plasmid miniprep kit  (Zymo Research)


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    Name:
    ZymoPURE Plasmid Miniprep Kit
    Description:
    The ZymoPURE Plasmid Miniprep Kit features a spin column based method for the purification of up to 100 µg of ultra pure endotoxin free plasmid DNA in less than 15 minutes The unique spin column design also provides zero buffer retention and a low elution volume ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA up to 3 µg µl In addition the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins salt protein and RNA The result is plasmid DNA suitable for transfection restriction endonuclease digestion bacterial transformation PCR amplification DNA sequencing and other sensitive downstream applications As an added convenience the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error free visualization and identification of complete bacterial cell lysis and neutralization
    Catalog Number:
    d4208t
    Product Aliases:
    ZymoPURE II Plasmid Miniprep Kit
    Price:
    None
    Applications:
    Plasmid DNA Purification
    Size:
    10 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research plasmid miniprep kit
    ZymoPURE Plasmid Miniprep Kit
    The ZymoPURE Plasmid Miniprep Kit features a spin column based method for the purification of up to 100 µg of ultra pure endotoxin free plasmid DNA in less than 15 minutes The unique spin column design also provides zero buffer retention and a low elution volume ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA up to 3 µg µl In addition the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins salt protein and RNA The result is plasmid DNA suitable for transfection restriction endonuclease digestion bacterial transformation PCR amplification DNA sequencing and other sensitive downstream applications As an added convenience the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error free visualization and identification of complete bacterial cell lysis and neutralization
    https://www.bioz.com/result/plasmid miniprep kit/product/Zymo Research
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    plasmid miniprep kit - by Bioz Stars, 2020-10
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Kinetics and Fidelity of Psychrophilic DNA Polymerases
    Article Snippet: .. All cloning products were first subcloned in NEB-5α strains and then purified using ZymoPURE plasmid miniprep kits (Zymo Research). .. The sequences of the expression constructs were all verified with Sanger sequencing by Sequetech.

    Agarose Gel Electrophoresis:

    Article Title: High-Throughput Screening of Coenzyme Preference Change of Thermophilic 6-Phosphogluconate Dehydrogenase from NADP+ to NAD+
    Article Snippet: .. The identified colonies embedded in the agarose gel were taken out by a sterile toothstick and then suspended in 200 μL of the P1 buffer of Zymo ZR Plasmid Miniprep™ kit followed by the Zymo ZR plasmid purification protocol. .. A tiny amount of plasmid extracted from the agarose gel was transformed into E. coli TOP10 for plasmid amplification, purification and DNA sequencing.

    Isolation:

    Article Title: The pneumococcal σX activator, ComW, is a DNA-binding protein critical for natural transformation
    Article Snippet: .. Plasmids were isolated using the ZymoPURE Plasmid Miniprep Kit (Zymo Research) and concentration was measured using a ND-1000 Spectrophotometer (Nanodrop). .. Mutations were confirmed by Sanger DNA sequencing (University of Illinois at Chicago Sequencing Core (UICSQC)).

    Quantitative RT-PCR:

    Article Title: Characterization of 17 chaperone-usher fimbriae encoded by Proteus mirabilis reveals strong conservation
    Article Snippet: .. PCR using primers targeting rpoA (RNA polymerase A) with cDNA templates with or without reverse transcriptase treatment was used to confirm lack of genomic contamination. cDNA was then purified and concentrated using a plasmid miniprep kit (Zymo) with the following modifications: PB buffer (Qiagen) was used to bind cDNA to the columns, and steps 9–11 from the Zymo plasmid miniprep kit were performed according to the manufacturer’s instructions. qRT-PCR was performed to test the expression of the 17 fimbriae in duplicate 25 µl reactions using 30 ng cDNA template, 150 nM each primer (Table S1) and 12.5 µl Maxima SYBR Green/fluorescein qPCR master mix (Thermo Scientific). .. Amplification of specific genes was performed over 40 cycles with a CFX Connect thermal cycler (Bio-Rad).

    Purification:

    Article Title: Construction of a random circular permutation library using an engineered transposon.
    Article Snippet: .. Circular permutation is an important protein engineering tool used to create sequence diversity of a protein by changing its linear order of amino acid sequence. .. Circular permutation is an important protein engineering tool used to create sequence diversity of a protein by changing its linear order of amino acid sequence.

