zymopure plasmid miniprep kit  (Zymo Research)


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    Name:
    ZymoPURE Plasmid Miniprep Kit
    Description:
    The ZymoPURE Plasmid Miniprep Kit features a spin column based method for the purification of up to 100 µg of ultra pure endotoxin free plasmid DNA in less than 15 minutes The unique spin column design also provides zero buffer retention and a low elution volume ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA up to 3 µg µl In addition the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins salt protein and RNA The result is plasmid DNA suitable for transfection restriction endonuclease digestion bacterial transformation PCR amplification DNA sequencing and other sensitive downstream applications As an added convenience the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error free visualization and identification of complete bacterial cell lysis and neutralization
    Catalog Number:
    d4208t
    Product Aliases:
    ZymoPURE II Plasmid Miniprep Kit
    Price:
    None
    Applications:
    Plasmid DNA Purification
    Size:
    10 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research zymopure plasmid miniprep kit
    ZymoPURE Plasmid Miniprep Kit
    The ZymoPURE Plasmid Miniprep Kit features a spin column based method for the purification of up to 100 µg of ultra pure endotoxin free plasmid DNA in less than 15 minutes The unique spin column design also provides zero buffer retention and a low elution volume ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA up to 3 µg µl In addition the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins salt protein and RNA The result is plasmid DNA suitable for transfection restriction endonuclease digestion bacterial transformation PCR amplification DNA sequencing and other sensitive downstream applications As an added convenience the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error free visualization and identification of complete bacterial cell lysis and neutralization
    https://www.bioz.com/result/zymopure plasmid miniprep kit/product/Zymo Research
    Average 95 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    zymopure plasmid miniprep kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Transduction:

    Article Title: Identification of the P3 Promoter and Distinct Roles of the Two Promoters of the SaeRS Two-Component System in Staphylococcus aureus ▿
    Article Snippet: Plasmids and genomic DNA were extracted with a Zippy plasmid miniprep kit (Zymo Research) and a GenElute bacterial genomic DNA kit (Sigma-Aldrich), respectively, according to the manufacturers' instructions. .. Transduction into target strains of S. aureus was carried out with φ85 ( ).

    Clone Assay:

    Article Title: Contribution of non-coding mutations to RPGRIP1-mediated inherited retinal degeneration
    Article Snippet: Paragraph title: Polymerase chain reactions (PCR), cloning and site-directed mutagenesis ... Plasmid DNA from single colonies was extracted with miniprep kits (ZymoPURE, Zymo Research) and analyzed by restriction enzyme digestion with BsrGI (NE Biolabs, Ipswich, MA) and Sanger sequencing.

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ). .. The xylose repressor and promoter of pRBBm34 were replaced with a hyperspank promoter and lacI using circular polymerase extension cloning (CPEC).

    Article Title: Isolation and Characterization of the Etheostoma tallapoosae (Teleostei: Percidae) CENP-A Gene
    Article Snippet: .. Recombinant plasmids of cloned amplimers were isolated utilizing the Zippy Plasmid Miniprep Kit (Zymo Research). .. The fragments cloned in the pSMART plasmids were sequenced from both ends utilizing SL1 and SL2 primers (Lucigen).

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: Clones were transformed to TOP10 E. coli cells and plated on LB+Ampicillin+X-gal plates. .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Article Title: Deletion of a specific exon in the voltage-gated calcium channel gene cacophony disrupts locomotion in Drosophila larvae
    Article Snippet: The synthesized sequence was cut out of its cloning vector using Age I and Kpn I and shuttled into cac 1 . .. The transformation constructs were prepared for injection using ZymoPURE Plasmid Mini Prep Kit (Zymo Research, Irvine, CA, USA) and injected at a concentration of 500 ng µl−1 .

