Journal: Discover. Oncology

Article Title: Increased PAFAH1B3 was associated with poor prognosis and T-cell exhaustion microenvironment in hepatocellular carcinoma

doi: 10.1007/s12672-023-00845-6

Figure Lengend Snippet: Patients with increased PAFAH1B3 had a higher proportion of TEX infiltration. A , B Samples with high PAFAH1B3 expression showed considerably more CD8 + T cell and TEX infiltration than samples with low PAFAH1B3 expression. C Although there was no discernible difference in LAG3 expression levels between the two groups in the ICGC cohort, the expression of these genes was higher in the samples with high PAFAH1B3 than in those with low PAFAH1B3. D Patients with high PAFAH1B3 expression had a larger percentage of CD8 + T cells infiltrated than those with low PAFAH1B3 expression clinical samples. E Expression of all TEX marker genes was considerably higher in these patients. F The expression of PAFAH1B3 was significantly and positively correlated with the expression of TEX-related marker genes. G Results of immunofluorescence staining. ns, not significant. *p < 0.05; **p < 0.01; ***p < 0.001

Article Snippet: IHC tests using anti-PAFAH1B3 (Proteintech, China) or anti-CD8 (Abcam, UK) antibodies were separately performed on paraffin-embedded tissues from HCC patients.

Techniques: Expressing, Marker, Immunofluorescence, Staining

Journal: Hepatology (Baltimore, Md.)

Article Title: Intratumoral microbial heterogeneity affected tumor immune microenvironment and determined clinical outcome of HBV-related HCC

doi: 10.1097/HEP.0000000000000427

Figure Lengend Snippet: Discrepant gene signatures between two HCC molecular subtypes were mainly enriched in immune and metabolism. (A) Two HCC molecular subtypes were with different gene signatures by hierarchical cluster analysis. (B) Top 10 enriched GO terms in BPs, MFs, and CC. (C) Proportions of macrophages, CAFs, CD4 + T cells, CD8 + T cells, B cells, NK cells, and endothelial cells by EPIC. (D) Proportions of 22 immune cell subtypes by CIBERSORT. (E) IHC staining of CD68 + and CD163 + macrophages of two HCC molecular subtypes. Abbreviations: BP, biological process; CAF, cancer-associated fibroblast; CC, cellular component; GO, gene ontology; IHC, immunohistochemistry, MF, molecular function.

Article Snippet: Sections were incubated with monoclonal antibodies against CD3 (ab16669, Abcam), CD8 (ab93278, Abcam), CD68 (ZM-0060, ZSBio), CD163 (ZM-0428, ZSBio), and PD-L1 (ab210931, Abcam) and then stained with avidin-biotin-peroxidase complex method, as described.

Techniques: Immunohistochemistry