Journal: mAbs
Article Title: Modifying antibody-FcRn interactions to increase the transport of antibodies through the blood-brain barrier
doi: 10.1080/19420862.2023.2229098
Figure Lengend Snippet: O4-YTE hIgG1 antibodies persist in the brain for up to 504 hours after dosing. (a) Representative images of brains dissected from WT CD-1 mice at 2, 4, 6, 12, 24, 48, 96, 168, 336, and 504 hours (h) after administration of O4-YTE hIgG1 test articles and immunostained for human IgGκ chain. (b) Quantification of human IgGκ immunostaining in brains from groups represented in panel a. Four matching sections along the rostral-caudal axis were selected and quantified for each of two animals per group. (c) Quantification of hIgG protein in the CSF and (d) serum of animals from groups represented in panel a. Antibody concentration was estimated with a sandwich ELISA assay using antibodies specific for hIgG protein. Error bars represent SEM.
Article Snippet: Antibody concentrations in murine serum and CSF were measured with a human IgG ELISA kit (Abcam, #195215) following manufacturer’s standard protocol.
Techniques: Immunostaining, Concentration Assay, Sandwich ELISA