    Article Title: Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies
    Article Snippet: PCR fragments were cloned into pCR-Blunt II-TOPO vector (Thermo Fisher). .. Plasmid DNA was isolated using a ZymoPure Plasmid Miniprep Kit (Zymo Research) and the plasmid inserts were sequenced with the M13 forward and reverse primers and the RV primers [ ].

    Centrifugation:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: Protoplasts were collected by centrifugation at 1300 r.p.m. for 10 min, followed by a wash with 5 ml 1x SMMP. .. Each sample was transformed with 3 μg freshly prepared plasmid DNA (Zippy plasmid miniprep kit, Zymo research) and 1.5 ml of a 20% PEG 8000 (Promega) solution in 1x SMM (2.32 g l-1 L -malic acid (Alfa Aesar), 1.6 g l-1 NaOH, 4.06 g l-1 MgCl2 •6H2 0, 171.16 g l-1 sucrose).

    Amplification:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: The gene encoding SuperFolder GFP was PCR amplified from the pTRC99a gfp SF (lab stock) template using the oligonucleotides Spe I gfp for and gfp Sph I rev (Supplementary Table ). .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ).

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: The randomly amplified xylose isomerase genes were digested with EcoRI and XhoI (New England BioLabs, Ipswich, MA) and ligated into the EcoRI and XhoI sites of p416-GPD. .. The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1.

    Article Title: Identification of the P3 Promoter and Distinct Roles of the Two Promoters of the SaeRS Two-Component System in Staphylococcus aureus ▿
    Article Snippet: Paragraph title: DNA manipulation and amplification. ... Plasmids and genomic DNA were extracted with a Zippy plasmid miniprep kit (Zymo Research) and a GenElute bacterial genomic DNA kit (Sigma-Aldrich), respectively, according to the manufacturers' instructions.

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: After amplification and purification with PCR purification kit, second set of PCR was performed in order to increase the length of the overlap regions to increase efficiency of the ligation. .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Article Title: Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies
    Article Snippet: Molecular coning and Sanger sequencing for quasispecies analysis An 904-nt fragment of the E1 gene (nt 8672–9577 in the RV genome) was amplified from RNA isolated from a clinical sample or viral stock using the RV genotyping primers [ ] and Platinum SuperFi DNA Polymerase ( > 100x Taq fidelity, Thermo Fisher). .. Plasmid DNA was isolated using a ZymoPure Plasmid Miniprep Kit (Zymo Research) and the plasmid inserts were sequenced with the M13 forward and reverse primers and the RV primers [ ].

    Synthesized:

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: A yeast codon-optimized version of the xylose isomerase gene ( xylA ) from Piromyces sp. was synthesized by Blue Heron (Bothell, WA) and cloned into the Mumberg plasmid p416-GPD ( ) to form p416-GPD-XI. .. The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1.

    Article Title: Deletion of a specific exon in the voltage-gated calcium channel gene cacophony disrupts locomotion in Drosophila larvae
    Article Snippet: The synthesized sequence was cut out of its cloning vector using Age I and Kpn I and shuttled into cac 1 . .. The transformation constructs were prepared for injection using ZymoPURE Plasmid Mini Prep Kit (Zymo Research, Irvine, CA, USA) and injected at a concentration of 500 ng µl−1 .

    TA Cloning:

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: The final piece was cloned into PCR2.1 vector using TOPO TA cloning kit for subcloning (Thermo Fisher). .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Construct:

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: Paragraph title: Splicing constructs ... Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Article Title: Deletion of a specific exon in the voltage-gated calcium channel gene cacophony disrupts locomotion in Drosophila larvae
    Article Snippet: .. The transformation constructs were prepared for injection using ZymoPURE Plasmid Mini Prep Kit (Zymo Research, Irvine, CA, USA) and injected at a concentration of 500 ng µl−1 . .. Two sets of primers were designed to span a region that included the DsRed sequence at the 5′ end of cacophony and the 5′ homology arm (primers 5′ GT S and 5′ GT R; ) and the region spanning DsRed at the 3′ end of cacophony and 3′ homology arm (primers 3′ GT S and 3′ GT R; ).

