pbstw  (New England Biolabs)


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  • 96
    Name:
    Monarch RNase A
    Description:
    Monarch RNase A is a component of the Monarch Genomic DNA Purification Kit Treatment with RNase A is an optional step in the protocol to remove any residual RNA present This high quality enzyme preparation is purified from bovine pancreas and is DNase protease and DNA free It is supplied as a 20 mg ml solution in 50 glycerol 50 mM Tris Cl pH 7 4
    Catalog Number:
    T3018L
    Price:
    62
    Category:
    Genomic DNA Purification Kit Components
    Size:
    1 ml
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    New England Biolabs pbstw
    Monarch RNase A
    Monarch RNase A is a component of the Monarch Genomic DNA Purification Kit Treatment with RNase A is an optional step in the protocol to remove any residual RNA present This high quality enzyme preparation is purified from bovine pancreas and is DNase protease and DNA free It is supplied as a 20 mg ml solution in 50 glycerol 50 mM Tris Cl pH 7 4
    https://www.bioz.com/result/pbstw/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbstw - by Bioz Stars, 2021-05
    96/100 stars

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    New England Biolabs monarch rnase a
    The use of <t>RNase</t> A as a proxy for viable virus versus non-viable virus. RNase should not penetrate the nuclease resistant viral envelope and only digest free circulating RNA. After purification of the RNase digested samples (37°C, 30 minutes), samples are eluted in the qPCR RNase Blocker. Gamma irradiated virus (Red) is less digestable than pure genomic RNA (Purple). Viral gDNA remains resistant to RNase digestion (Blue). Gamma irradiated virus spiked onto cannabis flower is fully digestable (Green) by RNase suggesting non-infectious virus by Day 5 at Room Temperature storage. Non-irradiated virus is expected to last much longer on cannabis.
    Monarch Rnase A, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rnase a/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rnase a - by Bioz Stars, 2021-05
    96/100 stars
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    The use of RNase A as a proxy for viable virus versus non-viable virus. RNase should not penetrate the nuclease resistant viral envelope and only digest free circulating RNA. After purification of the RNase digested samples (37°C, 30 minutes), samples are eluted in the qPCR RNase Blocker. Gamma irradiated virus (Red) is less digestable than pure genomic RNA (Purple). Viral gDNA remains resistant to RNase digestion (Blue). Gamma irradiated virus spiked onto cannabis flower is fully digestable (Green) by RNase suggesting non-infectious virus by Day 5 at Room Temperature storage. Non-irradiated virus is expected to last much longer on cannabis.

    Journal: bioRxiv

    Article Title: Quantitative PCR for cannabis flower containing SARs-CoV-2

    doi: 10.1101/2020.06.06.112474

    Figure Lengend Snippet: The use of RNase A as a proxy for viable virus versus non-viable virus. RNase should not penetrate the nuclease resistant viral envelope and only digest free circulating RNA. After purification of the RNase digested samples (37°C, 30 minutes), samples are eluted in the qPCR RNase Blocker. Gamma irradiated virus (Red) is less digestable than pure genomic RNA (Purple). Viral gDNA remains resistant to RNase digestion (Blue). Gamma irradiated virus spiked onto cannabis flower is fully digestable (Green) by RNase suggesting non-infectious virus by Day 5 at Room Temperature storage. Non-irradiated virus is expected to last much longer on cannabis.

    Article Snippet: 4 μL of 20 mg/mL RNase A (New England Biolabs #T3018L) is added to the RNase positive aliquot of non-lysed TSB from 1-gram cannabis flower homogenate.

    Techniques: Purification, Real-time Polymerase Chain Reaction, Irradiation