Journal: eLife

Article Title: Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system

doi: 10.7554/eLife.85596

Figure Lengend Snippet: These depictions are based on the localization patterns observed in — . Shown are the observed localization patterns for MVP, FUNDC2, PSMA3, PSMG2, BAG5, and SAMM50.

Article Snippet: Rabbit polyclonal anti-PACRG (ab4090), rabbit polyclonal anti-SPATA18 (180154), mouse monoclonal anti-MVP (ab14562), and rabbit polyclonal anti-PSMG2 (ab172909) were purchased from Abcam, Cambridge, United Kingdom.

Techniques:

Journal: Journal of Cancer

Article Title: Lovastatin Inhibits Cancer Stem Cells and Sensitizes to Chemo- and Photodynamic Therapy in Nasopharyngeal Carcinoma

doi: 10.7150/jca.19100

Figure Lengend Snippet: Statins inhibit the stemness properties of NPC sphere-forming cells. (A, B) NPC sphere-forming cells were cultured in ultra-low attachment plates in the presence of different statins (1 µM) in stem cell culture medium. (A) Photomicrographic images were taken at the end of the 72-h culture period. Original magnification: ×200. (B) Sphere formation was quantified by multiplying the number of tumorspheres and the surface areas of the tumorspheres in each well. (C) The sphere-forming cells were treated with different concentrations of LV for 48 h and subjected to Western blot analysis. LV, lovastatin; AV, atorvastatin; SV, simvastatin.

Article Snippet: Statins were purchased from Abcam (atorvastatin [ab145604], lovastatin [ab120614], and simvastatin [ab120505]) or Sigma-Aldrich (St Louis, MO, USA) and dissolved in DMSO as a 20 ~ 30 mM stock solution and stored at -20°C before use.

Techniques: Cell Culture, Stem Cell Culture, Western Blot