    Incubation:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: Each sample was transformed with 3 μg freshly prepared plasmid DNA (Zippy plasmid miniprep kit, Zymo research) and 1.5 ml of a 20% PEG 8000 (Promega) solution in 1x SMM (2.32 g l-1 L -malic acid (Alfa Aesar), 1.6 g l-1 NaOH, 4.06 g l-1 MgCl2 •6H2 0, 171.16 g l-1 sucrose). .. Samples were incubated with gentle mixing for 2 min, followed by addition of 5 ml 1x SMMP and centrifugation to collect the transformed protoplasts.

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis. .. Standard Sanger sequencing of isolated plasmids was used to verify identity (Genewiz).

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: Transformation, DNA isolation, and analysis Modified plasmid DNA recovered from in vitro reactions were transformed in 50 µl of DH5α competent E. coli (Thermo Fisher Scientific Wilmington, DE) via heat shock methodology, after which 150 µl of undiluted cells were plated on media containing X-gal and kanamycin and incubated overnight at 37 °C. .. Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA).

    Modification:

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: Transformation, DNA isolation, and analysis Modified plasmid DNA recovered from in vitro reactions were transformed in 50 µl of DH5α competent E. coli (Thermo Fisher Scientific Wilmington, DE) via heat shock methodology, after which 150 µl of undiluted cells were plated on media containing X-gal and kanamycin and incubated overnight at 37 °C. .. Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA).

    Article Title: Identification of the P3 Promoter and Distinct Roles of the Two Promoters of the SaeRS Two-Component System in Staphylococcus aureus ▿
    Article Snippet: Unless stated otherwise, all restriction enzymes and DNA modification enzymes were purchased from New England BioLabs. .. Plasmids and genomic DNA were extracted with a Zippy plasmid miniprep kit (Zymo Research) and a GenElute bacterial genomic DNA kit (Sigma-Aldrich), respectively, according to the manufacturers' instructions.

    Transformation Assay:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Each sample was transformed with 3 μg freshly prepared plasmid DNA (Zippy plasmid miniprep kit, Zymo research) and 1.5 ml of a 20% PEG 8000 (Promega) solution in 1x SMM (2.32 g l-1 L -malic acid (Alfa Aesar), 1.6 g l-1 NaOH, 4.06 g l-1 MgCl2 •6H2 0, 171.16 g l-1 sucrose). .. Samples were incubated with gentle mixing for 2 min, followed by addition of 5 ml 1x SMMP and centrifugation to collect the transformed protoplasts.

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: Paragraph title: Transformation of SR7 and verification ... Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis.

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: Paragraph title: Transformation, DNA isolation, and analysis ... Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA).

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: The mutant plasmid library was transformed into E. coli DH10β using standard electroporation protocols ( ). .. The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1.

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: Clones were transformed to TOP10 E. coli cells and plated on LB+Ampicillin+X-gal plates. .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Article Title: Deletion of a specific exon in the voltage-gated calcium channel gene cacophony disrupts locomotion in Drosophila larvae
    Article Snippet: .. The transformation constructs were prepared for injection using ZymoPURE Plasmid Mini Prep Kit (Zymo Research, Irvine, CA, USA) and injected at a concentration of 500 ng µl−1 . .. Two sets of primers were designed to span a region that included the DsRed sequence at the 5′ end of cacophony and the 5′ homology arm (primers 5′ GT S and 5′ GT R; ) and the region spanning DsRed at the 3′ end of cacophony and 3′ homology arm (primers 3′ GT S and 3′ GT R; ).

    Gel Purification:

    Article Title: Solving a Hamiltonian Path Problem with a bacterial computer
    Article Snippet: Plasmid preparations were conducted using either the Zippy Plasmid Miniprep Kit from Zymo Research or the QIAprep Spin Miniprep Kit from Qiagen. .. Gel fragment purifications were performed with either the Zymo Research Zymoclean DNA Recovery Kit or the Qiagen QiaExII polyacrylamide gel purification kit.

