dithiothreitol  (Advisains)

Journal: iScience

Article Title: Characterization of an in vitro steatosis model simulating activated de novo lipogenesis in MAFLD patients

doi: 10.1016/j.isci.2023.107727

Figure Lengend Snippet: The effect of C75 and JNK-IN-5A on DNL and OA steatosis models (A and C) TAG accumulation and cell viability is measured after the treatment of OA and DNL steatosis models with 10 μM JNK-IN-5A and 20 μM C75. Data are represented as mean ± SD, ∗p < 0.05, Student’s t test. (B and D) Western blot analysis for the proteins involved in the de novo lipogenesis after treatment of the OA and DNL steatosis models with 10 μM JNK-IN-5A and 40 μM C75. Data are represented as mean ± SD, ∗p < 0.05, Student’s t test. (E) Fluorescence staining of lipid droplet and cytoskeleton. Green: neutral lipids, Red: Cytoskeleton (F-actin), Scale bar, 100 μm. ROI of GFP intensity is measured with ImageJ. Data are represented as mean ± SD, ∗p < 0.05, Student’s t test.

Article Snippet: JNK-IN-5a (HY-15881, MCE) and C75 (ab141397, abcam) were dissolved into DMSO.

Techniques: Western Blot, Fluorescence, Staining

Journal: iScience

Article Title: Characterization of an in vitro steatosis model simulating activated de novo lipogenesis in MAFLD patients

doi: 10.1016/j.isci.2023.107727

Figure Lengend Snippet:

Article Snippet: JNK-IN-5a (HY-15881, MCE) and C75 (ab141397, abcam) were dissolved into DMSO.

Techniques: Recombinant, Cell Counting, Software

Journal: iScience

Article Title: Characterization of an in vitro steatosis model simulating activated de novo lipogenesis in MAFLD patients

doi: 10.1016/j.isci.2023.107727

Figure Lengend Snippet:

Article Snippet: C75 , abcam , Catalog #: ab141397.

Techniques: Recombinant, Cell Counting, Software