abi prism 377 dna sequencer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Thermo Fisher abi prism 377 dna sequencer
    <t>DNA</t> cycle sequencing using an extendable primer . Panel A contains the design of an EOs and TOs for the sequencing of a region of the Escherichia coli streptomycin operon. Vertical bars indicate regions of clamp region of hybridisation between the EO and TO. Capital letters show actual sequence of the oligonucleotides and small, underlined letters indicate the extended region of the EO. N indicates a position with either A, T, G or C and S indicates a position with either G or C nucleotide. Panel B shows an electropherogram of a DNA sequencing reaction using E827 and T827N3. The sequencing reaction was separated and analyzed on an <t>ABI</t> <t>PRISM</t> 377 DNA sequencer and ABI PRISM sequence analysis software.
    Abi Prism 377 Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism 377 dna sequencer/product/Thermo Fisher
    Average 94 stars, based on 446 article reviews
    Price from $9.99 to $1999.99
    abi prism 377 dna sequencer - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Primer fabrication using polymerase mediated oligonucleotide synthesis"

    Article Title: Primer fabrication using polymerase mediated oligonucleotide synthesis

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-10-344

    DNA cycle sequencing using an extendable primer . Panel A contains the design of an EOs and TOs for the sequencing of a region of the Escherichia coli streptomycin operon. Vertical bars indicate regions of clamp region of hybridisation between the EO and TO. Capital letters show actual sequence of the oligonucleotides and small, underlined letters indicate the extended region of the EO. N indicates a position with either A, T, G or C and S indicates a position with either G or C nucleotide. Panel B shows an electropherogram of a DNA sequencing reaction using E827 and T827N3. The sequencing reaction was separated and analyzed on an ABI PRISM 377 DNA sequencer and ABI PRISM sequence analysis software.
    Figure Legend Snippet: DNA cycle sequencing using an extendable primer . Panel A contains the design of an EOs and TOs for the sequencing of a region of the Escherichia coli streptomycin operon. Vertical bars indicate regions of clamp region of hybridisation between the EO and TO. Capital letters show actual sequence of the oligonucleotides and small, underlined letters indicate the extended region of the EO. N indicates a position with either A, T, G or C and S indicates a position with either G or C nucleotide. Panel B shows an electropherogram of a DNA sequencing reaction using E827 and T827N3. The sequencing reaction was separated and analyzed on an ABI PRISM 377 DNA sequencer and ABI PRISM sequence analysis software.

    Techniques Used: Sequencing, Hybridization, DNA Sequencing, Software

    DNA sequencing reactions using PMOS primers . Panels A and B contain an electropherograms of a DNA sequencing reactions using library derived E154/T422 and E167/T14 respectively. The sequencing reaction was separated and analyzed on an ABI PRISM 377 DNA sequencer and ABI PRISM sequence analysis software.
    Figure Legend Snippet: DNA sequencing reactions using PMOS primers . Panels A and B contain an electropherograms of a DNA sequencing reactions using library derived E154/T422 and E167/T14 respectively. The sequencing reaction was separated and analyzed on an ABI PRISM 377 DNA sequencer and ABI PRISM sequence analysis software.

    Techniques Used: DNA Sequencing, Derivative Assay, Sequencing, Software

    Related Articles

    Sequencing:

    Article Title: Use of GC Clamps in DHPLC Mutation Scanning
    Article Snippet: .. Sequencing reactions were performed using the Applied Biosystems Dye-Terminator Kit and analyzed on a ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA). .. First, an established denaturing gradient gel electrophoresis (DGGE) assay to DHPLC was adapted, since both DGGE and DHPLC-based mutation detection operate on the principle of detecting differences in melting behavior among individual species in a mixed population of DNA molecules.

    Article Title: Pigment Deficiency in Nightshade/Tobacco Cybrids Is Caused by the Failure to Edit the Plastid ATPase ?-Subunit mRNA W⃞
    Article Snippet: .. Sequencing products were analyzed using an ABI PRISM 377 DNA sequencer (Applied Biosystems, Foster City, CA). .. The following synthetic oligonucleotides (5′ to 3′) were used for vector construction and analysis of plastid transformants: HatpAfor, 5′-TAGCAAGCTTGGGTACAATAGGCATTGCTC-3′; HatpArev, 5′-TAGCAAGCTTAACCCTTTTCCTCAGGATCC-3′; WATfor, 5′-TTAATCATTTATGATGATTGGTCCAAACAAGCGCAAGCTT-3′; WATrev, 5′-CCAATCATCATAAATGATTAAAG-3′; CATfor, 5′-TTAATCATTTATGATGATCTCTCCAAACAAGCGCAAGCTT-3′; CATrev, 5′-GAGATCATCATAAATGATTAAAG-3′; Mu-edatpAfor, 5′-CTTATAATATACGACGACCCGTCCAAACAAGCGCAAGCTT-3′; Mu-edatpArev, 5′-CGGGTCGTCGTATATTATAAGAGTGTGTCGTTCACG-3′; EatpAfor, 5′-TTCCAGAATTCACATTACAATACCTTGCTCC-3′; EatpArev, 5′-TTCCAGAATTCTTTCCAAAAGGCGTGAATGC-3′; P1, 5′-ACGAAATTAGTAATATTATCC-3′; P2, 5′-ACTGCGGAGCCGTACAAATG-3′; P3, 5′-AAGCGGATGTAACTCAATCGG-3′; P4, 5′-CGGGTATAGTTTAGTGGTAA-3′; rpoC2for, 5′-GATTATTCAGACCTTAATCG-3′; rpoC2rev, 5′-AGGGCCAATTGAATTCCTAAT-3′; ndhAfor, 5′-GGCTGATATTATGACAATACTAGG-3′; ndhArev, 5′-GTAATACGACTCACTATAGGGGTAAAAACTCCATACAGTTG-3′.