    Electroporation:

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: The mutant plasmid library was transformed into E. coli DH10β using standard electroporation protocols ( ). .. The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1.

    Article Title: Identification of the P3 Promoter and Distinct Roles of the Two Promoters of the SaeRS Two-Component System in Staphylococcus aureus ▿
    Article Snippet: Plasmids and genomic DNA were extracted with a Zippy plasmid miniprep kit (Zymo Research) and a GenElute bacterial genomic DNA kit (Sigma-Aldrich), respectively, according to the manufacturers' instructions. .. Plasmid DNA was introduced into E. coli by the method of Hanahan and Meselson ( ) and into S. aureus RN4220 by electroporation ( ) with a gene pulser (Bio-Rad).

    Transfection:

    Article Title: Contribution of non-coding mutations to RPGRIP1-mediated inherited retinal degeneration
    Article Snippet: Plasmid DNA from single colonies was extracted with miniprep kits (ZymoPURE, Zymo Research) and analyzed by restriction enzyme digestion with BsrGI (NE Biolabs, Ipswich, MA) and Sanger sequencing. .. Colonies with the correct sequence and restriction enzyme pattern were then sub-cloned into the pCS2+GW vector (kind gift from Dr Erica Davis) via Gateway LR clonase II (Thermo Fisher) and similar analyses as before was done to isolate vectors with the appropriate inserts for transfection experiments.

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research). .. A total of 400 ng TOPO vector was transfected into HEK293 cells and harvested after 48 h.

    Ligation:

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: After amplification and purification with PCR purification kit, second set of PCR was performed in order to increase the length of the overlap regions to increase efficiency of the ligation. .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Generated:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: The pXyl empty plasmid was generated by adding the DNA sequence 5ʹ-atttaatgttgatgaaagctggtctggtgtcaaaaataatga-3ʹ between the Spe I and Sph I restriction enzyme sites. .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ).

    DNA Sequencing:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ). .. The xylose repressor and promoter of pRBBm34 were replaced with a hyperspank promoter and lacI using circular polymerase extension cloning (CPEC).

    Article Title: Isolation and Characterization of the Etheostoma tallapoosae (Teleostei: Percidae) CENP-A Gene
    Article Snippet: Paragraph title: DNA Sequencing and Sequence Analysis ... Recombinant plasmids of cloned amplimers were isolated utilizing the Zippy Plasmid Miniprep Kit (Zymo Research).

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA). .. Modifications made to plasmid DNA were evaluated after DNA sequencing (GeneWiz, South Plainfield, NJ) and disruption patterns were assessed using SnapGene software.

    Polymerase Chain Reaction:

    Article Title: Contribution of non-coding mutations to RPGRIP1-mediated inherited retinal degeneration
    Article Snippet: Paragraph title: Polymerase chain reactions (PCR), cloning and site-directed mutagenesis ... Plasmid DNA from single colonies was extracted with miniprep kits (ZymoPURE, Zymo Research) and analyzed by restriction enzyme digestion with BsrGI (NE Biolabs, Ipswich, MA) and Sanger sequencing.

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: The resulting PCR product and pRBBm34 plasmid were digested with Spe I and Sph I, and resulting fragments ligated to create pXyl gfp . .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ).

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: For colony PCR, colonies were picked and boiled for 10 min to serve as template for the PCR reaction using OneTaq Quick-Load Master Mix (NEB) and appropriate sequencing primers (Supplementary Table ). .. Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis.

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: Error-prone PCR of the xylose isomerase gene was conducted with the primer set S004 xylA-f (5′-CCGGGCTGCAGGAATTCATG-3′) and S004 xylA-r (5′-GCGTGACATAACTAATTACATGACTCGAGTTA-3′) using a GeneMorph II Random Mutagenesis Kit (Stratagene, La Jolla, CA). .. The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1.

