a594 dnaase i  (Thermo Fisher)


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    Structured Review

    Thermo Fisher a594 dnaase i
    A594 Dnaase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a594 dnaase i/product/Thermo Fisher
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a594 dnaase i - by Bioz Stars, 2020-03
    81/100 stars

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    Related Articles

    Immunocytochemistry:

    Article Title: Netrin-1 Promotes Excitatory Synaptogenesis between Cortical Neurons by Initiating Synapse Assembly
    Article Snippet: Paragraph title: Immunocytochemistry. ... To enhance the visualization of F-actin, cells were fixed in cytoskeleton buffer (10 m m MES, 3 m m MgCl2 , 138 m m KCl, 2 m m EGTA, 0.32 m sucrose, 4% PFA, pH 6) for 15 min at RT, washed in PBS, permeabilized in 100% acetone (−20°C) for 5 min, washed in PBS, and incubated with A488 phalloidin and A594 DNAase I (Invitrogen) in PBS with 0.1% Triton X-100.

    Immunolabeling:

    Article Title: Netrin-1 Promotes Excitatory Synaptogenesis between Cortical Neurons by Initiating Synapse Assembly
    Article Snippet: Antibodies were diluted in blocking solution (1% milk or BSA, 0.1% Triton X-100 in 0.1 m PBS, pH 7.4); incubated overnight; and immunolabeling visualized with Alexa Fluor 488-, Alexa Fluor 546-, or Alexa Fluor 633-coupled secondary antibodies (Invitrogen). .. To enhance the visualization of F-actin, cells were fixed in cytoskeleton buffer (10 m m MES, 3 m m MgCl2 , 138 m m KCl, 2 m m EGTA, 0.32 m sucrose, 4% PFA, pH 6) for 15 min at RT, washed in PBS, permeabilized in 100% acetone (−20°C) for 5 min, washed in PBS, and incubated with A488 phalloidin and A594 DNAase I (Invitrogen) in PBS with 0.1% Triton X-100.

    Blocking Assay:

    Article Title: Netrin-1 Promotes Excitatory Synaptogenesis between Cortical Neurons by Initiating Synapse Assembly
    Article Snippet: Antibodies were diluted in blocking solution (1% milk or BSA, 0.1% Triton X-100 in 0.1 m PBS, pH 7.4); incubated overnight; and immunolabeling visualized with Alexa Fluor 488-, Alexa Fluor 546-, or Alexa Fluor 633-coupled secondary antibodies (Invitrogen). .. To enhance the visualization of F-actin, cells were fixed in cytoskeleton buffer (10 m m MES, 3 m m MgCl2 , 138 m m KCl, 2 m m EGTA, 0.32 m sucrose, 4% PFA, pH 6) for 15 min at RT, washed in PBS, permeabilized in 100% acetone (−20°C) for 5 min, washed in PBS, and incubated with A488 phalloidin and A594 DNAase I (Invitrogen) in PBS with 0.1% Triton X-100.

    Incubation:

    Article Title: Netrin-1 Promotes Excitatory Synaptogenesis between Cortical Neurons by Initiating Synapse Assembly
    Article Snippet: .. To enhance the visualization of F-actin, cells were fixed in cytoskeleton buffer (10 m m MES, 3 m m MgCl2 , 138 m m KCl, 2 m m EGTA, 0.32 m sucrose, 4% PFA, pH 6) for 15 min at RT, washed in PBS, permeabilized in 100% acetone (−20°C) for 5 min, washed in PBS, and incubated with A488 phalloidin and A594 DNAase I (Invitrogen) in PBS with 0.1% Triton X-100. .. To visualize the synthesis of new protein, dfb, IgG, or rapamycin were added to cells in culture with methionine-free media and Click-it AHA reagent, following the protocol provided by the manufacturer (Sigma-Aldrich), 15 min before addition of beads.

    Imaging:

    Article Title: Netrin-1 Promotes Excitatory Synaptogenesis between Cortical Neurons by Initiating Synapse Assembly
    Article Snippet: For imaging F-actin in conjunction with antibody staining, phalloidin A647 (Invitrogen) was incubated with secondary antibodies. .. To enhance the visualization of F-actin, cells were fixed in cytoskeleton buffer (10 m m MES, 3 m m MgCl2 , 138 m m KCl, 2 m m EGTA, 0.32 m sucrose, 4% PFA, pH 6) for 15 min at RT, washed in PBS, permeabilized in 100% acetone (−20°C) for 5 min, washed in PBS, and incubated with A488 phalloidin and A594 DNAase I (Invitrogen) in PBS with 0.1% Triton X-100.

    Staining:

    Article Title: Netrin-1 Promotes Excitatory Synaptogenesis between Cortical Neurons by Initiating Synapse Assembly
    Article Snippet: For imaging F-actin in conjunction with antibody staining, phalloidin A647 (Invitrogen) was incubated with secondary antibodies. .. To enhance the visualization of F-actin, cells were fixed in cytoskeleton buffer (10 m m MES, 3 m m MgCl2 , 138 m m KCl, 2 m m EGTA, 0.32 m sucrose, 4% PFA, pH 6) for 15 min at RT, washed in PBS, permeabilized in 100% acetone (−20°C) for 5 min, washed in PBS, and incubated with A488 phalloidin and A594 DNAase I (Invitrogen) in PBS with 0.1% Triton X-100.

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    Thermo Fisher deoxyribonuclease i
    Deoxyribonuclease I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyribonuclease i/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    deoxyribonuclease i - by Bioz Stars, 2020-03
    99/100 stars
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