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Advisains human il-17a elisa kit 1x96 test
Human Il 17a Elisa Kit 1x96 Test, supplied by Advisains, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 70 article reviews

human il-17a elisa kit 1x96 test - by Bioz Stars, 2026-07

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Isolation and characterization of macrophage-derived exosomes. A TEM image of exosomes isolated from the supernatant of iHvKp-stimulated macrophages or control group, scale bar, 100 nm. B NTA analysis of the isolated exosomes, including iHvKp-exo and control group. C Analysis of exosome markers ALIX, TSG101, and CD63 in the exosome preparation by western blot. D Levels of lipopolysaccharide (LPS), a bacterial cell membrane component, in the extracted exosomes, including iHvKp-exo and control group. The data are presented as mean ± SEM

Journal: Cellular & Molecular Biology Letters

Article Title: Exosomal miR-155-5p drives widespread macrophage M1 polarization in hypervirulent Klebsiella pneumoniae -induced acute lung injury via the MSK1/p38-MAPK axis

doi: 10.1186/s11658-023-00505-1

Figure Lengend Snippet: Isolation and characterization of macrophage-derived exosomes. A TEM image of exosomes isolated from the supernatant of iHvKp-stimulated macrophages or control group, scale bar, 100 nm. B NTA analysis of the isolated exosomes, including iHvKp-exo and control group. C Analysis of exosome markers ALIX, TSG101, and CD63 in the exosome preparation by western blot. D Levels of lipopolysaccharide (LPS), a bacterial cell membrane component, in the extracted exosomes, including iHvKp-exo and control group. The data are presented as mean ± SEM

Article Snippet: The following antibodies were used: anti-CD63 (1:500, Cat.ab216130, Abcam), anti-ALIX (1:2000, Cat.E6P9B, CST), anti-TSG101 (1:2000, Cat.ab125011, Abcam), anti-MSK1 (1:500, Cat.3489S, CST), anti-DUSP1 (1:1000, Cat.ab138265, Abcam), anti-p-p38 MAPK (1:1000, Cat.4511T, CST), anti-p38 MAPK (1:1000, Cat.8690S, CST), and anti-GAPDH (1:3000, Cat.92310SF, CST).

Techniques: Isolation, Derivative Assay, Western Blot

a Schematic diagram of construction of HTG HFHC mice model. b – f Records for the body weight ( P < 0.0001 by 2 tailed t test) ( b ), liver weight and the ratio of liver weight/body weight (%) (LW/BW) ( P < 0.0001 by 2 tailed t test) ( c ), fasting blood glucose levels ( P < 0.0001 by 2 tailed t test) ( d ), fasting insulin levels and homoeostasis model assessment of insulin resistance (HOMA-IR) index ( P < 0.0001 by 2 tailed t test) ( e ) and liver triglyceride (TG), non-esterified fatty acid (NEFA) and total cholesterol (TC) ( P < 0.0001 by 2 tailed t test) ( f ) in indicated groups ( n = 15 per group). g Pearson correlation analyses indicating the correlations between fasting blood glucose and ratio of LW/BW, and liver NEFA contents and fasting insulin levels in indicated groups ( n = 15 per parameter; n = 45 in total). h , i Representative pictures of H&E staining, NAS score ( P < 0.0001 by 2 tailed t test) ( h ) and oil red O staining ( P < 0.0001 by 2 tailed t test) ( i ) in indicated groups (scale bars: magnification, ×100; n = 10 per group). ( j, k ) Immunohistochemical assay showing the F4/80 or CD11b expression (scale bars: magnification, ×200) ( P < 0.0001 by 2 tailed t test) ( j ) and masson staining and sirius red staining (scale bars: magnification, 100×) ( P < 0.0001 by 2 tailed t test) ( k ) -indicated liver histopathologic changes in liver of indicated groups ( n = 10 per group). l Representative inflammation-related cytokines and genes expression profiles including interleukin-6 (IL-6) ( P = 0.0002 by 2 tailed t test), tumour necrosis factor-α (TNF-α) ( P < 0.0001 by 2 tailed t test), interleukin-1β (IL-1β) ( P = 0.0054 by 2 tailed t test), interleukin-18 (IL-18) ( P = 0.0015 by 2 tailed t test), chemokine CCL2 ( P = 0.0053 by 2 tailed t test) and interleukin-10 (IL-10) ( P < 0.0001 by 2 tailed t test) in serum ( n = 15 mice per group) or livers ( n = 5 per group; P < 0.0001 by 2 tailed t test) from indicated groups. m , n Representative mRNA levels of fatty acid metabolism ( P < 0.01 by 2 tailed t test) ( m )- and profibrotic factors ( P < 0.01 by 2 tailed t test) ( n )-associated genes expression in livers from indicated groups ( n = 5 per group). o Representative liver function-related indicators including alanine transaminase (ALT) ( P < 0.0001 by 2 tailed t test), aspartate aminotransferase (AST) ( P < 0.0001 by 2 tailed t test), alkline phosphatase (AKP) ( P = 0.0019 by 2 tailed t test) and glutamyl transpeptidase (GGT) ( P = 0.0449 by 2 tailed t test) in serum from indicated groups ( n = 15 per group). Data are expressed as mean ± SEM. The relevant experiments presented in this part were performed independently at least three times. P < 0.05 indicates statistical significance.