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: CMV promoter, Ad81 exon, ACTN exon and polyA signal were added using overlap extension PCR. .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Article Title: Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies
    Article Snippet: PCR fragments were cloned into pCR-Blunt II-TOPO vector (Thermo Fisher). .. Plasmid DNA was isolated using a ZymoPure Plasmid Miniprep Kit (Zymo Research) and the plasmid inserts were sequenced with the M13 forward and reverse primers and the RV primers [ ].

    Injection:

    Article Title: Deletion of a specific exon in the voltage-gated calcium channel gene cacophony disrupts locomotion in Drosophila larvae
    Article Snippet: .. The transformation constructs were prepared for injection using ZymoPURE Plasmid Mini Prep Kit (Zymo Research, Irvine, CA, USA) and injected at a concentration of 500 ng µl−1 . .. Two sets of primers were designed to span a region that included the DsRed sequence at the 5′ end of cacophony and the 5′ homology arm (primers 5′ GT S and 5′ GT R; ) and the region spanning DsRed at the 3′ end of cacophony and 3′ homology arm (primers 3′ GT S and 3′ GT R; ).

    Recombinant:

    Article Title: Isolation and Characterization of the Etheostoma tallapoosae (Teleostei: Percidae) CENP-A Gene
    Article Snippet: .. Recombinant plasmids of cloned amplimers were isolated utilizing the Zippy Plasmid Miniprep Kit (Zymo Research). .. The fragments cloned in the pSMART plasmids were sequenced from both ends utilizing SL1 and SL2 primers (Lucigen).

    DNA Extraction:

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: Paragraph title: Transformation, DNA isolation, and analysis ... Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA).

    Molecular Cloning:

    Article Title: Solving a Hamiltonian Path Problem with a bacterial computer
    Article Snippet: Construction of HPP parts and devices Materials used in molecular cloning procedures were as follows. .. Plasmid preparations were conducted using either the Zippy Plasmid Miniprep Kit from Zymo Research or the QIAprep Spin Miniprep Kit from Qiagen.

    Mutagenesis:

    Article Title: Contribution of non-coding mutations to RPGRIP1-mediated inherited retinal degeneration
    Article Snippet: Paragraph title: Polymerase chain reactions (PCR), cloning and site-directed mutagenesis ... Plasmid DNA from single colonies was extracted with miniprep kits (ZymoPURE, Zymo Research) and analyzed by restriction enzyme digestion with BsrGI (NE Biolabs, Ipswich, MA) and Sanger sequencing.

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: Paragraph title: Construction of a xylA mutant library. ... The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1.

    Isolation:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis. .. Standard Sanger sequencing of isolated plasmids was used to verify identity (Genewiz).

    Article Title: Isolation and Characterization of the Etheostoma tallapoosae (Teleostei: Percidae) CENP-A Gene
    Article Snippet: .. Recombinant plasmids of cloned amplimers were isolated utilizing the Zippy Plasmid Miniprep Kit (Zymo Research). .. The fragments cloned in the pSMART plasmids were sequenced from both ends utilizing SL1 and SL2 primers (Lucigen).

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: .. Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA). .. Modifications made to plasmid DNA were evaluated after DNA sequencing (GeneWiz, South Plainfield, NJ) and disruption patterns were assessed using SnapGene software.

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: .. The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1. .. Yeast transformation was conducted using a Frozen EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA) according to the manufacturer's instructions to achieve a one to three times coverage of the original mutagenesis library.

    Article Title: Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies
    Article Snippet: .. Plasmid DNA was isolated using a ZymoPure Plasmid Miniprep Kit (Zymo Research) and the plasmid inserts were sequenced with the M13 forward and reverse primers and the RV primers [ ]. ..

