abi prism 377 dna sequencer  (Thermo Fisher)


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    Thermo Fisher abi prism 377 dna sequencer
    Abi Prism 377 Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism 377 dna sequencer/product/Thermo Fisher
    Average 99 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    abi prism 377 dna sequencer - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.). .. Moreover, PCR products were cloned with a pGEM-T Vector System (Promega) and recombinant plasmids were sequenced.

    Article Title: Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses
    Article Snippet: All protocols used to generate cloning and baculovirus transfer vectors were completed by standard procedures ( ) or according to the instructions of the manufacturer. .. To confirm the orientation and sequence of the capsid gene in pBac-CV186 and pBac-JN, the capsid gene was sequenced with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, Calif.).

    Article Title: Characterization of the Two Mycobacterium tuberculosis recA Promoters
    Article Snippet: For each clone or mutant made, the sequences of the promoter region and the junctions to the vector were determined on an ABI Prism 377 DNA sequencer using the ABI Prism dRhodamine dye terminator cycle sequencing kit (PE Applied Biosystems). .. Clones were introduced into M. smegmatis or M. tuberculosis by electroporation ( ) and verified by PCR and sequencing as described previously ( ).

    Article Title: Origin and Evolution of Processed Pseudogenes That Stabilize Functional Makorin1 mRNAs in Mice, Primates and Other Mammals
    Article Snippet: Cloned products were purified with QIAprep Spin Miniprep (QIAGEN) and used as templates in subsequent sequencing reactions. .. Sequencing reactions were performed with BigDye Terminator v1.1 cycle sequencing kits (Applied Biosystems, Foster City, CA) and analyzed on an ABI PRISM 377 DNA sequencer (Applied Biosystems).

    Amplification:

    Article Title: Deletions in the Y-derived amelogenin gene fragment in the Indian population
    Article Snippet: .. Y-STR amplification was carried out in a single tube multiplex reaction [ ] and genotyped on an ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems, Foster City, CA). ..

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: A dilution of the original PCR was reamplified using 5′ RACE Ambriged universal amplification primer and the nested primer e2R (5′-ATC TTC AGC CAT TTT GAT TTC TTC AAT CC-3′) complementary to an exon 2 sequence. .. PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.).

    Article Title: Activities of Fluoroquinolones against Streptococcus pneumoniae Type II Topoisomerases Purified as Recombinant Proteins
    Article Snippet: .. Amplified templates for the quinolone resistance-determining region (QRDR) were sequenced by using an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.) with Big Dye terminator chemistry. .. The oligonucleotides used to prime the DNA sequencing reactions for sequencing of the QRDRs are described in Table .

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: The resulting cDNAs were amplified by PCR using fluorescence-labeled primers 3a and 6b. .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Imported Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Clones in a Greek Hospital: Impact of Infection Control Measures for Restraining Their Dissemination
    Article Snippet: K. pneumoniae isolates were screened for β-lactamase genes by PCR amplification using a panel of primers for the detection of MBLs, KPCs , OXA-48 , plasmid-mediated AmpC-encoding genes in a single PCR for each gene , and ESBLs, including SHV, TEM, CTX-M, and IBC/GES enzymes ( ). .. The PCR products which were subjected to direct sequencing were purified using ExoSAP-IT reagent (USB Corporation, Cleveland, OH) and used as templates for sequencing on both strands with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA).

    Article Title: Effect of Anthropogenic Landscape Features on Population Genetic Differentiation of Przewalski's Gazelle: Main Role of Human Settlement
    Article Snippet: Paragraph title: DNA extraction and microsatellite amplification ... PCR products were resolved on an ABI PRISM 377 DNA Sequencer (Applied Biosystems).

    Article Title: Origin and Evolution of Processed Pseudogenes That Stabilize Functional Makorin1 mRNAs in Mice, Primates and Other Mammals
    Article Snippet: Paragraph title: Genomic DNA sources and PCR amplification: ... Sequencing reactions were performed with BigDye Terminator v1.1 cycle sequencing kits (Applied Biosystems, Foster City, CA) and analyzed on an ABI PRISM 377 DNA sequencer (Applied Biosystems).

