gdna lysis buffer (New England Biolabs)


93
Structured Review
New England Biolabs
gdna lysis buffer
Gdna Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gdna lysis buffer/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Gdna Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gdna lysis buffer/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gdna lysis buffer - by Bioz Stars,
2023-02
93/100 stars
Images
gdna lysis buffer (New England Biolabs)


93
Structured Review
New England Biolabs
gdna lysis buffer
Gdna Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gdna lysis buffer/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Gdna Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gdna lysis buffer/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gdna lysis buffer - by Bioz Stars,
2023-02
93/100 stars
Images
gdna lysis buffer (New England Biolabs)


93
Structured Review
New England Biolabs
gdna lysis buffer
Figure 1 (A) Presumed model by which the reverse-transcribed 3′ DNA flap is permanently incorporated into the genome during prime editing ( Anzalone et al., 2019 ). (B) Installation of a G⋅C-to-C⋅G edit within a lentivirally integrated HBB sequence using SaPE2 and Sa-pegRNAs in HEK293T cells. PBS, primer binding site. Data represent the mean of n = 3 independent replicates. (C) Design of the prime editing Repair-seq lentiviral vector (pPC1000, additional details and full sequence information in ). In Repair-seq screens, a 453-bp region containing CRISPRi sgRNA sequence and prime editing outcome is amplified from genomic DNA for paired-end Illumina sequencing. The CRISPRi sgRNA is sequenced with a 44-nt Illumina forward read (R1), and the prime edited site (including +50 and –50 nick sites) is sequenced with a 263-nt Illumina reverse read (R2). Black triangles indicate positions of SaPE2-induced nicks programmed by Sa-pegRNA and Sa-sgRNAs. Sizes of all vector components are to scale. (D) Schematic of PE2, PE3+50, and PE3–50 prime editing configurations with SaPE2 protein (SaCas9 N580A fused to an engineered MMLV RT). (E) Validation of intended G⋅C-to-C⋅G editing at the lentivirally integrated Repair-seq edit site in HeLa cells expressing dCas9–BFP–KRAB cells. Bars represent the mean of n = 2 independent replicates. (F) Prime editing at the Repair-seq edit site with blasticidin selection in HeLa cells expressing dCas9–BFP–KRAB. SaPE2–P2A–BlastR prime editor was used for all conditions. Bars represent the mean of n = 2 independent replicates. (G) Functional annotation classes of the genes targeted by the pooled CRISPRi sgRNA library used in Repair-seq screens. (H–K) Knockdown of MSH2 , MSH6 , MLH1 , and PMS2 increases the frequency of the intended +6 G⋅C-to-C⋅G prime edit in all Repair-seq screens. Dots represent individual CRISPRi sgRNAs. " width="250" height="auto" />
Gdna Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gdna lysis buffer/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

Gdna Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gdna lysis buffer/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gdna lysis buffer - by Bioz Stars,
2023-02
93/100 stars
Images
1) Product Images from "Enhanced prime editing systems by manipulating cellular determinants of editing outcomes"
Article Title: Enhanced prime editing systems by manipulating cellular determinants of editing outcomes
Journal: Cell
doi: 10.1016/j.cell.2021.09.018

Figure Legend Snippet: Design and results of Repair-seq screens for substitution prime editing outcomes, related to
Techniques Used: Sequencing, Binding Assay, Plasmid Preparation, Amplification, Expressing, Selection, Functional Assay