monarch gdna wash buffer  (New England Biolabs)


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    Name:
    Monarch gDNA Wash Buffer
    Description:
    Monarch gDNA Wash Buffer is a component of the Monarch Genomic DNA Purification Kit It is an ethanol based low salt buffer that allows for complete removal of chaotropic salt and cell components in just 2 wash steps enabling the purification of highly pure gDNA
    Catalog Number:
    T3015L
    Price:
    32
    Category:
    Genomic DNA Purification Kit Components
    Size:
    60 ml
    Buy from Supplier


    Structured Review

    New England Biolabs monarch gdna wash buffer
    Monarch gDNA Wash Buffer
    Monarch gDNA Wash Buffer is a component of the Monarch Genomic DNA Purification Kit It is an ethanol based low salt buffer that allows for complete removal of chaotropic salt and cell components in just 2 wash steps enabling the purification of highly pure gDNA
    https://www.bioz.com/result/monarch gdna wash buffer/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna wash buffer - by Bioz Stars, 2021-05
    96/100 stars

    Images

    1) Product Images from "Dissecting individual pathogen-commensal interactions within a complex gut microbiota community"

    Article Title: Dissecting individual pathogen-commensal interactions within a complex gut microbiota community

    Journal: bioRxiv

    doi: 10.1101/2020.04.06.027128

    Schematic diagram of the pipeline used to quantify individual species in a mixed biofilm. Samples are PMA (propidium monoazide) treated to ensure quantification of genomic DNA only from live cells. After treatment, cells are lysed, DNA is extracted, followed by qPCR quantification of DNA and subsequent conversion to bacterial numbers.
    Figure Legend Snippet: Schematic diagram of the pipeline used to quantify individual species in a mixed biofilm. Samples are PMA (propidium monoazide) treated to ensure quantification of genomic DNA only from live cells. After treatment, cells are lysed, DNA is extracted, followed by qPCR quantification of DNA and subsequent conversion to bacterial numbers.

    Techniques Used: Real-time Polymerase Chain Reaction

    2) Product Images from "Detection of CRISPR-Cas9-Mediated Mutations Using a Carbon Nanotube-Modified Electrochemical Genosensor"

    Article Title: Detection of CRISPR-Cas9-Mediated Mutations Using a Carbon Nanotube-Modified Electrochemical Genosensor

    Journal: Biosensors

    doi: 10.3390/bios11010017

    Clustered regularly interspaced short palindromic repeats (CRISPR) RNA (crRNA) was designed with complementary nucleotides adjacent to the protospacer adjacent motif (PAM) to target CRISPR-associated protein 9 (Cas9) endonuclease to the correct DNA sequence within genomic DNA. HNH (an endonuclease domain named for characteristic histidine and asparagine residues) and RuvC-like nuclease domains cut the target DNA 3 nucleotides upstream of the PAM (NGG) sequence leading to the formation of a double-strand break. Mutations were inserted utilizing a homology-directed repair (HDR) pathway to repair the dsDNA breaks using 98 nt single-strand oligodeoxynucleotide (ODN) sequence as a template with 40–50 nucleotides flanking the dsDNA break site to introduce point mutations (PM).
    Figure Legend Snippet: Clustered regularly interspaced short palindromic repeats (CRISPR) RNA (crRNA) was designed with complementary nucleotides adjacent to the protospacer adjacent motif (PAM) to target CRISPR-associated protein 9 (Cas9) endonuclease to the correct DNA sequence within genomic DNA. HNH (an endonuclease domain named for characteristic histidine and asparagine residues) and RuvC-like nuclease domains cut the target DNA 3 nucleotides upstream of the PAM (NGG) sequence leading to the formation of a double-strand break. Mutations were inserted utilizing a homology-directed repair (HDR) pathway to repair the dsDNA breaks using 98 nt single-strand oligodeoxynucleotide (ODN) sequence as a template with 40–50 nucleotides flanking the dsDNA break site to introduce point mutations (PM).