    Article Title: Novel link between prostaglandin E2 (PGE2) and cholinergic signaling in lung cancer: The role of c-Jun in PGE2-induced α7 nicotinic acetylcholine receptor expression and tumor cell proliferation
    Article Snippet: The amplification products were then digested using Dpn I restriction enzyme and transformed into XL1-Blue Supercompetent cells. .. Colonies were selected and screened for mutants by sequencing using ABI Prism 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Synthesized:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: Total RNA (1 μg), purified from human adult skeletal muscle or from RD cells, was subjected to 5′ rapid amplification of cDNA ends (RACE) by the use of a 5′ RACE system (Life Technologies, Gaithersburg, Md.). cDNA was synthesized using the primer e5R, complementary to an exon 5 sequence of the AβH-J-J locus (Fig. and ) , and a poly(G) tail was added by terminal transferase. .. PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.).

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: The internal standard RNA was synthesized in vitro with T7 RNA polymerase using modified aromatase cDNA as a template. .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Novel link between prostaglandin E2 (PGE2) and cholinergic signaling in lung cancer: The role of c-Jun in PGE2-induced α7 nicotinic acetylcholine receptor expression and tumor cell proliferation
    Article Snippet: Site-directed mutagenesis To prepare the site-directed mutant of the mouse α7 nAChR gene promoter, the following complimentary oligonucleotide primers were synthesized: mutated c-Jun (-455/-449 bp), 5′-GAAAGTGAGACtaggaGGCTTTCTGCTG-3′ (forward), 5′-CAGCAGAAAGCCtcctaGTCTCACTTTC-3′ (reverse). .. Colonies were selected and screened for mutants by sequencing using ABI Prism 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    TA Cloning:

    Article Title: Origin and Evolution of Processed Pseudogenes That Stabilize Functional Makorin1 mRNAs in Mice, Primates and Other Mammals
    Article Snippet: Purified products were either sequenced directly or cloned into pCR2.1 TOPO with TOPO TA cloning kits (Invitrogen, San Diego). .. Sequencing reactions were performed with BigDye Terminator v1.1 cycle sequencing kits (Applied Biosystems, Foster City, CA) and analyzed on an ABI PRISM 377 DNA sequencer (Applied Biosystems).

    Construct:

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: To prepare the internal standard RNA, modified human aromatase cDNA was constructed by inserting a 21-base pair DNA fragment between the two PCR primer sites. .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Novel link between prostaglandin E2 (PGE2) and cholinergic signaling in lung cancer: The role of c-Jun in PGE2-induced α7 nicotinic acetylcholine receptor expression and tumor cell proliferation
    Article Snippet: The α7 nAChR gene promoter construct containing the specific site-directed deletion mutation of c-Jun binding site was generated using the QuikChange II Site-Directed Mutagenese Kit according to the manufacturer's recommendations (Stratagene, La Jolla, CA, USA). .. Colonies were selected and screened for mutants by sequencing using ABI Prism 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Genus Paracoccidioides: Species Recognition and Biogeographic Aspects
    Article Snippet: One microliter of each sample was loaded onto 12% polyacrylamide gel and submitted to electrophoresis in an ABI PRISM 377 DNA sequencer (Applied Biosystems). .. For Real Time PCR, TaqMan probes, as well as forward and reverse primers for GP43 , ARF and PRP8 intein, were designed ( ).

    Modification:

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: The internal standard RNA was synthesized in vitro with T7 RNA polymerase using modified aromatase cDNA as a template. .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Transformation Assay:

    Article Title: Novel link between prostaglandin E2 (PGE2) and cholinergic signaling in lung cancer: The role of c-Jun in PGE2-induced α7 nicotinic acetylcholine receptor expression and tumor cell proliferation
    Article Snippet: The amplification products were then digested using Dpn I restriction enzyme and transformed into XL1-Blue Supercompetent cells. .. Colonies were selected and screened for mutants by sequencing using ABI Prism 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Electron Microscopy:

    Article Title: Infective Meningitis Caused by Phialemonium curvatum
    Article Snippet: Scanning electron microscopy (SEM) images revealed that the shape of the conidia was either allantoid or obovate ( ). .. The sizes of the PCR products were estimated using a standard DNA ladder and sequenced with an automated ABI Prism 377 DNA sequencer (Applied Biosystems Corp., Foster City, CA).

