a protinin  (Millipore)

 
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    Name:
    Aprotinin
    Description:
    Trypsin inhibitor pancreas type from bovine lung It is known as Pancreatic trypsin inhibitor BPTI Aprotinin also known as pancreatic trypsin inhibitor and trypsin kallikrein inhibitor is found to be expressed in lungs spleen liver and pancreas It is also found to be present in the free form in calf serum
    Catalog Number:
    roapro
    Price:
    None
    Applications:
    Aprotinin is used for the protection of proteins and enzymes during isolation/purification. The inhibition of protease activity increases the lifetime of cells in cell and tissue culture studies.. Further applications: Purification of urokinase, trypsin, and chymotrypsin on immobilized aprotinin. Quantification of kallikrein activity in mixtures of esterases and proteases. Controlled degradation of substrates by avoiding nonspecific proteolysis in clinical chemical tests. Aprotinin as a model protein in protein-folding studies. Molecular weight marker in SDS-polyacrylamide gel electrophoresis
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    Structured Review

    Millipore a protinin
    Trypsin inhibitor pancreas type from bovine lung It is known as Pancreatic trypsin inhibitor BPTI Aprotinin also known as pancreatic trypsin inhibitor and trypsin kallikrein inhibitor is found to be expressed in lungs spleen liver and pancreas It is also found to be present in the free form in calf serum
    https://www.bioz.com/result/a protinin/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a protinin - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Virulence Associated Gene 8 of Bordetella pertussis Enhances Contact System Activity by Inhibiting the Regulatory Function of Complement Regulator C1 Inhibitor
    Article Snippet: All antibodies were diluted in 1% skimmed milk in PBS-T. For detection, the Pierce ECL Western Blotting Substrate (Thermofisher Scientific, Waltham, MA, USA) was used and visualized using the ImageQuant (GE Life Sciences, Chicago, IL, USA). .. For assessment of HK cleavage upon incubation of plasma with B. pertussis , several bacterial strains (2 × 109 CFU) were incubated with 50% plasma either alone or in the presence of the contact protease inhibitors aprotinin ( ) (100 units/mL, Sigma), which inhibits PK and d -phenylalanyl-prolyl-arginyl chloromethyl ketone ( ) (PPACK; 200 µM, Hematologic Technologies, Essex Junction, VT, USA), a multi-target serine protease inhibitor which restricts auto activation and self-digestion of FXIIa, and sampled after 30 min at 37°C shaking at 300 rpm. .. Plasma samples incubated with βFXIIa (16.7 nM corresponding to 0.5 µg/mL) or only buffer A were taken along as positive and negative controls, respectively.

    Article Title: Virulence Associated Gene 8 of Bordetella pertussis Enhances Contact System Activity by Inhibiting the Regulatory Function of Complement Regulator C1 Inhibitor
    Article Snippet: All antibodies were diluted in 1% skimmed milk in PBS-T. For detection, the Pierce ECL Western Blotting Substrate (Thermofisher Scientific, Waltham, MA, USA) was used and visualized using the ImageQuant (GE Life Sciences, Chicago, IL, USA). .. For assessment of HK cleavage upon incubation of plasma with B. pertussis , several bacterial strains (2 × 109 CFU) were incubated with 50% plasma either alone or in the presence of the contact protease inhibitors aprotinin ( ) (100 units/mL, Sigma), which inhibits PK and d -phenylalanyl-prolyl-arginyl chloromethyl ketone ( ) (PPACK; 200 µM, Hematologic Technologies, Essex Junction, VT, USA), a multi-target serine protease inhibitor which restricts auto activation and self-digestion of FXIIa, and sampled after 30 min at 37°C shaking at 300 rpm. .. Plasma samples incubated with βFXIIa (16.7 nM corresponding to 0.5 µg/mL) or only buffer A were taken along as positive and negative controls, respectively.

