h7n9  (Sino Biological)


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    Name:
    Influenza A H7N9 A Anhui 1 2013 Matrix protein 1 Codon Optimized Gene ORF cDNA clone expression plasmid N Flag tag
    Description:
    Full length Clone DNA of Influenza A H7N9 A Anhui 1 2013 Matrix protein 1 with N terminal Flag tag
    Catalog Number:
    vg40107-nf
    Price:
    295.0
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Category:
    cDNA Clone
    Molecule Name:
    M1,M1R,Matrix protein 1
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    Structured Review

    Sino Biological h7n9
    Influenza A H7N9 A Anhui 1 2013 Matrix protein 1 Codon Optimized Gene ORF cDNA clone expression plasmid N Flag tag
    Full length Clone DNA of Influenza A H7N9 A Anhui 1 2013 Matrix protein 1 with N terminal Flag tag
    https://www.bioz.com/result/h7n9/product/Sino Biological
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    h7n9 - by Bioz Stars, 2021-02
    93/100 stars

    Images

    1) Product Images from "Hemagglutinin-Specific Non-neutralizing Antibody Is Essential for Protection Provided by Inactivated and Viral-Vectored H7N9 Avian Influenza Vaccines in Chickens"

    Article Title: Hemagglutinin-Specific Non-neutralizing Antibody Is Essential for Protection Provided by Inactivated and Viral-Vectored H7N9 Avian Influenza Vaccines in Chickens

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2019.00482

    Antibody response induced by NDV-vectored H7N9 vaccine in chickens. (A) HI titers against NDV vector. (B) HI titers against H7N9 virus. The dotted lines in (A,B) indicate the cutoff value for positive reading in HI assay (4 log 2 ). (C) VN antibody titers against H7N9 virus. (D) IgG titers determined by ELISA. Serum samples from individual chicken were pooled for measurement of VN and IgG titers. The dotted lines in (C,D) stand for the lower detection limit for VN test and ELISA.
    Figure Legend Snippet: Antibody response induced by NDV-vectored H7N9 vaccine in chickens. (A) HI titers against NDV vector. (B) HI titers against H7N9 virus. The dotted lines in (A,B) indicate the cutoff value for positive reading in HI assay (4 log 2 ). (C) VN antibody titers against H7N9 virus. (D) IgG titers determined by ELISA. Serum samples from individual chicken were pooled for measurement of VN and IgG titers. The dotted lines in (C,D) stand for the lower detection limit for VN test and ELISA.

    Techniques Used: Plasmid Preparation, HI Assay, Enzyme-linked Immunosorbent Assay

    Passive transfer challenge study of non-neutralizing sera. (A) Schematic illustration for the serum transfer experiment. (B) Clinical scoring and (C) survival of chickens after H7N9 virus infection. “ns” in (C) indicates non-significant difference.
    Figure Legend Snippet: Passive transfer challenge study of non-neutralizing sera. (A) Schematic illustration for the serum transfer experiment. (B) Clinical scoring and (C) survival of chickens after H7N9 virus infection. “ns” in (C) indicates non-significant difference.

    Techniques Used: Infection

    Antibody response induced by inactivated H7N9 vaccine in chickens. (A) HI, (B) VN, and (C) IgG titers induced by the vaccine. (D) Correlation analysis between VN and HI antibody titers. (E) Correlation analysis between IgG and HI antibody titers. (F) Correlation analysis between IgG and VN antibody titers. The dotted line in (A) indicates the cutoff value for positive reading in HI assay (4 log 2 ) and the lines in (B,C) stand for the lower detection limit for serological assays. GMT in (D–F) stands for geometry mean titer.
    Figure Legend Snippet: Antibody response induced by inactivated H7N9 vaccine in chickens. (A) HI, (B) VN, and (C) IgG titers induced by the vaccine. (D) Correlation analysis between VN and HI antibody titers. (E) Correlation analysis between IgG and HI antibody titers. (F) Correlation analysis between IgG and VN antibody titers. The dotted line in (A) indicates the cutoff value for positive reading in HI assay (4 log 2 ) and the lines in (B,C) stand for the lower detection limit for serological assays. GMT in (D–F) stands for geometry mean titer.

    Techniques Used: HI Assay

    Clinical scoring and survival of animals after H7N9 challenge. (A) Clinical scoring and (B) survival of chickens immunized with the rGD15 vaccine. (C) Clinical scoring and (D) survival of chickens immunized with the NDV-H7N9 vaccine.
    Figure Legend Snippet: Clinical scoring and survival of animals after H7N9 challenge. (A) Clinical scoring and (B) survival of chickens immunized with the rGD15 vaccine. (C) Clinical scoring and (D) survival of chickens immunized with the NDV-H7N9 vaccine.