    Generated:

    Article Title: Novel link between prostaglandin E2 (PGE2) and cholinergic signaling in lung cancer: The role of c-Jun in PGE2-induced α7 nicotinic acetylcholine receptor expression and tumor cell proliferation
    Article Snippet: The α7 nAChR gene promoter construct containing the specific site-directed deletion mutation of c-Jun binding site was generated using the QuikChange II Site-Directed Mutagenese Kit according to the manufacturer's recommendations (Stratagene, La Jolla, CA, USA). .. Colonies were selected and screened for mutants by sequencing using ABI Prism 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: .. PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.). .. Moreover, PCR products were cloned with a pGEM-T Vector System (Promega) and recombinant plasmids were sequenced.

    Article Title: Activities of Fluoroquinolones against Streptococcus pneumoniae Type II Topoisomerases Purified as Recombinant Proteins
    Article Snippet: The PCR products were purified with Qiagen Q1A quick spin columns. .. Amplified templates for the quinolone resistance-determining region (QRDR) were sequenced by using an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.) with Big Dye terminator chemistry.

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genus Paracoccidioides: Species Recognition and Biogeographic Aspects
    Article Snippet: SNaPshot reactions were carried out as follows: 6 µL of ultra pure water, 1 µL of ½ TERM buffer (200 mMTrisHCl, 5 mM MgCl2, pH 9.0), 1 µL of SNaPmix (Applied Biosystems), 1 µL of SNP-primer (1 pmol/µL) and 1 µL of the PCR product, previously purified with ExoSAP-IT (GE Healthcare) according to the supplier's instructions. .. One microliter of each sample was loaded onto 12% polyacrylamide gel and submitted to electrophoresis in an ABI PRISM 377 DNA sequencer (Applied Biosystems).

    Article Title: Imported Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Clones in a Greek Hospital: Impact of Infection Control Measures for Restraining Their Dissemination
    Article Snippet: .. The PCR products which were subjected to direct sequencing were purified using ExoSAP-IT reagent (USB Corporation, Cleveland, OH) and used as templates for sequencing on both strands with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA). .. In order to determine the clonal relationships of the K. pneumoniae isolates, pulsed-field gel electrophoresis (PFGE) of the XbaI-digested genomic DNA was performed using a CHEF-DRIII system (Bio-Rad, Hemel Hempstead, United Kingdom), with a running time of 21 h and pulse times ranging from 3 to 40 s. The PFGE patterns were compared visually according to previously described criteria ( ).

    Article Title: Usher syndrome IIIA gene clarin-1 is essential for hair cell function and associated neural activation †
    Article Snippet: The following PCR conditions were used: 94°C for 2 min followed by 34 cycles of 30 s each, 55°C for 30 s and 72°C for 1 min. All PCR products were resolved on 3% low range ultra agarose gels (BIO-RAD) and stained with 5% ethidium bromide. .. The ABI Prism 377 DNA sequencer (Applied Biosystems) was used to analyze and display the resulting sequence data.

    Article Title: Effect of Anthropogenic Landscape Features on Population Genetic Differentiation of Przewalski's Gazelle: Main Role of Human Settlement
    Article Snippet: .. PCR products were resolved on an ABI PRISM 377 DNA Sequencer (Applied Biosystems). .. Alleles were scored using GENESCAN version 3.7 (Applied Biosystems) and GeneMarker version 1.71 (SoftGenetics).

    Article Title: Infective Meningitis Caused by Phialemonium curvatum
    Article Snippet: .. The sizes of the PCR products were estimated using a standard DNA ladder and sequenced with an automated ABI Prism 377 DNA sequencer (Applied Biosystems Corp., Foster City, CA). .. Then, the sequences were analyzed by nucleotide-nucleotide basic local alignment search tool (BLAST) searches and DNAMAN 5.2 (Lynnon Biosoft, Pointe-Claire, Quebec).

    Article Title: Characterization of the Two Mycobacterium tuberculosis recA Promoters
    Article Snippet: For each clone or mutant made, the sequences of the promoter region and the junctions to the vector were determined on an ABI Prism 377 DNA sequencer using the ABI Prism dRhodamine dye terminator cycle sequencing kit (PE Applied Biosystems). .. Clones were introduced into M. smegmatis or M. tuberculosis by electroporation ( ) and verified by PCR and sequencing as described previously ( ).

