recombinant anti-fgl1 antibody  (Advisains)

Journal: Chinese Medicine

Article Title: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer

doi: 10.1186/s13020-023-00820-x

Figure Lengend Snippet: PCR primer sequences

Article Snippet: Antibodies against METTL3 (ab195352, 1:1000), YTHDF2 (ab275037, 1:1000) and GPX4 (ab125066, 1:1000) were purchased from Abcam.

Techniques:

Journal: Chinese Medicine

Article Title: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer

doi: 10.1186/s13020-023-00820-x

Figure Lengend Snippet: Curdione promoted the expression of METTL14 and YTHDF2 and reduced the expression of SLC7A11, SLC3A2, HOXA13, and GPX4. Mice were administered with L-CUR (50 mg/kg), M-CUR (100 mg/kg), and H-CUR (200 mg/kg) for 22 days. a – j . The protein levels of METTL3, METTL4, METTL14, YTHDF2, SLC7A11, SLC3A2, HOXA13, and GPX4 in CRC xenograft mice were measured by Western blotting. Values are expressed as the mean ± SD of three independent experiments. (n = 3) * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control group

Article Snippet: Antibodies against METTL3 (ab195352, 1:1000), YTHDF2 (ab275037, 1:1000) and GPX4 (ab125066, 1:1000) were purchased from Abcam.

Techniques: Expressing, Western Blot, Control

Journal: Chinese Medicine

Article Title: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer

doi: 10.1186/s13020-023-00820-x

Figure Lengend Snippet: Curdione promoted the expression of METTL14, YTHDF2, and PTGS2 and reduced the expression of SLC7A11, SLC3A2, HOXA13, and GPX4. Mice were administered with L-CUR (50 mg/kg), M-CUR (100 mg/kg), and H-CUR (200 mg/kg) for 22 days. a – i . The mRNA levels of METTL3, METTL4, METTL14, SLC3A2, SLC7A11, HOXA13, YTHDF2, GPX4, and PTGS2 in CRC tissues were measured by qRT-PCR. Values are expressed as the mean ± SD of three independent experiments. (n = 3) * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control group

Article Snippet: Antibodies against METTL3 (ab195352, 1:1000), YTHDF2 (ab275037, 1:1000) and GPX4 (ab125066, 1:1000) were purchased from Abcam.

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Chinese Medicine

Article Title: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer

doi: 10.1186/s13020-023-00820-x

Figure Lengend Snippet: PCR primer sequences

Article Snippet: Antibodies against METTL3 (ab195352, 1:1000), YTHDF2 (ab275037, 1:1000) and GPX4 (ab125066, 1:1000) were purchased from Abcam.

Techniques:

Journal: Chinese Medicine

Article Title: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer

doi: 10.1186/s13020-023-00820-x

Figure Lengend Snippet: Curdione promoted the expression of METTL14 and YTHDF2 and reduced the expression of SLC7A11, SLC3A2, HOXA13, and GPX4. a – g . The protein levels of METTL14, YTHDF2, SLC7A11, SLC3A2, HOXA13, and GPX4 in CT26 cells were measured by Western blotting after 12.5 μM, 25 μM, and 50 μM curdione interventions for 48 h. Values are expressed as the mean ± SD of three independent experiments. (n = 3) * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control group

Article Snippet: Antibodies against METTL3 (ab195352, 1:1000), YTHDF2 (ab275037, 1:1000) and GPX4 (ab125066, 1:1000) were purchased from Abcam.

Techniques: Expressing, Western Blot

Journal: Chinese Medicine

Article Title: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer

doi: 10.1186/s13020-023-00820-x

Figure Lengend Snippet: Curdione promoted the expression of METTL14 and YTHDF2 and reduced the expression of SLC7A11, SLC3A2, HOXA13, and GPX4. Mice were administered with L-CUR (50 mg/kg), M-CUR (100 mg/kg), and H-CUR (200 mg/kg) for 22 days. a – j . The protein levels of METTL3, METTL4, METTL14, YTHDF2, SLC7A11, SLC3A2, HOXA13, and GPX4 in CRC xenograft mice were measured by Western blotting. Values are expressed as the mean ± SD of three independent experiments. (n = 3) * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control group

Article Snippet: Antibodies against METTL3 (ab195352, 1:1000), YTHDF2 (ab275037, 1:1000) and GPX4 (ab125066, 1:1000) were purchased from Abcam.

