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antibodies against factor viii  (Abcam)


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    Structured Review

    Abcam antibodies against factor viii
    Antibodies Against Factor Viii, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab78852/Anti-Factor+VIII+antibody/pmc09992991-66-8-15
    Average 99 stars, based on 74 article reviews
    antibodies against factor viii - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Affinity Purification:

    Article Title: Novel microfluidic platform for automated lab-on-chip testing of hypercoagulability panel
    Article Snippet: .. Activated beads were coated with 60 μg/100 μl of affinity purified mouse monoclonal antibodies to ATIII, protein S, FVIII (Abcam), protein C (Affinity Biologicals Inc.) in 50-mmol/l MES (pH 5) solution. .. For anticardiolipin detection, activated beads were coated with 25 μg/100 μl of human β2GP1 protein (Aniara) with 35 μg/100 μl cardiolipin (Sigma-Aldrich) in 50-mmol/l MES (pH 5) solution.

    Immunohistochemical staining:

    Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation
    Article Snippet: .. Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam). ..

    Staining:

    Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation
    Article Snippet: .. Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam). ..

    Coagulation:

    Article Title: Novel microfluidic platform for automated lab-on-chip testing of hypercoagulability panel
    Article Snippet: .. Dynal beads were coated with coagulation factor antibodies (60 μg/100 μl); anti-ATIII, antiprotein S, anti-FVIII (Abcam), antiprotein C (Affinity Biologicals Inc.) and human β2GP1 protein (Aniara) with cardiolipin (Sigma-Aldrich) as per manufacturer’s instructions (Dynal; Invitrogen Co., Carlsbad, California, USA). .. Briefly, Dynal M-270 carboxylic acid beads were washed twice with 0.01-mol/l NaOH for 10 min each followed by washes (three times) in deionized water.

    Bioprocessing:

    Article Title: Novel microfluidic platform for automated lab-on-chip testing of hypercoagulability panel
    Article Snippet: .. Activated beads were coated with 60 μg/100 μl of affinity purified mouse monoclonal antibodies to ATIII, protein S, FVIII (Abcam), protein C (Affinity Biologicals Inc.) in 50-mmol/l MES (pH 5) solution. .. For anticardiolipin detection, activated beads were coated with 25 μg/100 μl of human β2GP1 protein (Aniara) with 35 μg/100 μl cardiolipin (Sigma-Aldrich) in 50-mmol/l MES (pH 5) solution.

    Labeling:

    Article Title: Correction of a Factor VIII genomic inversion with designer-recombinases
    Article Snippet: iPSCs were cultured in 6-well plates (Corning) and were differentiated into endothelial cells as described earlier. .. At day 3 cells were fixed in 4% paraformaldehyde (PFA) at room temperature for 20 min, permeabilized and immunostained following standard protocol using anti-Factor VIII antibody (Abcam, ab236284, 1:200) and fluorescently labeled secondary antibody (Invitrogen, Alexa fluor 546 goat anti-rabbit, 1:500). .. Additionally, nuclei were labeled with Hoechst 33342 (Invitrogen, H3570, 1:5000).

    Incubation:

    Article Title: Hsa-miR-5581-3p and Hsa-miR-542-3p Target the F8 Gene in Hemophilia A without F8 Mutations
    Article Snippet: .. Primary antibodies against GAPDH (ab8245, 1:5000, Abcam, Cambridge, MA, USA) and FVIII (ab171825, 1:1000, Abcam) were incubated with the membrane on a shaker for 1 h at room temperature, followed by incubation of secondary antibody for 1 h at room temperature. ..

    Membrane:

    Article Title: Hsa-miR-5581-3p and Hsa-miR-542-3p Target the F8 Gene in Hemophilia A without F8 Mutations
    Article Snippet: .. Primary antibodies against GAPDH (ab8245, 1:5000, Abcam, Cambridge, MA, USA) and FVIII (ab171825, 1:1000, Abcam) were incubated with the membrane on a shaker for 1 h at room temperature, followed by incubation of secondary antibody for 1 h at room temperature. ..



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    Representative images of histology. (A) Hematoxylin and eosin(H&E) staining(40X). (B) Immunohistochemical staining of <t>factor</t> <t>VIII(40X).</t> (C) Alcian-blue staining(40X). (D) Picrosirius-red staining(10X). Scale bars depict 100µm.
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    Image Search Results


    Identification of HemECs. The expression of CD31, CD34, factor VIII, and GLUT1 in HemECs was studied using immunofluorescence staining. Positive expression was observed for CD31, CD34, factor VIII, and GLUT1 (A, B, C and D) . The cytoplasm and nucleus were stained with FITC (green) and DAPI (blue), respectively. Scale bar = 0.05 mm.

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    Figure Lengend Snippet: Identification of HemECs. The expression of CD31, CD34, factor VIII, and GLUT1 in HemECs was studied using immunofluorescence staining. Positive expression was observed for CD31, CD34, factor VIII, and GLUT1 (A, B, C and D) . The cytoplasm and nucleus were stained with FITC (green) and DAPI (blue), respectively. Scale bar = 0.05 mm.

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    Techniques: Expressing, Immunofluorescence, Staining

    Representative images of histology. (A) Hematoxylin and eosin(H&E) staining(40X). (B) Immunohistochemical staining of factor VIII(40X). (C) Alcian-blue staining(40X). (D) Picrosirius-red staining(10X). Scale bars depict 100µm.

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    Figure Lengend Snippet: Representative images of histology. (A) Hematoxylin and eosin(H&E) staining(40X). (B) Immunohistochemical staining of factor VIII(40X). (C) Alcian-blue staining(40X). (D) Picrosirius-red staining(10X). Scale bars depict 100µm.

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