    Article Title: Origin and Evolution of Processed Pseudogenes That Stabilize Functional Makorin1 mRNAs in Mice, Primates and Other Mammals
    Article Snippet: Paragraph title: Genomic DNA sources and PCR amplification: ... Sequencing reactions were performed with BigDye Terminator v1.1 cycle sequencing kits (Applied Biosystems, Foster City, CA) and analyzed on an ABI PRISM 377 DNA sequencer (Applied Biosystems).

    DNA Sequencing:

    Article Title: Activities of Fluoroquinolones against Streptococcus pneumoniae Type II Topoisomerases Purified as Recombinant Proteins
    Article Snippet: Amplified templates for the quinolone resistance-determining region (QRDR) were sequenced by using an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.) with Big Dye terminator chemistry. .. The oligonucleotides used to prime the DNA sequencing reactions for sequencing of the QRDRs are described in Table .

    Sequencing:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: .. PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.). .. Moreover, PCR products were cloned with a pGEM-T Vector System (Promega) and recombinant plasmids were sequenced.

    Article Title: Activities of Fluoroquinolones against Streptococcus pneumoniae Type II Topoisomerases Purified as Recombinant Proteins
    Article Snippet: Paragraph title: Sequencing of topoisomerase gene QRDRs. ... Amplified templates for the quinolone resistance-determining region (QRDR) were sequenced by using an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.) with Big Dye terminator chemistry.

    Article Title: Imported Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Clones in a Greek Hospital: Impact of Infection Control Measures for Restraining Their Dissemination
    Article Snippet: .. The PCR products which were subjected to direct sequencing were purified using ExoSAP-IT reagent (USB Corporation, Cleveland, OH) and used as templates for sequencing on both strands with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA). .. In order to determine the clonal relationships of the K. pneumoniae isolates, pulsed-field gel electrophoresis (PFGE) of the XbaI-digested genomic DNA was performed using a CHEF-DRIII system (Bio-Rad, Hemel Hempstead, United Kingdom), with a running time of 21 h and pulse times ranging from 3 to 40 s. The PFGE patterns were compared visually according to previously described criteria ( ).

    Article Title: Usher syndrome IIIA gene clarin-1 is essential for hair cell function and associated neural activation †
    Article Snippet: .. The ABI Prism 377 DNA sequencer (Applied Biosystems) was used to analyze and display the resulting sequence data. ..

    Article Title: Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses
    Article Snippet: .. To confirm the orientation and sequence of the capsid gene in pBac-CV186 and pBac-JN, the capsid gene was sequenced with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, Calif.). .. pBac-CV186 and pBac-JN were transfected into Sf9 cells with a Bac-N-Blue Transfection kit (Invitrogen), according to the instructions of the manufacturer.

    Article Title: Characterization of the Two Mycobacterium tuberculosis recA Promoters
    Article Snippet: .. For each clone or mutant made, the sequences of the promoter region and the junctions to the vector were determined on an ABI Prism 377 DNA sequencer using the ABI Prism dRhodamine dye terminator cycle sequencing kit (PE Applied Biosystems). .. Clones were introduced into M. smegmatis or M. tuberculosis by electroporation ( ) and verified by PCR and sequencing as described previously ( ).

    Article Title: Origin and Evolution of Processed Pseudogenes That Stabilize Functional Makorin1 mRNAs in Mice, Primates and Other Mammals
    Article Snippet: .. Sequencing reactions were performed with BigDye Terminator v1.1 cycle sequencing kits (Applied Biosystems, Foster City, CA) and analyzed on an ABI PRISM 377 DNA sequencer (Applied Biosystems). .. To minimize sequencing errors, PCR primary products or cloned DNAs were sequenced two to six times in both directions.

    Article Title: Amplicon Sequencing and Improved Detection of Human Rhinovirus in Respiratory Samples
    Article Snippet: .. Sequencing was performed with 5 μl of the diluted amplicons, 1 pmol of each primer (the forward and reverse primers used for the HRV RT-PCR) per μl, and the DYEnamic ET Terminator Cycle Sequencing kit (Amersham Biosciences) on an ABI Prism 377 DNA Sequencer (Applied Biosystems, PE Europe B.V.), according to the manufacturer's instructions (25 cycles of 10 s at 95°C and 3 min at 60°C, with the reaction mixture then held at 4°C). .. Sequence alignment was performed with the ClustalW program, and phylogenetic analyses were carried out with the Phylip package by using the Seqboot (100 replicates), the Dnadist, and the neighbor-joining programs ( ).

