct:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The expression vectors encoding ESE-1, ETS-1, other ETS factors, DN-ESE-1, DN-Egr-1, and Runx2 were described previously ( , ; ; ; ; ; ). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems). .. Transient transfection experiments were carried out in C-28/I2 cells using LipofectAMINE PLUS™ Reagent (Invitrogen).

    Colony Assay:

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: Cells were plated on low salt LB agar containing Zeocin® , in plates compatible with the automated colony picker QPix2 (Genetix, New Milton, Hampshire, UK). .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Sequencing:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The expression vectors encoding ESE-1, ETS-1, other ETS factors, DN-ESE-1, DN-Egr-1, and Runx2 were described previously ( , ; ; ; ; ; ). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems). .. Transient transfection experiments were carried out in C-28/I2 cells using LipofectAMINE PLUS™ Reagent (Invitrogen).

    Article Title: Analysis and Functional Annotation of Expressed Sequence Tags from the Asian Longhorned Beetle, Anoplophora glabripennis
    Article Snippet: Plasmid DNA was extracted using the Qiagen Liquid Handling Robot (Model 9600) in conjunction with the QIAprep 96 Turbo Miniprep Kit according to the recommended protocol. .. Single-pass sequencing was performed using the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, www.appliedbiosystems.com ) and a universal T3 primer. .. Reaction products were precipitated, resuspended in 15 µl sterile water, and loaded onto an ABI 3730 DNA Analyzer (Applied Biosystems).

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: An aliquot of each well was then used directly in a PCR reaction, with forward and reverse M13 primers. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. Three SAGE libraries were generated using two larynx cancer samples (one with lymph node metastasis or N+ and one with no lymph node metastasis or N0) and a normal control library pooled from two normal samples (surgical margins from one N+ and one N0 larynx cancer).

    Article Title: Genetic variants of HvCbf14 are statistically associated with frost tolerance in a European germplasm collection of Hordeum vulgare
    Article Snippet: Gapdh and α-Amy were amplified following the published protocol (Kilian et al. ). .. PCR products were sequenced on both strands using ABI PRISM BigDye Primer Cycle sequencing kit v.3.1 (Applied Biosystems, Foster City, USA), following manufacturer’s instruction. .. Purified sequencing reactions were injected in ABI3730 DNA Analyzer (Applied Biosystems Foster City, USA).

    Article Title: Aphid biology: Expressed genes from alate Toxoptera citricida, the brown citrus aphid
    Article Snippet: Plasmid DNA was extracted using the Qiagen 9600 liquid handling robot and the QIAprep 96 Turbo miniprep kit according to the recommended protocol (QIAGEN, www.quigen.com ). .. Sequencing reactions were performed using the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, home.appliedbiosystems.com ) along with a universal T3 primer. .. Reactions were prepared in 96-well format using the Biomek2000™ liquid handling robot (Beckman Coulter, www.beckman.com ).

    Article Title: Comparative Mapping in the Pinaceae
    Article Snippet: Four alleles at each locus were sequenced using DNA extracted from the haploid megagametophyte tissue of four Douglas fir seeds. .. DNA sequences were obtained from both strands with the primers used for PCR amplification and the ABI PRISM BigDye Primer Cycle Sequencing Kit v.3.1 (Applied Biosystems, Foster City, CA). .. Fragments were detected on an ABI 3730 DNA Analyzer at the Genomics Facility Center at the University of California, Davis.

    Article Title: Use of TaqMan 5? Nuclease Real-Time PCR for Quantitative Detection of Mycoplasma genitalium DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic
    Article Snippet: Lysates were also prepared from the broth cultures of 10 available M. genitalium strains (Table ), and the DNA was amplified with the same primers. .. The amplicons were sequenced in both directions with the MgPa1 and MgPa3 primers with the ABI PRISM BigDye Primer Cycle Sequencing kit with dye terminators (Applied Biosystems). .. Sequences were read on an ABI PRISM 377 sequencer (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutations in ETS sites in the pGL2-COL2 construct were generated using the PCR-based QuikChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: An aliquot of each well was then used directly in a PCR reaction, with forward and reverse M13 primers. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Genetic variants of HvCbf14 are statistically associated with frost tolerance in a European germplasm collection of Hordeum vulgare
    Article Snippet: Gapdh and α-Amy were amplified following the published protocol (Kilian et al. ). .. PCR products were sequenced on both strands using ABI PRISM BigDye Primer Cycle sequencing kit v.3.1 (Applied Biosystems, Foster City, USA), following manufacturer’s instruction. .. Purified sequencing reactions were injected in ABI3730 DNA Analyzer (Applied Biosystems Foster City, USA).

    Article Title: Comparative Mapping in the Pinaceae
    Article Snippet: Four alleles at each locus were sequenced using DNA extracted from the haploid megagametophyte tissue of four Douglas fir seeds. .. DNA sequences were obtained from both strands with the primers used for PCR amplification and the ABI PRISM BigDye Primer Cycle Sequencing Kit v.3.1 (Applied Biosystems, Foster City, CA). .. Fragments were detected on an ABI 3730 DNA Analyzer at the Genomics Facility Center at the University of California, Davis.

    Generated:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutations in ETS sites in the pGL2-COL2 construct were generated using the PCR-based QuikChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Amplification:

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: An aliquot of each well was then used directly in a PCR reaction, with forward and reverse M13 primers. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. Three SAGE libraries were generated using two larynx cancer samples (one with lymph node metastasis or N+ and one with no lymph node metastasis or N0) and a normal control library pooled from two normal samples (surgical margins from one N+ and one N0 larynx cancer).

