monarch gdna blood lysis buffer  (New England Biolabs)


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    Name:
    Monarch gDNA Blood Lysis Buffer
    Description:
    The Monarch gDNA Blood Lysis Buffer a component of the Monarch Genomic DNA Purification Kit The Blood Lysis Buffer contains a strong protective component against the high nuclease activity in blood samples and supports rapid degradation of hemoglobin and other protein components
    Catalog Number:
    T3013L
    Price:
    40
    Category:
    Genomic DNA Purification Kit Components
    Size:
    20 ml
    		Buy from Supplier

    Structured Review

    New England Biolabs monarch gdna blood lysis buffer
    Monarch gDNA Blood Lysis Buffer
    The Monarch gDNA Blood Lysis Buffer a component of the Monarch Genomic DNA Purification Kit The Blood Lysis Buffer contains a strong protective component against the high nuclease activity in blood samples and supports rapid degradation of hemoglobin and other protein components
    https://www.bioz.com/result/monarch gdna blood lysis buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna blood lysis buffer - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes"

    Article Title: Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes

    Journal: bioRxiv

    doi: 10.1101/2020.04.03.022038

    SHK-1 cells were electroporated with 1.4 μM Cas9:gRNA RNP and transferred to 2 separate 96-well plates. (A) After 48h, cell survival (plate1) was calculated using CellTiter Glo 2.0. (B) genomic DNA (plate 2) was extracted at 7 dpt, and the target sequence amplified by PCR and editing efficiency estimated using Sanger sequencing. (C) Using 1.4 μM RNP, the editing efficiency after 7 and 14 ddpt was estimated for different electroporation settings. (D) All the sequencing data, obtained from ICE analysis of Sanger sequencing of the intergenic target region from optimisation experiments (n=55) were pooled and plotted according to edit pattern.
    Figure Legend Snippet: SHK-1 cells were electroporated with 1.4 μM Cas9:gRNA RNP and transferred to 2 separate 96-well plates. (A) After 48h, cell survival (plate1) was calculated using CellTiter Glo 2.0. (B) genomic DNA (plate 2) was extracted at 7 dpt, and the target sequence amplified by PCR and editing efficiency estimated using Sanger sequencing. (C) Using 1.4 μM RNP, the editing efficiency after 7 and 14 ddpt was estimated for different electroporation settings. (D) All the sequencing data, obtained from ICE analysis of Sanger sequencing of the intergenic target region from optimisation experiments (n=55) were pooled and plotted according to edit pattern.

    Techniques Used: Sequencing, Amplification, Polymerase Chain Reaction, Electroporation

    2) Product Images from "Functional characterization of the idtF and idtP genes in the Claviceps paspali indole diterpene biosynthetic gene cluster"

    Article Title: Functional characterization of the idtF and idtP genes in the Claviceps paspali indole diterpene biosynthetic gene cluster

    Journal: Folia Microbiologica

    doi: 10.1007/s12223-020-00777-6

    Construction of the pAg- idtF -KO and pAg- idtP -KO vectors. The left and right targeting arms (LTA and RTA, respectively) for the idtF and idtP genes, respectively, were PCR amplified from the genomic DNA of the C. paspali DSM833 strain. The backbone of the pAg-H3 plasmid (without the hph gene) and the hph gene alone were amplified separately and fused with the appropriate LTA and RTA amplicons using Gibson assembly. Gene symbols: hph , hygromycin phosphotransferase; nptII : neomycin phosphotransferase II (kanamycin resistance); trfA , plasmid replication initiation gene; oriV, vegetative origin of replication; LB and RB, left and right T-DNA border, respectively
    Figure Legend Snippet: Construction of the pAg- idtF -KO and pAg- idtP -KO vectors. The left and right targeting arms (LTA and RTA, respectively) for the idtF and idtP genes, respectively, were PCR amplified from the genomic DNA of the C. paspali DSM833 strain. The backbone of the pAg-H3 plasmid (without the hph gene) and the hph gene alone were amplified separately and fused with the appropriate LTA and RTA amplicons using Gibson assembly. Gene symbols: hph , hygromycin phosphotransferase; nptII : neomycin phosphotransferase II (kanamycin resistance); trfA , plasmid replication initiation gene; oriV, vegetative origin of replication; LB and RB, left and right T-DNA border, respectively

    Techniques Used: Polymerase Chain Reaction, Amplification, Plasmid Preparation

    Related Articles

    other:

    Article Title: Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration.
    Article Snippet: Conventional CRISPR-Cas systems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mobile genetic elements, including plasmids and viruses.