    Article Title: Novel link between prostaglandin E2 (PGE2) and cholinergic signaling in lung cancer: The role of c-Jun in PGE2-induced α7 nicotinic acetylcholine receptor expression and tumor cell proliferation
    Article Snippet: .. Colonies were selected and screened for mutants by sequencing using ABI Prism 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA). .. Transient transfection assay The 947-, 621-, 422-, and 65-bp mouse α7 nAChR promoter deletion constructs (pGL3-α7LUC) ligated to the luciferase reporter gene were a gift from Dr. Stitzel at the University of Colorado and have been reported previously.

    Article Title: Analysis of Bordetella pertussis pertactin and pertussis toxin types from Queensland, Australia, 1999-2003
    Article Snippet: .. Cycle sequencing reaction products were purified using sodium acetate/ethanol precipitation, and subsequently analysed on an ABI PRISM 377 DNA Sequencer (Applied Biosystems). .. Sequences were assembled and analysed using Sequencher 3.0 software (Gene Codes Corporation).

    Recombinant:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.). .. Moreover, PCR products were cloned with a pGEM-T Vector System (Promega) and recombinant plasmids were sequenced.

    Article Title: Characterization of the Two Mycobacterium tuberculosis recA Promoters
    Article Snippet: Paragraph title: Recombinant-DNA techniques. ... For each clone or mutant made, the sequences of the promoter region and the junctions to the vector were determined on an ABI Prism 377 DNA sequencer using the ABI Prism dRhodamine dye terminator cycle sequencing kit (PE Applied Biosystems).

    Transmission Electron Microscopy:

    Article Title: Imported Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Clones in a Greek Hospital: Impact of Infection Control Measures for Restraining Their Dissemination
    Article Snippet: K. pneumoniae isolates were screened for β-lactamase genes by PCR amplification using a panel of primers for the detection of MBLs, KPCs , OXA-48 , plasmid-mediated AmpC-encoding genes in a single PCR for each gene , and ESBLs, including SHV, TEM, CTX-M, and IBC/GES enzymes ( ). .. The PCR products which were subjected to direct sequencing were purified using ExoSAP-IT reagent (USB Corporation, Cleveland, OH) and used as templates for sequencing on both strands with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA).

    DNA Extraction:

    Article Title: Imported Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Clones in a Greek Hospital: Impact of Infection Control Measures for Restraining Their Dissemination
    Article Snippet: DNA was extracted from overnight cultures using the Wizard DNA extraction kit (Promega, Madison, WI). .. The PCR products which were subjected to direct sequencing were purified using ExoSAP-IT reagent (USB Corporation, Cleveland, OH) and used as templates for sequencing on both strands with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA).

    Article Title: Effect of Anthropogenic Landscape Features on Population Genetic Differentiation of Przewalski's Gazelle: Main Role of Human Settlement
    Article Snippet: Paragraph title: DNA extraction and microsatellite amplification ... PCR products were resolved on an ABI PRISM 377 DNA Sequencer (Applied Biosystems).

    Fluorescence:

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: The resulting cDNAs were amplified by PCR using fluorescence-labeled primers 3a and 6b. .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Electroporation:

    Article Title: Characterization of the Two Mycobacterium tuberculosis recA Promoters
    Article Snippet: For each clone or mutant made, the sequences of the promoter region and the junctions to the vector were determined on an ABI Prism 377 DNA sequencer using the ABI Prism dRhodamine dye terminator cycle sequencing kit (PE Applied Biosystems). .. Clones were introduced into M. smegmatis or M. tuberculosis by electroporation ( ) and verified by PCR and sequencing as described previously ( ).

    Mutagenesis:

    Article Title: Characterization of the Two Mycobacterium tuberculosis recA Promoters
    Article Snippet: .. For each clone or mutant made, the sequences of the promoter region and the junctions to the vector were determined on an ABI Prism 377 DNA sequencer using the ABI Prism dRhodamine dye terminator cycle sequencing kit (PE Applied Biosystems). .. Clones were introduced into M. smegmatis or M. tuberculosis by electroporation ( ) and verified by PCR and sequencing as described previously ( ).

