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Kinexus Bioinformatics Corporation a pleuropneumoniae infection
Antibody microarray summary
A Pleuropneumoniae Infection, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Current applications of antibody microarrays"

Article Title: Current applications of antibody microarrays

Journal: Clinical Proteomics

doi: 10.1186/s12014-018-9184-2

Antibody microarray summary
Figure Legend Snippet: Antibody microarray summary

Techniques Used: Microarray, Infection, Biomarker Assay



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Image Search Results


Actinobacillus  pleuropneumoniae  reference strains used for the development of the high resolution melting (HRM) assays

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Actinobacillus pleuropneumoniae reference strains used for the development of the high resolution melting (HRM) assays

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques:

Clinical isolates of Actinobacillus  pleuropneumoniae  used in the study

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Clinical isolates of Actinobacillus pleuropneumoniae used in the study

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: Multiplex Assay

APP‐HRM 1 primers for detection of Actinobacillus  pleuropneumoniae  and biovar 2

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: APP‐HRM 1 primers for detection of Actinobacillus pleuropneumoniae and biovar 2

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: Sequencing, Amplification, Concentration Assay

APP‐HRM2 primers for detection of Actinobacillus  pleuropneumoniae  serovars 1, 2, 4, 5, 7, 8, 10, 13, and 15

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: APP‐HRM2 primers for detection of Actinobacillus pleuropneumoniae serovars 1, 2, 4, 5, 7, 8, 10, 13, and 15

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: Sequencing, Amplification, Concentration Assay

APP‐HRM3 primers for detection of Actinobacillus  pleuropneumoniae  serovars 3, 6, 9, 11, 12, 14, 16, 17, 18, and 19

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: APP‐HRM3 primers for detection of Actinobacillus pleuropneumoniae serovars 3, 6, 9, 11, 12, 14, 16, 17, 18, and 19

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: Sequencing, Amplification, Concentration Assay

High resolution melting (HRM) for identification of Actinobacillus pleuropneumoniae and biovar 2. APP‐HRM1 assay allows targeting the species‐specific gene apxIV for identification of A. pleuropneumoniae and nadV for biovar 2 detection, respectively. A. pleuropneumoniae strains N273 (serovar reference strain 13), 3906 (serovar reference strain 14), SS4935 (serovar 2), and SS4936 (serovar 2) (represented in blue) all contain apxIV and nadV , whereas all remaining A. pleuropneumoniae strains tested in the study (represented in red) are biovar 1 and therefore only harbor apxIV

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: High resolution melting (HRM) for identification of Actinobacillus pleuropneumoniae and biovar 2. APP‐HRM1 assay allows targeting the species‐specific gene apxIV for identification of A. pleuropneumoniae and nadV for biovar 2 detection, respectively. A. pleuropneumoniae strains N273 (serovar reference strain 13), 3906 (serovar reference strain 14), SS4935 (serovar 2), and SS4936 (serovar 2) (represented in blue) all contain apxIV and nadV , whereas all remaining A. pleuropneumoniae strains tested in the study (represented in red) are biovar 1 and therefore only harbor apxIV

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques:

Illustration of high resolution (HRM) assay APP‐HRM2 (a–c) and APP‐HRM3 (d–f) capable of differentiating 19 serovars of Actinobacillus pleuropneumoniae . (a) Melting curves of the HRM step applying primers from APP‐HRM2. (b) Normalized plot for APP‐HRM2. (c) Difference plot for APP‐HRM2 normalized with DNA from A. pleuropneumoniae serovar 15. (d) Melting curves of the HRM step applying primers from APP‐HRM3. (e) Normalized plot for APP‐HRM3 (f) difference plot for APP‐HRM3 normalized with DNA from A. pleuropneumoniae serovar 14

