pbst  (New England Biolabs)


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    Structured Review

    New England Biolabs pbst
    Pbst, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-02
    90/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The sample was then homogenized on ice for 30 s using a Polytron homogenizer at slow speed before being mixed with lysis buffer (protease inhibitor cocktail, 10 mmol/L β ‐glycerophosphate, 50 mmol/L sodium fluoride, and 0.5 mmol/L sodium orthovanadate) and allowed to stand on ice for 2 h. “Debris” containing the nuclei was removed by centrifugation at 10,000 g at 4°C for 10 min. A bicinchoninic acid (BCA) assay was then used to determine the protein concentration of the homogenate in order that the sample contained a known concentration of 2 μ g of protein/μ L. The sample was made up from protein, homogenizing buffer, and Laemmli SDS Buffer (3.78 g [30%] glycerol, 2.6 mL 0.625 M Tris buffer, 3 mL 20% sodium dodecyl sulfate, 0.5 mL 0.5% bromophenol blue, 0.12 mL deionized water, 100 μ L of β ‐mercaptoethanol per 900 μ L of sample buffer). .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Fluorescence:

    Article Title: Protein evolution with an expanded genetic code
    Article Snippet: .. After blocking for 2 h with 200 μl 2% milk (Bio-Rad) in PBS, equal amounts of concentrated phage previously expressed, precipitated, and quantified by ELISA according to the procedures described above, were loaded in 100 μl 2% milk PBST and incubated at 37 °C for 2 h. After washing five times with PBST, an anti-M13 polyclonal antibody (NEB) was added in 110 μl of 2% milk in PBST and incubated at 37 °C for 2 h. After washing eight times with PBST, QuantaBlu fluorogenic substrate (Pierce) was added and the ELISA signal was determined using a fluorescence plate reader (SpectraMax Gemini). ..

    Homogenization:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: Snap frozen muscle tissue was powdered in liquid nitrogen and transferred to Eppendorf tubes containing homogenization buffer (50 mmol/L Tris‐HCl, 1 mmol/L EDTA, 1 mmol/L EGTA and 1% Triton X‐100 in deionized water). .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Staining:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: Successful transfer was confirmed using Ponceau S staining (Sigma Aldrich) for 5 min before washing for 1 min in 0.1 M sodium hydroxide to destain. .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Protease Inhibitor:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The sample was then homogenized on ice for 30 s using a Polytron homogenizer at slow speed before being mixed with lysis buffer (protease inhibitor cocktail, 10 mmol/L β ‐glycerophosphate, 50 mmol/L sodium fluoride, and 0.5 mmol/L sodium orthovanadate) and allowed to stand on ice for 2 h. “Debris” containing the nuclei was removed by centrifugation at 10,000 g at 4°C for 10 min. A bicinchoninic acid (BCA) assay was then used to determine the protein concentration of the homogenate in order that the sample contained a known concentration of 2 μ g of protein/μ L. The sample was made up from protein, homogenizing buffer, and Laemmli SDS Buffer (3.78 g [30%] glycerol, 2.6 mL 0.625 M Tris buffer, 3 mL 20% sodium dodecyl sulfate, 0.5 mL 0.5% bromophenol blue, 0.12 mL deionized water, 100 μ L of β ‐mercaptoethanol per 900 μ L of sample buffer). .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    BIA-KA:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The sample was then homogenized on ice for 30 s using a Polytron homogenizer at slow speed before being mixed with lysis buffer (protease inhibitor cocktail, 10 mmol/L β ‐glycerophosphate, 50 mmol/L sodium fluoride, and 0.5 mmol/L sodium orthovanadate) and allowed to stand on ice for 2 h. “Debris” containing the nuclei was removed by centrifugation at 10,000 g at 4°C for 10 min. A bicinchoninic acid (BCA) assay was then used to determine the protein concentration of the homogenate in order that the sample contained a known concentration of 2 μ g of protein/μ L. The sample was made up from protein, homogenizing buffer, and Laemmli SDS Buffer (3.78 g [30%] glycerol, 2.6 mL 0.625 M Tris buffer, 3 mL 20% sodium dodecyl sulfate, 0.5 mL 0.5% bromophenol blue, 0.12 mL deionized water, 100 μ L of β ‐mercaptoethanol per 900 μ L of sample buffer). .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Western Blot:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: Paragraph title: Western blot analysis ... The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Blocking Assay:

    Article Title: Protein evolution with an expanded genetic code
    Article Snippet: .. After blocking for 2 h with 200 μl 2% milk (Bio-Rad) in PBS, equal amounts of concentrated phage previously expressed, precipitated, and quantified by ELISA according to the procedures described above, were loaded in 100 μl 2% milk PBST and incubated at 37 °C for 2 h. After washing five times with PBST, an anti-M13 polyclonal antibody (NEB) was added in 110 μl of 2% milk in PBST and incubated at 37 °C for 2 h. After washing eight times with PBST, QuantaBlu fluorogenic substrate (Pierce) was added and the ELISA signal was determined using a fluorescence plate reader (SpectraMax Gemini). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Protein evolution with an expanded genetic code
    Article Snippet: .. After blocking for 2 h with 200 μl 2% milk (Bio-Rad) in PBS, equal amounts of concentrated phage previously expressed, precipitated, and quantified by ELISA according to the procedures described above, were loaded in 100 μl 2% milk PBST and incubated at 37 °C for 2 h. After washing five times with PBST, an anti-M13 polyclonal antibody (NEB) was added in 110 μl of 2% milk in PBST and incubated at 37 °C for 2 h. After washing eight times with PBST, QuantaBlu fluorogenic substrate (Pierce) was added and the ELISA signal was determined using a fluorescence plate reader (SpectraMax Gemini). ..