    Article Title: Genetic variants of HvCbf14 are statistically associated with frost tolerance in a European germplasm collection of Hordeum vulgare
    Article Snippet: Paragraph title: PCR amplification and SNP discovery ... PCR products were sequenced on both strands using ABI PRISM BigDye Primer Cycle sequencing kit v.3.1 (Applied Biosystems, Foster City, USA), following manufacturer’s instruction.

    Article Title: Comparative Mapping in the Pinaceae
    Article Snippet: Four alleles at each locus were sequenced using DNA extracted from the haploid megagametophyte tissue of four Douglas fir seeds. .. DNA sequences were obtained from both strands with the primers used for PCR amplification and the ABI PRISM BigDye Primer Cycle Sequencing Kit v.3.1 (Applied Biosystems, Foster City, CA). .. Fragments were detected on an ABI 3730 DNA Analyzer at the Genomics Facility Center at the University of California, Davis.

    Article Title: Use of TaqMan 5? Nuclease Real-Time PCR for Quantitative Detection of Mycoplasma genitalium DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic
    Article Snippet: Lysates were also prepared from the broth cultures of 10 available M. genitalium strains (Table ), and the DNA was amplified with the same primers. .. The amplicons were sequenced in both directions with the MgPa1 and MgPa3 primers with the ABI PRISM BigDye Primer Cycle Sequencing kit with dye terminators (Applied Biosystems).

    Activity Assay:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter. .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    DNA Sequencing:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The expression vectors encoding ESE-1, ETS-1, other ETS factors, DN-ESE-1, DN-Egr-1, and Runx2 were described previously ( , ; ; ; ; ; ). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems). .. Transient transfection experiments were carried out in C-28/I2 cells using LipofectAMINE PLUS™ Reagent (Invitrogen).

    Article Title: Comparative Mapping in the Pinaceae
    Article Snippet: Paragraph title: DNA sequencing: ... DNA sequences were obtained from both strands with the primers used for PCR amplification and the ABI PRISM BigDye Primer Cycle Sequencing Kit v.3.1 (Applied Biosystems, Foster City, CA).

    Article Title: Use of TaqMan 5? Nuclease Real-Time PCR for Quantitative Detection of Mycoplasma genitalium DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic
    Article Snippet: Paragraph title: DNA sequencing. ... The amplicons were sequenced in both directions with the MgPa1 and MgPa3 primers with the ABI PRISM BigDye Primer Cycle Sequencing kit with dye terminators (Applied Biosystems).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: Concatamers were submitted to polyacrylamide gel electrophoresis and regions ranging from 300–500 bp, 500–800 bp and 800–1000 bp were purified and ligated to pZero® -1 cloning vector. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    CTG Assay:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter. .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Binding Assay:

    Article Title: Use of TaqMan 5? Nuclease Real-Time PCR for Quantitative Detection of Mycoplasma genitalium DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic
    Article Snippet: In order to confirm the sequence conservation in the primer and probe binding sites, the DNA in 32 specimens from 30 randomly selected M. genitalium -positive patients were amplified with the MgPa1 and MgPa3 primer set ( ). .. The amplicons were sequenced in both directions with the MgPa1 and MgPa3 primers with the ABI PRISM BigDye Primer Cycle Sequencing kit with dye terminators (Applied Biosystems).

    Plasmid Preparation:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Luciferase reporter plasmids were prepared for transfection using the EndoFree plasmid maxi kit (Qiagen). .. The sequences of all constructs were confirmed by DNA sequencing performed at the Beth Israel Deaconess Medical Center DNA sequencing facility using the ABI Prism BigDye™Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and the Automatic DNA Sequencer Model 373A (Applied Biosystems).

    Article Title: Analysis and Functional Annotation of Expressed Sequence Tags from the Asian Longhorned Beetle, Anoplophora glabripennis
    Article Snippet: Plasmid DNA was extracted using the Qiagen Liquid Handling Robot (Model 9600) in conjunction with the QIAprep 96 Turbo Miniprep Kit according to the recommended protocol. .. Single-pass sequencing was performed using the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, www.appliedbiosystems.com ) and a universal T3 primer.

    Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
    Article Snippet: Concatamers were submitted to polyacrylamide gel electrophoresis and regions ranging from 300–500 bp, 500–800 bp and 800–1000 bp were purified and ligated to pZero® -1 cloning vector. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Aphid biology: Expressed genes from alate Toxoptera citricida, the brown citrus aphid
    Article Snippet: Plasmid DNA was extracted using the Qiagen 9600 liquid handling robot and the QIAprep 96 Turbo miniprep kit according to the recommended protocol (QIAGEN, www.quigen.com ). .. Sequencing reactions were performed using the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, home.appliedbiosystems.com ) along with a universal T3 primer.

    Software:

    Article Title: Genetic variants of HvCbf14 are statistically associated with frost tolerance in a European germplasm collection of Hordeum vulgare
    Article Snippet: PCR products were sequenced on both strands using ABI PRISM BigDye Primer Cycle sequencing kit v.3.1 (Applied Biosystems, Foster City, USA), following manufacturer’s instruction. .. PCR products were sequenced on both strands using ABI PRISM BigDye Primer Cycle sequencing kit v.3.1 (Applied Biosystems, Foster City, USA), following manufacturer’s instruction.

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  • 99
    Thermo Fisher abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing ready reaction kit/product/Thermo Fisher
    Average 99 stars, based on 147 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator cycle sequencing ready reaction kit - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

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    Thermo Fisher abi prism bigdye terminator v3 1 cycle sequencing kit
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    https://www.bioz.com/result/abi prism bigdye terminator v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 114 article reviews
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    Thermo Fisher abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit
    Abi Prism Bigdye Terminator V3 0 Ready Reaction Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator v3 0 ready reaction cycle sequencing kit/product/Thermo Fisher
    Average 97 stars, based on 15 article reviews
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