    Article Title: High-Throughput Screening of Coenzyme Preference Change of Thermophilic 6-Phosphogluconate Dehydrogenase from NADP+ to NAD+
    Article Snippet: .. The identified colonies embedded in the agarose gel were taken out by a sterile toothstick and then suspended in 200 μL of the P1 buffer of Zymo ZR Plasmid Miniprep™ kit followed by the Zymo ZR plasmid purification protocol. .. A tiny amount of plasmid extracted from the agarose gel was transformed into E. coli TOP10 for plasmid amplification, purification and DNA sequencing.

    Article Title: Characterization of 17 chaperone-usher fimbriae encoded by Proteus mirabilis reveals strong conservation
    Article Snippet: .. PCR using primers targeting rpoA (RNA polymerase A) with cDNA templates with or without reverse transcriptase treatment was used to confirm lack of genomic contamination. cDNA was then purified and concentrated using a plasmid miniprep kit (Zymo) with the following modifications: PB buffer (Qiagen) was used to bind cDNA to the columns, and steps 9–11 from the Zymo plasmid miniprep kit were performed according to the manufacturer’s instructions. qRT-PCR was performed to test the expression of the 17 fimbriae in duplicate 25 µl reactions using 30 ng cDNA template, 150 nM each primer (Table S1) and 12.5 µl Maxima SYBR Green/fluorescein qPCR master mix (Thermo Scientific). .. Amplification of specific genes was performed over 40 cycles with a CFX Connect thermal cycler (Bio-Rad).

    Article Title: Kinetics and Fidelity of Psychrophilic DNA Polymerases
    Article Snippet: .. All cloning products were first subcloned in NEB-5α strains and then purified using ZymoPURE plasmid miniprep kits (Zymo Research). .. The sequences of the expression constructs were all verified with Sanger sequencing by Sequetech.

    Real-time Polymerase Chain Reaction:

    Article Title: Characterization of 17 chaperone-usher fimbriae encoded by Proteus mirabilis reveals strong conservation
    Article Snippet: .. PCR using primers targeting rpoA (RNA polymerase A) with cDNA templates with or without reverse transcriptase treatment was used to confirm lack of genomic contamination. cDNA was then purified and concentrated using a plasmid miniprep kit (Zymo) with the following modifications: PB buffer (Qiagen) was used to bind cDNA to the columns, and steps 9–11 from the Zymo plasmid miniprep kit were performed according to the manufacturer’s instructions. qRT-PCR was performed to test the expression of the 17 fimbriae in duplicate 25 µl reactions using 30 ng cDNA template, 150 nM each primer (Table S1) and 12.5 µl Maxima SYBR Green/fluorescein qPCR master mix (Thermo Scientific). .. Amplification of specific genes was performed over 40 cycles with a CFX Connect thermal cycler (Bio-Rad).

    Concentration Assay:

    Article Title: The pneumococcal σX activator, ComW, is a DNA-binding protein critical for natural transformation
    Article Snippet: .. Plasmids were isolated using the ZymoPURE Plasmid Miniprep Kit (Zymo Research) and concentration was measured using a ND-1000 Spectrophotometer (Nanodrop). .. Mutations were confirmed by Sanger DNA sequencing (University of Illinois at Chicago Sequencing Core (UICSQC)).

    Spectrophotometry:

    Article Title: The pneumococcal σX activator, ComW, is a DNA-binding protein critical for natural transformation
    Article Snippet: .. Plasmids were isolated using the ZymoPURE Plasmid Miniprep Kit (Zymo Research) and concentration was measured using a ND-1000 Spectrophotometer (Nanodrop). .. Mutations were confirmed by Sanger DNA sequencing (University of Illinois at Chicago Sequencing Core (UICSQC)).

    Expressing:

    Article Title: Characterization of 17 chaperone-usher fimbriae encoded by Proteus mirabilis reveals strong conservation
    Article Snippet: .. PCR using primers targeting rpoA (RNA polymerase A) with cDNA templates with or without reverse transcriptase treatment was used to confirm lack of genomic contamination. cDNA was then purified and concentrated using a plasmid miniprep kit (Zymo) with the following modifications: PB buffer (Qiagen) was used to bind cDNA to the columns, and steps 9–11 from the Zymo plasmid miniprep kit were performed according to the manufacturer’s instructions. qRT-PCR was performed to test the expression of the 17 fimbriae in duplicate 25 µl reactions using 30 ng cDNA template, 150 nM each primer (Table S1) and 12.5 µl Maxima SYBR Green/fluorescein qPCR master mix (Thermo Scientific). .. Amplification of specific genes was performed over 40 cycles with a CFX Connect thermal cycler (Bio-Rad).