Journal: Nature Communications

Article Title: Tripartite motif containing 26 prevents steatohepatitis progression by suppressing C/EBPδ signalling activation

doi: 10.1038/s41467-023-42040-9

Figure Lengend Snippet: a Schematic diagram of construction of HTG HFHC mice model. b – f Records for the body weight ( P < 0.0001 by 2 tailed t test) ( b ), liver weight and the ratio of liver weight/body weight (%) (LW/BW) ( P < 0.0001 by 2 tailed t test) ( c ), fasting blood glucose levels ( P < 0.0001 by 2 tailed t test) ( d ), fasting insulin levels and homoeostasis model assessment of insulin resistance (HOMA-IR) index ( P < 0.0001 by 2 tailed t test) ( e ) and liver triglyceride (TG), non-esterified fatty acid (NEFA) and total cholesterol (TC) ( P < 0.0001 by 2 tailed t test) ( f ) in indicated groups ( n = 15 per group). g Pearson correlation analyses indicating the correlations between fasting blood glucose and ratio of LW/BW, and liver NEFA contents and fasting insulin levels in indicated groups ( n = 15 per parameter; n = 45 in total). h , i Representative pictures of H&E staining, NAS score ( P < 0.0001 by 2 tailed t test) ( h ) and oil red O staining ( P < 0.0001 by 2 tailed t test) ( i ) in indicated groups (scale bars: magnification, ×100; n = 10 per group). ( j, k ) Immunohistochemical assay showing the F4/80 or CD11b expression (scale bars: magnification, ×200) ( P < 0.0001 by 2 tailed t test) ( j ) and masson staining and sirius red staining (scale bars: magnification, 100×) ( P < 0.0001 by 2 tailed t test) ( k ) -indicated liver histopathologic changes in liver of indicated groups ( n = 10 per group). l Representative inflammation-related cytokines and genes expression profiles including interleukin-6 (IL-6) ( P = 0.0002 by 2 tailed t test), tumour necrosis factor-α (TNF-α) ( P < 0.0001 by 2 tailed t test), interleukin-1β (IL-1β) ( P = 0.0054 by 2 tailed t test), interleukin-18 (IL-18) ( P = 0.0015 by 2 tailed t test), chemokine CCL2 ( P = 0.0053 by 2 tailed t test) and interleukin-10 (IL-10) ( P < 0.0001 by 2 tailed t test) in serum ( n = 15 mice per group) or livers ( n = 5 per group; P < 0.0001 by 2 tailed t test) from indicated groups. m , n Representative mRNA levels of fatty acid metabolism ( P < 0.01 by 2 tailed t test) ( m )- and profibrotic factors ( P < 0.01 by 2 tailed t test) ( n )-associated genes expression in livers from indicated groups ( n = 5 per group). o Representative liver function-related indicators including alanine transaminase (ALT) ( P < 0.0001 by 2 tailed t test), aspartate aminotransferase (AST) ( P < 0.0001 by 2 tailed t test), alkline phosphatase (AKP) ( P = 0.0019 by 2 tailed t test) and glutamyl transpeptidase (GGT) ( P = 0.0449 by 2 tailed t test) in serum from indicated groups ( n = 15 per group). Data are expressed as mean ± SEM. The relevant experiments presented in this part were performed independently at least three times. P < 0.05 indicates statistical significance.

Article Snippet: The TNF-α (Catalogue Number: ab100747), IL-1β (Catalogue Number: ab197742), IL-6 (Catalogue Number: ab222503), CCL2 (Catalogue Number: ab208979), IL-18 (Catalogue Number: ab216165), and insulin (Catalogue Number: ab285341) enzyme-linked immunosorbent assay (ELISA) kits were acquired from Abcam.