    Techniques Used: CRISPR, Sequencing, Introduce

    Related Articles

    Sampling:

    Article Title: Whole mitochondrial genome sequence and phylogenetic relationships of Williams’s jerboa (Scarturus williamsi) from Turkey
    Article Snippet: To rectify these limitations, the present study aimed to (i) generate and assemble the first mitogenome data of Turkish S. williamsi by using the next-generation sequencing platform, (ii) characterize the mitogenome of the species by comparing it with the available mitogenomes of other jerboa species, and (iii) reveal the phylogenetic relationships of S. williamsi based on previously published and available mitogenome data in GenBank of Dipodoidea/Myomorpha species. .. Sampling, gDNA extraction and long-range PCR amplification A tissue sample (muscle) was obtained from one female individual of S. williamsi collected from Yeşilli Village, Sulakyurt, Kırıkkale (road-killed individual; collection number: 484). .. Genomic DNA (gDNA) isolation was conducted using the QIAGEN DNeasy® Blood & Tissue Kit by following the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Whole mitochondrial genome sequence and phylogenetic relationships of Williams’s jerboa (Scarturus williamsi) from Turkey
    Article Snippet: To rectify these limitations, the present study aimed to (i) generate and assemble the first mitogenome data of Turkish S. williamsi by using the next-generation sequencing platform, (ii) characterize the mitogenome of the species by comparing it with the available mitogenomes of other jerboa species, and (iii) reveal the phylogenetic relationships of S. williamsi based on previously published and available mitogenome data in GenBank of Dipodoidea/Myomorpha species. .. Sampling, gDNA extraction and long-range PCR amplification A tissue sample (muscle) was obtained from one female individual of S. williamsi collected from Yeşilli Village, Sulakyurt, Kırıkkale (road-killed individual; collection number: 484). .. Genomic DNA (gDNA) isolation was conducted using the QIAGEN DNeasy® Blood & Tissue Kit by following the manufacturer’s instructions.

    Amplification:

    Article Title: Whole mitochondrial genome sequence and phylogenetic relationships of Williams’s jerboa (Scarturus williamsi) from Turkey
    Article Snippet: To rectify these limitations, the present study aimed to (i) generate and assemble the first mitogenome data of Turkish S. williamsi by using the next-generation sequencing platform, (ii) characterize the mitogenome of the species by comparing it with the available mitogenomes of other jerboa species, and (iii) reveal the phylogenetic relationships of S. williamsi based on previously published and available mitogenome data in GenBank of Dipodoidea/Myomorpha species. .. Sampling, gDNA extraction and long-range PCR amplification A tissue sample (muscle) was obtained from one female individual of S. williamsi collected from Yeşilli Village, Sulakyurt, Kırıkkale (road-killed individual; collection number: 484). .. Genomic DNA (gDNA) isolation was conducted using the QIAGEN DNeasy® Blood & Tissue Kit by following the manufacturer’s instructions.

    DNA Extraction:

    Article Title: Dissecting individual pathogen-commensal interactions within a complex gut microbiota community
    Article Snippet: .. Genomic DNA extraction DNA extraction was carried out using a phenol chloroform-based method. .. The cell pellets were re-suspended in 500 μl of 5 mg/ml lysozyme (VWR) and incubated for 20 min at 37°C.

    Article Title: Detection of CRISPR-Cas9-Mediated Mutations Using a Carbon Nanotube-Modified Electrochemical Genosensor
    Article Snippet: Single cells were cultured in a flat bottomed 96-well plate for 2 weeks, replacing media every 2–3 days and passaging cells on to 24 well plates. .. Genomic DNA (gDNA) was extracted using 50 μL of QuickExtract™ DNA extraction solution (Epicentre-Lucigen). .. The sequences of interest were screened after digestion with BanI (S331) restriction enzymes. gDNA was PCR amplified using One Taq Quick-Load 2× master mix (New England BioLabs, Ipswich, MA, USA), 94 °C-30 s, 30 cycles of 94 °C-30 s, 68 °C-30 s, 68 °C-60 s, the final extension was carried out at of 68 °C-5 min. PCR products were DNA sequenced to verify the presence of mutations.

    Purification:

    Article Title: The receptor PTPRU is a redox sensitive pseudophosphatase
    Article Snippet: Recombinant protein pull downs50 μg of biotinylated Avi-tag recombinant PTP domains were bound to 167 μl of streptavidin-coated magnetic beads (New England Biolabs) made up to a total volume of 500 μl in ice-cold purification buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 5% [v/v] glycerol, 5 mM DTT) at 4 °C for 1.5 h with rotation. .. Beads were collected using a magnetic stand and washed 3 times in ice-cold purification buffer, followed by two washes with ice-cold 150 mM NaCl wash buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% [v/v] glycerol, 1% [v/v] Triton X-100, 1 mM EDTA). ..