    Protease Inhibitor:

    Article Title: Virulence Associated Gene 8 of Bordetella pertussis Enhances Contact System Activity by Inhibiting the Regulatory Function of Complement Regulator C1 Inhibitor
    Article Snippet: All antibodies were diluted in 1% skimmed milk in PBS-T. For detection, the Pierce ECL Western Blotting Substrate (Thermofisher Scientific, Waltham, MA, USA) was used and visualized using the ImageQuant (GE Life Sciences, Chicago, IL, USA). .. For assessment of HK cleavage upon incubation of plasma with B. pertussis , several bacterial strains (2 × 109 CFU) were incubated with 50% plasma either alone or in the presence of the contact protease inhibitors aprotinin ( ) (100 units/mL, Sigma), which inhibits PK and d -phenylalanyl-prolyl-arginyl chloromethyl ketone ( ) (PPACK; 200 µM, Hematologic Technologies, Essex Junction, VT, USA), a multi-target serine protease inhibitor which restricts auto activation and self-digestion of FXIIa, and sampled after 30 min at 37°C shaking at 300 rpm. .. Plasma samples incubated with βFXIIa (16.7 nM corresponding to 0.5 µg/mL) or only buffer A were taken along as positive and negative controls, respectively.

    Article Title: Virulence Associated Gene 8 of Bordetella pertussis Enhances Contact System Activity by Inhibiting the Regulatory Function of Complement Regulator C1 Inhibitor
    Article Snippet: All antibodies were diluted in 1% skimmed milk in PBS-T. For detection, the Pierce ECL Western Blotting Substrate (Thermofisher Scientific, Waltham, MA, USA) was used and visualized using the ImageQuant (GE Life Sciences, Chicago, IL, USA). .. For assessment of HK cleavage upon incubation of plasma with B. pertussis , several bacterial strains (2 × 109 CFU) were incubated with 50% plasma either alone or in the presence of the contact protease inhibitors aprotinin ( ) (100 units/mL, Sigma), which inhibits PK and d -phenylalanyl-prolyl-arginyl chloromethyl ketone ( ) (PPACK; 200 µM, Hematologic Technologies, Essex Junction, VT, USA), a multi-target serine protease inhibitor which restricts auto activation and self-digestion of FXIIa, and sampled after 30 min at 37°C shaking at 300 rpm. .. Plasma samples incubated with βFXIIa (16.7 nM corresponding to 0.5 µg/mL) or only buffer A were taken along as positive and negative controls, respectively.

    Activation Assay:

    Article Title: Virulence Associated Gene 8 of Bordetella pertussis Enhances Contact System Activity by Inhibiting the Regulatory Function of Complement Regulator C1 Inhibitor
    Article Snippet: All antibodies were diluted in 1% skimmed milk in PBS-T. For detection, the Pierce ECL Western Blotting Substrate (Thermofisher Scientific, Waltham, MA, USA) was used and visualized using the ImageQuant (GE Life Sciences, Chicago, IL, USA). .. For assessment of HK cleavage upon incubation of plasma with B. pertussis , several bacterial strains (2 × 109 CFU) were incubated with 50% plasma either alone or in the presence of the contact protease inhibitors aprotinin ( ) (100 units/mL, Sigma), which inhibits PK and d -phenylalanyl-prolyl-arginyl chloromethyl ketone ( ) (PPACK; 200 µM, Hematologic Technologies, Essex Junction, VT, USA), a multi-target serine protease inhibitor which restricts auto activation and self-digestion of FXIIa, and sampled after 30 min at 37°C shaking at 300 rpm. .. Plasma samples incubated with βFXIIa (16.7 nM corresponding to 0.5 µg/mL) or only buffer A were taken along as positive and negative controls, respectively.