Techniques: Staining, Immunohistochemistry, Expressing

Mitochondrial damage and lung injury induced by MV in mice. Mice were ventilated for 2 or 4 h, and lung tissues were evaluated. A, Western blot showed that sirt1 decreased while p66shc and p-p66shc increased in a time-dependent manner after ventilation. B, Expression of VDAC1, PINK1, and Parkin after MV was determined by Western blot. C, Expression of cell junction proteins after MV. D, Analysis of mitochondrial respiration of isolated lung tissue. MMR and RRC decreased after MV. E–F, MDA increased while SOD decreased after MV. H, Lung W/D ratio. I, The pathological changes in mice with or without high tidal volume ventilation (magnification 400×; scale bar, 5 μm). * P < 0.05 vs control group. Experiments were repeated at least three times.

Journal: Medicine and Science in Sports and Exercise

Article Title: Aerobic Exercise in Male Mice Prevents Ventilator-Induced Lung Injury by Inhibiting Mitochondrial Damage from sirt1 Dysregulation

doi: 10.1249/MSS.0000000000003203

Figure Lengend Snippet: Mitochondrial damage and lung injury induced by MV in mice. Mice were ventilated for 2 or 4 h, and lung tissues were evaluated. A, Western blot showed that sirt1 decreased while p66shc and p-p66shc increased in a time-dependent manner after ventilation. B, Expression of VDAC1, PINK1, and Parkin after MV was determined by Western blot. C, Expression of cell junction proteins after MV. D, Analysis of mitochondrial respiration of isolated lung tissue. MMR and RRC decreased after MV. E–F, MDA increased while SOD decreased after MV. H, Lung W/D ratio. I, The pathological changes in mice with or without high tidal volume ventilation (magnification 400×; scale bar, 5 μm). * P < 0.05 vs control group. Experiments were repeated at least three times.

Article Snippet: The primary antibodies were as follows: sirt1 (1:1000, ab189494; Abcam), p66shc (1:1000, ab33770; Abcam), p-p66shc (1:1000, ab54518; Abcam), PINK1 (1:1000, ab216144; Abcam), Parkin (1:1000, ab77924; Abcam), VDAC1 (1:1000, ab14734; Abcam), Caspase-3 (1:500, sc-7272; Santa Cruz), Occludin (1:1000, ab216327; Abcam), p120 (1:200, sc-23873; Santa Cruz), and ZO-1 (1:1000, ab96587; Abcam).

Techniques: Western Blot, Expressing, Isolation

Aerobic exercise reduces VILI. Mice were divided into four groups: control group, MV group (ventilation for 4 h), EX group (exercise training for 5 wk), and EX + MV group (ventilation for 4 h after 5 wk of moderate-intensity exercise training). A, Experimental protocol of aerobic exercise. B, Western blot showed that exercise training could reverse the effects of MV on sirt1, p66shc, p-p66shc, and PINK1. C, Cell junction protein expression. D, Cytokine levels in BALF. E, Lung W/D ratio. F, Pathological changes of lung tissues were observed via H&E (magnification 400×; scale bar, 5 μm). * P < 0.05 vs control group; # P < 0.05 vs MV 4-h group. Experiments were repeated at least three times.

Journal: Medicine and Science in Sports and Exercise

Article Title: Aerobic Exercise in Male Mice Prevents Ventilator-Induced Lung Injury by Inhibiting Mitochondrial Damage from sirt1 Dysregulation

doi: 10.1249/MSS.0000000000003203

Figure Lengend Snippet: Aerobic exercise reduces VILI. Mice were divided into four groups: control group, MV group (ventilation for 4 h), EX group (exercise training for 5 wk), and EX + MV group (ventilation for 4 h after 5 wk of moderate-intensity exercise training). A, Experimental protocol of aerobic exercise. B, Western blot showed that exercise training could reverse the effects of MV on sirt1, p66shc, p-p66shc, and PINK1. C, Cell junction protein expression. D, Cytokine levels in BALF. E, Lung W/D ratio. F, Pathological changes of lung tissues were observed via H&E (magnification 400×; scale bar, 5 μm). * P < 0.05 vs control group; # P < 0.05 vs MV 4-h group. Experiments were repeated at least three times.