    Fluorescence In Situ Hybridization:

    Article Title: Her9/Hes4 is required for retinal photoreceptor development, maintenance, and survival
    Article Snippet: HRMA was performed using the LightCycler 480 High-Resolution Melting Master (Roche) kit according to the manufacturer's instructions on a LightCycler 96 Real-Time PCR System (Roche). .. Genomic DNA (gDNA) extraction and amplificationgDNA was extracted from whole embryos or from tail clips of adult fish. .. The embryos or tails were placed in 20 µl of 1 × Thermopol buffer (NEB, Ipswich, MA) and incubated at 95°C to soften the tissue.

    Isolation:

    Article Title: Reduced chlorhexidine and daptomycin susceptibility arises in vancomycin-resistant Enterococcus faecium after serial chlorhexidine exposure
    Article Snippet: .. Routine molecular biology techniques E. faecium genomic DNA (gDNA) was isolated using a previously published protocol ( ). .. DNA fragments were purified using the Purelink PCR purification kit (Thermo Fisher).

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    New England Biolabs monarch gdna wash buffer
    Schematic diagram of the pipeline used to quantify individual species in a mixed biofilm. Samples are PMA (propidium monoazide) treated to ensure quantification of genomic <t>DNA</t> only from live cells. After treatment, cells are lysed, DNA is extracted, followed by qPCR quantification of DNA and subsequent conversion to bacterial numbers.
    Monarch Gdna Wash Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch gdna wash buffer/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna wash buffer - by Bioz Stars, 2021-05
    96/100 stars
      Buy from Supplier

    Image Search Results


    Schematic diagram of the pipeline used to quantify individual species in a mixed biofilm. Samples are PMA (propidium monoazide) treated to ensure quantification of genomic DNA only from live cells. After treatment, cells are lysed, DNA is extracted, followed by qPCR quantification of DNA and subsequent conversion to bacterial numbers.

    Journal: bioRxiv

    Article Title: Dissecting individual pathogen-commensal interactions within a complex gut microbiota community

    doi: 10.1101/2020.04.06.027128

    Figure Lengend Snippet: Schematic diagram of the pipeline used to quantify individual species in a mixed biofilm. Samples are PMA (propidium monoazide) treated to ensure quantification of genomic DNA only from live cells. After treatment, cells are lysed, DNA is extracted, followed by qPCR quantification of DNA and subsequent conversion to bacterial numbers.

    Article Snippet: Genomic DNA extraction DNA extraction was carried out using a phenol chloroform-based method.

    Techniques: Real-time Polymerase Chain Reaction

    Clustered regularly interspaced short palindromic repeats (CRISPR) RNA (crRNA) was designed with complementary nucleotides adjacent to the protospacer adjacent motif (PAM) to target CRISPR-associated protein 9 (Cas9) endonuclease to the correct DNA sequence within genomic DNA. HNH (an endonuclease domain named for characteristic histidine and asparagine residues) and RuvC-like nuclease domains cut the target DNA 3 nucleotides upstream of the PAM (NGG) sequence leading to the formation of a double-strand break. Mutations were inserted utilizing a homology-directed repair (HDR) pathway to repair the dsDNA breaks using 98 nt single-strand oligodeoxynucleotide (ODN) sequence as a template with 40–50 nucleotides flanking the dsDNA break site to introduce point mutations (PM).

    Journal: Biosensors

    Article Title: Detection of CRISPR-Cas9-Mediated Mutations Using a Carbon Nanotube-Modified Electrochemical Genosensor

    doi: 10.3390/bios11010017

    Figure Lengend Snippet: Clustered regularly interspaced short palindromic repeats (CRISPR) RNA (crRNA) was designed with complementary nucleotides adjacent to the protospacer adjacent motif (PAM) to target CRISPR-associated protein 9 (Cas9) endonuclease to the correct DNA sequence within genomic DNA. HNH (an endonuclease domain named for characteristic histidine and asparagine residues) and RuvC-like nuclease domains cut the target DNA 3 nucleotides upstream of the PAM (NGG) sequence leading to the formation of a double-strand break. Mutations were inserted utilizing a homology-directed repair (HDR) pathway to repair the dsDNA breaks using 98 nt single-strand oligodeoxynucleotide (ODN) sequence as a template with 40–50 nucleotides flanking the dsDNA break site to introduce point mutations (PM).

    Article Snippet: Genomic DNA (gDNA) was extracted using 50 μL of QuickExtract™ DNA extraction solution (Epicentre-Lucigen).

    Techniques: CRISPR, Sequencing, Introduce