anti-fas antibody  (Advisains)

Journal: The Journal of Clinical Investigation

Article Title: Initial productive and latent HIV infections originate in vivo by infection of resting T cells

doi: 10.1172/JCI171501

Figure Lengend Snippet: Fiebig I demographics, CD4 + T cell counts, plasma viral load, and clade at diagnosis and biopsy

Article Snippet: In the double-label phenotyping analyses, following the ELF97 step, tissues sections were twice washed in TBS for 5 minutes and blocked by background Sniper (BS966L, Biocare Medical) for 45 minutes, before incubating the tissue sections at 4°C overnight with anti-CD4 antibody (1:25 dilution, ab133616, Abcam) or CD25 (4C9) (1:20 dilution, 125M-16, Cell Marque).

Techniques:

Journal: The Journal of Clinical Investigation

Article Title: Initial productive and latent HIV infections originate in vivo by infection of resting T cells

doi: 10.1172/JCI171501

Figure Lengend Snippet: ISH with ELF97 substrate identifies HIV-producing cells as cells with green virions visible against diffusely stained HIV intracellular RNA. Nuclei were counterstained blue with DAPI. ( A ) Example of a single productively infected cell. Arrow points to visible HIV virions. ( B ) Arrow points to visible virions shared by 2 cells, consistent with cell-to-cell spread. ( C and D ) HIV-producing cells are CD4 + . Virions and intracellular RNA, green; CD4, red. ( C ) HIV-producing CD4 + cell with red/yellow CD4 staining visible at the cell’s margins. ( D ) Double-labeled red/yellow HIV-producing cell. ( E ) HIV-producing cells are CD25 – . Virions and intracellular RNA, green; CD25, red; DAPI counterstain. Two HIV-producing (arrows) cell-CD25 – conjugates with visible HIV virions shared by the 2 cells (arrows) in a field with a CD25 + HIV – cell. Scale bar: 10 mm. ( F ) Whisker plot of data in from 5 participants, with percentages of HIV virus + producing CD25 – cells (red; average 99.9%, range 99.4%–100%) and virus-uninfected CD25 – cells (green; average 98.3%, range 97.5%–100%). Scale bar: 10 µm.

Article Snippet: In the double-label phenotyping analyses, following the ELF97 step, tissues sections were twice washed in TBS for 5 minutes and blocked by background Sniper (BS966L, Biocare Medical) for 45 minutes, before incubating the tissue sections at 4°C overnight with anti-CD4 antibody (1:25 dilution, ab133616, Abcam) or CD25 (4C9) (1:20 dilution, 125M-16, Cell Marque).

Techniques: Staining, Infection, Labeling, Whisker Assay

Journal: The Journal of Clinical Investigation

Article Title: Initial productive and latent HIV infections originate in vivo by infection of resting T cells

doi: 10.1172/JCI171501

Figure Lengend Snippet: LNMNCs were either fixed immediately or grown for 2 days with CD3/CD28 beads and then fixed and pelleted. After Histogel added to the fixed cell pellet had solidified, the pellets were embedded in paraffin and sectioned and reacted with antibodies against CD4, CDK9, and cyclin T1. ( A and B ) CD4, blue; ( C and D ) CDK9, red; and ( E and F ) cyclin T1, green. ( G and H ) In the merged images, both before and after activation, pTEFb components CDK9 and cyclin T1 were detected as yellow- to orange-appearing nuclei in blue CD4 + T cells. Scale bar: 10 mm. ( I ) Whisker plot of data from of pTEFb expression in CD4 + T cells in LN tissue sections from 5 participants in Fiebig I (average percentage, 76.7%; range, 72.1%–81%) and 2 individuals without HIV infection (average percentage, 84%; range, 82.5%–87.4%). Scale bar: 10 µm.

Article Snippet: In the double-label phenotyping analyses, following the ELF97 step, tissues sections were twice washed in TBS for 5 minutes and blocked by background Sniper (BS966L, Biocare Medical) for 45 minutes, before incubating the tissue sections at 4°C overnight with anti-CD4 antibody (1:25 dilution, ab133616, Abcam) or CD25 (4C9) (1:20 dilution, 125M-16, Cell Marque).