Techniques: Western Blot, Expressing

CS-induced mitochondrial damage and degradation of junction proteins. HLMVEC cell monolayers were subjected to CS for 2 or 4 h. A and B, Expression of p66shc, p-p66shc, caspase-3, VDAC1, PINK1, and Parkin was detected by Western blot. C and D, MMP and ROS production were detected by flow cytometry. E, Mitochondrial respiration was analyzed by O2K. F, Representative electron microscopy images of the three groups (scale bars, 1 μm). G, Mitochondrial imaging of living HLMVEC cells. H and I, After HLMVEC CS, MDA increased and SOD decreased. J, Cell junction proteins expression. * P < 0.05 vs control. Experiments were repeated at least three times.

Journal: Medicine and Science in Sports and Exercise

Article Title: Aerobic Exercise in Male Mice Prevents Ventilator-Induced Lung Injury by Inhibiting Mitochondrial Damage from sirt1 Dysregulation

doi: 10.1249/MSS.0000000000003203

Figure Lengend Snippet: CS-induced mitochondrial damage and degradation of junction proteins. HLMVEC cell monolayers were subjected to CS for 2 or 4 h. A and B, Expression of p66shc, p-p66shc, caspase-3, VDAC1, PINK1, and Parkin was detected by Western blot. C and D, MMP and ROS production were detected by flow cytometry. E, Mitochondrial respiration was analyzed by O2K. F, Representative electron microscopy images of the three groups (scale bars, 1 μm). G, Mitochondrial imaging of living HLMVEC cells. H and I, After HLMVEC CS, MDA increased and SOD decreased. J, Cell junction proteins expression. * P < 0.05 vs control. Experiments were repeated at least three times.

Techniques: Expressing, Western Blot, Flow Cytometry, Electron Microscopy, Imaging

Effects of aerobic exercise on mitochondrial damage and cell permeability after CS. AICAR was used 24 h before stretching to simulate the effect of aerobic exercise in mice. A and G, The expression of VDAC1, p66shc, PINK1, p120, and Occludin was determined by Western blot. B and C, MMP and ROS production were detected by FCM. D, Mitochondrial respiration in each group of HMLVEC was analyzed by O2K. E, Electron microscopy images of HLMVEC cells (scale bars, 1 μm). F, Mitochondrial imaging of living HLMVEC cells. H, The colocalization of p120 ( green ) and VE-cadherin ( red ) in HLMVEC cells was determined by immunofluorescence. Nuclei were stained with DAPI ( blue ). Scale bar, 10 μm. I, Immunofluorescence was used to detect the distribution of F-actin ( green ) and p120 ( red ). Nuclei were stained with DAPI ( blue ). Scale bar, 10 μm. * P < 0.05 vs control; # P < 0.05 vs CS 4-h group. Experiments were repeated at least three times.

Journal: Medicine and Science in Sports and Exercise

Article Title: Aerobic Exercise in Male Mice Prevents Ventilator-Induced Lung Injury by Inhibiting Mitochondrial Damage from sirt1 Dysregulation

doi: 10.1249/MSS.0000000000003203

Figure Lengend Snippet: Effects of aerobic exercise on mitochondrial damage and cell permeability after CS. AICAR was used 24 h before stretching to simulate the effect of aerobic exercise in mice. A and G, The expression of VDAC1, p66shc, PINK1, p120, and Occludin was determined by Western blot. B and C, MMP and ROS production were detected by FCM. D, Mitochondrial respiration in each group of HMLVEC was analyzed by O2K. E, Electron microscopy images of HLMVEC cells (scale bars, 1 μm). F, Mitochondrial imaging of living HLMVEC cells. H, The colocalization of p120 ( green ) and VE-cadherin ( red ) in HLMVEC cells was determined by immunofluorescence. Nuclei were stained with DAPI ( blue ). Scale bar, 10 μm. I, Immunofluorescence was used to detect the distribution of F-actin ( green ) and p120 ( red ). Nuclei were stained with DAPI ( blue ). Scale bar, 10 μm. * P < 0.05 vs control; # P < 0.05 vs CS 4-h group. Experiments were repeated at least three times.

Techniques: Permeability, Expressing, Western Blot, Electron Microscopy, Imaging, Immunofluorescence, Staining

The regulating role of p66shc and PINK1 on mitochondria and cell junction proteins in VILI model. HLMVEC cells were cyclically stretched after transfection with p66shc siRNA or Pink1 siRNA. A and B, Expression of adherens junction and tight junction proteins after transfection with p66shc or Pink1 shRNA. C, MMR and RRC of HLMVEC cells increased after p66shc deletion, whereas MMR and RRC of HLMVEC cells decreased after PINK1 deletion. * P < 0.05 vs control; # P < 0.05 vs CS 4-h group. Experiments were repeated at least three times.

