zymospin chip kit  (Zymo Research)


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    Name:
    Zymo Spin ChIP Kit
    Description:
    The Zymo Spin ChIP Kit from Zymo Research provides a streamlined ChIP procedure for investigating protein DNA interactions that have been fixed in their natural state and can be used to effectively identify binding sites for transcription factors co factors and other DNA regulatory proteins
    Catalog Number:
    d5209
    Price:
    None
    Applications:
    Chromatin Immunoprecipitation
    Size:
    10 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research zymospin chip kit
    Zymo Spin ChIP Kit
    The Zymo Spin ChIP Kit from Zymo Research provides a streamlined ChIP procedure for investigating protein DNA interactions that have been fixed in their natural state and can be used to effectively identify binding sites for transcription factors co factors and other DNA regulatory proteins
    https://www.bioz.com/result/zymospin chip kit/product/Zymo Research
    Average 98 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    zymospin chip kit - by Bioz Stars, 2020-10
    98/100 stars

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    Related Articles

    Sample Prep:

    Article Title: Comprehensive epigenetic landscape of rheumatoid arthritis fibroblast-like synoviocytes
    Article Snippet: .. The sample preparation for ChIP-seq was performed using the Zymo-spin ChIP kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions. .. Raw ChIP-seq reads of 11 RA and 11 OA samples, each with six histone modifications (H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3, and H3K36me3) and input data, were mapped to hg19 genome using bwa (0.7.7-r441).

    Isolation:

    Article Title: Priming of transcriptional memory responses via the chromatin accessibility landscape in T cells
    Article Snippet: .. The remaining sonicated DNA (FAIRE DNA) was directly isolated by phenol-chloroform extraction and purified using the Zymo-SpinTM I kit (Zymo Research). .. 50 ng of FAIRE DNA and the corresponding total input DNA were used to prepare the FAIRE-seq DNA library using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (New England BioLabs Inc., Ipswich, MA) according to the manufacturer’s instructions.

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: .. Cells isolated from NT and TT through ED were subjected to ChIP analysis using Zymo-Spin ChIP kit (Zymo Research) as per manufacturer’s instructions. .. Briefly, after cell isolation by ED, nuclear lysate was prepared as per the protocol and sonicated using Omni Sonic Ruptor 400 Ultrasonic Homogenizer (OMNI International, GA, USA) to make small DNA fragments ranging from 100 to 1000 base pairs and then incubated with ChIP grade anti-Histone H3 (tri methyl K9) rabbit mAb (Abcam, Cambridge, United Kingdom) and anti-Histone H3 (tri methyl K27) rabbit mAb (Abcam).

    Article Title: Lysine-Specific Histone Demethylase 1A Regulates Macrophage Polarization and Checkpoint Molecules in the Tumor Microenvironment of Triple-Negative Breast Cancer
    Article Snippet: .. The remaining sonicated DNA (FAIRE DNA) was directly isolated by phenol-chloroform extraction and purified using the Zymo-SpinTM I kit (Zymo Research, Irvine, CA). .. Animal Studies Five-week-old female BALB/c mice were obtained from the Animal Resources Center (ARC), Perth, and allowed to acclimatize for 1 week in the containment suites at The John Curtin School of Medical Research (JCSMR).

    Purification:

    Article Title: Priming of transcriptional memory responses via the chromatin accessibility landscape in T cells
    Article Snippet: .. The remaining sonicated DNA (FAIRE DNA) was directly isolated by phenol-chloroform extraction and purified using the Zymo-SpinTM I kit (Zymo Research). .. 50 ng of FAIRE DNA and the corresponding total input DNA were used to prepare the FAIRE-seq DNA library using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (New England BioLabs Inc., Ipswich, MA) according to the manufacturer’s instructions.

    Article Title: Lysine-Specific Histone Demethylase 1A Regulates Macrophage Polarization and Checkpoint Molecules in the Tumor Microenvironment of Triple-Negative Breast Cancer
    Article Snippet: .. The remaining sonicated DNA (FAIRE DNA) was directly isolated by phenol-chloroform extraction and purified using the Zymo-SpinTM I kit (Zymo Research, Irvine, CA). .. Animal Studies Five-week-old female BALB/c mice were obtained from the Animal Resources Center (ARC), Perth, and allowed to acclimatize for 1 week in the containment suites at The John Curtin School of Medical Research (JCSMR).