    Polymerase Chain Reaction:

    Article Title: Characterization of 17 chaperone-usher fimbriae encoded by Proteus mirabilis reveals strong conservation
    Article Snippet: .. PCR using primers targeting rpoA (RNA polymerase A) with cDNA templates with or without reverse transcriptase treatment was used to confirm lack of genomic contamination. cDNA was then purified and concentrated using a plasmid miniprep kit (Zymo) with the following modifications: PB buffer (Qiagen) was used to bind cDNA to the columns, and steps 9–11 from the Zymo plasmid miniprep kit were performed according to the manufacturer’s instructions. qRT-PCR was performed to test the expression of the 17 fimbriae in duplicate 25 µl reactions using 30 ng cDNA template, 150 nM each primer (Table S1) and 12.5 µl Maxima SYBR Green/fluorescein qPCR master mix (Thermo Scientific). .. Amplification of specific genes was performed over 40 cycles with a CFX Connect thermal cycler (Bio-Rad).

    Plasmid Preparation:

    Article Title: Construction of a random circular permutation library using an engineered transposon.
    Article Snippet: .. Circular permutation is an important protein engineering tool used to create sequence diversity of a protein by changing its linear order of amino acid sequence. .. Circular permutation is an important protein engineering tool used to create sequence diversity of a protein by changing its linear order of amino acid sequence.

    Article Title: The cell non-autonomous function of ATG-18 is essential for neuroendocrine regulation of Caenorhabditis elegans lifespan
    Article Snippet: .. Plasmid DNA was prepared for microinjection by using Zymo Plasmid Miniprep Kit (Zymo Research, Irvine , CA ). ..

    Article Title: High-Throughput Screening of Coenzyme Preference Change of Thermophilic 6-Phosphogluconate Dehydrogenase from NADP+ to NAD+
    Article Snippet: .. The identified colonies embedded in the agarose gel were taken out by a sterile toothstick and then suspended in 200 μL of the P1 buffer of Zymo ZR Plasmid Miniprep™ kit followed by the Zymo ZR plasmid purification protocol. .. A tiny amount of plasmid extracted from the agarose gel was transformed into E. coli TOP10 for plasmid amplification, purification and DNA sequencing.

    Article Title: Characterization of 17 chaperone-usher fimbriae encoded by Proteus mirabilis reveals strong conservation
    Article Snippet: .. PCR using primers targeting rpoA (RNA polymerase A) with cDNA templates with or without reverse transcriptase treatment was used to confirm lack of genomic contamination. cDNA was then purified and concentrated using a plasmid miniprep kit (Zymo) with the following modifications: PB buffer (Qiagen) was used to bind cDNA to the columns, and steps 9–11 from the Zymo plasmid miniprep kit were performed according to the manufacturer’s instructions. qRT-PCR was performed to test the expression of the 17 fimbriae in duplicate 25 µl reactions using 30 ng cDNA template, 150 nM each primer (Table S1) and 12.5 µl Maxima SYBR Green/fluorescein qPCR master mix (Thermo Scientific). .. Amplification of specific genes was performed over 40 cycles with a CFX Connect thermal cycler (Bio-Rad).

    Article Title: Kinetics and Fidelity of Psychrophilic DNA Polymerases
    Article Snippet: .. All cloning products were first subcloned in NEB-5α strains and then purified using ZymoPURE plasmid miniprep kits (Zymo Research). .. The sequences of the expression constructs were all verified with Sanger sequencing by Sequetech.

    Article Title: Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping
    Article Snippet: .. Samples taken throughout the cultivation were immediately mixed with an excess of ice-cold, sterile PBS for rapid cooling and then centrifuged (10 min, 4’000 rcf, 4 °C), and cell pellets were frozen on dry ice until extraction of plasmid DNA was performed using a commercial kit (ZymoPURE Miniprep Kit, Zymo Research) and stored at -20 °C until further use. .. NGS Sample Preparation Plasmid DNA isolated from culture samples was digested with Nco I and Sac I (37 °C, 4 h).

    Article Title: The pneumococcal σX activator, ComW, is a DNA-binding protein critical for natural transformation
    Article Snippet: .. Plasmids were isolated using the ZymoPURE Plasmid Miniprep Kit (Zymo Research) and concentration was measured using a ND-1000 Spectrophotometer (Nanodrop). .. Mutations were confirmed by Sanger DNA sequencing (University of Illinois at Chicago Sequencing Core (UICSQC)).

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  • News
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  • Team
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  • Contact
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  • Bioz vStars
  • 99
    Zymo Research plasmid miniprep kit
    Plasmid Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid miniprep kit/product/Zymo Research
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    plasmid miniprep kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

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