    Subcloning:

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: The final piece was cloned into PCR2.1 vector using TOPO TA cloning kit for subcloning (Thermo Fisher). .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Microscopy:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: Protoplast formation was performed at 37 °C for 10 min at 70 r.p.m. and was visualized by microscopy. .. Each sample was transformed with 3 μg freshly prepared plasmid DNA (Zippy plasmid miniprep kit, Zymo research) and 1.5 ml of a 20% PEG 8000 (Promega) solution in 1x SMM (2.32 g l-1 L -malic acid (Alfa Aesar), 1.6 g l-1 NaOH, 4.06 g l-1 MgCl2 •6H2 0, 171.16 g l-1 sucrose).

    Purification:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ). .. The xylose repressor and promoter of pRBBm34 were replaced with a hyperspank promoter and lacI using circular polymerase extension cloning (CPEC).

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis. .. Purified plasmids were also backtransformed into E. coli DH5α, which were subsequently plated on LB agar containing 100 μg ml–1 carbenicillin.

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: After amplification and purification with PCR purification kit, second set of PCR was performed in order to increase the length of the overlap regions to increase efficiency of the ligation. .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research).

    Sequencing:

    Article Title: Contribution of non-coding mutations to RPGRIP1-mediated inherited retinal degeneration
    Article Snippet: .. Plasmid DNA from single colonies was extracted with miniprep kits (ZymoPURE, Zymo Research) and analyzed by restriction enzyme digestion with BsrGI (NE Biolabs, Ipswich, MA) and Sanger sequencing. .. Essential splice-site mutations were introduced by site-directed mutagenesis (QuickChange II Site Directed mutagenesis kit, Agilent Technologies) and verified by Sanger sequencing.

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ). .. The xylose repressor and promoter of pRBBm34 were replaced with a hyperspank promoter and lacI using circular polymerase extension cloning (CPEC).

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: For colony PCR, colonies were picked and boiled for 10 min to serve as template for the PCR reaction using OneTaq Quick-Load Master Mix (NEB) and appropriate sequencing primers (Supplementary Table ). .. Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis.

    Article Title: Isolation and Characterization of the Etheostoma tallapoosae (Teleostei: Percidae) CENP-A Gene
    Article Snippet: Paragraph title: DNA Sequencing and Sequence Analysis ... Recombinant plasmids of cloned amplimers were isolated utilizing the Zippy Plasmid Miniprep Kit (Zymo Research).

    Article Title: Deletion of a specific exon in the voltage-gated calcium channel gene cacophony disrupts locomotion in Drosophila larvae
    Article Snippet: The synthesized sequence was cut out of its cloning vector using Age I and Kpn I and shuttled into cac 1 . .. The transformation constructs were prepared for injection using ZymoPURE Plasmid Mini Prep Kit (Zymo Research, Irvine, CA, USA) and injected at a concentration of 500 ng µl−1 .

    Article Title: Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies
    Article Snippet: Paragraph title: Molecular coning and Sanger sequencing for quasispecies analysis ... Plasmid DNA was isolated using a ZymoPure Plasmid Miniprep Kit (Zymo Research) and the plasmid inserts were sequenced with the M13 forward and reverse primers and the RV primers [ ].

    Plasmid Preparation:

    Article Title: Solving a Hamiltonian Path Problem with a bacterial computer
    Article Snippet: .. Plasmid preparations were conducted using either the Zippy Plasmid Miniprep Kit from Zymo Research or the QIAprep Spin Miniprep Kit from Qiagen. .. Gel fragment purifications were performed with either the Zymo Research Zymoclean DNA Recovery Kit or the Qiagen QiaExII polyacrylamide gel purification kit.

    Article Title: Contribution of non-coding mutations to RPGRIP1-mediated inherited retinal degeneration
    Article Snippet: .. Plasmid DNA from single colonies was extracted with miniprep kits (ZymoPURE, Zymo Research) and analyzed by restriction enzyme digestion with BsrGI (NE Biolabs, Ipswich, MA) and Sanger sequencing. .. Essential splice-site mutations were introduced by site-directed mutagenesis (QuickChange II Site Directed mutagenesis kit, Agilent Technologies) and verified by Sanger sequencing.