Techniques: Activation Assay, Whisker Assay, Expressing, Infection

Journal: The Journal of Clinical Investigation

Article Title: Initial productive and latent HIV infections originate in vivo by infection of resting T cells

doi: 10.1172/JCI171501

Figure Lengend Snippet: ( A – H ) Participant ID (PID) 2563 and 2585. ( A and E ) White arrows track red HIV RNA + cells in ( B and F ) green CD4 + cells with blue cyclin T1 + and ( C and G ) magenta CDK9 + nuclei. ( B and F ) Green-outlined blue arrows point to uninfected CD4 + T cells with cyclin T1 + nuclei. ( C and G ) Outlined magenta arrows point to CDK9 + nuclei in uninfected cells. ( D and H ) HIV RNA + cells (white arrows) are green CD4 + cyclin T1 + and CDK9 + . Green outlined arrows point to uninfected cells with the same phenotype. ( I and J ) PID 2451. White arrows in the boxed region point to red HIV RNA + cells in green CD4 + T cells with magenta CDK9 + and blue cyclin T1 + nuclei. Green outlined arrows point to uninfected CD4 + CDK9 + cyclin 1 + T cells. The outlined white arrow points to an uninfected CD4 + T cell whose nucleus is pTEFb – (CDK9 – cyclin T1 – ). Scale bar: 10 μm.

Article Snippet: In the double-label phenotyping analyses, following the ELF97 step, tissues sections were twice washed in TBS for 5 minutes and blocked by background Sniper (BS966L, Biocare Medical) for 45 minutes, before incubating the tissue sections at 4°C overnight with anti-CD4 antibody (1:25 dilution, ab133616, Abcam) or CD25 (4C9) (1:20 dilution, 125M-16, Cell Marque).

Techniques:

Journal: The Journal of Clinical Investigation

Article Title: Initial productive and latent HIV infections originate in vivo by infection of resting T cells

doi: 10.1172/JCI171501

Figure Lengend Snippet: ( A ) Low-magnification (original magnification, ×10) view of green HIV-producing cells in LN tissues after 24 months of ART initiated in Fiebig I. The rectangle encloses 6 HIV + cells. Scale bar: 100 μm. ( B ) HIV RNA in the cell and cell fragments and in HIV virions is shown in green, and CD4 + cells are red with blue nuclei. The cell within the rectangle shows orange staining areas, indicating CD4 + , and is surrounded by virions and clumps of cell-associated virions. Field of CD4 + CD25 – cells except for 1 clearly CD4 + CD25 + cell (white arrow). Scale bar: 20 μm. ( C ) Whisker plot of data from showing that an average of 98.8% (range, 98.1%–100%) of the HIV-producing cells in 4 participants who initiated ART in Fiebig I were CD25 – .

Article Snippet: In the double-label phenotyping analyses, following the ELF97 step, tissues sections were twice washed in TBS for 5 minutes and blocked by background Sniper (BS966L, Biocare Medical) for 45 minutes, before incubating the tissue sections at 4°C overnight with anti-CD4 antibody (1:25 dilution, ab133616, Abcam) or CD25 (4C9) (1:20 dilution, 125M-16, Cell Marque).

Techniques: Staining, Whisker Assay

Journal: Signal Transduction and Targeted Therapy

Article Title: Anti-PD-1 antibody armored γδ T cells enhance anti-tumor efficacy in ovarian cancer

doi: 10.1038/s41392-023-01646-7

Figure Lengend Snippet: Lv-PD1-γδ T cells have no potential tumorigenicity. a Imaging of the growth status of nude mice subcutaneously injected with NCI-H520 (8 × 10 6 cells/mouse), 1× PBS (50 μL), high-dose γδ T cells (1 × 10 9 cells/mouse), medium-dose Lv-PD1-γδ T cells (1 × 10 7 cells/mouse), or high-dose Lv-PD1-γδ T cells (1 × 10 9 cells/mouse) in the 12th week ( n = 5). b Curves of tumor growth and quantitative statistical analysis of tumor volume over 12 weeks. Data are represented as the mean ± SEM. ** p < 0.01, by multigroup t test statistical analysis. c We recorded the body weight of nude mice before and after transplantation at the final time point. d Survival curve and statistical analysis within 120 days, using a log-rank (Mantel‒Cox) test with a 95% CI. e Hematoxylin-eosin staining of the subcutaneous injection site in nude mice. Scale bars, 25 μm. f , g Immunohistochemistry for CEA ( f ) and NSE ( g ) in nude mice after subcutaneous injection. Scale bars, 25 μm

Article Snippet: E9), anti-CEA antibody [EPCEAR7] (Abcam ab133633), and anti-NSE antibody [EPR3377] (Abcam ab79757) as primary antibodies.

Techniques: Imaging, Injection, Transplantation Assay, Staining, Immunohistochemistry