nucleocapsid  (Sino Biological)


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  • 94
    Name:
    Influenza B Nucleocapsid protein NP Gene ORF cDNA clone expression plasmid
    Description:
    Full length Clone DNA of Influenza B B Florida 4 2006 Nucleocapsid protein
    Catalog Number:
    vg40438-g
    Product Aliases:
    NP cDNA ORF Clone Influenza B, Nucleoprotein cDNA ORF Clone Influenza B
    Price:
    115.0
    Size:
    1Unit
    Category:
    cDNA Clone
    Molecule Name:
    NP,Nucleoprotein,Nucleocapsid
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    Structured Review

    Sino Biological nucleocapsid
    Full length Clone DNA of Influenza B B Florida 4 2006 Nucleocapsid protein
    https://www.bioz.com/result/nucleocapsid/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nucleocapsid - by Bioz Stars, 2021-02
    94/100 stars

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    Related Articles

    other:

    Article Title: T-cell responses to MERS coronavirus infection in people with occupational exposure to dromedary camels in Nigeria: an observational cohort study
    Article Snippet: A set of 20-mer peptides overlapping by ten amino acids encompassing the four MERS-CoV (HCoV-EMC/2012) structural proteins (peptides S1, S2, N, and ME encompassing the N-terminal and C-terminal portions of the spike [S] glycoprotein, the nucleocapsid [N] protein, and the transmembrane [M] and envelope [E] proteins) and five accessory proteins (ORF3, ORF4a, ORF4b, ORF5 and ORF8b) were synthesised by Sino Biological (Shanghai, China), and used for stimulation of PBMCs.

    Article Title: Identification and evaluation of potent Middle East respiratory syndrome coronavirus (MERS-CoV) 3CLPro inhibitors.
    Article Snippet: MERS-CoV NP was detected using a primary antibody specific for viral nucleocapsid protein (NP) (Cat. 100211-RP02; Sino Biological Inc., Beijing, China), followed by a horseradish peroxidase (HRP)-conjugated goat antirabbit secondary antibody (Thermo Scientific, Waltham, MA).

    Article Title: Identification and evaluation of potent Middle East respiratory syndrome coronavirus (MERS-CoV) 3CLPro inhibitors
    Article Snippet: MERS-CoV NP was detected using a primary antibody specific for viral nucleocapsid protein (NP) (Cat. 100211-RP02; Sino Biological Inc., Beijing, China), followed by a horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (Thermo Scientific, Waltham, MA).

    Article Title: mRNA-1273 efficacy in a severe COVID-19 model: attenuated activation of pulmonary immune cells after challenge
    Article Snippet: ELISAS, RBD or nucleocapsid proteins (1µg/mL, Sino Biological) were coated onto 96-well plates for 16 h. Plates were then blocked with SuperBlock (Pierce).

    Binding Assay:

    Article Title: Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platform
    Article Snippet: .. 2.1 MaterialsNucleocapsid protein (Nuc), the S1 fragment of the spike protein (S1), the extracellular domain of the spike protein (S1S2), the receptor binding domain of the spike protein (RBD), human serum albumin (HSA), the S1 domain of the 2005 SARS coronavirus spike protein (WH20 isolate, abbreviated “SARS-S1”), and human Influenza B nucleoprotein (B/Florida/4/2006 isolate “Flu Nuc”) were all obtained from Sino Biological, Inc. .. Positive control protein, human IgG protein (Hum IgG), SuperBlock blocking buffer and phosphate buffered saline (PBS) were obtained from ThermoFisher Scientific.

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  • 91
    Sino Biological human wisp 1 orf mammalian expression plasmid
    Osteogenic and adipogenic differentiation of human PSC with <t>WISP-1</t> knockdown. WISP-1 siRNA or scramble siRNA treated PSC were evaluated for osteogenic and adipogenic differentiation. ( A ) Knockdown efficiency of WISP-1 in human PSC, assessed by qRT-PCR. ( B – D ) Osteogenic differentiation of human PSC with or without WISP-1 knockdown. ( B ) Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 (Runt-related transcription factor 2), ALP (Alkaline Phosphatase), COL1A1 (Type I Collagen) , and OCN ( Osteocalcin ). ( C ) Alkaline phosphatase (ALP) staining and photometric quantification after 12 days of osteogenic differentiation with or without WISP-1 knockdown. ( D ) Bone nodule formation examined by Alizarin red (AR) staining after 12 days of osteogenic differentiation with or without WISP-1 knockdown. ( E ) Expression levels of markers of BMP and Wnt signaling activity by qRT-PCR at 3 days of osteogenic differentiation with or without WISP-1 knockdown, including ID1 (Inhibitor of DNA binding 1) and AXIN2 (Axis Inhibition Protein 2) . ( F ) PSC were treated with rBMP2 (50 ng/mL), with or without WISP-1 knockdown. Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 , ALP , and OCN . ( G ) Adipogenic differentiation of human PSC with or without WISP-1 knockdown. Adipocytic gene markers assessed by quantitative RT-PCR at 3 days of differentiation, including PPAR γ ( Peroxisome proliferator-activated receptor gamma ), CEBP α ( CCAAT/enhancer-binding protein alpha ), FABP4 (Fatty acid binding protein 4) , and LPL ( Lipoprotein lipase ). * P
    Human Wisp 1 Orf Mammalian Expression Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human wisp 1 orf mammalian expression plasmid/product/Sino Biological
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human wisp 1 orf mammalian expression plasmid - by Bioz Stars, 2021-02
    91/100 stars
      Buy from Supplier

