monarch dna purification kit  (New England Biolabs)


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    Name:
    Monarch Genomic DNA Purification Kit
    Description:
    The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
    Catalog Number:
    t3010l
    Price:
    395
    Size:
    150 preps
    Category:
    Genomic DNA Purification Kits
    Buy from Supplier


    Structured Review

    New England Biolabs monarch dna purification kit
    Monarch Genomic DNA Purification Kit
    The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
    https://www.bioz.com/result/monarch dna purification kit/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    monarch dna purification kit - by Bioz Stars, 2020-05
    94/100 stars

    Related Products / Commonly Used Together

    be104
    ssdna
    genomic dna gdna
    non-endemic whole blood

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Rates and Microbial Players of Iron-Driven Anaerobic Oxidation of Methane in Methanic Marine Sediments
    Article Snippet: .. PCR cycling conditions include: 95°C: 5 min; 28 cycles at 98°C: 20 s, 60°C: 20 s, 72°C: 20 s; 72°C: 1 min. PCR products were screened by gel electrophoresis as mentioned before and purified using Monarch® PCR & DNA purification kit (New England Biolabs, Germany). .. PCR products were quantified using Quant-iT PicoGreen dsDNA assay kit (Invitrogen-Thermo Fischer Scientific, Steinheim, Germany).

    Isolation:

    Article Title: An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms
    Article Snippet: .. Reaction products were pooled in two samples and ssDNA isolated using NEB Monarch DNA purification kit following the manufacturer’s instructions (NEB #T1030L). .. Samples were isolated in 2 × 10 μl prewarmed (55°C) elution buffer from the kit and the DNA concentration was measured using NanoDrop One (ThermoFisher Scientific).

    Article Title: Complete Genome Sequence of Escherichia coli BE104, an MC4100 Derivative Lacking the Methionine Reductive Pathway
    Article Snippet: .. BE104 was grown in standard rich medium (10 g/liter tryptone, 5 g/liter yeast extract, 5 g/liter NaCl, NaOH [pH 7.2]) at 30°C, and its genomic DNA (gDNA) was isolated using the Monarch gDNA kit (New England BioLabs). .. A SMRTbell library was constructed from 5 μg gDNA and sheared to ∼10 kb using a g-TUBE (Covaris).

    Nucleic Acid Electrophoresis:

    Article Title: Rates and Microbial Players of Iron-Driven Anaerobic Oxidation of Methane in Methanic Marine Sediments
    Article Snippet: .. PCR cycling conditions include: 95°C: 5 min; 28 cycles at 98°C: 20 s, 60°C: 20 s, 72°C: 20 s; 72°C: 1 min. PCR products were screened by gel electrophoresis as mentioned before and purified using Monarch® PCR & DNA purification kit (New England Biolabs, Germany). .. PCR products were quantified using Quant-iT PicoGreen dsDNA assay kit (Invitrogen-Thermo Fischer Scientific, Steinheim, Germany).

    DNA Purification:

    Article Title: In Silico Identification of Novel Biomarkers and Development of New Rapid Diagnostic Tests for the Filarial Parasites Mansonella perstans and Mansonella ozzardi
    Article Snippet: .. DNA was prepared from non-endemic whole blood samples using the Monarch Genomic DNA Purification Kit (New England Biolabs) as directed by the manufacturer. .. These genomic DNAs were used in all LAMP and nested PCR assays.

    Article Title: An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms
    Article Snippet: .. Reaction products were pooled in two samples and ssDNA isolated using NEB Monarch DNA purification kit following the manufacturer’s instructions (NEB #T1030L). .. Samples were isolated in 2 × 10 μl prewarmed (55°C) elution buffer from the kit and the DNA concentration was measured using NanoDrop One (ThermoFisher Scientific).

    Article Title: Rates and Microbial Players of Iron-Driven Anaerobic Oxidation of Methane in Methanic Marine Sediments
    Article Snippet: .. PCR cycling conditions include: 95°C: 5 min; 28 cycles at 98°C: 20 s, 60°C: 20 s, 72°C: 20 s; 72°C: 1 min. PCR products were screened by gel electrophoresis as mentioned before and purified using Monarch® PCR & DNA purification kit (New England Biolabs, Germany). .. PCR products were quantified using Quant-iT PicoGreen dsDNA assay kit (Invitrogen-Thermo Fischer Scientific, Steinheim, Germany).

    Article Title: Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B
    Article Snippet: .. DNA fragment were purified from agarose gels using Monarch DNA purification kit (New England Biolabs). .. DNA sequencing was performed by GATC Biotech (Konstanz, Germany).

    Purification:

    Article Title: Rates and Microbial Players of Iron-Driven Anaerobic Oxidation of Methane in Methanic Marine Sediments
    Article Snippet: .. PCR cycling conditions include: 95°C: 5 min; 28 cycles at 98°C: 20 s, 60°C: 20 s, 72°C: 20 s; 72°C: 1 min. PCR products were screened by gel electrophoresis as mentioned before and purified using Monarch® PCR & DNA purification kit (New England Biolabs, Germany). .. PCR products were quantified using Quant-iT PicoGreen dsDNA assay kit (Invitrogen-Thermo Fischer Scientific, Steinheim, Germany).

    Article Title: Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B
    Article Snippet: .. DNA fragment were purified from agarose gels using Monarch DNA purification kit (New England Biolabs). .. DNA sequencing was performed by GATC Biotech (Konstanz, Germany).

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  • Bioz Stars
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  • 94
    New England Biolabs monarch dna purification kit
    Monarch Dna Purification Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch dna purification kit/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    monarch dna purification kit - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

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