gdna lysis buffer  (New England Biolabs)


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    Name:
    Monarch gDNA Tissue Lysis Buffer
    Description:
    The Monarch gDNA Tissue Lysis Buffer is a component of the Monarch Genomic DNA Purification Kit The Tissue Lysis Buffer supplies intermediate lysis conditions that enable rapid digestion of the tissue pieces while simultaneously ensuring the genomic DNA fragment length is optimal for binding and elution
    Catalog Number:
    T3011L
    Price:
    40
    Category:
    Genomic DNA Purification Kit Components
    Size:
    34 ml
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    Structured Review

    New England Biolabs gdna lysis buffer
    Monarch gDNA Tissue Lysis Buffer
    The Monarch gDNA Tissue Lysis Buffer is a component of the Monarch Genomic DNA Purification Kit The Tissue Lysis Buffer supplies intermediate lysis conditions that enable rapid digestion of the tissue pieces while simultaneously ensuring the genomic DNA fragment length is optimal for binding and elution
    https://www.bioz.com/result/gdna lysis buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdna lysis buffer - by Bioz Stars, 2021-05
    93/100 stars

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    Related Articles

    Methylation:

    Article Title: lncRNA MEG8 Upregulates miR-770-5p Through Methylation and Promotes Cell Apoptosis in Diabetic Nephropathy
    Article Snippet: Finally, flow cytometry was used to analyze apoptotic (early apoptosis) cells. .. Methylation-Specific PCR (MSP) CIHP-1 cells were subjected to genomic DNA isolation using Monarch® Genomic DNA Purification Kit (NEB). .. EZ DNA Methylation-GoldTM kit (ZYMO) was used to convert DNA samples.

    Polymerase Chain Reaction:

    Article Title: lncRNA MEG8 Upregulates miR-770-5p Through Methylation and Promotes Cell Apoptosis in Diabetic Nephropathy
    Article Snippet: Finally, flow cytometry was used to analyze apoptotic (early apoptosis) cells. .. Methylation-Specific PCR (MSP) CIHP-1 cells were subjected to genomic DNA isolation using Monarch® Genomic DNA Purification Kit (NEB). .. EZ DNA Methylation-GoldTM kit (ZYMO) was used to convert DNA samples.

    DNA Extraction:

    Article Title: lncRNA MEG8 Upregulates miR-770-5p Through Methylation and Promotes Cell Apoptosis in Diabetic Nephropathy
    Article Snippet: Finally, flow cytometry was used to analyze apoptotic (early apoptosis) cells. .. Methylation-Specific PCR (MSP) CIHP-1 cells were subjected to genomic DNA isolation using Monarch® Genomic DNA Purification Kit (NEB). .. EZ DNA Methylation-GoldTM kit (ZYMO) was used to convert DNA samples.

    Article Title: CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
    Article Snippet: .. DNA Extraction HEK293T cells were washed with 1X PBS (Corning) and lysed overnight by shaking at 55°C with 43.5 μL of gDNA lysis buffer (100 mM Tris-HCl at pH 8, 200 mM NaCl, 5 mM EDTA, 0.05% SDS) supplemented with 5.25 μL of 20 mg/ml Proteinase K (NEB) and 1.25 μL of 1M DTT (Sigma) per well for experiments in 96-well plates, or with 174 μL DNA lysis buffer, 21 μL Proteinase K, and 5 μL 1M DTT per well for experiments in 24-well plates. .. K562 cells were centrifuged for 5 min, media removed, and lysed overnight by shaking at 55°C with 174 μL DNA lysis buffer, 21 μL Proteinase K, and 5 μL 1M DTT per well in 24-well plates.

    DNA Purification:

    Article Title: lncRNA MEG8 Upregulates miR-770-5p Through Methylation and Promotes Cell Apoptosis in Diabetic Nephropathy
    Article Snippet: Finally, flow cytometry was used to analyze apoptotic (early apoptosis) cells. .. Methylation-Specific PCR (MSP) CIHP-1 cells were subjected to genomic DNA isolation using Monarch® Genomic DNA Purification Kit (NEB). .. EZ DNA Methylation-GoldTM kit (ZYMO) was used to convert DNA samples.

    Lysis:

    Article Title: CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
    Article Snippet: .. DNA Extraction HEK293T cells were washed with 1X PBS (Corning) and lysed overnight by shaking at 55°C with 43.5 μL of gDNA lysis buffer (100 mM Tris-HCl at pH 8, 200 mM NaCl, 5 mM EDTA, 0.05% SDS) supplemented with 5.25 μL of 20 mg/ml Proteinase K (NEB) and 1.25 μL of 1M DTT (Sigma) per well for experiments in 96-well plates, or with 174 μL DNA lysis buffer, 21 μL Proteinase K, and 5 μL 1M DTT per well for experiments in 24-well plates. .. K562 cells were centrifuged for 5 min, media removed, and lysed overnight by shaking at 55°C with 174 μL DNA lysis buffer, 21 μL Proteinase K, and 5 μL 1M DTT per well in 24-well plates.

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  • 93
    New England Biolabs gdna lysis buffer
    Gdna Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdna lysis buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdna lysis buffer - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

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