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Each sample was transformed with 3 μg freshly prepared plasmid DNA (Zippy plasmid miniprep kit, Zymo research) and 1.5 ml of a 20% PEG 8000 (Promega) solution in 1x SMM (2.32 g l-1 L -malic acid (Alfa Aesar), 1.6 g l-1 NaOH, 4.06 g l-1 MgCl2 •6H2 0, 171.16 g l-1 sucrose). .. Samples were incubated with gentle mixing for 2 min, followed by addition of 5 ml 1x SMMP and centrifugation to collect the transformed protoplasts.

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ). .. The xylose repressor and promoter of pRBBm34 were replaced with a hyperspank promoter and lacI using circular polymerase extension cloning (CPEC).

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis. .. Standard Sanger sequencing of isolated plasmids was used to verify identity (Genewiz).

    Article Title: Isolation and Characterization of the Etheostoma tallapoosae (Teleostei: Percidae) CENP-A Gene
    Article Snippet: .. Recombinant plasmids of cloned amplimers were isolated utilizing the Zippy Plasmid Miniprep Kit (Zymo Research). .. The fragments cloned in the pSMART plasmids were sequenced from both ends utilizing SL1 and SL2 primers (Lucigen).

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: .. Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA). .. Modifications made to plasmid DNA were evaluated after DNA sequencing (GeneWiz, South Plainfield, NJ) and disruption patterns were assessed using SnapGene software.

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: .. The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1. .. Yeast transformation was conducted using a Frozen EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA) according to the manufacturer's instructions to achieve a one to three times coverage of the original mutagenesis library.

    Article Title: Identification of the P3 Promoter and Distinct Roles of the Two Promoters of the SaeRS Two-Component System in Staphylococcus aureus ▿
    Article Snippet: .. Plasmids and genomic DNA were extracted with a Zippy plasmid miniprep kit (Zymo Research) and a GenElute bacterial genomic DNA kit (Sigma-Aldrich), respectively, according to the manufacturers' instructions. .. Plasmid DNA was introduced into E. coli by the method of Hanahan and Meselson ( ) and into S. aureus RN4220 by electroporation ( ) with a gene pulser (Bio-Rad).

    Article Title: Widespread intra-dependencies in the removal of introns from human transcripts
    Article Snippet: .. Plasmids were extracted using Zippy Plasmid Miniprep Kit (Zymo Research). .. A total of 400 ng TOPO vector was transfected into HEK293 cells and harvested after 48 h.

    Article Title: Deletion of a specific exon in the voltage-gated calcium channel gene cacophony disrupts locomotion in Drosophila larvae
    Article Snippet: .. The transformation constructs were prepared for injection using ZymoPURE Plasmid Mini Prep Kit (Zymo Research, Irvine, CA, USA) and injected at a concentration of 500 ng µl−1 . .. Two sets of primers were designed to span a region that included the DsRed sequence at the 5′ end of cacophony and the 5′ homology arm (primers 5′ GT S and 5′ GT R; ) and the region spanning DsRed at the 3′ end of cacophony and 3′ homology arm (primers 3′ GT S and 3′ GT R; ).

    Article Title: Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies
    Article Snippet: .. Plasmid DNA was isolated using a ZymoPure Plasmid Miniprep Kit (Zymo Research) and the plasmid inserts were sequenced with the M13 forward and reverse primers and the RV primers [ ]. ..

    Software:

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA). .. Modifications made to plasmid DNA were evaluated after DNA sequencing (GeneWiz, South Plainfield, NJ) and disruption patterns were assessed using SnapGene software.

    Functional Assay:

    Article Title: Isolation and Characterization of the Etheostoma tallapoosae (Teleostei: Percidae) CENP-A Gene
    Article Snippet: Recombinant plasmids of cloned amplimers were isolated utilizing the Zippy Plasmid Miniprep Kit (Zymo Research). .. Recombinant plasmids of cloned amplimers were isolated utilizing the Zippy Plasmid Miniprep Kit (Zymo Research).