    94
    Sino Biological nucleocapsid protein np
    Osteogenic and adipogenic differentiation of human PSC with <t>WISP-1</t> knockdown. WISP-1 siRNA or scramble siRNA treated PSC were evaluated for osteogenic and adipogenic differentiation. ( A ) Knockdown efficiency of WISP-1 in human PSC, assessed by qRT-PCR. ( B – D ) Osteogenic differentiation of human PSC with or without WISP-1 knockdown. ( B ) Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 (Runt-related transcription factor 2), ALP (Alkaline Phosphatase), COL1A1 (Type I Collagen) , and OCN ( Osteocalcin ). ( C ) Alkaline phosphatase (ALP) staining and photometric quantification after 12 days of osteogenic differentiation with or without WISP-1 knockdown. ( D ) Bone nodule formation examined by Alizarin red (AR) staining after 12 days of osteogenic differentiation with or without WISP-1 knockdown. ( E ) Expression levels of markers of BMP and Wnt signaling activity by qRT-PCR at 3 days of osteogenic differentiation with or without WISP-1 knockdown, including ID1 (Inhibitor of DNA binding 1) and AXIN2 (Axis Inhibition Protein 2) . ( F ) PSC were treated with rBMP2 (50 ng/mL), with or without WISP-1 knockdown. Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 , ALP , and OCN . ( G ) Adipogenic differentiation of human PSC with or without WISP-1 knockdown. Adipocytic gene markers assessed by quantitative RT-PCR at 3 days of differentiation, including PPAR γ ( Peroxisome proliferator-activated receptor gamma ), CEBP α ( CCAAT/enhancer-binding protein alpha ), FABP4 (Fatty acid binding protein 4) , and LPL ( Lipoprotein lipase ). * P
    Nucleocapsid Protein Np, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleocapsid protein np/product/Sino Biological
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nucleocapsid protein np - by Bioz Stars, 2021-02
    94/100 stars
      Buy from Supplier

    Image Search Results


    Osteogenic and adipogenic differentiation of human PSC with WISP-1 knockdown. WISP-1 siRNA or scramble siRNA treated PSC were evaluated for osteogenic and adipogenic differentiation. ( A ) Knockdown efficiency of WISP-1 in human PSC, assessed by qRT-PCR. ( B – D ) Osteogenic differentiation of human PSC with or without WISP-1 knockdown. ( B ) Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 (Runt-related transcription factor 2), ALP (Alkaline Phosphatase), COL1A1 (Type I Collagen) , and OCN ( Osteocalcin ). ( C ) Alkaline phosphatase (ALP) staining and photometric quantification after 12 days of osteogenic differentiation with or without WISP-1 knockdown. ( D ) Bone nodule formation examined by Alizarin red (AR) staining after 12 days of osteogenic differentiation with or without WISP-1 knockdown. ( E ) Expression levels of markers of BMP and Wnt signaling activity by qRT-PCR at 3 days of osteogenic differentiation with or without WISP-1 knockdown, including ID1 (Inhibitor of DNA binding 1) and AXIN2 (Axis Inhibition Protein 2) . ( F ) PSC were treated with rBMP2 (50 ng/mL), with or without WISP-1 knockdown. Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 , ALP , and OCN . ( G ) Adipogenic differentiation of human PSC with or without WISP-1 knockdown. Adipocytic gene markers assessed by quantitative RT-PCR at 3 days of differentiation, including PPAR γ ( Peroxisome proliferator-activated receptor gamma ), CEBP α ( CCAAT/enhancer-binding protein alpha ), FABP4 (Fatty acid binding protein 4) , and LPL ( Lipoprotein lipase ). * P