    Article Title: Novel link between prostaglandin E2 (PGE2) and cholinergic signaling in lung cancer: The role of c-Jun in PGE2-induced α7 nicotinic acetylcholine receptor expression and tumor cell proliferation
    Article Snippet: Paragraph title: Site-directed mutagenesis ... Colonies were selected and screened for mutants by sequencing using ABI Prism 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Subcloning:

    Article Title: Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses
    Article Snippet: Paragraph title: Subcloning of capsid genes in baculovirus transfer vectors and capsid gene sequencing. ... To confirm the orientation and sequence of the capsid gene in pBac-CV186 and pBac-JN, the capsid gene was sequenced with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, Calif.).

    Size-exclusion Chromatography:

    Article Title: Genus Paracoccidioides: Species Recognition and Biogeographic Aspects
    Article Snippet: Reactions were performed in a Whatman Biometra® (T Gradient) thermocycler using the following conditions: 40 cycles 95°C for 10 sec, 50°C for 5 sec and 60°C for 30 sec. .. One microliter of each sample was loaded onto 12% polyacrylamide gel and submitted to electrophoresis in an ABI PRISM 377 DNA sequencer (Applied Biosystems).

    Labeling:

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: The sense primer 3a for PCR was labeled with a fluorescent dye, 6-carboxyfluorescein (PerkinElmer, Waltham, MA, USA). .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Effect of Anthropogenic Landscape Features on Population Genetic Differentiation of Przewalski's Gazelle: Main Role of Human Settlement
    Article Snippet: Then, forward primers of the 13 markers were fluorescently labeled with FAM, HEX and TAMRA. .. PCR products were resolved on an ABI PRISM 377 DNA Sequencer (Applied Biosystems).

    Purification:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: .. PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.). .. Moreover, PCR products were cloned with a pGEM-T Vector System (Promega) and recombinant plasmids were sequenced.

    Article Title: Activities of Fluoroquinolones against Streptococcus pneumoniae Type II Topoisomerases Purified as Recombinant Proteins
    Article Snippet: The PCR products were purified with Qiagen Q1A quick spin columns. .. Amplified templates for the quinolone resistance-determining region (QRDR) were sequenced by using an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.) with Big Dye terminator chemistry.

    Article Title: Genus Paracoccidioides: Species Recognition and Biogeographic Aspects
    Article Snippet: SNaPshot reactions were carried out as follows: 6 µL of ultra pure water, 1 µL of ½ TERM buffer (200 mMTrisHCl, 5 mM MgCl2, pH 9.0), 1 µL of SNaPmix (Applied Biosystems), 1 µL of SNP-primer (1 pmol/µL) and 1 µL of the PCR product, previously purified with ExoSAP-IT (GE Healthcare) according to the supplier's instructions. .. One microliter of each sample was loaded onto 12% polyacrylamide gel and submitted to electrophoresis in an ABI PRISM 377 DNA sequencer (Applied Biosystems).

    Article Title: Imported Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Clones in a Greek Hospital: Impact of Infection Control Measures for Restraining Their Dissemination
    Article Snippet: .. The PCR products which were subjected to direct sequencing were purified using ExoSAP-IT reagent (USB Corporation, Cleveland, OH) and used as templates for sequencing on both strands with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA). .. In order to determine the clonal relationships of the K. pneumoniae isolates, pulsed-field gel electrophoresis (PFGE) of the XbaI-digested genomic DNA was performed using a CHEF-DRIII system (Bio-Rad, Hemel Hempstead, United Kingdom), with a running time of 21 h and pulse times ranging from 3 to 40 s. The PFGE patterns were compared visually according to previously described criteria ( ).

    Article Title: Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses
    Article Snippet: All RT-PCR products and plasmid DNA were purified with a QIAquick gel extraction kit (Qiagen). .. To confirm the orientation and sequence of the capsid gene in pBac-CV186 and pBac-JN, the capsid gene was sequenced with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, Calif.).

    Article Title: Origin and Evolution of Processed Pseudogenes That Stabilize Functional Makorin1 mRNAs in Mice, Primates and Other Mammals
    Article Snippet: Cloned products were purified with QIAprep Spin Miniprep (QIAGEN) and used as templates in subsequent sequencing reactions. .. Sequencing reactions were performed with BigDye Terminator v1.1 cycle sequencing kits (Applied Biosystems, Foster City, CA) and analyzed on an ABI PRISM 377 DNA sequencer (Applied Biosystems).