    Techniques Used:

    2) Product Images from "Isolation of Recombinant Phage Antibodies Targeting the Hemagglutinin Cleavage Site of Highly Pathogenic Avian Influenza Virus"

    Article Title: Isolation of Recombinant Phage Antibodies Targeting the Hemagglutinin Cleavage Site of Highly Pathogenic Avian Influenza Virus

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0061158

    Binding specificity of the clones. (A) ELISA to determine binding to several other HA proteins. H5N1-HA(An): A/Anhui/1/05(H5N1) HA; H5N1-HA(Tu): A/Turkey/1/2005(H5N1) HA; H7N7-HA(Ne): A/Netherlands/219/03(H7N7) HA. (B) ELISA to determine epitope sequence. The inhibitory effect of 7-mer partial peptide sequences shown on the binding of Fab-phages to SAv-HA331 peptide was investigated. The peptide NSPQRER showed maximal inhibitory effect. (C) The cleavage site peptide sequence of HA proteins used. The presumptive peripheral and core epitope sequences are shown in brown and red, respectively. H6 is shown as a reference.
    Figure Legend Snippet: Binding specificity of the clones. (A) ELISA to determine binding to several other HA proteins. H5N1-HA(An): A/Anhui/1/05(H5N1) HA; H5N1-HA(Tu): A/Turkey/1/2005(H5N1) HA; H7N7-HA(Ne): A/Netherlands/219/03(H7N7) HA. (B) ELISA to determine epitope sequence. The inhibitory effect of 7-mer partial peptide sequences shown on the binding of Fab-phages to SAv-HA331 peptide was investigated. The peptide NSPQRER showed maximal inhibitory effect. (C) The cleavage site peptide sequence of HA proteins used. The presumptive peripheral and core epitope sequences are shown in brown and red, respectively. H6 is shown as a reference.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Sequencing

    3) Product Images from "A potent germline-like human monoclonal antibody targets a pH-sensitive epitope on H7N9 influenza hemagglutinin"

    Article Title: A potent germline-like human monoclonal antibody targets a pH-sensitive epitope on H7N9 influenza hemagglutinin

    Journal: Cell host & microbe

    doi: 10.1016/j.chom.2017.08.011

    m826 mAb recognizes a unique epitope on H7 HA. (A ) Docking of the m826-HA1 complex onto the crystal structure of monomeric H7 HA. The epitope of m826 is distinct from the RBS. (B) Superposition of the antibody epitope which was partially buried in the trimeric HA structure. (C) Sequence alignment of HA1 from the H7N9, H7N7, and H7N4 and highlighting of structurally defined influenza antibody epitopes. The conventional antigenic sites (Sa, Sb, Ca1, Ca2, and Cb) are shaded in blue, the epitope of m826 is shaded in red, and RBS is shown in the black box.
    Figure Legend Snippet: m826 mAb recognizes a unique epitope on H7 HA. (A ) Docking of the m826-HA1 complex onto the crystal structure of monomeric H7 HA. The epitope of m826 is distinct from the RBS. (B) Superposition of the antibody epitope which was partially buried in the trimeric HA structure. (C) Sequence alignment of HA1 from the H7N9, H7N7, and H7N4 and highlighting of structurally defined influenza antibody epitopes. The conventional antigenic sites (Sa, Sb, Ca1, Ca2, and Cb) are shaded in blue, the epitope of m826 is shaded in red, and RBS is shown in the black box.

    Techniques Used: Sequencing

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Generation and Characterization of Monoclonal Antibodies Specific to Avian Influenza H5N1 Hemagglutinin Protein.
    Article Snippet: .. Highly pathogenic avian influenza (HPAI) H5N1 virus has in the past breached the species barrier from infected domestic poultry to humans in close contact. .. Highly pathogenic avian influenza (HPAI) H5N1 virus has in the past breached the species barrier from infected domestic poultry to humans in close contact.