    Journal: Scientific Reports

    Article Title: WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells

    doi: 10.1038/s41598-018-34143-x

    Figure Lengend Snippet: Osteogenic and adipogenic differentiation of human PSC with WISP-1 knockdown. WISP-1 siRNA or scramble siRNA treated PSC were evaluated for osteogenic and adipogenic differentiation. ( A ) Knockdown efficiency of WISP-1 in human PSC, assessed by qRT-PCR. ( B – D ) Osteogenic differentiation of human PSC with or without WISP-1 knockdown. ( B ) Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 (Runt-related transcription factor 2), ALP (Alkaline Phosphatase), COL1A1 (Type I Collagen) , and OCN ( Osteocalcin ). ( C ) Alkaline phosphatase (ALP) staining and photometric quantification after 12 days of osteogenic differentiation with or without WISP-1 knockdown. ( D ) Bone nodule formation examined by Alizarin red (AR) staining after 12 days of osteogenic differentiation with or without WISP-1 knockdown. ( E ) Expression levels of markers of BMP and Wnt signaling activity by qRT-PCR at 3 days of osteogenic differentiation with or without WISP-1 knockdown, including ID1 (Inhibitor of DNA binding 1) and AXIN2 (Axis Inhibition Protein 2) . ( F ) PSC were treated with rBMP2 (50 ng/mL), with or without WISP-1 knockdown. Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 , ALP , and OCN . ( G ) Adipogenic differentiation of human PSC with or without WISP-1 knockdown. Adipocytic gene markers assessed by quantitative RT-PCR at 3 days of differentiation, including PPAR γ ( Peroxisome proliferator-activated receptor gamma ), CEBP α ( CCAAT/enhancer-binding protein alpha ), FABP4 (Fatty acid binding protein 4) , and LPL ( Lipoprotein lipase ). * P

    Article Snippet: WISP-1 overexpression WISP-1 overexpression was assayed using human WISP-1 ORF mammalian expression plasmid (HG10442, Sino Biological, North Wales, PA).

    Techniques: Quantitative RT-PCR, Expressing, Staining, Activity Assay, Binding Assay, Inhibition

    Osteogenic and adipogenic differentiation of human PSC with WISP-1 overexpression or rWISP-1 protein. ( A – D ) WISP-1 overexpression or control plasmid treated PSC were evaluated for osteogenic and adipogenic differentiation. ( A ) Efficacy of WISP-1 plasmid in human PSC, assessed by qRT-PCR at 6, 9, and 15 days. ( B ) Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 (Runt-related transcription factor 2), ALP (Alkaline Phosphatase), COL1A1 (Type I Collagen) , and OCN ( Osteocalcin ). ( C ) Adipocytic gene markers assessed by quantitative RT-PCR at 3 days of differentiation, including PPAR γ ( Peroxisome proliferator-activated receptor gamma ), CEBP α ( CCAAT/enhancer-binding protein alpha ). ( D ) Oil red O staining of human PSC with or without WISP-1 overexpression, 15 days of differentiation. ( E , F ) Osteogenic differentiation of human PSC with or without recombinant (r)WISP-1 protein (200 ng/mL). ( E ) Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 , ALP , and OCN . ( F ) Effects of rWISP-1 (200 ng/mL) and rBMP2 (50 ng/mL) combined application in human PSC. Expression levels of ALP assessed by qRT-PCR at 3 days of osteogenic differentiation. * P

    Journal: Scientific Reports

    Article Title: WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells

    doi: 10.1038/s41598-018-34143-x

    Figure Lengend Snippet: Osteogenic and adipogenic differentiation of human PSC with WISP-1 overexpression or rWISP-1 protein. ( A – D ) WISP-1 overexpression or control plasmid treated PSC were evaluated for osteogenic and adipogenic differentiation. ( A ) Efficacy of WISP-1 plasmid in human PSC, assessed by qRT-PCR at 6, 9, and 15 days. ( B ) Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 (Runt-related transcription factor 2), ALP (Alkaline Phosphatase), COL1A1 (Type I Collagen) , and OCN ( Osteocalcin ). ( C ) Adipocytic gene markers assessed by quantitative RT-PCR at 3 days of differentiation, including PPAR γ ( Peroxisome proliferator-activated receptor gamma ), CEBP α ( CCAAT/enhancer-binding protein alpha ). ( D ) Oil red O staining of human PSC with or without WISP-1 overexpression, 15 days of differentiation. ( E , F ) Osteogenic differentiation of human PSC with or without recombinant (r)WISP-1 protein (200 ng/mL). ( E ) Expression levels of osteogenic gene markers by qRT-PCR at 3 days of osteogenic differentiation, including RUNX2 , ALP , and OCN . ( F ) Effects of rWISP-1 (200 ng/mL) and rBMP2 (50 ng/mL) combined application in human PSC. Expression levels of ALP assessed by qRT-PCR at 3 days of osteogenic differentiation. * P

    Article Snippet: WISP-1 overexpression WISP-1 overexpression was assayed using human WISP-1 ORF mammalian expression plasmid (HG10442, Sino Biological, North Wales, PA).