    Article Title: Morphological differentiation despite gene flow in an endangered grasshopper
    Article Snippet: .. Cycle sequencing used Perkin Elmer BigDye 3.1 chemistry following the manufacturer's protocols analysed on an ABI Prism 377 DNA sequencer (Applied Biosystems, Inc., Foster City, California). .. Sequences were checked using SEQUENCHER version 4.10.1 (Gene Codes) and aligned with existing data using SeAl version 2.0 and GENEIOUS PRO version 5.3.4 [ , ].

    Article Title: Primer fabrication using polymerase mediated oligonucleotide synthesis
    Article Snippet: .. The cleaned sequencing reaction were analyzed on an ABI PRISM 377 DNA sequencer using the ABI PRISM sequence analysis software (Applera Corp., Norwalk, CT, USA) according to the manufacturer's instructions. .. Abbreviations PMOS: polymerase mediated oligonucleotide synthesis; TO: template oligonucleotide; EO: extendable oligonucleotide; PCR: polymerase chain reaction.

    Article Title: Expression, Gene Cloning, and Characterization of Five Novel Phytases from Four Basidiomycete Fungi: Peniophora lycii, Agrocybe pediades, a Ceriporia sp., and Trametes pubescens
    Article Snippet: .. The cDNA sequences from both strands were determined with the Dye Terminator Cycle Sequencing kit (Perkin-Elmer) and an Applied Biosystems ABI PRISM 377 DNA sequencer according to the manufacturer's instructions. .. The phytase-encoding cDNA inserts were subcloned into Aspergillus expression vector pHD414 ( ) or, for the A. pediades insert, into the pHD423 expression vector (a pHD414 derivative with a Kpn I site in the polylinker).

    Article Title: Origin and Evolution of Processed Pseudogenes That Stabilize Functional Makorin1 mRNAs in Mice, Primates and Other Mammals
    Article Snippet: .. Sequencing reactions were performed with BigDye Terminator v1.1 cycle sequencing kits (Applied Biosystems, Foster City, CA) and analyzed on an ABI PRISM 377 DNA sequencer (Applied Biosystems). .. To minimize sequencing errors, PCR primary products or cloned DNAs were sequenced two to six times in both directions.

    Ethanol Precipitation:

    Article Title: Source Bioaerosol Concentration and rRNA Gene-Based Identification of Microorganisms Aerosolized at a Flood Irrigation Wastewater Reuse Site
    Article Snippet: .. The reaction mixture was purified by ethanol precipitation with 80 μl of ethanol-water-3 M sodium acetate (5:1:0.1, pH 5), and then separated and analyzed on an automated ABI Prism 377 DNA sequencer (Applied Biosystems). ..

    Purification:

    Article Title: Source Bioaerosol Concentration and rRNA Gene-Based Identification of Microorganisms Aerosolized at a Flood Irrigation Wastewater Reuse Site
    Article Snippet: .. The reaction mixture was purified by ethanol precipitation with 80 μl of ethanol-water-3 M sodium acetate (5:1:0.1, pH 5), and then separated and analyzed on an automated ABI Prism 377 DNA sequencer (Applied Biosystems). ..

    Amplification:

    Article Title: Activities of Fluoroquinolones against Streptococcus pneumoniae Type II Topoisomerases Purified as Recombinant Proteins
    Article Snippet: .. Amplified templates for the quinolone resistance-determining region (QRDR) were sequenced by using an ABI PRISM 377 DNA sequencer (PE Applied Biosystems, Foster City, Calif.) with Big Dye terminator chemistry. .. The oligonucleotides used to prime the DNA sequencing reactions for sequencing of the QRDRs are described in Table .

    Software:

    Article Title: Primer fabrication using polymerase mediated oligonucleotide synthesis
    Article Snippet: .. The cleaned sequencing reaction were analyzed on an ABI PRISM 377 DNA sequencer using the ABI PRISM sequence analysis software (Applera Corp., Norwalk, CT, USA) according to the manufacturer's instructions. .. Abbreviations PMOS: polymerase mediated oligonucleotide synthesis; TO: template oligonucleotide; EO: extendable oligonucleotide; PCR: polymerase chain reaction.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Thermo Fisher abi prism 377 automated dna sequencer
    Abi Prism 377 Automated Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism 377 automated dna sequencer/product/Thermo Fisher
    Average 91 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    abi prism 377 automated dna sequencer - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    94
    Thermo Fisher abi prism 377 dna sequencer
    Abi Prism 377 Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism 377 dna sequencer/product/Thermo Fisher
    Average 94 stars, based on 446 article reviews
    Price from $9.99 to $1999.99
    abi prism 377 dna sequencer - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results