    Article Title: From iron to antibiotics: Identification of conserved bacterial-fungal interactions across diverse partners
    Article Snippet: After gDNA extraction, extracts containing Penicillium sp. str.

    Article Title: Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes
    Article Snippet: Genomic DNA (gDNA) was extracted with QuickExtract buffer (Lucigen, Middleton, USA) by adding 30 μL to a well of a 96-well plate and incubating for 5 min.

    Sample Prep:

    Article Title: Functional characterization of the idtF and idtP genes in the Claviceps paspali indole diterpene biosynthetic gene cluster
    Article Snippet: .. The maintenance of the fungus, fermentation conditions, sample preparation for IDT analysis, and genomic DNA isolation was carried out as describer earlier (Kozák et al. ). .. PCR reactions were carried out in 50 μL total volumes, containing 20 ng genomic DNA, or 1 ng plasmid DNA as the template, respectively; 0.2 mmol/L of each dNTP; 1 pmol/L of each primer; 1 μL Phusion® High-Fidelity DNA Polymerase; and 10 μL HF buffer (New England Biolabs, Ipswich, MA).

    DNA Extraction:

    Article Title: Functional characterization of the idtF and idtP genes in the Claviceps paspali indole diterpene biosynthetic gene cluster
    Article Snippet: .. The maintenance of the fungus, fermentation conditions, sample preparation for IDT analysis, and genomic DNA isolation was carried out as describer earlier (Kozák et al. ). .. PCR reactions were carried out in 50 μL total volumes, containing 20 ng genomic DNA, or 1 ng plasmid DNA as the template, respectively; 0.2 mmol/L of each dNTP; 1 pmol/L of each primer; 1 μL Phusion® High-Fidelity DNA Polymerase; and 10 μL HF buffer (New England Biolabs, Ipswich, MA).

    Marker:

    Article Title: Bean common mosaic virus Isolate Exhibits a Novel Pathogenicity Profile in Common Bean, Overcoming the bc-3 Resistance Allele Coding for the Mutated eIF4E Translation Initiation Factor.
    Article Snippet: .. Further analysis was conducted with the Recombination Detection Program v.4.16 (RDP4) (Martin et al. 2005). gDNA extraction, CAPS marker eIF4E-RsaI analysis, and eIF4E sequencing. .. Genomic DNA (gDNA) was extracted from 0.1 g of young leaf tissue using the CTAB methodology as described in Naderpour et al. (2010).

    Sequencing:

    Article Title: Bean common mosaic virus Isolate Exhibits a Novel Pathogenicity Profile in Common Bean, Overcoming the bc-3 Resistance Allele Coding for the Mutated eIF4E Translation Initiation Factor.
    Article Snippet: .. Further analysis was conducted with the Recombination Detection Program v.4.16 (RDP4) (Martin et al. 2005). gDNA extraction, CAPS marker eIF4E-RsaI analysis, and eIF4E sequencing. .. Genomic DNA (gDNA) was extracted from 0.1 g of young leaf tissue using the CTAB methodology as described in Naderpour et al. (2010).

