human il-17 elisa kit 96 tests  (Advisains)

Journal: International Journal of Biological Sciences

Article Title: Downregulation of miR-199a-3p mediated by the CtBP2-HDAC1-FOXP3 transcriptional complex contributes to acute lung injury by targeting NLRP1

doi: 10.7150/ijbs.37133

Figure Lengend Snippet: The proinflammatory cytokines were elevated in ALI patients. The concentrations of cytokines including IL-1β (A) , IL-6 (B) , IL-15 (C) , TNF-α (D) , IL-4 (E) , and IL-13 (F) were measured using ELISA kits in serum samples obtained from 24 NSCLC patients (Control) under T0 stage and 24 ALI patients. *** P < 0.001.

Article Snippet: Blood samples were immediately centrifuged at 800 × g for 10 min to obtain serum, which was applied to measure the levels of cytokines including IL-1β (#ab214025), IL-4 (#ab215089), IL-6 (#ab100573), IL-13 (#ab46038), IL-15 (#ab100554), and TNF-α (ab181421) using ELISA kits purchased from Abcam (Cambridge, MA, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: Sirt6 attenuates chondrocyte senescence and osteoarthritis progression

doi: 10.1038/s41467-022-35424-w

Figure Lengend Snippet: a Representative radiographic images and gross appearance of the cartilage of OA patients and controls, H&E, Safranin O, and Alcian blue staining. n = 6 independent biological replicates per group. b Representative images of western blot gel and quantitative analysis. n = 3 independent biological replicates per group. c Compared with controls ( n = 60), Sirt6 level was downregulated in cartilage tissues of OA patients ( n = 90). In chondrocytes of OA patients, decreased level of Sirt6 was also observed ( n = 6 independent biological replicates per group). d Immunofluorescence analysis showing the expression of Sirt6 in the cartilage tissues from OA patients and controls. n = 6 independent biological replicates per group. e Significant correlations between Sirt6 and a modified Mankin grade and synovitis grade were observed. The association between Sirt6 and OA severity was also validated by western blot ( n = 25 independent biological replicates per group). f – j Representative images showing the p16 INK4a and TNF-α expression levels ( f ), DNA damage ( g ), mitochondrial membrane potential ( h ), SA-β-Gal positivity ( i ), and ROS level ( j ) in human OA chondrocytes that were transfected by pcDNA3.1-Sirt6, Sirt6 siRNA or their corresponding controls and quantification. n = 6 independent biological replicates per group. k Immunohistochemistry staining of representative images of indicated markers in articular cartilage of DMM-induced mice treated by Ad-Sirt6, Ad-Sirt6 shRNA, or control Ad. n = 6 mice per group. l Safranin O/fast green and H&E staining of knee joints of DMM-induced mice receiving different treatments. n = 6 mice per group. Scar bar: a 200 μm, l 50 μm, d , f , h – k 20 μm, g 5 μm. Data were presented as the mean ± s.e.m ( b , right panel of c , and f – k ) or median (25–75th percentiles) (left panel of c , l ). P values are from two-tailed Mann–Whitney U -test (left panel of c ), two-tailed unpaired t -test ( b , right panel of c , d ), or two-tailed Spearman’s correlation test (left panel of e ), one-way ANOVA test followed by Tukey’s post hoc test (TNF-α level in f, i and p16 INK4a level in k ), Brown–Forsythe and Welch ANOVA test followed by Tamhane’s T2 post hoc analysis ( h , j , p16 INK4a level in f , TNF-α and IL-6 levels in k ) or Kruskal–Wallis test followed by Dunn’s post hoc test ( l ). Source data are provided as a Source Data file.

Article Snippet: The slides were incubated with primary antibodies, p16 INK4a (1:1000; Abcam, ab241543), TNF-α (1:500; Abcam, ab220210), IL-6 (1:100; Abcam, ab290735), Col II (1:200; Abcam, ab34712), ACAN (1:500; Abcam, ab186414), Col X (1:1000; Abcam, ab49945), p21 (1:50, Cell Signaling Technology, #2947), p53 (1:100, Cell Signaling Technology, #48818), IL-1β (1:200, Cell Signaling Technology, #12242) and HMBG1 (1:400; Abcam, ab79823), biotinylated secondary antibodies, enzyme-conjugated substrate and developed with diaminobenzidine (DAB) chromogen.