    Selection:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: The pXyl gfp plasmid is based on the pRBBm34 shuttle vector (purchased from Addgene), which contains a xylose-inducible promoter, the xylose repressor ( xylR ) under control of its native B. megaterium promoter, ampicillin resistance marker (Ampr ) for selection in E. coli , tetracycline resistance marker (Tetr ) for selection in B. megaterium , and two origins of replication. .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ).

    Article Title: Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae
    Article Snippet: The resulting E. coli library was harvested from the petri dishes, isolated using the Zippy Plasmid Miniprep Kit (Zymo Research, Irvine, CA), and retransformed into S. cerevisiae BY4741-S1. .. The yeast libraries were allowed to grow for 3 days on plates before being scraped off and harvested for selection.

    In Vitro:

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: Transformation, DNA isolation, and analysis Modified plasmid DNA recovered from in vitro reactions were transformed in 50 µl of DH5α competent E. coli (Thermo Fisher Scientific Wilmington, DE) via heat shock methodology, after which 150 µl of undiluted cells were plated on media containing X-gal and kanamycin and incubated overnight at 37 °C. .. Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA).

    Concentration Assay:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: Lysozyme (Sigma) was added to a concentration of 30 μg ml–1 , which was tenfold lower than used in previous methods . .. Each sample was transformed with 3 μg freshly prepared plasmid DNA (Zippy plasmid miniprep kit, Zymo research) and 1.5 ml of a 20% PEG 8000 (Promega) solution in 1x SMM (2.32 g l-1 L -malic acid (Alfa Aesar), 1.6 g l-1 NaOH, 4.06 g l-1 MgCl2 •6H2 0, 171.16 g l-1 sucrose).

    Article Title: Deletion of a specific exon in the voltage-gated calcium channel gene cacophony disrupts locomotion in Drosophila larvae
    Article Snippet: .. The transformation constructs were prepared for injection using ZymoPURE Plasmid Mini Prep Kit (Zymo Research, Irvine, CA, USA) and injected at a concentration of 500 ng µl−1 . .. Two sets of primers were designed to span a region that included the DsRed sequence at the 5′ end of cacophony and the 5′ homology arm (primers 5′ GT S and 5′ GT R; ) and the region spanning DsRed at the 3′ end of cacophony and 3′ homology arm (primers 3′ GT S and 3′ GT R; ).

    Marker:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: The pXyl gfp plasmid is based on the pRBBm34 shuttle vector (purchased from Addgene), which contains a xylose-inducible promoter, the xylose repressor ( xylR ) under control of its native B. megaterium promoter, ampicillin resistance marker (Ampr ) for selection in E. coli , tetracycline resistance marker (Tetr ) for selection in B. megaterium , and two origins of replication. .. Plasmids were purified (Zippy plasmid miniprep kit, Zymo research) prior to verification by DNA sequencing (Genewiz) using appropriate sequencing oligonucleotide primers (Supplementary Table ).

    Lysis:

    Article Title: Engineered microbial biofuel production and recovery under supercritical carbon dioxide
    Article Snippet: .. Plasmid DNA was isolated from SR7 using a standard plasmid isolation protocol and materials (Zippy plasmid miniprep kit, Zymo research), except 10 μg ml–1 lysozyme was added for the initial resuspension step with a 1 h incubation at 37 °C to promote cell lysis. .. Standard Sanger sequencing of isolated plasmids was used to verify identity (Genewiz).

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    Zymo Research zymopure plasmid miniprep kit
    Zymopure Plasmid Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zymopure plasmid miniprep kit/product/Zymo Research
    Average 95 stars, based on 3 article reviews
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    zymopure plasmid miniprep kit - by Bioz Stars, 2020-02
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