goat anti pig igg h l fitc (Danaher Inc)

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Danaher Inc goat anti pig igg h l fitc
Goat Anti Pig Igg H L Fitc, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab6916/Goat+Anti-Pig+IgG+H%26L/10__1155_slash_2023_slash_2830485-43-0-9
Average 99 stars, based on 39 article reviews

goat anti pig igg h l fitc - by Bioz Stars, 2026-10

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Article Title: Attenuation of a Highly Pathogenic Porcine Deltacoronavirus Strain CZ2020 by a Serial Passage In Vitro
Article Snippet: Goat anti-pig IgG H&L (FITC) (ab6911) was purchased from Abcam.

Article Title: A Triple Gene-Deleted Pseudorabies Virus-Vectored Subunit PCV2b and CSFV Vaccine Protects Pigs against PCV2b Challenge and Induces Serum Neutralizing Antibody Response against CSFV
Article Snippet: Secondary antibodies, donkey anti-mouse IgG Alexa fluor 488 (#A-21202, Thermo Fisher Scientific ® ), goat anti-mouse IgG horseradish peroxidase (HRP) (#32430, Thermo Fisher Scientific ® ), goat anti-pig IgG HRP (#ab102135, Abcam, Waltham, MA, USA), rabbit anti-mouse HRP (Agilent Technologies, Waldbronn, Germany), and FITC-rabbit anti-porcine IgG-H+L [17-9111, Invitrogen] were purchased.

Incubation:

Article Title: Delivery of antigen to porcine dendritic cells by fusing antigen with porcine dendritic cells targeting peptide
Article Snippet: .. After washing thrice with PBST (PBS containing 1% Tween-20), the plates were blocked with 5% skim milk at 37°C for 2 h. After washing thrice with PBST, the collected samples, prepared in triplicate, were diluted with PBS and incubated at 37°C for 1 h. Then, a conjugated goat anti-pig IgG or IgA antibody (Abcam, Cambridge, UK) was added to each well (1:5000) and incubated for 1 h at 37°C. .. The color was developed using tetramethylbenzidine (TMB; Sigma, Ronkonkoma, NY, USA) as a substrate, and absorbance was measured at 490 nm.

Article Title: Antigenic and immunogenic properties of recombinant proteins consisting of two immunodominant African swine fever virus proteins fused with bacterial lipoprotein OprI
Article Snippet: The membranes were blocked with 5% skim milk for 2 h at room temperature and then incubated with anti-histidine monoclonal antibody (1:5000 dilution, Abcam, Cambridge, UK) or anti-ASFV swine serum (1:300 dilution, Diagnostic Products Center, LVRI, China) overnight at 4 °C. .. After washing five times with PBS containing 0.05% Tween-20 (PBST), the membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (1:5000 dilution, Abcam) or goat anti-pig antibody (1:5000 dilution, Abcam) for 1 h at room temperature. .. After being washed with PBST, the membranes were developed using enhanced chemiluminescence reagent (Thermo Fisher Scientific).

Article Title: Immune Responses in Pregnant Sows Induced by Recombinant Lactobacillus johnsonii Expressing the COE Protein of Porcine Epidemic Diarrhea Virus Provide Protection for Piglets against PEDV Infection
Article Snippet: .. After washing, the plate was blocked with 5% skim milk at 37 °C for 2 h. After washing, the wells were incubated with sera samples (diluted at 1:10), colostrum samples (diluted at 1:10), and nose and rectal swab samples at 37 °C for 1 h. After washing, the wells were incubated with goat anti-pig IgG or IgA antibody (HRP-conjugated, diluted at 1:5000) (Abcam, Cambridge, Cambridgeshire, UK) at 37 °C for 1 h followed by washing. .. Absorbance was measured at OD450 nm by color change of o-phenylenediamine dihydrochloride (Sigma, Ronkonkoma, NY, USA).

Article Title: Crude F4 fimbriae extracts: its feasibility to be a candidate for Escherichia coli vaccine development for the prevention of diarrhea disease caused by E. coli in piglets
Article Snippet: .. After washing three times, goat anti-pig IgG HRP antibody (Abcam, Cambridge, England) for detecting IgG antibody or goat anti-pig IgA HRP antibody (Abcam, Cambridge, England) for detecting IgA antibody diluted at 1:20,000 in PBST was added into the wells (50 μl /well), followed by an incubation 37oC in moisture chamber for 1 hour. .. After washing, TMB substrate (Sigma-Aldrich, Darmstadt, Germany) was added (50 μl /well), and the plates were incubated in the dark at room temperature for 10-15 minutes.

Article Title: Immunodominant and Neutralizing Linear B-Cell Epitopes Spanning the Spike and Membrane Proteins of Porcine Epidemic Diarrhea Virus
Article Snippet: .. After a 2-h incubation at RT, the plates were washed three times with PBST, and goat anti-pig IgG HRP antibody (Abcam) diluted 1:10,000 in PBST containing 1% FBS was added to the well (60 μl/well), followed by an incubation at RT for 90 min. After a three-time wash with PBST, the TMB substrate (BioLegend) was added (70 μl/well) and the plate was incubated in the dark at RT for 30 min, allowing the color to develop. .. The reaction was stopped by adding 35 μl of 1 M sulfuric acid (H 2 SO 4 ), and the optical density was measured at a wavelength of 450 (OD450) (Multiskan FC, Thermo Scientific).

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