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PerkinElmer abi prism bigdye primer cycle sequencing kit
Abi Prism Bigdye Primer Cycle Sequencing Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/abi prism bigdye primer cycle sequencing kit/product/PerkinElmer
Average 77 stars, based on 3 article reviews
Price from $9.99 to $1999.99
abi prism bigdye primer cycle sequencing kit - by Bioz Stars, 2020-02
77/100 stars

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Ligation:

Article Title: Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering
Article Snippet: Standard recombinant DNA manipulation techniques were used for isolation of plasmid DNA, restriction digestion, ligation, and transformation into E. coli ( ). .. DNA was sequenced by cycle sequencing by using an ABI PRISM BigDye Primer cycle sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.).

Isolation:

Article Title: Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering
Article Snippet: Standard recombinant DNA manipulation techniques were used for isolation of plasmid DNA, restriction digestion, ligation, and transformation into E. coli ( ). .. DNA was sequenced by cycle sequencing by using an ABI PRISM BigDye Primer cycle sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.).

Subcloning:

Article Title: Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering
Article Snippet: DNA fragments required for subcloning experiments were gel purified with a QIAGEN kit (QIAGEN, Hilden, Germany). .. DNA was sequenced by cycle sequencing by using an ABI PRISM BigDye Primer cycle sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.).

Purification:

Article Title: Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering
Article Snippet: DNA fragments required for subcloning experiments were gel purified with a QIAGEN kit (QIAGEN, Hilden, Germany). .. DNA was sequenced by cycle sequencing by using an ABI PRISM BigDye Primer cycle sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.).

Sequencing:

Article Title: Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering
Article Snippet: .. DNA was sequenced by cycle sequencing by using an ABI PRISM BigDye Primer cycle sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.). .. Plasmids pTA3 and pTA13, which were isolated from the second random mutant library, were derivatives of plasmid pSTV28 (Takara Shuzo) containing the evolved phospholipase A1 genes.

Article Title: Identification of the tliDEF ABC Transporter Specific for Lipase in Pseudomonas fluorescens SIK W1
Article Snippet: .. DNA sequences were determined by cycle sequencing with the ABI PRISM BigDye primer cycle-sequencing kit with AmpliTaq DNA polymerase (Perkin-Elmer Corp., Norwalk, Conn.). .. Synthetic oligonucleotides were used as primers to sequence both strands.

Transformation Assay:

Article Title: Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering
Article Snippet: Standard recombinant DNA manipulation techniques were used for isolation of plasmid DNA, restriction digestion, ligation, and transformation into E. coli ( ). .. DNA was sequenced by cycle sequencing by using an ABI PRISM BigDye Primer cycle sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.).

Recombinant:

Article Title: Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering
Article Snippet: Standard recombinant DNA manipulation techniques were used for isolation of plasmid DNA, restriction digestion, ligation, and transformation into E. coli ( ). .. DNA was sequenced by cycle sequencing by using an ABI PRISM BigDye Primer cycle sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.).

Plasmid Preparation:

Article Title: Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering
Article Snippet: Standard recombinant DNA manipulation techniques were used for isolation of plasmid DNA, restriction digestion, ligation, and transformation into E. coli ( ). .. DNA was sequenced by cycle sequencing by using an ABI PRISM BigDye Primer cycle sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.).

Article Title: Identification of the tliDEF ABC Transporter Specific for Lipase in Pseudomonas fluorescens SIK W1
Article Snippet: DNA sequences were determined by cycle sequencing with the ABI PRISM BigDye primer cycle-sequencing kit with AmpliTaq DNA polymerase (Perkin-Elmer Corp., Norwalk, Conn.). .. Nucleotide and amino acid sequence data were analyzed by using DNASIS (Hitachi Software Engineering) and Vector NTI (Informax, Inc.).

Software:

Article Title: Identification of the tliDEF ABC Transporter Specific for Lipase in Pseudomonas fluorescens SIK W1
Article Snippet: DNA sequences were determined by cycle sequencing with the ABI PRISM BigDye primer cycle-sequencing kit with AmpliTaq DNA polymerase (Perkin-Elmer Corp., Norwalk, Conn.). .. Nucleotide and amino acid sequence data were analyzed by using DNASIS (Hitachi Software Engineering) and Vector NTI (Informax, Inc.).

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    PerkinElmer abi prism bigdye primer cycle sequencing kit
    Abi Prism Bigdye Primer Cycle Sequencing Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye primer cycle sequencing kit/product/PerkinElmer
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye primer cycle sequencing kit - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    90
    PerkinElmer abi prism bigdye terminator cycle sequencing ready reaction kit
    Abi Prism Bigdye Terminator Cycle Sequencing Ready Reaction Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism bigdye terminator cycle sequencing ready reaction kit/product/PerkinElmer
    Average 90 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    abi prism bigdye terminator cycle sequencing ready reaction kit - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

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