    Article Title: Virulence Associated Gene 8 of Bordetella pertussis Enhances Contact System Activity by Inhibiting the Regulatory Function of Complement Regulator C1 Inhibitor
    Article Snippet: All antibodies were diluted in 1% skimmed milk in PBS-T. For detection, the Pierce ECL Western Blotting Substrate (Thermofisher Scientific, Waltham, MA, USA) was used and visualized using the ImageQuant (GE Life Sciences, Chicago, IL, USA). .. For assessment of HK cleavage upon incubation of plasma with B. pertussis , several bacterial strains (2 × 109 CFU) were incubated with 50% plasma either alone or in the presence of the contact protease inhibitors aprotinin ( ) (100 units/mL, Sigma), which inhibits PK and d -phenylalanyl-prolyl-arginyl chloromethyl ketone ( ) (PPACK; 200 µM, Hematologic Technologies, Essex Junction, VT, USA), a multi-target serine protease inhibitor which restricts auto activation and self-digestion of FXIIa, and sampled after 30 min at 37°C shaking at 300 rpm. .. Plasma samples incubated with βFXIIa (16.7 nM corresponding to 0.5 µg/mL) or only buffer A were taken along as positive and negative controls, respectively.

    other:

    Article Title: INSULIN INDUCED EPIDERMAL GROWTH FACTOR ACTIVATION IN VASCULAR SMOOTH MUSCLE CELLS IS ADAM-DEPENDENT
    Article Snippet: Insulin, EGF, EACA, and aprotinin were purchased from Sigma Chemical Co (St. Louis, MO).

    Article Title: Temporal and Pharmacological Characterization of Angiostatin Release and Generation by Human Platelets: Implications for Endothelial Cell Migration
    Article Snippet: Prostacyclin-sodium salt, S-nitroso-glutathione, acetylsalicyclic acid, MRS2395, RGDS peptide, and aprotinin were obtained from Sigma (Mississauga, ONT, Canada).

    Inhibition:

    Article Title: Common and specific effects of TIE2 mutations causing venous malformations
    Article Snippet: .. In serine protease inhibition assays both the gel and media were supplemented with 25 µg/ml aprotinin (Sigma-Aldrich). .. After 16 h cells were fixed with 4% paraformaldehyde and imaged for quantification using a phase-contrast microscope.

    Construct:

    Article Title: Compaction Properties of an Intrinsically Disordered Protein: Sic1 and Its Kinase-Inhibitor Domain
    Article Snippet: Chromatography was carried out at room temperature in 50 mM sodium phosphate, pH 8.0, 200 mM NaCl at a flow rate of 0.5 mL/min, and monitored by absorbance at 280 nm. .. A calibration curve was constructed using the following standards (0.75 mg/mL): bovine aprotinin (6,500 Da), horse cytochrome c (12,400 Da), horse myoglobin (17,600 Da), chicken ovalbumin (45,000 Da), equine apoferritin (80,000 Da) (Sigma Aldrich, St. Louis, MO), and recombinant green fluorescent protein (29,800 Da). .. A second calibration curve was constructed to calculate the Rh values of Sic1FL and Sic1Δ214 , by plotting the Rh values of the standards (ovalbumin, myoglobin, cytochrome c , and aprotinin) against their relatve elution volume ( ).

    Recombinant:

    Article Title: Compaction Properties of an Intrinsically Disordered Protein: Sic1 and Its Kinase-Inhibitor Domain
    Article Snippet: Chromatography was carried out at room temperature in 50 mM sodium phosphate, pH 8.0, 200 mM NaCl at a flow rate of 0.5 mL/min, and monitored by absorbance at 280 nm. .. A calibration curve was constructed using the following standards (0.75 mg/mL): bovine aprotinin (6,500 Da), horse cytochrome c (12,400 Da), horse myoglobin (17,600 Da), chicken ovalbumin (45,000 Da), equine apoferritin (80,000 Da) (Sigma Aldrich, St. Louis, MO), and recombinant green fluorescent protein (29,800 Da). .. A second calibration curve was constructed to calculate the Rh values of Sic1FL and Sic1Δ214 , by plotting the Rh values of the standards (ovalbumin, myoglobin, cytochrome c , and aprotinin) against their relatve elution volume ( ).

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  • About
  • News
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  • 99
    Millipore a protinin
    A Protinin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a protinin/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a protinin - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    97
    Millipore protinin
    Protinin, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protinin/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protinin - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

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