    Sonication:

    Article Title: Priming of transcriptional memory responses via the chromatin accessibility landscape in T cells
    Article Snippet: .. The remaining sonicated DNA (FAIRE DNA) was directly isolated by phenol-chloroform extraction and purified using the Zymo-SpinTM I kit (Zymo Research). .. 50 ng of FAIRE DNA and the corresponding total input DNA were used to prepare the FAIRE-seq DNA library using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (New England BioLabs Inc., Ipswich, MA) according to the manufacturer’s instructions.

    Article Title: Lysine-Specific Histone Demethylase 1A Regulates Macrophage Polarization and Checkpoint Molecules in the Tumor Microenvironment of Triple-Negative Breast Cancer
    Article Snippet: .. The remaining sonicated DNA (FAIRE DNA) was directly isolated by phenol-chloroform extraction and purified using the Zymo-SpinTM I kit (Zymo Research, Irvine, CA). .. Animal Studies Five-week-old female BALB/c mice were obtained from the Animal Resources Center (ARC), Perth, and allowed to acclimatize for 1 week in the containment suites at The John Curtin School of Medical Research (JCSMR).

    Chromatin Immunoprecipitation:

    Article Title: H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes
    Article Snippet: .. The cross-linked cells were prepared for ChIP assay using Zymo-Spin ChIP kit (cat # D5209, Zymo Research, USA) following the manufacturer’s instructions. ..

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: .. Cells isolated from NT and TT through ED were subjected to ChIP analysis using Zymo-Spin ChIP kit (Zymo Research) as per manufacturer’s instructions. .. Briefly, after cell isolation by ED, nuclear lysate was prepared as per the protocol and sonicated using Omni Sonic Ruptor 400 Ultrasonic Homogenizer (OMNI International, GA, USA) to make small DNA fragments ranging from 100 to 1000 base pairs and then incubated with ChIP grade anti-Histone H3 (tri methyl K9) rabbit mAb (Abcam, Cambridge, United Kingdom) and anti-Histone H3 (tri methyl K27) rabbit mAb (Abcam).

    Article Title: Activation of STAT3 signaling is mediated by TFF1 silencing in gastric neoplasia
    Article Snippet: .. ChIP assay was performed using the Zymo-Spin ChIP Kit (Irvine, California, USA) following the manufacturer’s protocol. .. Chromatin solutions were immunoprecipitated with STAT3 antibody from cell signaling at 4 °C overnight.

    Article Title: The Translesion Polymerase Pol η Is Required for Efficient Epstein-Barr Virus Infectivity and Is Regulated by the Viral Deubiquitinating Enzyme BPLF1
    Article Snippet: .. Chromatin immunoprecipitations were performed using a Zymo-Spin ChIP kit (Zymo Research) essentially following the manufacturer's guidelines. .. 293EBV+ cells were transfected with BZLF1 and FLAG-tagged Pol η. Twenty-four hours after transfection, the cells were harvested, washed, and resuspended in 1 ml phosphate-buffered saline (PBS).

    Article Title: p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex
    Article Snippet: .. RNAPII ChIP experiments were performed using the ZymoSpin ChIP Kit (Zymo Research) according to instructions. .. A ChIP-grade anti-RNA-Polymerase II antibody (H-224, sc-9001, Santa Cruz Biotechnology) and as a control normal rabbit IgG (sc-2027, Santa Cruz Biotechnology) were used at concentrations of 2.5 µg/ChIP experiment.

    Article Title: Comprehensive epigenetic landscape of rheumatoid arthritis fibroblast-like synoviocytes
    Article Snippet: .. The sample preparation for ChIP-seq was performed using the Zymo-spin ChIP kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions. .. Raw ChIP-seq reads of 11 RA and 11 OA samples, each with six histone modifications (H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3, and H3K36me3) and input data, were mapped to hg19 genome using bwa (0.7.7-r441).

    ChIP-sequencing:

    Article Title: Comprehensive epigenetic landscape of rheumatoid arthritis fibroblast-like synoviocytes
    Article Snippet: .. The sample preparation for ChIP-seq was performed using the Zymo-spin ChIP kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions. .. Raw ChIP-seq reads of 11 RA and 11 OA samples, each with six histone modifications (H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3, and H3K36me3) and input data, were mapped to hg19 genome using bwa (0.7.7-r441).

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  • 98
    Zymo Research zymospin chip kit
    Zymospin Chip Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 98/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zymospin chip kit/product/Zymo Research
    Average 98 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    zymospin chip kit - by Bioz Stars, 2020-10
    98/100 stars
      Buy from Supplier

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