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Illustration of high resolution (HRM) assay APP‐HRM2 (a–c) and APP‐HRM3 (d–f) capable of differentiating 19 serovars of Actinobacillus pleuropneumoniae . (a) Melting curves of the HRM step applying primers from APP‐HRM2. (b) Normalized plot for APP‐HRM2. (c) Difference plot for APP‐HRM2 normalized with DNA from A. pleuropneumoniae serovar 15. (d) Melting curves of the HRM step applying primers from APP‐HRM3. (e) Normalized plot for APP‐HRM3 (f) difference plot for APP‐HRM3 normalized with DNA from A. pleuropneumoniae serovar 14

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: HRM Assay

Identification of Actinobacillus pleuropneumoniae serovar 9 and serovar 11 using high resolution melting (HRM) assay APP‐HRM3. Differentiation of A. pleuropneumoniae serovar 9 and serovar 11 is based on a single‐nucleotide polymorphism in cps9 / 11F . A 10‐fold dilution series of A. pleuropneumoniae reference strains serovar 9 (light green) and serovar 11 (dark green) was tested in triplicates. Illustration of (a) PCR amplification curves, (b) melting curves of the HRM step, (c) normalized plot, and (d) difference plot normalized with genomic DNA from A. pleuropneumoniae serovar 11 (12.5 ng) visualizing two groups corresponding to A. pleuropneumoniae serovars 9 and 11. PCR, polymerase chain reaction

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Identification of Actinobacillus pleuropneumoniae serovar 9 and serovar 11 using high resolution melting (HRM) assay APP‐HRM3. Differentiation of A. pleuropneumoniae serovar 9 and serovar 11 is based on a single‐nucleotide polymorphism in cps9 / 11F . A 10‐fold dilution series of A. pleuropneumoniae reference strains serovar 9 (light green) and serovar 11 (dark green) was tested in triplicates. Illustration of (a) PCR amplification curves, (b) melting curves of the HRM step, (c) normalized plot, and (d) difference plot normalized with genomic DNA from A. pleuropneumoniae serovar 11 (12.5 ng) visualizing two groups corresponding to A. pleuropneumoniae serovars 9 and 11. PCR, polymerase chain reaction

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: HRM Assay, Amplification, Polymerase Chain Reaction

Standard curves of 10‐fold dilution series acquired by the serovar‐specific APP‐HRM1 assay in the dynamic range of 5 × 10 6 –5 genome equivalents for all reference Actinobacillus pleuropneumoniae strains are represented. PCR efficiencies between 93% and 103% with high correlation coefficients ( R 2 > 0.99) were obtained. APP‐HRM, Actinobacillus pleuropneumoniae ‐high resolution melting; PCR, polymerase chain reaction

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Standard curves of 10‐fold dilution series acquired by the serovar‐specific APP‐HRM1 assay in the dynamic range of 5 × 10 6 –5 genome equivalents for all reference Actinobacillus pleuropneumoniae strains are represented. PCR efficiencies between 93% and 103% with high correlation coefficients ( R 2 > 0.99) were obtained. APP‐HRM, Actinobacillus pleuropneumoniae ‐high resolution melting; PCR, polymerase chain reaction

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: Polymerase Chain Reaction

Standard curves of 10‐fold dilution series acquired by the serovar‐specific APP‐HRM2 and APP‐HRM3 assays in the range of linearity for all serovar reference strains of Actinobacillus pleuropneumoniae are represented. PCR efficiencies between 90% and 108% with correlation coefficients ( R 2 > 0.96) were obtained. APP‐HRM, Actinobacillus pleuropneumoniae ‐high resolution melting; PCR, polymerase chain reaction

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Standard curves of 10‐fold dilution series acquired by the serovar‐specific APP‐HRM2 and APP‐HRM3 assays in the range of linearity for all serovar reference strains of Actinobacillus pleuropneumoniae are represented. PCR efficiencies between 90% and 108% with correlation coefficients ( R 2 > 0.96) were obtained. APP‐HRM, Actinobacillus pleuropneumoniae ‐high resolution melting; PCR, polymerase chain reaction

