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Structured Review

Abcam anti cd9
Anti Cd9, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 757 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab267479/anti-NCAM1+antibody/10__4314_slash_tjpr__v22i3__8-61-5-24
Average 99 stars, based on 757 article reviews
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Images

Related Articles

Western Blot:

Article Title: Intratracheal Transplantation of Amnion-Derived Mesenchymal Stem Cells Ameliorates Hyperoxia-Induced Neonatal Hyperoxic Lung Injury via Aminoacyl-Peptide Hydrolase
Article Snippet: The morphology of the exosomes was observed using transmission electron microscopy (TEM; JEOL-1200-EX II; JEOL, Tokyo, Japan). .. Exosomes were identified using specific antibodies targeting CD63 (ab217345, Abcam), CD9 (ab92726, Abcam), CD81 (ab109201, Abcam), and heat shock protein 70 (Hsp70; ab79852, Abcam), which were used for western blotting analysis. ..

Article Title: Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia
Article Snippet: EVs or cells were lysed with radioimmunoprecipitation lysis buffer (Invitrogen). .. In brief, immunoblotting was performed by incubating with corresponding primary antibodies against Alix (ab275377, Abcam, Cambridge, MA), CD63 (ab59479, Abcam), CD9 (ab92726, Abcam), CD81 (ab79559, Abcam), nucleus related antigen 67 (Ki67) (ab16667, Abcam), proliferating cell nuclear antigen (PCNA) (ab29, Abcam), TFPI2 (ab186747, Abcam), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (ab8245, Abcam). ..

Article Title: LPS-induced macrophage HMGB1-loaded extracellular vesicles trigger hepatocyte pyroptosis by activating the NLRP3 inflammasome
Article Snippet: .. Raw264.7-EVs were identified by transmission electron microscopy and NTA (NS3000, Worcestershire, UK), tetraspan molecules CD63 (ab10895, Abcam, Cambridge, UK), CD9 (ab92726, Abcam, Cambridge, UK), CD81 (18250-1-AP, Proteintech, Rosemont, USA) were verified by western blotting. ..

Incubation:

Article Title: WITHDRAWN: Exosomes from microRNA-145-5p-modified HUCB-MSCs attenuate CCl4-induced hepatic fibrosis via down-regulating FSCN1 expression.
Article Snippet: Objective: Dysregulated microRNA-145-5p (miR-145-5p) contributes to hepatic fibrosis (HF) while extracellular vesicles have the therapeutic capacities to treat this disease.. Based on that, this work is initiated from human umbilical cord blood mesenchymal stem cells (HUCB-MSCs)-derived exosomes delivering miR-145-5p to regulate fascin actin-bundling protein 1 (FSCN1) for attenuating HF.. Methods: A rat model of HF was established using carbon tetrachloride (CCl4) intraperitoneal injection.

Membrane:

Article Title: WITHDRAWN: Exosomes from microRNA-145-5p-modified HUCB-MSCs attenuate CCl4-induced hepatic fibrosis via down-regulating FSCN1 expression.
Article Snippet: Objective: Dysregulated microRNA-145-5p (miR-145-5p) contributes to hepatic fibrosis (HF) while extracellular vesicles have the therapeutic capacities to treat this disease.. Based on that, this work is initiated from human umbilical cord blood mesenchymal stem cells (HUCB-MSCs)-derived exosomes delivering miR-145-5p to regulate fascin actin-bundling protein 1 (FSCN1) for attenuating HF.. Methods: A rat model of HF was established using carbon tetrachloride (CCl4) intraperitoneal injection.

Imaging:

Article Title: WITHDRAWN: Exosomes from microRNA-145-5p-modified HUCB-MSCs attenuate CCl4-induced hepatic fibrosis via down-regulating FSCN1 expression.
Article Snippet: Objective: Dysregulated microRNA-145-5p (miR-145-5p) contributes to hepatic fibrosis (HF) while extracellular vesicles have the therapeutic capacities to treat this disease.. Based on that, this work is initiated from human umbilical cord blood mesenchymal stem cells (HUCB-MSCs)-derived exosomes delivering miR-145-5p to regulate fascin actin-bundling protein 1 (FSCN1) for attenuating HF.. Methods: A rat model of HF was established using carbon tetrachloride (CCl4) intraperitoneal injection.

other:

Article Title: Macrophagic Extracellular Vesicle CXCL2 Recruits and Activates the Neutrophil CXCR2/PKC/NOX4 Axis in Sepsis
Article Snippet: Primary Abs against Ly-6G (ab25377; Abcam, Cambridge, U.K.), CD63 (ab10895; Abcam), CD9 (ab92726; Abcam), CD81 (18250-1-AP; Proteintech), CXCL2 (26791-1-AP; Proteintech), CXCR2 (ab14935; Proteintech), PKC (ab19301; Abcam), and NOX4 (14347-1-AP; Proteintech) were obtained from the indicated sources.