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  • 93
    Sino Biological h7n9
    Antibody response induced by NDV-vectored <t>H7N9</t> vaccine in chickens. (A) HI titers against NDV vector. (B) HI titers against H7N9 virus. The dotted lines in (A,B) indicate the cutoff value for positive reading in HI assay (4 log 2 ). (C) VN antibody titers against H7N9 virus. (D) IgG titers determined by ELISA. Serum samples from individual chicken were pooled for measurement of VN and IgG titers. The dotted lines in (C,D) stand for the lower detection limit for VN test and ELISA.
    H7n9, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h7n9/product/Sino Biological
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    h7n9 - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

    90
    Sino Biological h7n7
    Binding specificity of the clones. (A) ELISA to determine binding to several other HA proteins. H5N1-HA(An): A/Anhui/1/05(H5N1) HA; H5N1-HA(Tu): A/Turkey/1/2005(H5N1) HA; <t>H7N7-HA(Ne):</t> A/Netherlands/219/03(H7N7) HA. (B) ELISA to determine epitope sequence. The inhibitory effect of 7-mer partial peptide sequences shown on the binding of Fab-phages to SAv-HA331 peptide was investigated. The peptide NSPQRER showed maximal inhibitory effect. (C) The cleavage site peptide sequence of HA proteins used. The presumptive peripheral and core epitope sequences are shown in brown and red, respectively. H6 is shown as a reference.
    H7n7, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h7n7/product/Sino Biological
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    h7n7 - by Bioz Stars, 2021-02
    90/100 stars
      Buy from Supplier

    N/A
    Full length Clone DNA of Influenza B Brisbane 60 2008 Hemagglutinin with C terminal His tag
      Buy from Supplier

    Image Search Results


    Antibody response induced by NDV-vectored H7N9 vaccine in chickens. (A) HI titers against NDV vector. (B) HI titers against H7N9 virus. The dotted lines in (A,B) indicate the cutoff value for positive reading in HI assay (4 log 2 ). (C) VN antibody titers against H7N9 virus. (D) IgG titers determined by ELISA. Serum samples from individual chicken were pooled for measurement of VN and IgG titers. The dotted lines in (C,D) stand for the lower detection limit for VN test and ELISA.

    Journal: Frontiers in Veterinary Science

    Article Title: Hemagglutinin-Specific Non-neutralizing Antibody Is Essential for Protection Provided by Inactivated and Viral-Vectored H7N9 Avian Influenza Vaccines in Chickens

    doi: 10.3389/fvets.2019.00482

    Figure Lengend Snippet: Antibody response induced by NDV-vectored H7N9 vaccine in chickens. (A) HI titers against NDV vector. (B) HI titers against H7N9 virus. The dotted lines in (A,B) indicate the cutoff value for positive reading in HI assay (4 log 2 ). (C) VN antibody titers against H7N9 virus. (D) IgG titers determined by ELISA. Serum samples from individual chicken were pooled for measurement of VN and IgG titers. The dotted lines in (C,D) stand for the lower detection limit for VN test and ELISA.

    Article Snippet: The HA and HA1 (mainly the globular head region) proteins of H7N9 (A/Anhui/2/2013, AH13) were purchased from Sino Biological (Sino Biological, Beijing, China).

    Techniques: Plasmid Preparation, HI Assay, Enzyme-linked Immunosorbent Assay

    Passive transfer challenge study of non-neutralizing sera. (A) Schematic illustration for the serum transfer experiment. (B) Clinical scoring and (C) survival of chickens after H7N9 virus infection. “ns” in (C) indicates non-significant difference.

    Journal: Frontiers in Veterinary Science

    Article Title: Hemagglutinin-Specific Non-neutralizing Antibody Is Essential for Protection Provided by Inactivated and Viral-Vectored H7N9 Avian Influenza Vaccines in Chickens

    doi: 10.3389/fvets.2019.00482

    Figure Lengend Snippet: Passive transfer challenge study of non-neutralizing sera. (A) Schematic illustration for the serum transfer experiment. (B) Clinical scoring and (C) survival of chickens after H7N9 virus infection. “ns” in (C) indicates non-significant difference.

    Article Snippet: The HA and HA1 (mainly the globular head region) proteins of H7N9 (A/Anhui/2/2013, AH13) were purchased from Sino Biological (Sino Biological, Beijing, China).

    Techniques: Infection

    Antibody response induced by inactivated H7N9 vaccine in chickens. (A) HI, (B) VN, and (C) IgG titers induced by the vaccine. (D) Correlation analysis between VN and HI antibody titers. (E) Correlation analysis between IgG and HI antibody titers. (F) Correlation analysis between IgG and VN antibody titers. The dotted line in (A) indicates the cutoff value for positive reading in HI assay (4 log 2 ) and the lines in (B,C) stand for the lower detection limit for serological assays. GMT in (D–F) stands for geometry mean titer.