    Techniques: Over Expression, Plasmid Preparation, Quantitative RT-PCR, Expressing, Binding Assay, Staining, Recombinant

    Immunohistochemical detection of WISP-1 protein in human adipose tissue. ( A) WISP-1 immunoreactivity within the vascular wall of small caliber vessels in cross section. ( B ) WISP-1 immunoreactivity within a venule (center) and branching capillaries in longitudinal cross section. ( C , D ) WISP-1 immunoreactivity in larger caliber veins. Scale bars = 25 μm.

    Journal: Scientific Reports

    Article Title: WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells

    doi: 10.1038/s41598-018-34143-x

    Figure Lengend Snippet: Immunohistochemical detection of WISP-1 protein in human adipose tissue. ( A) WISP-1 immunoreactivity within the vascular wall of small caliber vessels in cross section. ( B ) WISP-1 immunoreactivity within a venule (center) and branching capillaries in longitudinal cross section. ( C , D ) WISP-1 immunoreactivity in larger caliber veins. Scale bars = 25 μm.

    Article Snippet: WISP-1 overexpression WISP-1 overexpression was assayed using human WISP-1 ORF mammalian expression plasmid (HG10442, Sino Biological, North Wales, PA).

    Techniques: Immunohistochemistry

    WISP-1 expression in human PSC after FACS isolation and upon orthopaedic transplantation. ( A ) Fluorescence activated cell sorting (FACS) of human lipoaspirate was performed per established protocols to isolate perivascular stem/stromal cells (PSC). After isolation of CD31−CD45− non-endothelial/non-hematopoietic cells ( not shown ), PSC were further defined as adventitial progenitor cells (APC, CD34+CD146−) or microvascular pericytes (CD146+CD34−). ( B ) Quantitative RT-PCR for WISP1 transcripts, examined in passage one PSC or unpurified ASC (adipose-derived stromal cells) from the same patient sample. Performed in technical triplicate. ( C – F ) H E appearance of rat spine fusion segments. ( C , E ) H E appearance of PSC-treated spinal fusion segments with new bone formation, incorporation of demineralized bone matrix (DBM), and conspicuous bone-lining osteoblasts (arrowheads). ( D , F ) H E appearance of control-treated spinal fusion segments, with little new bone formation, DBM particles without connections to each other and embedded in fibrous stroma, and inactive bone lining cells (arrowheads). ( G – J ) WISP-1 indirect immunohistochemical staining of PSC treated spine fusion segments. WISP-1 immunoreactivity appears brown, while nuclear Hematoxylin counterstain appears blue. ( G , I ) Strong WISP-1 immunoreactivity in bone lining cells among PSC treated samples. ( H , J ) Weak to intermediate WISP-1 immunoreactivity in inflammatory cells and stromal fibroblasts among control treated samples. * P

    Journal: Scientific Reports

    Article Title: WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells

    doi: 10.1038/s41598-018-34143-x

    Figure Lengend Snippet: WISP-1 expression in human PSC after FACS isolation and upon orthopaedic transplantation. ( A ) Fluorescence activated cell sorting (FACS) of human lipoaspirate was performed per established protocols to isolate perivascular stem/stromal cells (PSC). After isolation of CD31−CD45− non-endothelial/non-hematopoietic cells ( not shown ), PSC were further defined as adventitial progenitor cells (APC, CD34+CD146−) or microvascular pericytes (CD146+CD34−). ( B ) Quantitative RT-PCR for WISP1 transcripts, examined in passage one PSC or unpurified ASC (adipose-derived stromal cells) from the same patient sample. Performed in technical triplicate. ( C – F ) H E appearance of rat spine fusion segments. ( C , E ) H E appearance of PSC-treated spinal fusion segments with new bone formation, incorporation of demineralized bone matrix (DBM), and conspicuous bone-lining osteoblasts (arrowheads). ( D , F ) H E appearance of control-treated spinal fusion segments, with little new bone formation, DBM particles without connections to each other and embedded in fibrous stroma, and inactive bone lining cells (arrowheads). ( G – J ) WISP-1 indirect immunohistochemical staining of PSC treated spine fusion segments. WISP-1 immunoreactivity appears brown, while nuclear Hematoxylin counterstain appears blue. ( G , I ) Strong WISP-1 immunoreactivity in bone lining cells among PSC treated samples. ( H , J ) Weak to intermediate WISP-1 immunoreactivity in inflammatory cells and stromal fibroblasts among control treated samples. * P

    Article Snippet: WISP-1 overexpression WISP-1 overexpression was assayed using human WISP-1 ORF mammalian expression plasmid (HG10442, Sino Biological, North Wales, PA).

    Techniques: Expressing, FACS, Isolation, Transplantation Assay, Fluorescence, Quantitative RT-PCR, Derivative Assay, Immunohistochemistry, Staining