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human syndecan-1 elisa kit (cd138) 1x96 test  (Advisains)


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    Advisains human syndecan-1 elisa kit (cd138) 1x96 test
    Human Syndecan 1 Elisa Kit (Cd138) 1x96 Test, supplied by Advisains, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab46506/custom%40ab46506%4042237724?v=Advisains
    Average 99 stars, based on 62 article reviews
    human syndecan-1 elisa kit (cd138) 1x96 test - by Bioz Stars, 2026-07
    99/100 stars

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    human syndecan-1 elisa kit (cd138) 1x96 test  (Advisains)
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    anti heparanase 1  (Abcam)
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    HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and <t>heparanase-1</t> protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for hyaluronidase activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.
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    HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and heparanase-1 protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for hyaluronidase activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.

    Journal: Renal Failure

    Article Title: HYAL1 deficiency attenuates lipopolysaccharide-triggered renal injury and endothelial glycocalyx breakdown in septic AKI in mice

    doi: 10.1080/0886022X.2023.2188966

    Figure Lengend Snippet: HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and heparanase-1 protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for hyaluronidase activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.

    Article Snippet: Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, proteins were transferred onto polyvinylidene difluoride membranes, and then incubated with primary antibodies including anti-heparanase-1 (ab288438; 1:1000), anti-syndecan-1 (ab128936; 1:1000), anti-p-AMPK (ab133448; 1:1000), anti-AMPK (ab32047; 1:1000), anti-p-mTOR (ab109268; 1:1000), anti-mTOR (ab134903, 1:10000), anti-LC3-I (ab62721; 1:1000), anti-LC3-II (ab192890; 1:2000), anti-p62 (ab109012; 1:10000) and GAPDH (ab8245; 1:500) at 4 °C overnight and subsequently with horseradish peroxidase-labeled IgG (ab6721; 1:1000) secondary antibody at room temperature for 2 h. All antibodies were purchased from Abcam.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Activity Assay, Immunofluorescence, Staining, Fluorescence, Immunohistochemistry