anti angiotensin ii receptor type 2 antibody  (Alomone Labs)

Journal: Neural Regeneration Research

Article Title: Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease

doi: 10.4103/NRR.NRR-D-23-01542

Figure Lengend Snippet: Primary and secondary antibodies used in this study

Article Snippet: P2Y12R , Rabbit, polyclonal , 1:1000 WB , Alomone Labs, Jerusalem, Israel , AAR-012 , AB_2040074.

Techniques: Western Blot, Immunohistochemistry

Journal: PLoS ONE

Article Title: Commercially Available Angiotensin II At 2 Receptor Antibodies Are Nonspecific

doi: 10.1371/journal.pone.0069234

Figure Lengend Snippet: (A) and (B) Antibody 2818-1 from Epitomics (dilution 1:400). The AT 2 receptor antibody reacts with parenchymal microvessels (indicated with arrowheads in A and B, and in box in panel A) in the cerebral motor cortex from -1.22 mm to Bregma, and the immunocytochemistry is similar in wild-type (WT) (A) and AT 2 knock-out (KO) mice (B). (C) and (D) Antibody sc-9040 from Santa Cruz (dilution 1:2000). The antibody detects ciliated ependymal cells (arrowheads) of the lateral ventricle (V), and the staining is similar in the wild type (C) or knockout mice (D) located at the same coordinates as A and B. (E) and (F): antibody AAR-012 from Alomone (dilution 1:3000). In the cerebral motor cortex, the antibody detects cell colocalization with the neuronal marker NeuN (indicated with arrowheads in E and F and in the boxes in panel E). The staining is similar in wild-type (E) and AT 2 receptor knockout (F) mice. Scale bar = 20 µm.

Article Snippet: One of the antibodies tested (AAR-012 from Alomone lab) was raised against amino acids 21-35, untranslated in AT 2 knockout mice.

Techniques: Immunocytochemistry, Knock-Out, Staining, Marker