monarch gdna elution buffer  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Monarch gDNA Elution Buffer
    Description:
    Monarch gDNA Elution Buffer is the elution buffer included in the Monarch Genomic DNA Purification Kit It is supplied in 10 mM Tris HCl pH 9 0 0 1 mM EDTA and is suitable for long term storage of gDNA Nucleases are inactivated both by the inclusion of EDTA and the pH of the solution
    Catalog Number:
    T3016L
    Price:
    32
    Category:
    Genomic DNA Purification Kit Components
    Size:
    34 ml
    Buy from Supplier


    Structured Review

    New England Biolabs monarch gdna elution buffer
    Monarch gDNA Elution Buffer
    Monarch gDNA Elution Buffer is the elution buffer included in the Monarch Genomic DNA Purification Kit It is supplied in 10 mM Tris HCl pH 9 0 0 1 mM EDTA and is suitable for long term storage of gDNA Nucleases are inactivated both by the inclusion of EDTA and the pH of the solution
    https://www.bioz.com/result/monarch gdna elution buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna elution buffer - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "Erosion of the Epigenetic Landscape and Loss of Cellular Identity as a Cause of Aging in Mammals"

    Article Title: Erosion of the Epigenetic Landscape and Loss of Cellular Identity as a Cause of Aging in Mammals

    Journal: bioRxiv

    doi: 10.1101/808642

    A Cell-based System to Study the Effect of DSBs on the Epigenome, Related to Figure 1 (A) RNA-seq volcano plots (Cre vs. ICE) of cells from the same or different litters before 4-OHT treatment. (B) Slot blots to compare global 5mC levels. Methylene blue (MB) staining showed total genomic DNA used in each sample. (C) Genomic distribution of I- Ppo I canonical sites and all 90 CpG sites of the mouse epigenetic clock. (D) Percent non-mutated sequences of ∼100,000 random sites in post-treated ICE cells assessed by deep sequencing ( > 50x). (E and F) Immunostaining of DNA damage markers γH2AX and 53BP1 in post-treated ICE cells with and without exposure to the DNA damaging agents (ETS, etoposide; CPT, camptothecin; H 2 O 2 , hydrogen peroxide). Two-tailed Student’s t test. Scale bar, 10 µm. (G) Immunostaining of Lamin B1 in post-treated ICE cells. Data are mean (n=3) ± SD. *p
    Figure Legend Snippet: A Cell-based System to Study the Effect of DSBs on the Epigenome, Related to Figure 1 (A) RNA-seq volcano plots (Cre vs. ICE) of cells from the same or different litters before 4-OHT treatment. (B) Slot blots to compare global 5mC levels. Methylene blue (MB) staining showed total genomic DNA used in each sample. (C) Genomic distribution of I- Ppo I canonical sites and all 90 CpG sites of the mouse epigenetic clock. (D) Percent non-mutated sequences of ∼100,000 random sites in post-treated ICE cells assessed by deep sequencing ( > 50x). (E and F) Immunostaining of DNA damage markers γH2AX and 53BP1 in post-treated ICE cells with and without exposure to the DNA damaging agents (ETS, etoposide; CPT, camptothecin; H 2 O 2 , hydrogen peroxide). Two-tailed Student’s t test. Scale bar, 10 µm. (G) Immunostaining of Lamin B1 in post-treated ICE cells. Data are mean (n=3) ± SD. *p

    Techniques Used: RNA Sequencing Assay, Staining, Sequencing, Immunostaining, Two Tailed Test

    Related Articles

    other:

    Article Title: Phenotypic changes of bacteria through opportunity and global methylation leads to antibiotic resistance
    Article Snippet: Library mix was cleaned up using 50 µl of myOne C1 beads and finally eluted in 25 µl of elution buffer.