    Journal: Frontiers in Veterinary Science

    Article Title: Hemagglutinin-Specific Non-neutralizing Antibody Is Essential for Protection Provided by Inactivated and Viral-Vectored H7N9 Avian Influenza Vaccines in Chickens

    doi: 10.3389/fvets.2019.00482

    Figure Lengend Snippet: Antibody response induced by inactivated H7N9 vaccine in chickens. (A) HI, (B) VN, and (C) IgG titers induced by the vaccine. (D) Correlation analysis between VN and HI antibody titers. (E) Correlation analysis between IgG and HI antibody titers. (F) Correlation analysis between IgG and VN antibody titers. The dotted line in (A) indicates the cutoff value for positive reading in HI assay (4 log 2 ) and the lines in (B,C) stand for the lower detection limit for serological assays. GMT in (D–F) stands for geometry mean titer.

    Article Snippet: The HA and HA1 (mainly the globular head region) proteins of H7N9 (A/Anhui/2/2013, AH13) were purchased from Sino Biological (Sino Biological, Beijing, China).

    Techniques: HI Assay

    Clinical scoring and survival of animals after H7N9 challenge. (A) Clinical scoring and (B) survival of chickens immunized with the rGD15 vaccine. (C) Clinical scoring and (D) survival of chickens immunized with the NDV-H7N9 vaccine.

    Journal: Frontiers in Veterinary Science

    Article Title: Hemagglutinin-Specific Non-neutralizing Antibody Is Essential for Protection Provided by Inactivated and Viral-Vectored H7N9 Avian Influenza Vaccines in Chickens

    doi: 10.3389/fvets.2019.00482

    Figure Lengend Snippet: Clinical scoring and survival of animals after H7N9 challenge. (A) Clinical scoring and (B) survival of chickens immunized with the rGD15 vaccine. (C) Clinical scoring and (D) survival of chickens immunized with the NDV-H7N9 vaccine.

    Article Snippet: The HA and HA1 (mainly the globular head region) proteins of H7N9 (A/Anhui/2/2013, AH13) were purchased from Sino Biological (Sino Biological, Beijing, China).

    Techniques:

    Binding specificity of the clones. (A) ELISA to determine binding to several other HA proteins. H5N1-HA(An): A/Anhui/1/05(H5N1) HA; H5N1-HA(Tu): A/Turkey/1/2005(H5N1) HA; H7N7-HA(Ne): A/Netherlands/219/03(H7N7) HA. (B) ELISA to determine epitope sequence. The inhibitory effect of 7-mer partial peptide sequences shown on the binding of Fab-phages to SAv-HA331 peptide was investigated. The peptide NSPQRER showed maximal inhibitory effect. (C) The cleavage site peptide sequence of HA proteins used. The presumptive peripheral and core epitope sequences are shown in brown and red, respectively. H6 is shown as a reference.

    Journal: PLoS ONE

    Article Title: Isolation of Recombinant Phage Antibodies Targeting the Hemagglutinin Cleavage Site of Highly Pathogenic Avian Influenza Virus

    doi: 10.1371/journal.pone.0061158

    Figure Lengend Snippet: Binding specificity of the clones. (A) ELISA to determine binding to several other HA proteins. H5N1-HA(An): A/Anhui/1/05(H5N1) HA; H5N1-HA(Tu): A/Turkey/1/2005(H5N1) HA; H7N7-HA(Ne): A/Netherlands/219/03(H7N7) HA. (B) ELISA to determine epitope sequence. The inhibitory effect of 7-mer partial peptide sequences shown on the binding of Fab-phages to SAv-HA331 peptide was investigated. The peptide NSPQRER showed maximal inhibitory effect. (C) The cleavage site peptide sequence of HA proteins used. The presumptive peripheral and core epitope sequences are shown in brown and red, respectively. H6 is shown as a reference.

    Article Snippet: Recombinant HA proteins derived from influenza A virus H1N1 (A/California/04/09), H5N1 (A/Vietnam/1194/04, A/Anhui/1/05, and A/Turkey/1/05) and H7N7 (A/Netherlands/219/03), and HA-Fc fusion protein (A/Vietnam/1194/2004(H5N1), in which the cleavage site RERRRKKR was mutated to TETR) were purchased from Sino Biological Inc. (Beijing, China), as well as a mouse monoclonal antibody against H5N1.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Sequencing