Techniques: Staining, Western Blot, Immunofluorescence, Expressing, Modification, Transfection, Immunohistochemistry, shRNA, Two Tailed Test, MANN-WHITNEY

Journal: Nature Communications

Article Title: Sirt6 attenuates chondrocyte senescence and osteoarthritis progression

doi: 10.1038/s41467-022-35424-w

Figure Lengend Snippet: a – c Representative images of immunofluorescence of p16 INK4a , p21, p53, HMBG1, IL-1β, and SA-β-Gal staining in cultured chondrocytes from 6-month-old mice (Sirt6 flox/flox and Sirt6 cKO) ( a ), 12-month-old mice (Sirt6 flox/flox and Sirt6 cKO) ( b ), and 18-month-old mice cartilage tissues (Sirt6 flox/flox and Sirt6 cKO) ( c ), respectively. n = 6 mice per group. d Representative images of Safranin O staining of cartilage tissues (medial femoral condyle) from 6-month-old mice (Sirt6 flox/flox and Sirt6 cKO), 12-month-old mice (Sirt6 flox/flox and Sirt6 cKO), and 18-month-old mice (Sirt6 flox/flox and Sirt6 cKO) and OARSI. n = 6 mice per group. e Synovial inflammation of 6-month-old mice (Sirt6 flox/flox and Sirt6 cKO), 12-month-old mice (Sirt6 flox/flox and Sirt6 cKO), and 18-month-old mice (Sirt6 flox/flox and Sirt6 cKO). n = 6 mice per group. f Representative images of Safranin O/fast green staining in cartilage tissues (medial femoral condyle) from the indicated groups (WT, Sirt6 flox/flox , and Sirt6 cKO mice undergoing sham or DMM surgery) and OARSI. n = 6 mice per group. g Representative images of immunohistochemistry of p16 INK4a , IL-6, p21, p53, IL-β, TNF-α, and HMGB1 in cartilage tissues (medial femoral condyle) from the indicated groups (WT, Sirt6 flox/flox and Sirt6 cKO mice subjected to DMM surgery) at 8 weeks post-surgery. n = 6 mice per group. h Chondrocytes apoptosis was assayed by flow cytometry in the indicated groups (WT, Sirt6 flox/flox , and Sirt6 cKO mice subjected to DMM surgery). n = 6 independent biological replicates per group. Scar bar: d – f 50 μm, a – c , g 20 μm. Data were presented as the mean ± s.e.m ( a – c , g , h ) or median (25–75th percentiles) ( d – f ). P values are from two-tailed unpaired Student’s t -test ( c , p16 INK4a , p53, HMBG1, IL-1β, and SA-β-Gal levels in a , p16 INK4a , p21, p53, HMBG1, and IL-1β levels in b ), two-tailed unpaired t -test with Welch’s correction (p21 level in a , SA-β-Gal level in b ), two-tailed Mann–Whitney U -test ( d – f ) or one-way ANOVA test followed by Tukey’s post hoc test ( g , h ). Source data are provided as a Source Data file.

Article Snippet: The slides were incubated with primary antibodies, p16 INK4a (1:1000; Abcam, ab241543), TNF-α (1:500; Abcam, ab220210), IL-6 (1:100; Abcam, ab290735), Col II (1:200; Abcam, ab34712), ACAN (1:500; Abcam, ab186414), Col X (1:1000; Abcam, ab49945), p21 (1:50, Cell Signaling Technology, #2947), p53 (1:100, Cell Signaling Technology, #48818), IL-1β (1:200, Cell Signaling Technology, #12242) and HMBG1 (1:400; Abcam, ab79823), biotinylated secondary antibodies, enzyme-conjugated substrate and developed with diaminobenzidine (DAB) chromogen.

Techniques: Immunofluorescence, Staining, Cell Culture, Immunohistochemistry, Flow Cytometry, Two Tailed Test, MANN-WHITNEY