    Concentration Assay:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The sample was then homogenized on ice for 30 s using a Polytron homogenizer at slow speed before being mixed with lysis buffer (protease inhibitor cocktail, 10 mmol/L β ‐glycerophosphate, 50 mmol/L sodium fluoride, and 0.5 mmol/L sodium orthovanadate) and allowed to stand on ice for 2 h. “Debris” containing the nuclei was removed by centrifugation at 10,000 g at 4°C for 10 min. A bicinchoninic acid (BCA) assay was then used to determine the protein concentration of the homogenate in order that the sample contained a known concentration of 2 μ g of protein/μ L. The sample was made up from protein, homogenizing buffer, and Laemmli SDS Buffer (3.78 g [30%] glycerol, 2.6 mL 0.625 M Tris buffer, 3 mL 20% sodium dodecyl sulfate, 0.5 mL 0.5% bromophenol blue, 0.12 mL deionized water, 100 μ L of β ‐mercaptoethanol per 900 μ L of sample buffer). .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Incubation:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST. .. Following three 5 min washes in PBST, membranes were then incubated for 1 h in 5% NFDM in PBST followed by a 1 h incubation in an appropriately targeted horseradish peroxidase–linked secondary antibody in 5% NFDM in PBST.

    Article Title: Protein evolution with an expanded genetic code
    Article Snippet: .. After blocking for 2 h with 200 μl 2% milk (Bio-Rad) in PBS, equal amounts of concentrated phage previously expressed, precipitated, and quantified by ELISA according to the procedures described above, were loaded in 100 μl 2% milk PBST and incubated at 37 °C for 2 h. After washing five times with PBST, an anti-M13 polyclonal antibody (NEB) was added in 110 μl of 2% milk in PBST and incubated at 37 °C for 2 h. After washing eight times with PBST, QuantaBlu fluorogenic substrate (Pierce) was added and the ELISA signal was determined using a fluorescence plate reader (SpectraMax Gemini). ..

    Imaging:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST. .. Antibody binding was detected using enhanced chemiluminescence HRP detection reagent (GE Healthcare, Little Chalfont, Bucks, UK) and imaging was performed using Chemi Doc software (Bio‐Rad, Hemel Hempsted, Herts, UK).

    Protein Concentration:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The sample was then homogenized on ice for 30 s using a Polytron homogenizer at slow speed before being mixed with lysis buffer (protease inhibitor cocktail, 10 mmol/L β ‐glycerophosphate, 50 mmol/L sodium fluoride, and 0.5 mmol/L sodium orthovanadate) and allowed to stand on ice for 2 h. “Debris” containing the nuclei was removed by centrifugation at 10,000 g at 4°C for 10 min. A bicinchoninic acid (BCA) assay was then used to determine the protein concentration of the homogenate in order that the sample contained a known concentration of 2 μ g of protein/μ L. The sample was made up from protein, homogenizing buffer, and Laemmli SDS Buffer (3.78 g [30%] glycerol, 2.6 mL 0.625 M Tris buffer, 3 mL 20% sodium dodecyl sulfate, 0.5 mL 0.5% bromophenol blue, 0.12 mL deionized water, 100 μ L of β ‐mercaptoethanol per 900 μ L of sample buffer). .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Lysis:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The sample was then homogenized on ice for 30 s using a Polytron homogenizer at slow speed before being mixed with lysis buffer (protease inhibitor cocktail, 10 mmol/L β ‐glycerophosphate, 50 mmol/L sodium fluoride, and 0.5 mmol/L sodium orthovanadate) and allowed to stand on ice for 2 h. “Debris” containing the nuclei was removed by centrifugation at 10,000 g at 4°C for 10 min. A bicinchoninic acid (BCA) assay was then used to determine the protein concentration of the homogenate in order that the sample contained a known concentration of 2 μ g of protein/μ L. The sample was made up from protein, homogenizing buffer, and Laemmli SDS Buffer (3.78 g [30%] glycerol, 2.6 mL 0.625 M Tris buffer, 3 mL 20% sodium dodecyl sulfate, 0.5 mL 0.5% bromophenol blue, 0.12 mL deionized water, 100 μ L of β ‐mercaptoethanol per 900 μ L of sample buffer). .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Binding Assay:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST. .. Antibody binding was detected using enhanced chemiluminescence HRP detection reagent (GE Healthcare, Little Chalfont, Bucks, UK) and imaging was performed using Chemi Doc software (Bio‐Rad, Hemel Hempsted, Herts, UK).

    Molecular Weight:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: Western blot analysis Western blot analysis was performed as part of the antibody validation procedure to confirm the presence of a band at the correct molecular weight for SNAP23. .. The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST.

    Software:

    Article Title: Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
    Article Snippet: The membrane was blocked in 5% nonfat dried milk (NFDM) in PBST (New England Biolabs, Hertfordshire, UK) for 1 h at room temperature and following three 5 min washes in PBST (PBS in 1% tween), the membrane was then incubated overnight at 4°C in anti‐SNAP23 in 5% NFDM in PBST. .. Antibody binding was detected using enhanced chemiluminescence HRP detection reagent (GE Healthcare, Little Chalfont, Bucks, UK) and imaging was performed using Chemi Doc software (Bio‐Rad, Hemel Hempsted, Herts, UK).

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    The Monarch RNA Cleanup Columns 10 µg are a component of the Monarch RNA Cleanup Kit 10 µg NEB T2030 and can be used to purify and concentrate up to
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    New England Biolabs pbst
    Pbst, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

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