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: Polymerase Chain Reaction

Identification of Actinobacillus pleuropneumoniae targeting apxIV (APP‐HRM1) illustrating high sensitivity. APP‐HRM1 was performed with an A. pleuropneumoniae tenfold dilution series of genomic DNA (here as an example A. pleuropneumoniae serovar 15) using primers targeting apxIV . DNA quantities of the tenfold dilution series were 5,000,000 genome equivalents (GE) (red), 500,000 GE (orange), 50,000 GE (yellow), 5000 GE (green), 500 GE (light blue), 50 GE (blue), and 5 GE (violet). Representation of the 10‐fold dilution series as (a) qPCR amplification plot; (b) melting curves of the HRM step illustrating a limit of detection of 5 GE; (c) standard curve indicating linearity across a large range of DNA quantities between 5,000,000 GE and 5 GE with a high correlation coefficient ( R 2 > 0.99). HRM, high resolution melting; qPCR, quantitative polymerase chain reaction

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Identification of Actinobacillus pleuropneumoniae targeting apxIV (APP‐HRM1) illustrating high sensitivity. APP‐HRM1 was performed with an A. pleuropneumoniae tenfold dilution series of genomic DNA (here as an example A. pleuropneumoniae serovar 15) using primers targeting apxIV . DNA quantities of the tenfold dilution series were 5,000,000 genome equivalents (GE) (red), 500,000 GE (orange), 50,000 GE (yellow), 5000 GE (green), 500 GE (light blue), 50 GE (blue), and 5 GE (violet). Representation of the 10‐fold dilution series as (a) qPCR amplification plot; (b) melting curves of the HRM step illustrating a limit of detection of 5 GE; (c) standard curve indicating linearity across a large range of DNA quantities between 5,000,000 GE and 5 GE with a high correlation coefficient ( R 2 > 0.99). HRM, high resolution melting; qPCR, quantitative polymerase chain reaction

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: Amplification, Real-time Polymerase Chain Reaction

Efficiency and limit of detection (LOD) of APP‐HRM1, APP‐HRM2, and APP‐HRM3 targeting apxIV , nadV , and serovar‐specific cps loci

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Efficiency and limit of detection (LOD) of APP‐HRM1, APP‐HRM2, and APP‐HRM3 targeting apxIV , nadV , and serovar‐specific cps loci

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques:

Representation of high resolution melting (HRM) results of DNA samples from 15 clinical Actinobacillus pleuropneumoniae isolates collected between 2014 and 2021 in Switzerland. (a) Illustration of HRM melting curves obtained with APP‐HRM2 and APP‐HRM3. (b) For each isolate, the corresponding amplicon melting temperatures ( T m ) obtained from APP‐HRM1, APP‐HRM2, and APP‐HRM3 are shown. a Serovar determination by multiplex polymerase chain reaction (Bossé et al., ; Stringer et al., )

Journal: MicrobiologyOpen

Article Title: Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

doi: 10.1002/mbo3.1272

Figure Lengend Snippet: Representation of high resolution melting (HRM) results of DNA samples from 15 clinical Actinobacillus pleuropneumoniae isolates collected between 2014 and 2021 in Switzerland. (a) Illustration of HRM melting curves obtained with APP‐HRM2 and APP‐HRM3. (b) For each isolate, the corresponding amplicon melting temperatures ( T m ) obtained from APP‐HRM1, APP‐HRM2, and APP‐HRM3 are shown. a Serovar determination by multiplex polymerase chain reaction (Bossé et al., ; Stringer et al., )

Article Snippet: Using APP‐HRM1, A. pleuropneumoniae can be detected very sensitively between a detection limit of 5–50 GE corresponding to 125 fg–1.25 pg of DNA, representing a high sensitivity allowing to detect even low levels of A. pleuropneumoniae ‐infected tissues.

Techniques: Amplification, Multiplex Assay, Polymerase Chain Reaction

Antibody microarray summary

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Antibody microarray summary

Article Snippet: Kinexus Bioinformatics , A. pleuropneumoniae infection , KAM-850 , 854 , 26577697.

Techniques: Microarray, Infection, Biomarker Assay