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rat mcp1 elisa kit - 96 test  (Advisains)


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    Advisains rat mcp1 elisa kit - 96 test
    Rat Mcp1 Elisa Kit 96 Test, supplied by Advisains, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab219045/custom%40ab219045%4010%2E3389%2Ffcvm%2E2025%2E1631648?v=Advisains
    Average 99 stars, based on 38 article reviews
    rat mcp1 elisa kit - 96 test - by Bioz Stars, 2026-07
    99/100 stars

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    CCL2 protein is significantly elevated in the ipsilesional DRG at 12- and 24-hours post-injury (hpi) compared to naïve rats (*p=0.0092, 12hpi vs naïve, *p=0.0127 24hpi vs naïve) and is near naïve-levels by 72 hpi to 120 hpi (p>0.05) ( A , pg/ug total protein, n=6 per time-point). (8/16) rats demonstrated allodynia at 7 dpi, defined as a reduction of more than 30% from baseline paw withdrawal thresholds (B) . CCL2 remains near-naïve levels at 7days post-injury (dpi) and is not different in rats that do or do not have pain at that time point (C) . Dashed line indicates naïve. Group averages ± SEM are represented as bars and individual data points as open circles.

    Journal: bioRxiv

    Article Title: Acute or chronic depletion of macrophages in the dorsal root ganglia induces neuropathic pain after unilateral cervical spinal cord injury

    doi: 10.1101/2023.08.11.553038

    Figure Lengend Snippet: CCL2 protein is significantly elevated in the ipsilesional DRG at 12- and 24-hours post-injury (hpi) compared to naïve rats (*p=0.0092, 12hpi vs naïve, *p=0.0127 24hpi vs naïve) and is near naïve-levels by 72 hpi to 120 hpi (p>0.05) ( A , pg/ug total protein, n=6 per time-point). (8/16) rats demonstrated allodynia at 7 dpi, defined as a reduction of more than 30% from baseline paw withdrawal thresholds (B) . CCL2 remains near-naïve levels at 7days post-injury (dpi) and is not different in rats that do or do not have pain at that time point (C) . Dashed line indicates naïve. Group averages ± SEM are represented as bars and individual data points as open circles.

    Article Snippet: CCL2/MCP-1 levels in DRG tissue lysate were assayed using an ELISA kit (Abcam, MCP-1(CCL2) Rat ELISA kit #ab100777) as per the protocol and reported as pg/mg total protein.

    Techniques:

    CCL2, CCR2 and CD68 expression at 7 and 28dpi in the C7-8 ipsilesional dorsal root ganglia (DRG) normalized to housekeeping genes GAPDH and 18S in vehicle (n=17 7dpi, n=11 28dpi)- and INCB3344-treated (n=16 7dpi, n=13) rats, expressed as fold change over expression in naïve rats. CCL2 mRNA is significantly downregulated by 7 dpi in vehicle- and INCB3344-treated rats (***p<0.001, compared to naive) (A) and remains downregulated at 28dpi (***p<0.001 compared to naïve) (B) . CCR2 mRNA is significantly elevated at 7dpi (C) in both vehicle- and INCB3344-treated rats (*p=0.021 vehicle compared to naïve, *p=0.026 INCB3344 compared to naïve) and at 28 dpi (D) in both treatment groups (***p=0.001 vehicle compared to naïve, **p=0.004 INCB3344 compared to naive). By 7 dpi the expression of CD68 mRNA in the DRG (E) is significantly increased in vehicle (*p=0.039)- and INCB-treated rats (***p<0.001) compared to naive. At 28 dpi CD68 mRNA expression (F) is similarly significantly elevated for vehicle- and INCB-treated rats (***p<0.001 compared to naïve). Group averages ± SEM are represented as bars and individual data points as open circles.

    Journal: bioRxiv

    Article Title: Acute or chronic depletion of macrophages in the dorsal root ganglia induces neuropathic pain after unilateral cervical spinal cord injury

    doi: 10.1101/2023.08.11.553038

    Figure Lengend Snippet: CCL2, CCR2 and CD68 expression at 7 and 28dpi in the C7-8 ipsilesional dorsal root ganglia (DRG) normalized to housekeeping genes GAPDH and 18S in vehicle (n=17 7dpi, n=11 28dpi)- and INCB3344-treated (n=16 7dpi, n=13) rats, expressed as fold change over expression in naïve rats. CCL2 mRNA is significantly downregulated by 7 dpi in vehicle- and INCB3344-treated rats (***p<0.001, compared to naive) (A) and remains downregulated at 28dpi (***p<0.001 compared to naïve) (B) . CCR2 mRNA is significantly elevated at 7dpi (C) in both vehicle- and INCB3344-treated rats (*p=0.021 vehicle compared to naïve, *p=0.026 INCB3344 compared to naïve) and at 28 dpi (D) in both treatment groups (***p=0.001 vehicle compared to naïve, **p=0.004 INCB3344 compared to naive). By 7 dpi the expression of CD68 mRNA in the DRG (E) is significantly increased in vehicle (*p=0.039)- and INCB-treated rats (***p<0.001) compared to naive. At 28 dpi CD68 mRNA expression (F) is similarly significantly elevated for vehicle- and INCB-treated rats (***p<0.001 compared to naïve). Group averages ± SEM are represented as bars and individual data points as open circles.

    Article Snippet: CCL2/MCP-1 levels in DRG tissue lysate were assayed using an ELISA kit (Abcam, MCP-1(CCL2) Rat ELISA kit #ab100777) as per the protocol and reported as pg/mg total protein.

    Techniques: Expressing, Over Expression