Article Title: Expanding the epitranscriptomic RNA sequencing and modification mapping mass spectrometry toolbox with field asymmetric waveform ion mobility and electrochemical elution liquid chromatography
Figure Lengend Snippet: A)Ion Inject times of RNase T1 treated tRNA in different CV values in direct injection. B) Coverage of tRNA genes identified by FAIMS in triplicate technical replicates with unique spectra per gene identified by color bar. C) Direct Injection FAIMS comparing spectra sequenced from RNase A and RNase T1 digestion treatments. D) Distributions of sequenced RNA lengths between FAIMS CV and RNase treatment. E) Overlapping identifications of RNase treatment with RNase A (red) and RNase T1 (blue) showing individual sequence coverage across all tRNAs.
Article Snippet: Direct Injection RNA FAIMS The E coli and S. cerevisiae tRNA standard was purchased from Thermo Fisher and digested with both RNase T1 and RNase A (#T3018) from NEB biosciences for 45 minutes at room temperature in triplicate.
Techniques: Injection, Sequencing