Transmission Assay:

Article Title: LPS-induced macrophage HMGB1-loaded extracellular vesicles trigger hepatocyte pyroptosis by activating the NLRP3 inflammasome
Article Snippet: .. Raw264.7-EVs were identified by transmission electron microscopy and NTA (NS3000, Worcestershire, UK), tetraspan molecules CD63 (ab10895, Abcam, Cambridge, UK), CD9 (ab92726, Abcam, Cambridge, UK), CD81 (18250-1-AP, Proteintech, Rosemont, USA) were verified by western blotting. ..

Electron Microscopy:

Article Title: LPS-induced macrophage HMGB1-loaded extracellular vesicles trigger hepatocyte pyroptosis by activating the NLRP3 inflammasome
Article Snippet: .. Raw264.7-EVs were identified by transmission electron microscopy and NTA (NS3000, Worcestershire, UK), tetraspan molecules CD63 (ab10895, Abcam, Cambridge, UK), CD9 (ab92726, Abcam, Cambridge, UK), CD81 (18250-1-AP, Proteintech, Rosemont, USA) were verified by western blotting. ..



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Exosome Isolation And Analysis Kit Flow Cytometry, Plasma (Cd63 / Cd81) 25 Tests, supplied by Advisains, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, <t>CD9,</t> and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm
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Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, <t>CD9,</t> and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm
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Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, <t>CD9,</t> and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm
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Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, <t>CD9,</t> and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm
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Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, <t>CD9,</t> and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm
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Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, <t>CD9,</t> and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm
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Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, <t>CD9,</t> and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm
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Figure 1. Characteristics of SVF-EVs. (A) TEM images of SVF-EVs. (B) NTA analysis of SVF-EVs. The confirmed size distribution with a range of diameter around 100–300 nm. (C) Western blot shows that SVF- EVs express tetraspanin proteins like <t>TSG101</t> and CD81. (D) Western blot reveals that SVF-EVs encapsulate proteins such as PPARγ, C/EBPα, Ang-1, and VEGF. (E) Confocal images verify the internalization of PKH26- labeled SVF-EVs (red) into endothelial cell (CD31-labeled, green), and the amount of endocytosed SVF-EVs after co-culturing for 4 h is more than that for 2 h (F).
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Image Search Results


Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, CD9, and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm

Journal: Journal of Nanobiotechnology

Article Title: Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification

doi: 10.1186/s12951-023-02000-3

Figure Lengend Snippet: Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, CD9, and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm

Article Snippet: After blocking with 5% non-fat milk for 1 h, the membrane was incubated overnight at 4 °C with primary antibody, including CD9 (ab92726, 1:1000, abcam), CD81 (ab109201, 1:1000, abcam), TSG101 (bs-1365R, 1:1000, bioss), Runx2 (ab23981, 1:2000, abcam), BMP2 (bs-10696R, 1:1000, bioss), GAPDH (10494-1-AP, 1:4000, proteintech), BMP4 (bs-1374R, 1:1000, bioss), Crim1 (bs-21654R, 1:1000, bioss), followed by incubation with the horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature.

Techniques: Derivative Assay, Transmission Assay, Western Blot, Incubation, Staining

Figure 1. Characteristics of SVF-EVs. (A) TEM images of SVF-EVs. (B) NTA analysis of SVF-EVs. The confirmed size distribution with a range of diameter around 100–300 nm. (C) Western blot shows that SVF- EVs express tetraspanin proteins like TSG101 and CD81. (D) Western blot reveals that SVF-EVs encapsulate proteins such as PPARγ, C/EBPα, Ang-1, and VEGF. (E) Confocal images verify the internalization of PKH26- labeled SVF-EVs (red) into endothelial cell (CD31-labeled, green), and the amount of endocytosed SVF-EVs after co-culturing for 4 h is more than that for 2 h (F).

Journal: Scientific reports

Article Title: Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors.

doi: 10.1038/s41598-022-27176-w

Figure Lengend Snippet: Figure 1. Characteristics of SVF-EVs. (A) TEM images of SVF-EVs. (B) NTA analysis of SVF-EVs. The confirmed size distribution with a range of diameter around 100–300 nm. (C) Western blot shows that SVF- EVs express tetraspanin proteins like TSG101 and CD81. (D) Western blot reveals that SVF-EVs encapsulate proteins such as PPARγ, C/EBPα, Ang-1, and VEGF. (E) Confocal images verify the internalization of PKH26- labeled SVF-EVs (red) into endothelial cell (CD31-labeled, green), and the amount of endocytosed SVF-EVs after co-culturing for 4 h is more than that for 2 h (F).

Article Snippet: Then membranes were blocked with 5% skimmed milk, and incubated with specific antibodies against TSG101 (ab133586, Abcam, UK), CD81(ab109201, Abcam, UK), PPARγ (sc-7273, Santa, USA), CEBPα (sc-365318, Santa, USA), Ang-1 (23302-1-AP, Sanying, China) and VEGF (66828-1-Ig, Sanying, China) overnight at 4 °C.

Techniques: Western Blot, Labeling