    Article Title: Analysis of Bordetella pertussis pertactin and pertussis toxin types from Queensland, Australia, 1999-2003
    Article Snippet: .. Cycle sequencing reaction products were purified using sodium acetate/ethanol precipitation, and subsequently analysed on an ABI PRISM 377 DNA Sequencer (Applied Biosystems). .. Sequences were assembled and analysed using Sequencher 3.0 software (Gene Codes Corporation).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Usher syndrome IIIA gene clarin-1 is essential for hair cell function and associated neural activation †
    Article Snippet: Sequences of RT–PCR products were determined with BigDye Terminator Cycle sequencing reagents and protocols (Applied Biosystems, CA). .. The ABI Prism 377 DNA sequencer (Applied Biosystems) was used to analyze and display the resulting sequence data.

    Article Title: Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses
    Article Snippet: All RT-PCR products and plasmid DNA were purified with a QIAquick gel extraction kit (Qiagen). .. To confirm the orientation and sequence of the capsid gene in pBac-CV186 and pBac-JN, the capsid gene was sequenced with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, Calif.).

    Article Title: Amplicon Sequencing and Improved Detection of Human Rhinovirus in Respiratory Samples
    Article Snippet: .. Sequencing was performed with 5 μl of the diluted amplicons, 1 pmol of each primer (the forward and reverse primers used for the HRV RT-PCR) per μl, and the DYEnamic ET Terminator Cycle Sequencing kit (Amersham Biosciences) on an ABI Prism 377 DNA Sequencer (Applied Biosystems, PE Europe B.V.), according to the manufacturer's instructions (25 cycles of 10 s at 95°C and 3 min at 60°C, with the reaction mixture then held at 4°C). .. Sequence alignment was performed with the ClustalW program, and phylogenetic analyses were carried out with the Phylip package by using the Seqboot (100 replicates), the Dnadist, and the neighbor-joining programs ( ).

    Staining:

    Article Title: Usher syndrome IIIA gene clarin-1 is essential for hair cell function and associated neural activation †
    Article Snippet: The following PCR conditions were used: 94°C for 2 min followed by 34 cycles of 30 s each, 55°C for 30 s and 72°C for 1 min. All PCR products were resolved on 3% low range ultra agarose gels (BIO-RAD) and stained with 5% ethidium bromide. .. The ABI Prism 377 DNA sequencer (Applied Biosystems) was used to analyze and display the resulting sequence data.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: A dilution of the original PCR was reamplified using 5′ RACE Ambriged universal amplification primer and the nested primer e2R (5′-ATC TTC AGC CAT TTT GAT TTC TTC AAT CC-3′) complementary to an exon 2 sequence. .. PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.).

    Rapid Amplification of cDNA Ends:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: Total RNA (1 μg), purified from human adult skeletal muscle or from RD cells, was subjected to 5′ rapid amplification of cDNA ends (RACE) by the use of a 5′ RACE system (Life Technologies, Gaithersburg, Md.). cDNA was synthesized using the primer e5R, complementary to an exon 5 sequence of the AβH-J-J locus (Fig. and ) , and a poly(G) tail was added by terminal transferase. .. PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.).

    Plasmid Preparation:

    Article Title: Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A?H-J-J Locus, Encoding Aspartyl-?-Hydroxylase, Junctin, and Junctate
    Article Snippet: PCR products were purified with Microcon-30 (Millipore, Bedford, Mass.) and subjected to direct sequencing with an ABI PRISM BigDye terminator cycle sequencing ready reaction kit and an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.). .. Moreover, PCR products were cloned with a pGEM-T Vector System (Promega) and recombinant plasmids were sequenced.

    Article Title: Imported Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae Clones in a Greek Hospital: Impact of Infection Control Measures for Restraining Their Dissemination
    Article Snippet: K. pneumoniae isolates were screened for β-lactamase genes by PCR amplification using a panel of primers for the detection of MBLs, KPCs , OXA-48 , plasmid-mediated AmpC-encoding genes in a single PCR for each gene , and ESBLs, including SHV, TEM, CTX-M, and IBC/GES enzymes ( ). .. The PCR products which were subjected to direct sequencing were purified using ExoSAP-IT reagent (USB Corporation, Cleveland, OH) and used as templates for sequencing on both strands with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA).

