human fibronectin elisa kit  (Advisains)

Journal: Biomedicines

Article Title: Is Spheroid a Relevant Model to Address Fibrogenesis in Keloid Research?

doi: 10.3390/biomedicines11092350

Figure Lengend Snippet: Overexpression of ECM related genes discontinues when KFs are cultured from 2D to 3D. Fibronectin expression was assessed and quantified using ELISA ( A ) and RT-qPCR ( B ) in monolayers and spheroids made with NDFs and KFs in the presence of TGF-β1 vs. control. Type I ( C ) and type III ( D ) collagen encoding mRNA, and COL1A1 / COL3A1 ratio ( E ) were evaluated by RT-qPCR in monolayers or spheroids produced and treated as previously described [ n = 4 spheroids per condition]. Statistical analyses were performed using two-way ANOVA * for p < 0.05; ** for p < 0.005; *** for p < 0.001; **** for p < 0.0001.

Article Snippet: After 7 days, concentration of α-SMA and fibronectin in 2D and 3D culture were quantified using Human α-SMA ELISA (ab240678, Abcam, Cambridge, United Kingdom) and Human fibronectin ELISA (ab219046, Abcam, Cambridge, United Kingdom) according to manufacturer instructions.

Techniques: Over Expression, Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Produced

Journal: International Journal of Molecular Sciences

Article Title: Derivation of Human Corneal Keratocytes from ReLEx SMILE Lenticules for Cell Therapy and Tissue Engineering

doi: 10.3390/ijms24108828

Figure Lengend Snippet: hCSC characteristics: ( A ) percentage of various fibroblast and keratocyte phenotype markers, adhesive molecule markers in two hCSC populations: CFs ( n = 13), rCFs ( n = 8); * p < 0.05, ** p = 0.003, # p = 0.01. ( B ) Percentage of CFs and rCFs that expressed keratocyte markers. ( C ) Fluorescence microscopy images showing the comparative expression of various markers in hCSCs: green—stained with fibronectin and α-smooth muscle α actin antibodies (induction of myofibroblasts with addition of TGFβ1, positive control), blue—stained with DAPI for nuclei. The scale bar is 100 µm for the fibronectin and 20 µm for the other markers.

Article Snippet: Commercially available ELISA kits were used to determine the human insulin growth factor-1 (IGF-1), human free brain-derived neurotrophic factor (BDNF) (all kits: R&D Systems, Minnesota, MN, USA), human stromal-derived factor-1a (SDF-1a; Thermo Scientific, Waltham, MA, USA), human vimentin, total collagen, human fibronectin (all kits: Abcam, Boston, MA, USA), human soluble intercellular adhesion molecule-1 (sICAM-1; Affymetrix eBioscience, Santa Clara, CA, USA), vascular endothelial growth factor (VEGF), erythropoietin (EPO), and tumor necrosis factor-alpha (TNF-α) (all kits: Vector-BEST, Novosibirsk, Russia), produced by the CFs and rCFs.

Techniques: Fluorescence, Microscopy, Expressing, Staining, Positive Control