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    • monarch gdna blood lysis buffer  (New England Biolabs)
    93
    New England Biolabs monarch gdna blood lysis buffer
    SHK-1 cells were electroporated with 1.4 μM Cas9:gRNA RNP and transferred to 2 separate 96-well plates. (A) After 48h, cell survival (plate1) was calculated using CellTiter Glo 2.0. (B) genomic <t>DNA</t> (plate 2) was extracted at 7 dpt, and the target sequence amplified by PCR and editing efficiency estimated using Sanger sequencing. (C) Using 1.4 μM RNP, the editing efficiency after 7 and 14 ddpt was estimated for different electroporation settings. (D) All the sequencing data, obtained from ICE analysis of Sanger sequencing of the intergenic target region from optimisation experiments (n=55) were pooled and plotted according to edit pattern.
    Monarch Gdna Blood Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch gdna blood lysis buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna blood lysis buffer - by Bioz Stars, 2021-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    SHK-1 cells were electroporated with 1.4 μM Cas9:gRNA RNP and transferred to 2 separate 96-well plates. (A) After 48h, cell survival (plate1) was calculated using CellTiter Glo 2.0. (B) genomic DNA (plate 2) was extracted at 7 dpt, and the target sequence amplified by PCR and editing efficiency estimated using Sanger sequencing. (C) Using 1.4 μM RNP, the editing efficiency after 7 and 14 ddpt was estimated for different electroporation settings. (D) All the sequencing data, obtained from ICE analysis of Sanger sequencing of the intergenic target region from optimisation experiments (n=55) were pooled and plotted according to edit pattern.

    Journal: bioRxiv

    Article Title: Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes

    doi: 10.1101/2020.04.03.022038

    Figure Lengend Snippet: SHK-1 cells were electroporated with 1.4 μM Cas9:gRNA RNP and transferred to 2 separate 96-well plates. (A) After 48h, cell survival (plate1) was calculated using CellTiter Glo 2.0. (B) genomic DNA (plate 2) was extracted at 7 dpt, and the target sequence amplified by PCR and editing efficiency estimated using Sanger sequencing. (C) Using 1.4 μM RNP, the editing efficiency after 7 and 14 ddpt was estimated for different electroporation settings. (D) All the sequencing data, obtained from ICE analysis of Sanger sequencing of the intergenic target region from optimisation experiments (n=55) were pooled and plotted according to edit pattern.

    Article Snippet: Genomic DNA (gDNA) was extracted with QuickExtract buffer (Lucigen, Middleton, USA) by adding 30 μL to a well of a 96-well plate and incubating for 5 min.

    Techniques: Sequencing, Amplification, Polymerase Chain Reaction, Electroporation

    Construction of the pAg- idtF -KO and pAg- idtP -KO vectors. The left and right targeting arms (LTA and RTA, respectively) for the idtF and idtP genes, respectively, were PCR amplified from the genomic DNA of the C. paspali DSM833 strain. The backbone of the pAg-H3 plasmid (without the hph gene) and the hph gene alone were amplified separately and fused with the appropriate LTA and RTA amplicons using Gibson assembly. Gene symbols: hph , hygromycin phosphotransferase; nptII : neomycin phosphotransferase II (kanamycin resistance); trfA , plasmid replication initiation gene; oriV, vegetative origin of replication; LB and RB, left and right T-DNA border, respectively

    Journal: Folia Microbiologica

    Article Title: Functional characterization of the idtF and idtP genes in the Claviceps paspali indole diterpene biosynthetic gene cluster

    doi: 10.1007/s12223-020-00777-6

    Figure Lengend Snippet: Construction of the pAg- idtF -KO and pAg- idtP -KO vectors. The left and right targeting arms (LTA and RTA, respectively) for the idtF and idtP genes, respectively, were PCR amplified from the genomic DNA of the C. paspali DSM833 strain. The backbone of the pAg-H3 plasmid (without the hph gene) and the hph gene alone were amplified separately and fused with the appropriate LTA and RTA amplicons using Gibson assembly. Gene symbols: hph , hygromycin phosphotransferase; nptII : neomycin phosphotransferase II (kanamycin resistance); trfA , plasmid replication initiation gene; oriV, vegetative origin of replication; LB and RB, left and right T-DNA border, respectively

    Article Snippet: The maintenance of the fungus, fermentation conditions, sample preparation for IDT analysis, and genomic DNA isolation was carried out as describer earlier (Kozák et al. ).

    Techniques: Polymerase Chain Reaction, Amplification, Plasmid Preparation