Journal: Pharmaceuticals

Article Title: Identification of Novel Cyanopyridones and Pyrido[2,3- d ]pyrimidines as Anticancer Agents with Dual VEGFR-2/HER-2 Inhibitory Action: Synthesis, Biological Evaluation and Molecular Docking Studies

doi: 10.3390/ph15101262

Figure Lengend Snippet: Effects of compounds 5e and 5a on VEGFR-2 (upper) and HER-2 (lower) in MCF-7 cells compared to control. Data are represented as means ± S.D.

Article Snippet: Assessment was performed using a VEGFR-2 (human) SimpleStep ELISA kit (ab213476) and HER-2 (human) SimpleStep ELISA kit (ab100510) (abcam ® Cambridge, CB2 0AX, Cambridge, UK) according to manufacturer instructions.

Techniques:

Journal: Pharmaceuticals

Article Title: Identification of Novel Cyanopyridones and Pyrido[2,3- d ]pyrimidines as Anticancer Agents with Dual VEGFR-2/HER-2 Inhibitory Action: Synthesis, Biological Evaluation and Molecular Docking Studies

doi: 10.3390/ph15101262

Figure Lengend Snippet: Superimposition of the crystal reference ( cyan ) and the docking pose ( rose ) for sorafenib with the VEGFR-2 ( A ) and TAK-285 in the HER-2 ( B ) binding sites.

Article Snippet: Assessment was performed using a VEGFR-2 (human) SimpleStep ELISA kit (ab213476) and HER-2 (human) SimpleStep ELISA kit (ab100510) (abcam ® Cambridge, CB2 0AX, Cambridge, UK) according to manufacturer instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Identification of Novel Cyanopyridones and Pyrido[2,3- d ]pyrimidines as Anticancer Agents with Dual VEGFR-2/HER-2 Inhibitory Action: Synthesis, Biological Evaluation and Molecular Docking Studies

doi: 10.3390/ph15101262

Figure Lengend Snippet: Two-dimensional (2D) interaction diagram for the binding of TAK-285 with the active site of HER-2 (PDB: 3RCD).

Article Snippet: Assessment was performed using a VEGFR-2 (human) SimpleStep ELISA kit (ab213476) and HER-2 (human) SimpleStep ELISA kit (ab100510) (abcam ® Cambridge, CB2 0AX, Cambridge, UK) according to manufacturer instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Identification of Novel Cyanopyridones and Pyrido[2,3- d ]pyrimidines as Anticancer Agents with Dual VEGFR-2/HER-2 Inhibitory Action: Synthesis, Biological Evaluation and Molecular Docking Studies

doi: 10.3390/ph15101262

Figure Lengend Snippet: Two-dimensional (2D) diagram for the binding interactions of compound 5a ( A ) and compound 5e ( B ) in the HER-2 active site (PDB: 3RCD).

Article Snippet: Assessment was performed using a VEGFR-2 (human) SimpleStep ELISA kit (ab213476) and HER-2 (human) SimpleStep ELISA kit (ab100510) (abcam ® Cambridge, CB2 0AX, Cambridge, UK) according to manufacturer instructions.

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Identification of Novel Cyanopyridones and Pyrido[2,3- d ]pyrimidines as Anticancer Agents with Dual VEGFR-2/HER-2 Inhibitory Action: Synthesis, Biological Evaluation and Molecular Docking Studies

doi: 10.3390/ph15101262

Figure Lengend Snippet: The RMSF analysis for the MDSs: HER-2 ( A ) and VEGFR-2 ( B ).

Article Snippet: Assessment was performed using a VEGFR-2 (human) SimpleStep ELISA kit (ab213476) and HER-2 (human) SimpleStep ELISA kit (ab100510) (abcam ® Cambridge, CB2 0AX, Cambridge, UK) according to manufacturer instructions.

Techniques:

Journal: Pharmaceuticals

Article Title: Identification of Novel Cyanopyridones and Pyrido[2,3- d ]pyrimidines as Anticancer Agents with Dual VEGFR-2/HER-2 Inhibitory Action: Synthesis, Biological Evaluation and Molecular Docking Studies

doi: 10.3390/ph15101262

Figure Lengend Snippet: The per-residue contributions for the binding free energy of 5e with HER-2 ( A ) and VEGFR-2 ( B ).

Article Snippet: Assessment was performed using a VEGFR-2 (human) SimpleStep ELISA kit (ab213476) and HER-2 (human) SimpleStep ELISA kit (ab100510) (abcam ® Cambridge, CB2 0AX, Cambridge, UK) according to manufacturer instructions.

Techniques: Binding Assay

Journal: Bioengineered

Article Title: Overexpression of PLK1 relieved the myocardial ischemia-reperfusion injury of rats through inducing the mitophagy and regulating the p-AMPK/FUNDC1 axis.

doi: 10.1080/21655979.2021.1938500

Figure Lengend Snippet: Figure 2. Overexpression of PLK1 relieved the ischemia reperfusion induced myocardial infarction of rats. (a) Western blot analysis of the expression of PLK1 in myocardium of rats determined by. (b) the myocardial infarction area detected by evans blue staining. (c) the levels of LDH in the serum of rats determined by ELISA. (d) Western blot analysis of the expression of PLK1 in H9c2 cells. (e) the survival rates of H9c2 cells detected by CCK-8 assay. (f) the levels of GSH, GPX and SOD in H9c2 cells detected by ELISA. ## p < 0.01 IR+Ad-PLK1 vs IR+Ad-NC, **p < 0.01 sham+Ad-NC vs IR+Ad-NC.

Article Snippet: Rat LDH ELISA kit (Abcam, ab183367), human GSH ELISA kit (Abcam, ab19534), human GPX ELISA kit (Abcam, ab195174) and human SOD ELISA kit (Abcam, ab178012) were used for the detection of the expression of these factors in serum of rats or culture medium of H9c2 cells.

Techniques: Over Expression, Western Blot, Expressing, Staining, Enzyme-linked Immunosorbent Assay, CCK-8 Assay

Journal: Bioengineered

Article Title: Overexpression of PLK1 relieved the myocardial ischemia-reperfusion injury of rats through inducing the mitophagy and regulating the p-AMPK/FUNDC1 axis.

doi: 10.1080/21655979.2021.1938500

Figure Lengend Snippet: Figure 4. Overexpression of PLK1 induced the mitophagy of myocardium of rats suffered from the ischemia reperfusion. (a, b) Western blot analysis of the expression of mitophagy related proteins in myocardium of rats and H9c2 cells. (c) Immunofluorescence analysis of the location of mitochondrion and lysosome in H9c2 cells. (d) The production of ATP in H9c2 cells determined by ELISA. ##p < 0.01 IR+Ad-PLK1 vs IR+Ad-NC, **p < 0.01 sham+Ad-NC vs IR+Ad-NC.

Article Snippet: Rat LDH ELISA kit (Abcam, ab183367), human GSH ELISA kit (Abcam, ab19534), human GPX ELISA kit (Abcam, ab195174) and human SOD ELISA kit (Abcam, ab178012) were used for the detection of the expression of these factors in serum of rats or culture medium of H9c2 cells.

Techniques: Over Expression, Western Blot, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay