Journal: Heliyon
Article Title: Itraconazole inhibits proliferation, induces apoptosis, and reduces angiogenesis of hemangioma endothelial cells by downregulating the hedgehog signaling pathway
doi: 10.1016/j.heliyon.2023.e19244
Figure Lengend Snippet: Identification of HemECs. The expression of CD31, CD34, factor VIII, and GLUT1 in HemECs was studied using immunofluorescence staining. Positive expression was observed for CD31, CD34, factor VIII, and GLUT1 (A, B, C and D) . The cytoplasm and nucleus were stained with FITC (green) and DAPI (blue), respectively. Scale bar = 0.05 mm.
Article Snippet: The cells were treated with primary antibodies overnight at 4 °C: CD31 (1:50; Proteintech; 11265-1-AP), CD34 (1:50; Proteintech; 14486-1-AP), factor VIII (Abcam; 1:500; ab275376), and GLUT1 (1:50; Proteintech; 21829-1-AP).
Techniques: Expressing, Immunofluorescence, Staining