abi 9700 dual 384 well geneamp polymerase chain reaction pcr system  (Thermo Fisher)


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  • 99
    Name:
    Shandon Grosslab Senior Pathology Workstations
    Description:
    Multiple options to suit your specific needs Thermo Scientific Shandon Grosslab Senior Pathology Workstations provide superior quality and a variety of features
    Catalog Number:
    970022
    Price:
    None
    Applications:
    Anatomical Pathology|Clinical
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher abi 9700 dual 384 well geneamp polymerase chain reaction pcr system
    Multiple options to suit your specific needs Thermo Scientific Shandon Grosslab Senior Pathology Workstations provide superior quality and a variety of features
    https://www.bioz.com/result/abi 9700 dual 384 well geneamp polymerase chain reaction pcr system/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abi 9700 dual 384 well geneamp polymerase chain reaction pcr system - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Development of a fast PCR protocol enabling rapid generation of AmpFlSTR® Identifiler® profiles for genotyping of human DNA
    Article Snippet: .. The estimated duration time for this protocol is 3.5 hours when using a GeneAmp® PCR System 9700 thermal cycler (Applied Biosystems, Foster City, CA, USA) at the 9600 emulation mode (recommended by the manufacturer). .. The strategy employed in order to reduce time allotted to PCR amplification included the replacement of AmpliTaq Gold® with SpeedSTAR™ DNA polymerase, the use of a thermal cycler with greater efficiency (Bio-Rad C1000™, maximum ramp rate of 5°C/s) [ ] and modification of the cycling parameters including the adoption of a two-step PCR approach (where the annealing and extension steps are combined) and decreased hold times at all steps.

    Article Title: Highly sensitive amperometric detection of genomic DNA in animal tissues
    Article Snippet: .. First strand cDNA synthesis was carried out by incubating the mixture on a DNA thermal cycler (Gene Amp PCR System 9700, Applied Biosystems, Foster City) at 56°C for 50 min. .. The cDNA was subsequently used as template for the PCR amplification and biotinylation.

    Article Title: Molecular characterisation of Mycoplasma species isolated from the genital tract of Dorper sheep in South Africa
    Article Snippet: .. The mixtures were subjected to 10 minutes of initial denaturation at 94 ºC, followed by 35 cycles of amplification involving denaturation at 94 ºC for 30 seconds, primer annealing at 59 ºC for 45 seconds, primer extension at 72 ºC for 45 seconds, and a final primer extension at 72 ºC for 7 minutes, using a DNA thermal cycler (GeneAmp PCR system 9700, Applied Biosystems, SA). .. Amplified products were analysed together with a DNA ladder (O'Gene rulerTM , Fermentas Life Sciences, Inqaba Biotechnical, Industries [Pty] Ltd, Pretoria, SA) on a 1.5% agarose gel (Celtic Molecular Diagnostics, SA).

    Article Title: Development of Assays Using Hexokinase and Phosphoglucomutase Gene Sequences That Distinguish Strains of Leishmania tropica from Different Zymodemes and Microsatellite Clusters and Their Application to Palestinian Foci of Cutaneous Leishmaniasis
    Article Snippet: .. PCR amplification conditions for HK and PGM sequences HK and PGM PCR reactions were done in a volume of 25 µl that contained 1 µl of DNA template and PCR-Ready Supreme mix (Syntezza Bioscience, Jerusalem, Israel) in a Gene Amp PCR-system 9700 thermal cycler (Applied Biosystems, CA, USA), using the following conditions: initial denaturation for 5 min. at 98°C, followed by 38 cycles at 94°C for 45 s, 55°C for 45 s, and 72°C for 30 s and final elongation at 72°C for 7 min. For each reaction, DNA from strains of L. major and L. infantum , and strains of L. tropica belonging to the various zymodemes mentioned above were used as positive controls and distilled water was used as a negative control. .. Ten µl of each PCR product were run in 2.5% agarose gels.

    Article Title: Detection of macrolide and disinfectant resistance genes in clinical Staphylococcus aureus and coagulase-negative staphylococci
    Article Snippet: .. A final elongation at 72°C for 10 min was achieved in a DNA thermal cycler (GenAmp PCR system 9700-Applied Biosystem Int., USA). ..

