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exosome isolation and analysis kit - flow cytometry, plasma (cd63 / cd81) 25 tests (Advisains)

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Advisains exosome isolation and analysis kit - flow cytometry, plasma (cd63 / cd81) 25 tests
Exosome Isolation And Analysis Kit Flow Cytometry, Plasma (Cd63 / Cd81) 25 Tests, supplied by Advisains, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab267479/custom%40ab267479%4040921191?v=Advisains
Average 99 stars, based on 59 article reviews

exosome isolation and analysis kit - flow cytometry, plasma (cd63 / cd81) 25 tests - by Bioz Stars, 2026-07

99/100 stars

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exosome isolation and analysis kit - flow cytometry, plasma (cd63 / cd81) 25 tests (Advisains)

Advisains exosome isolation and analysis kit - flow cytometry, plasma (cd63 / cd81) 25 tests
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Journal: Journal of Nanobiotechnology

Article Title: Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification

doi: 10.1186/s12951-023-02000-3

Figure Lengend Snippet: Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, CD9, and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm

Article Snippet: After blocking with 5% non-fat milk for 1 h, the membrane was incubated overnight at 4 °C with primary antibody, including CD9 (ab92726, 1:1000, abcam), CD81 (ab109201, 1:1000, abcam), TSG101 (bs-1365R, 1:1000, bioss), Runx2 (ab23981, 1:2000, abcam), BMP2 (bs-10696R, 1:1000, bioss), GAPDH (10494-1-AP, 1:4000, proteintech), BMP4 (bs-1374R, 1:1000, bioss), Crim1 (bs-21654R, 1:1000, bioss), followed by incubation with the horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature.

Techniques: Derivative Assay, Transmission Assay, Western Blot, Incubation, Staining