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Journal: Nucleic Acids Research
Article Title: De-dimerization of PTB is catalyzed by PDI and is involved in the regulation of p53 translation
doi: 10.1093/nar/gkab708
Figure Lengend Snippet: Regulation of PTB dimerization by PDI. ( A ) The expression level of indicated proteins treated with siRNAs against NC or PDI were analyzed by non-reducing SDS-PAGE. β-Actin was used as the loading control. ( B ) The expression level of indicated proteins with over expression of PDI were analyzed by non-reducing SDS-PAGE. β-Actin was used as the loading control. ( C ) Schematic illustration of PDI domain structures. The CGHC active sites were indicated above. ( D ) Expression of PTB dimer and monomer with over expression of PDI treated with NADP or NADPH at indicated concentrations for 12 h were analyzed by non-reducing SDS-PAGE and quantified below. GAPDH was used as the loading control. ( E ) Expression of PTB with over expression of PDI WT and PDI mutants were analyzed under non-reducing SDS-PAGE and quantified below. GAPDH was used as the loading control.
Article Snippet:
Techniques: Expressing, SDS Page, Control, Over Expression
Journal: Nucleic Acids Research
Article Title: De-dimerization of PTB is catalyzed by PDI and is involved in the regulation of p53 translation
doi: 10.1093/nar/gkab708
Figure Lengend Snippet: Regulation of PTB dimerization by PDI. ( A ) The expression level of indicated proteins treated with siRNAs against NC or PDI were analyzed by non-reducing SDS-PAGE. β-Actin was used as the loading control. ( B ) The expression level of indicated proteins with over expression of PDI were analyzed by non-reducing SDS-PAGE. β-Actin was used as the loading control. ( C ) Schematic illustration of PDI domain structures. The CGHC active sites were indicated above. ( D ) Expression of PTB dimer and monomer with over expression of PDI treated with NADP or NADPH at indicated concentrations for 12 h were analyzed by non-reducing SDS-PAGE and quantified below. GAPDH was used as the loading control. ( E ) Expression of PTB with over expression of PDI WT and PDI mutants were analyzed under non-reducing SDS-PAGE and quantified below. GAPDH was used as the loading control.
Article Snippet: NADP (ab146316) and
Techniques: Expressing, SDS Page, Over Expression