    Article Title: Lacrimal Proline Rich 4 (LPRR4) Protein in the Tear Fluid Is a Potential Biomarker of Dry Eye Syndrome
    Article Snippet: .. RNA was extracted from the tissues using TRI reagent method, cDNA conversion was done from RNA using iScript™ cDNA synthesis kit (Bio-Rad, Herclus,CA) and Reverse transcriptase PCR (GeneAmp PCR system 9700 from Applied Biosystems) was done for LPRR4. ..

    Article Title: Novel Fluorescent Ligase Detection Reaction and Flow Cytometric Analysis of SYT-SSX Fusions in Synovial Sarcoma
    Article Snippet: .. The ligase detection reactions (LDR) were carried out in a PCR machine (Gene-Amp 9700; Applied Biosystems Inc.) as follows: 95°C for 5 minutes, 20 cycles of 95°C for 30 seconds, 65°C for 2 minutes, then 95°C for 5 minutes and cooling to 4°C. .. Following LDR, a 10-μl aliquot of ligation product was mixed with 10 μl of 6.0–8.0 μm streptavidin-coated polystyrene microbeads (Spherotech, Inc., Libertyville, IL) in a microfuge tube and incubated at room temperature for 30 minutes, with brief agitation of the tube at 15 minutes.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types
    Article Snippet: .. RT-PCR was performed with a 96-well GeneAmp Thermal Cycler 9700 (Applied Biosystems, USA) at 50℃ 30 min for cDNA synthesis. .. Quantitative PCR (q-PCR) q-PCR assays were performed in triplicate using an ABI PRISM 7300 SDS (Applied Biosystems, USA) with a 96-well plate format.

    Negative Control:

    Article Title: Development of Assays Using Hexokinase and Phosphoglucomutase Gene Sequences That Distinguish Strains of Leishmania tropica from Different Zymodemes and Microsatellite Clusters and Their Application to Palestinian Foci of Cutaneous Leishmaniasis
    Article Snippet: .. PCR amplification conditions for HK and PGM sequences HK and PGM PCR reactions were done in a volume of 25 µl that contained 1 µl of DNA template and PCR-Ready Supreme mix (Syntezza Bioscience, Jerusalem, Israel) in a Gene Amp PCR-system 9700 thermal cycler (Applied Biosystems, CA, USA), using the following conditions: initial denaturation for 5 min. at 98°C, followed by 38 cycles at 94°C for 45 s, 55°C for 45 s, and 72°C for 30 s and final elongation at 72°C for 7 min. For each reaction, DNA from strains of L. major and L. infantum , and strains of L. tropica belonging to the various zymodemes mentioned above were used as positive controls and distilled water was used as a negative control. .. Ten µl of each PCR product were run in 2.5% agarose gels.

    Amplification:

    Article Title: Molecular characterisation of Mycoplasma species isolated from the genital tract of Dorper sheep in South Africa
    Article Snippet: .. The mixtures were subjected to 10 minutes of initial denaturation at 94 ºC, followed by 35 cycles of amplification involving denaturation at 94 ºC for 30 seconds, primer annealing at 59 ºC for 45 seconds, primer extension at 72 ºC for 45 seconds, and a final primer extension at 72 ºC for 7 minutes, using a DNA thermal cycler (GeneAmp PCR system 9700, Applied Biosystems, SA). .. Amplified products were analysed together with a DNA ladder (O'Gene rulerTM , Fermentas Life Sciences, Inqaba Biotechnical, Industries [Pty] Ltd, Pretoria, SA) on a 1.5% agarose gel (Celtic Molecular Diagnostics, SA).

    Article Title: Development of Assays Using Hexokinase and Phosphoglucomutase Gene Sequences That Distinguish Strains of Leishmania tropica from Different Zymodemes and Microsatellite Clusters and Their Application to Palestinian Foci of Cutaneous Leishmaniasis
    Article Snippet: .. PCR amplification conditions for HK and PGM sequences HK and PGM PCR reactions were done in a volume of 25 µl that contained 1 µl of DNA template and PCR-Ready Supreme mix (Syntezza Bioscience, Jerusalem, Israel) in a Gene Amp PCR-system 9700 thermal cycler (Applied Biosystems, CA, USA), using the following conditions: initial denaturation for 5 min. at 98°C, followed by 38 cycles at 94°C for 45 s, 55°C for 45 s, and 72°C for 30 s and final elongation at 72°C for 7 min. For each reaction, DNA from strains of L. major and L. infantum , and strains of L. tropica belonging to the various zymodemes mentioned above were used as positive controls and distilled water was used as a negative control. .. Ten µl of each PCR product were run in 2.5% agarose gels.