Journal: MethodsX

Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method ☆

doi: 10.1016/j.mex.2026.103871

Figure Lengend Snippet: Final purified mitochondrial fractions stained with BioTracker 633 Red Mitochondria Dye (middle) and TOM20 (left) show viable pure mitochondria (merged; right). Scale bar is 5 µm.

Article Snippet: Mitochondria Extraction Kit – Tissue (Miltenyi Biotec, Cat#130-097-340) Mitochondria Isolation Kit, mouse tissue (Miltenyi Biotec, Cat#130-096-946) Myelin Removal Beads II, human, mouse, rat (Miltenyi Biotec, Cat#130-096-433) Dulbecco’s PBS (1X), without Ca and Mg, without Phenol Red (Capricorn Scientific, Cat#PBS-1A) Roche cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail (Roche, Cat#11836170001) Bovine Serum Albumin heat shock fraction, pH 7, ≥ 98 % (Sigma-Aldrich, Cat#A7906) BioTracker 633 Red Mitochondria Dye (Sigma-Aldrich, Cat#SCT137) SuperSignalTM West Femto Maximum Sensitivity Substrate (Thermo Scientific, Cat#34096) PierceTM BCA Protein Assay Kit (Thermo Scientific, Cat#23227)

Techniques: Purification, Staining

Journal: Biochemistry and Biophysics Reports

Article Title: Distinct hyaluronan content and hierarchical organization of collagen-elastic fiber assemblies in subcutaneous fascia

doi: 10.1016/j.bbrep.2026.102536

Figure Lengend Snippet: Observation of fibrillin fibers and elastin fibers in the subcutaneous fascia samples. For Loose fascia (A) and dense fascia (B), biochemically treated samples were prepared as in . These were subjected to fibrillin staining with Calcein (green) and elastin fluorescence staining with a specific antibody (red). Fluorescently stained samples were spread as uniformly as possible on glass slides for observation. Phase-contrast images (a, e, i, m), merged images of fibrillin (green) and elastin (red) fluorescence (b, f, j, n), fibrillin-only fluorescence images (c, g, k, o), and elastin-only fluorescence images (d, h, l, p) are shown. Biochemical treatments are as follows: Control, untreated sample; U, urea-treated sample; G, guanidine-HCl-treated sample; E2h, elastase-treated (2 h) sample; E2d, elastase-treated (2 days) sample. Scale bars are 100 μm.

Article Snippet: As calcein (Dojindo Molecular Technologies Inc., Kumamoto, Japan) selectively binds to fibrillin-rich elastic fibers in a calcium-dependent manner [ ], this method accurately visualizes the elastic fiber distribution.

Techniques: Staining, Fluorescence, Control