Journal: Medicine and Science in Sports and Exercise

Article Title: Aerobic Exercise in Male Mice Prevents Ventilator-Induced Lung Injury by Inhibiting Mitochondrial Damage from sirt1 Dysregulation

doi: 10.1249/MSS.0000000000003203

Figure Lengend Snippet: The regulating role of p66shc and PINK1 on mitochondria and cell junction proteins in VILI model. HLMVEC cells were cyclically stretched after transfection with p66shc siRNA or Pink1 siRNA. A and B, Expression of adherens junction and tight junction proteins after transfection with p66shc or Pink1 shRNA. C, MMR and RRC of HLMVEC cells increased after p66shc deletion, whereas MMR and RRC of HLMVEC cells decreased after PINK1 deletion. * P < 0.05 vs control; # P < 0.05 vs CS 4-h group. Experiments were repeated at least three times.

Techniques: Transfection, Expressing, shRNA

Sirt1 in VILI. Mice were injected with a complex composed of transfection reagent and sirt1 siRNA. Mice without ventilation were used as controls. A, Successful sirt1 siRNA transfection was determined by Western blot. B and C, Sirt1 KD significantly increased the expression of p66shc and p-p66shc while reducing the expression of PINK1, p120, and Occludin in lung tissue. D, Sirt1 KD significantly inhibited MMR and RRC in mice lung tissues. E, IL-1β, IL-6, and IL-10 in BALF were detected by enzyme-linked immunosorbent assay. F, Lung W/D ratio. G, H&E staining of mouse lung (magnification 400×; scale bar, 5 μm). * P < 0.05 vs the control group; # P < 0.05 vs MV 4-h group; & P < 0.05 vs sirt1 KD group. Experiments were repeated at least three times.

Journal: Medicine and Science in Sports and Exercise

Article Title: Aerobic Exercise in Male Mice Prevents Ventilator-Induced Lung Injury by Inhibiting Mitochondrial Damage from sirt1 Dysregulation

doi: 10.1249/MSS.0000000000003203

Figure Lengend Snippet: Sirt1 in VILI. Mice were injected with a complex composed of transfection reagent and sirt1 siRNA. Mice without ventilation were used as controls. A, Successful sirt1 siRNA transfection was determined by Western blot. B and C, Sirt1 KD significantly increased the expression of p66shc and p-p66shc while reducing the expression of PINK1, p120, and Occludin in lung tissue. D, Sirt1 KD significantly inhibited MMR and RRC in mice lung tissues. E, IL-1β, IL-6, and IL-10 in BALF were detected by enzyme-linked immunosorbent assay. F, Lung W/D ratio. G, H&E staining of mouse lung (magnification 400×; scale bar, 5 μm). * P < 0.05 vs the control group; # P < 0.05 vs MV 4-h group; & P < 0.05 vs sirt1 KD group. Experiments were repeated at least three times.

Techniques: Injection, Transfection, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Staining

Journal: Biomedicines

Article Title: Neuroprotective Effects of Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell Extracellular Vesicles in Ischemic Stroke Models

doi: 10.3390/biomedicines11092550

Figure Lengend Snippet: Characterization of hiPS-MSCs derived extracellular vesicles (hiPS-MSCs-EV) ( A ). Flowchart of hiPS-MSC-EVs isolation from the culture medium. ( B ). Morphology of hiPS-MSC-EVs under transmission electron microscopy. ( C ). Representative bioanalyzer profile of the RNA contained in hiPS-MSC-EVs. ( D ). Western blotting analysis of exosomal positive markers CD63, CD9, and CD81 and negative marker Calnexin in MSC lysate and hiPS-MSC-EVs. Tubulin was used as the loading control. ( E ). Flow cytometry analysis of positive marker CD63 expression after binding to CD9 and CD81 antibody-coated beads. The solid gray line represents cells that were unstained. while solid pink line represents the experimental samples. ( F ). Particle size distribution of iPSC-MSC-EVs measured using a flow nanoanalyzer.

Article Snippet: Antibodies against CD9 (1:200; sc-13118, Santa Cruz, CA, USA), CD63 (1:200; ab216130, Abcam, Cambridge, UK), and CD81 (1:100; sc-166029, Santa Cruz), Calnexin (1:200; sc-23954, SantaCruz), Tubulin (#15115, Cell Signaling Technology, Danvers, MA, USA) were used to target the exosomes.

Techniques: Derivative Assay, Isolation, Transmission Assay, Electron Microscopy, Western Blot, Marker, Flow Cytometry, Expressing, Binding Assay

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