Techniques: Expressing, Western Blot

Journal: Chinese Medicine

Article Title: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer

doi: 10.1186/s13020-023-00820-x

Figure Lengend Snippet: Curdione promoted the expression of METTL14, YTHDF2, and PTGS2 and reduced the expression of SLC7A11, SLC3A2, HOXA13, and GPX4. Mice were administered with L-CUR (50 mg/kg), M-CUR (100 mg/kg), and H-CUR (200 mg/kg) for 22 days. a – i . The mRNA levels of METTL3, METTL4, METTL14, SLC3A2, SLC7A11, HOXA13, YTHDF2, GPX4, and PTGS2 in CRC tissues were measured by qRT-PCR. Values are expressed as the mean ± SD of three independent experiments. (n = 3) * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control group

Article Snippet: Antibodies against METTL3 (ab195352, 1:1000), YTHDF2 (ab275037, 1:1000) and GPX4 (ab125066, 1:1000) were purchased from Abcam.

Techniques: Expressing, Quantitative RT-PCR

Journal: Chinese Medicine

Article Title: Curdione induces ferroptosis mediated by m6A methylation via METTL14 and YTHDF2 in colorectal cancer

doi: 10.1186/s13020-023-00820-x

Figure Lengend Snippet: Curdione regulated ferroptosis in colorectal cancer via N6-methyladenosine. Curdione upregulated the methylation transferase METTL14, which increased m6A modification in SLC7A11 and SLC3A2 mRNAs. The m6A reader YTHDF2 then promoted the degradation of SLC7A11 and SLC3A2 mRNAs in a m6A-dependent manner, reducing their stability, which triggered reactive oxygen species accumulation and ultimately led to CRC ferroptosis

Article Snippet: Antibodies against METTL3 (ab195352, 1:1000), YTHDF2 (ab275037, 1:1000) and GPX4 (ab125066, 1:1000) were purchased from Abcam.

Techniques: Methylation, Modification

Journal: Poultry Science

Article Title: Extracellular vesicle-coupled miRNA profiles of chicken seminal plasma and their potential interaction with recipient cells

doi: 10.1016/j.psj.2023.103099

Figure Lengend Snippet: The characteristic features of chicken seminal plasma extracellular vesicles (SPEVs). (A) Transmission electron microscope images of SPEVs isolated from 4 chicken breeds, Beijing-You chicken (BJY), Dwarf chicken (DY), Recessive white chicken (IV), and White Leghorn (WL). Bar = 200 µm. SPEVs possess typical cup-shaped vesicle structures. (B) Nanoparticle tracking analysis of particle size distribution profiles of SPEVs from 4 chicken breeds. Most particle sizes ranged from 50 to 200 nm which is consistent with the characteristics of EVs. (C) Western blots analysis of ALIX, TSG101, and HSP70 proteins of SPEVs from 4 chicken breeds.

Article Snippet: The 3 primary antibodies used were ALIX (A2215, 1:500, Abclonal, Wuhan, China), HSP70 (A20819, 1:2,500, Abclonal), and TSG101 (ab275018-1kit, 1:1,000, Abcam, Shanghai, China).

Techniques: Transmission Assay, Microscopy, Isolation, Western Blot

Journal: Cellular & Molecular Biology Letters

Article Title: MiR-15b-5p and PCSK9 inhibition reduces lipopolysaccharide-induced endothelial dysfunction by targeting SIRT4

doi: 10.1186/s11658-023-00482-5

Figure Lengend Snippet: i-miR-15b opposed the LPS-related inflammation. A Hsa-miR-15b-5p expression measured by qRT–PCR in TeloHAEC transfected with antagomir negative control (NC) or antagomiR hsa-miR-15b-5p (i-miR-15b). Detection of B LDH, C NO, D VCAM1, E ICAM1, F MCP-1, G IL-1β, and H IL-18 levels. Assessment of caspase-4 I activity and J levels. SIRT4 levels assessed by K ELISA assay and L immunoblotting. M Representative images and N FACS analysis of pyroptosis. O Intracellular NLRP3 levels assessed by FACS detection. Mean ± SD, n = 3. M, molecular weight markers; lane 1, Ctr; lane 2, NC; lane 3, NC + LPS; lane 4, i-miR-15b; lane 5, i-miR-15b + LPS. Scale bars = 100 μm. * p < 0.05 versus NC, ‡ p < 0.01 versus NC, § p < 0.001 versus NC, • p < 0.05 versus NC + LPS, † p < 0.01 versus NC + LPS. Statistical analysis of data was performed using one-way ANOVAs.

Article Snippet: Inflammatory mediators (VCAM1, ab223591; ICAM1, ab174445; MCP-1, ab179886; IL-1β, ab214025; IL-18, ab215539, all from Abcam, Cambridge, UK), caspase-4 (ab275098, Abcam, Cambridge, UK), PCSK9 (DPC900, R&D Systems, Minneapolis, MN, USA, Inc.), SIRT4 (MBS2705670, MyBioSource, San Diego, CA, USA), and LOX-1 (EHOLR1, Invitrogen, Waltham, MA, USA) levels were determined by human enzyme-linked immunosorbent assays (ELISA), following the specific instructions.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Negative Control, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Molecular Weight