    Article Title: Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses
    Article Snippet: All RT-PCR products and plasmid DNA were purified with a QIAquick gel extraction kit (Qiagen). .. To confirm the orientation and sequence of the capsid gene in pBac-CV186 and pBac-JN, the capsid gene was sequenced with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, Calif.).

    Article Title: Characterization of the Two Mycobacterium tuberculosis recA Promoters
    Article Snippet: .. For each clone or mutant made, the sequences of the promoter region and the junctions to the vector were determined on an ABI Prism 377 DNA sequencer using the ABI Prism dRhodamine dye terminator cycle sequencing kit (PE Applied Biosystems). .. Clones were introduced into M. smegmatis or M. tuberculosis by electroporation ( ) and verified by PCR and sequencing as described previously ( ).

    Software:

    Article Title: Analysis of Bordetella pertussis pertactin and pertussis toxin types from Queensland, Australia, 1999-2003
    Article Snippet: Cycle sequencing reaction products were purified using sodium acetate/ethanol precipitation, and subsequently analysed on an ABI PRISM 377 DNA Sequencer (Applied Biosystems). .. Sequences were assembled and analysed using Sequencher 3.0 software (Gene Codes Corporation).

    Electrophoresis:

    Article Title: Genus Paracoccidioides: Species Recognition and Biogeographic Aspects
    Article Snippet: .. One microliter of each sample was loaded onto 12% polyacrylamide gel and submitted to electrophoresis in an ABI PRISM 377 DNA sequencer (Applied Biosystems). ..

    Multiplex Assay:

    Article Title: Deletions in the Y-derived amelogenin gene fragment in the Indian population
    Article Snippet: .. Y-STR amplification was carried out in a single tube multiplex reaction [ ] and genotyped on an ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems, Foster City, CA). ..

    Binding Assay:

    Article Title: Novel link between prostaglandin E2 (PGE2) and cholinergic signaling in lung cancer: The role of c-Jun in PGE2-induced α7 nicotinic acetylcholine receptor expression and tumor cell proliferation
    Article Snippet: The α7 nAChR gene promoter construct containing the specific site-directed deletion mutation of c-Jun binding site was generated using the QuikChange II Site-Directed Mutagenese Kit according to the manufacturer's recommendations (Stratagene, La Jolla, CA, USA). .. Colonies were selected and screened for mutants by sequencing using ABI Prism 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Agarose Gel Electrophoresis:

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA). ..

    In Vitro:

    Article Title: Alternative use of multiple exons 1 of aromatase gene in cancerous and normal breast tissues from women over the age of 80 years
    Article Snippet: The internal standard RNA was synthesized in vitro with T7 RNA polymerase using modified aromatase cDNA as a template. .. The fluorescent PCR products were electrophoresed in a 2% agarose gel and analyzed with an ABI PRISM 377 DNA Sequencer (Applied Biosystems, Foster City, CA, USA).

    Gel Extraction:

    Article Title: Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses
    Article Snippet: All RT-PCR products and plasmid DNA were purified with a QIAquick gel extraction kit (Qiagen). .. To confirm the orientation and sequence of the capsid gene in pBac-CV186 and pBac-JN, the capsid gene was sequenced with an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, Calif.).

    Article Title: Origin and Evolution of Processed Pseudogenes That Stabilize Functional Makorin1 mRNAs in Mice, Primates and Other Mammals
    Article Snippet: The PCR products were purified using QIAquick PCR purification kits (QIAGEN, Valencia, CA), QIAquick gel extraction kits (QIAGEN), or ExoSAP-ITs (United States Biochemical, Cleveland). .. Sequencing reactions were performed with BigDye Terminator v1.1 cycle sequencing kits (Applied Biosystems, Foster City, CA) and analyzed on an ABI PRISM 377 DNA sequencer (Applied Biosystems).

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    Thermo Fisher abi prism 377 automated dna sequencer
    Abi Prism 377 Automated Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher abi prism 377 dna sequencer
    Abi Prism 377 Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism 377 dna sequencer/product/Thermo Fisher
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    Price from $9.99 to $1999.99
    abi prism 377 dna sequencer - by Bioz Stars, 2020-02
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