    Chromatin Immunoprecipitation:

    Article Title: Erosion of the Epigenetic Landscape and Loss of Cellular Identity as a Cause of Aging in Mammals
    Article Snippet: .. ChIP DNA was eluted by incubating at 65°C overnight in elution buffer containing 0.25% SDS, 1 mM EDTA, 10 mM Tris-HCl pH7.5. .. ChIP DNA was eluted by incubating at 65°C overnight in elution buffer containing 0.25% SDS, 1 mM EDTA, 10 mM Tris-HCl pH7.5.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs monarch gdna elution buffer
    A Cell-based System to Study the Effect of DSBs on the Epigenome, Related to Figure 1 (A) RNA-seq volcano plots (Cre vs. ICE) of cells from the same or different litters before 4-OHT treatment. (B) Slot blots to compare global 5mC levels. Methylene blue (MB) staining showed total genomic <t>DNA</t> used in each sample. (C) Genomic distribution of I- Ppo I canonical sites and all 90 CpG sites of the mouse epigenetic clock. (D) Percent non-mutated sequences of ∼100,000 random sites in post-treated ICE cells assessed by deep sequencing ( > 50x). (E and F) Immunostaining of DNA damage markers γH2AX and 53BP1 in post-treated ICE cells with and without exposure to the DNA damaging agents (ETS, etoposide; CPT, camptothecin; H 2 O 2 , hydrogen peroxide). Two-tailed Student’s t test. Scale bar, 10 µm. (G) Immunostaining of Lamin B1 in post-treated ICE cells. Data are mean (n=3) ± SD. *p
    Monarch Gdna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch gdna elution buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna elution buffer - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    A Cell-based System to Study the Effect of DSBs on the Epigenome, Related to Figure 1 (A) RNA-seq volcano plots (Cre vs. ICE) of cells from the same or different litters before 4-OHT treatment. (B) Slot blots to compare global 5mC levels. Methylene blue (MB) staining showed total genomic DNA used in each sample. (C) Genomic distribution of I- Ppo I canonical sites and all 90 CpG sites of the mouse epigenetic clock. (D) Percent non-mutated sequences of ∼100,000 random sites in post-treated ICE cells assessed by deep sequencing ( > 50x). (E and F) Immunostaining of DNA damage markers γH2AX and 53BP1 in post-treated ICE cells with and without exposure to the DNA damaging agents (ETS, etoposide; CPT, camptothecin; H 2 O 2 , hydrogen peroxide). Two-tailed Student’s t test. Scale bar, 10 µm. (G) Immunostaining of Lamin B1 in post-treated ICE cells. Data are mean (n=3) ± SD. *p

    Journal: bioRxiv

    Article Title: Erosion of the Epigenetic Landscape and Loss of Cellular Identity as a Cause of Aging in Mammals

    doi: 10.1101/808642

    Figure Lengend Snippet: A Cell-based System to Study the Effect of DSBs on the Epigenome, Related to Figure 1 (A) RNA-seq volcano plots (Cre vs. ICE) of cells from the same or different litters before 4-OHT treatment. (B) Slot blots to compare global 5mC levels. Methylene blue (MB) staining showed total genomic DNA used in each sample. (C) Genomic distribution of I- Ppo I canonical sites and all 90 CpG sites of the mouse epigenetic clock. (D) Percent non-mutated sequences of ∼100,000 random sites in post-treated ICE cells assessed by deep sequencing ( > 50x). (E and F) Immunostaining of DNA damage markers γH2AX and 53BP1 in post-treated ICE cells with and without exposure to the DNA damaging agents (ETS, etoposide; CPT, camptothecin; H 2 O 2 , hydrogen peroxide). Two-tailed Student’s t test. Scale bar, 10 µm. (G) Immunostaining of Lamin B1 in post-treated ICE cells. Data are mean (n=3) ± SD. *p

    Article Snippet: ChIP DNA was eluted by incubating at 65°C overnight in elution buffer containing 0.25% SDS, 1 mM EDTA, 10 mM Tris-HCl pH7.5.

    Techniques: RNA Sequencing Assay, Staining, Sequencing, Immunostaining, Two Tailed Test